JPS60224421A - Cap for culture of mushroom (maitake) - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a cultivation bottle cap suitable for use in bottle cultivation of maitake mushrooms using a culture medium mainly consisting of sawdust.

The major characteristics of container cultivation of maitake mushrooms that differ from container cultivation of enokitake mushrooms, etc. are: (a) Maitake mushrooms are extremely susceptible to various bacteria, and in addition, cultivation at relatively high temperatures such as cultivation at around 20 degrees Celsius and growth at around 18 degrees Celsius is required. and is easily attacked by bacteria.

(b) When the fruiting body grows after germination, it requires physical stimulation such as pressing its head, and the fruiting body whose head is pressed concentrates its growth power and grows in an open space. It is in.

In conventional cultivation methods, for example, culturing is performed by sealing the bottle opening of the cultivation container with a translucent coating film, and after the fruiting bodies push up this coating film, a cut is made in the coating film, and the fruit is released through the cut. There are some substances that grow outside the container (Special Publication 1987-
39604), (a) above.

I tried to satisfy the condition (b).

However, the caps on the cultivation containers used in these conventional cultivation methods were designed only to prevent the ingress of bacteria and pressure on the heads of the fruit bodies, so the seeds did not germinate at all even after the period of cultivation and ripening. Moreover, even if the seeds are germinated by applying light stimulation, they often do not grow as fruit bodies, and the germination rate is as low as about 50 to 60%.

The inventor of the present invention has made extensive efforts to improve the germination rate, and as a result, the space remaining between the surface of the culture medium in the cultivation container and the cap of the container is replaced by a partition wall at a position closer to the surface of the culture medium. The germination rate is almost 100%, and the germination rate is almost 100%, and the germination rate is almost 100%. Tamaitake mushrooms are 20 to 3 times smaller than conventional ones.
It was found that the growth rate was 0%.

By dividing the space above the culture medium surface into upper and lower chambers using a partition wall with perforated holes, (a) the lower chamber has higher humidity and less light than the upper chamber, making it less susceptible to light stimulation. When fed, it is easy to germinate, and once germinated, the environment is such that it can reliably grow as a fruiting body.

(b) The head of this fruiting body is compressed by the septum, giving it the necessary physical stimulation.

(c) The upper chamber, where the fruiting bodies grow, has lower humidity than the lower chamber, and has a large amount of light, making it a suitable environment for the growth of the fruiting bodies.Moreover, the upper and lower chambers are connected by a through hole, so the environment is Changes are small, and resistance is developed by the fruiting body growing inside the upper chamber before growing outside the container.

This is thought to be due to the following.

Therefore, the problem is how to arrange the partition wall inside the container.

That is, the partition wall is fixed in a fixed position without touching the surface of the culture medium and without moving against the pushing up of the fruiting body, and in order to prevent bacteria from entering the container during inoculation, It is required that the partition wall be of a structure that can be quickly attached and detached.

The present invention has been made in view of the above circumstances, and its purpose is to enable easy and quick attachment and detachment to cultivation containers, and to improve germination rate and yield. The main feature of this cap is to provide a cap for a maitake cultivation bottle that can be dropped into the bottle opening of a maitake cultivation bottle, and is positioned near the culture medium surface without touching the culture medium surface, thereby resisting falling and pushing up of fruit bodies. The inner camp has an inverted U-shaped folded part on the upper edge that fits tightly into the bottle opening, and a through hole with an area smaller than the surface of the culture medium is drilled in the bottom surface. It consists of an outer cap that is fitted onto the folded part and has air permeability and translucency into the bottle opening without allowing germs to enter.

Hereinafter, preferred embodiments of the present invention will be described in detail based on the accompanying drawings.

1 is a wide mouth 1β bottle made of synthetic resin;
A rib la is provided around the outer peripheral surface of the eye end.

2 is a culture medium mainly composed of sawdust, which is filled in the bottle 1.

Reference numeral 3 denotes a cylindrical inner camp opening upward, the outer diameter of which is slightly smaller than the inner diameter of the mouth of the bottle 1, and is dropped into the bottle 1.

A folded part 3a is formed on the upper edge of the inner cap 3 and has an inverted U-shape facing outward.
a to the rib 1a of the bottle 1, and the inner camp 3 is attached to the bottle 1.
is fitted into the Further, a through hole 3c having an area smaller than the surface of the culture medium 2 is bored in the bottom surface 3b of the inner cap 3.

4 is a piece of kraft paper that covers the mouth of the bottle 1, that is, the opening of the inner camp 3, and this kraft paper 4 has a through hole 4a formed approximately at its center.

Further, a transparent film 4b is attached to the through hole 4a.

Reference numeral 5 denotes an outer camp, which is fitted onto the outer peripheral surface of the folded portion 3a of the inner camp 3 to fix the craft paper 4. In addition, a through hole 5a is bored in the upper surface of the outer camp 5.
The inside of the bottle is kept at an appropriate humidity through kraft paper 4.
Light is irradiated through the film 4b, and no germs will enter the bottle l.

Thus, the space between the surface of the culture medium 2 and the lower surface of the outer cap 5 is partitioned into two upper and lower chambers by the bottom surface 3b of the inner camp 3, and the upper chamber A and lower chamber B communicate with each other through the through hole 3c.

In addition, the inner cap 3 and the outer cap 5 can be removed from the bottle 1 separately or as one unit.

The culture medium 2 is filled with a gap of about 5 mm from the bottom surface 3b of the inner cap 3, and the diameter of the bottle 1 is about 5 mm.
m, the diameter of the through hole 3C was preferably 15 to 201 m.

Next, a cultivation process using such a cultivation container will be explained.

The bottle 1 is filled with a culture medium 2 mainly composed of ogayo made from broad-leaved trees such as beech and oak, and the inner cap 3 is covered with kraft paper 4 in advance, and the outer cap 5 is fitted over the mouth of the bottle 1. Sterilize using conventional methods.

After cooling, remove the inner cap 3 along with the outer camp 5, quickly plant the seed, and replace the inner cap 3. Cover with outer cap 5.

This work can be done in an extremely short time because the inner cap 3 and outer cap 5 are integrated.

The cultivation chamber is kept at a temperature of 20 to 22°C, a humidity of 55 to 65%, and an illuminance of 50 to 150 lux for 20 days.

Next, increase the indoor temperature to 22-24℃ and the humidity to 55-6℃.
Maintain the concentration at 5% to allow the bacteria to mature. 30-3 from the day of inoculation
When the illuminance is increased to 200 lux after 5 days, germination occurs after about 5 days and fruiting bodies develop over the entire surface of the culture medium.

After fruiting bodies have developed and 14 to 20 days have passed after the end of the culture period, the room temperature is 20 to 23°C, the humidity is 80 to 100%, and the illuminance is 30%.
Keep it at 0 lux.

As a result, the head of the fruiting body is pressed by the bottom surface 3b of the inner cap 3, concentrates in the through hole 3C, spreads into the upper chamber A at once, and grows.

When the fruiting body grows and reaches the lower surface of the outer cap 5, that is, the kraft paper 4, the outer camp 5 and the kraft paper 4 are removed, and the fruiting body is allowed to grow outside the bottle 1.

As a result, it was possible to harvest the bacteria in 55 days after night fishing, the growth period was shortened by about 5 days, and the germination rate was almost 100%, compared to the conventional method that does not use Inner Camp 3. The height of the product was approximately 5cm tall (20-30% increase in sales), and the quality of the product, such as color, was superior to that of conventional products.

In addition, it was confirmed that the □ seeds whose bottom surface 3b of the inner cap 3 was painted black germinated more reliably and grew as fruit bodies.

In addition, the size of the through hole 3C provided in the bottom surface 3b.

The position and number are not limited to those in the above-mentioned embodiments, but any arrangement may be used as long as the two wheels above the surface of the culture medium are communicated and the fruiting bodies are concentrated. Further, the structure of the outer camp 5 may be any suitable structure for holding down the kraft paper 4, and the number of kraft papers 4 may be adjusted depending on the humidity in the room where the cultivation is performed.

In this way, according to the present invention, the inner wall that is fitted into the bottle mouth and pushed into the bottle mouth serves as a partition wall provided above the surface of the culture medium that improves the germination rate, increases the yield, and shortens the growth period. By using a cap, the outer cap is fitted over the inner camp to prevent bacteria from entering, so it is easy to attach and remove the inner camp and outer cap to the cultivation container, and it is easier for bacteria to enter after sterilization. The inner and outer caps can be attached and removed in a short time when inoculating maitake fungi, and the inner cap does not move even when the outer camp is removed and the fruiting bodies grow outside the container. It has great effects such as not damaging the fruiting body.

Although the present invention has been variously explained above with reference to preferred embodiments, the present invention is not limited to these embodiments, and it goes without saying that many modifications can be made without departing from the spirit of the invention. It is.

[Brief explanation of the drawing]

The figures show a preferred embodiment of the present invention, and FIG. 1 is a longitudinal sectional view of a cultivation container and its cap. 1... bottle, la... rib, 2... culture medium, 3
...Inner camp, 4...Craft paper, 4a...
Through hole, 4b...film. 5...Outer cap, 5a...Through hole, A...Upper chamber, B...Lower chamber. Patent applicant Yoshito Kusama (7762) Patent attorney

Claims (1)

[Claims] 1. It is dropped into the bottle mouth of a maitake cultivation bottle, and is located near the culture medium surface without touching the culture medium surface, and has an inverted U-shaped folded part on the upper edge to resist falling and pushing up of the fruiting body. The inner cap is tightly fitted to the bottle opening and has a through hole with a smaller area than the surface of the culture medium on the bottom surface, and the inner cap is fitted onto the folded part of the inner cap to allow the bacteria to enter the bottle opening without entering. A cap for a maitake cultivation bottle consisting of an outer cap that is breathable and translucent.