JPS6022290B2 - blood smear method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a method for preparing a blood specimen for use in a blood specimen observation device and the like.

[Background of the Invention] Blood specimens are smeared one by one onto slide glasses, and stained and observed.

Therefore, in the case of automation, processing of individual specimens is the main reason why the apparatus becomes a complex and precise mechanism. Therefore, instead of smearing blood on a discrete medium called a preparation glass, blood is smeared on a continuous medium called a transparent and flexible film, thereby simplifying the mechanism involved in replacing the specimen. There is a method.

FIG. 1 shows an example of a blood sample preparation method and observation method.

Using an automatic smearing means 1 for smearing blood on a film,
Blood is sequentially smeared onto a certain length of film, the blood on the film is stained using automatic staining means 2, and automatic observation means 3
Observe the sampled blood using Among these means, the automatic staining means 2 and the automatic observation means 3 can be easily realized using known techniques, so only the automatic smearing means 1 will be described in detail below. FIG. 2 shows an overview of the automatic smearing means.

The blood dropping means 4 is used to drop blood in an appropriate shape at an appropriate position on the film. On the other hand, the film is moved in the longitudinal direction by the film feeding means 5. The film on which the blood is applied is drawn together into an appropriate shape by the smearing means 6, and a blood specimen is prepared on the film. In this case, the following methods are available for making inquiries:

Figure 3a shows an example of specimens drawn together in the direction of film movement. This method simply changes the smearing medium from prepared glass to film. A specific method is shown in FIG. 3b. film 7
A wedge 9 made of glass or the like is used to collect the blood 8 dropped onto the
This is a method in which the films 7 are brought into contact with each other and brought together by moving the film 7. In the example described in Tsubaki Kaisho No. 50-139195, blood is dropped and the bong is brought into contact with the smeared surface of the film while advancing in the opposite direction to the film's advancing direction to perform the drawing.

However, in these methods, the degree of blood smear (shade) changes in the direction of film progression. That is, the smear position through observation is perpendicular to the direction of film travel. Therefore, when attempting to observe the required number of blood cells with the automatic observation means, it is necessary to change the direction of the relative position movement of the automatic observation means and the blood sample many times, making the structure of the automatic observation means complicated. [Object of the Invention] An object of the present invention is to provide an excellent blood smear method that can solve the above problems.

[Summary of the invention]

Therefore, in the present invention, blood is dripped linearly in the longitudinal direction of a long film-shaped smear medium, one end is in close contact with the surface of the smear medium film on the blood dripping side, and the other end has a predetermined distance, The alignment is carried out by advancing the smearing medium in the longitudinal direction toward a wedge arranged obliquely with respect to the longitudinal direction.

As a result, the blood that has passed through the gap between the wedge and the film is spread onto the film in a smear thickness distribution that is substantially uniform in the longitudinal direction of the film and has a mirror thickness corresponding to the gap in the width direction. Therefore, the portion of the smeared blood sample that has been observed has a smear thickness distributed at predetermined positions in the width direction of the film in a linear manner along the longitudinal direction of the film.
Therefore, once the position of the automatic observation means in the width direction of the film is adjusted, blood specimens can be observed by simply moving the film sequentially in the longitudinal direction, and the structure of the automatic observation means is extremely simple. [Embodiment of the Invention] FIG. 4b shows a smearing method according to an embodiment of the invention.

In this method, the blood 11 that has been deposited on the film 10 in a linear manner along the longitudinal direction of the film is drawn using a wedge 12 having a slope in the direction of movement of the film and on the film surface. One end of the wedge 12 corresponding to the position at which the blood 11 is dropped is at a predetermined distance from the film surface, and the opposite end is arranged at an oblique angle so as to be in close contact with the film surface. The film 10 onto which the blood has been dropped advances toward the wedge 12 in its longitudinal direction. The blood passing through the gap between the film surface and the wedge is forced and spread on the film 10 according to the gap. Therefore, the state of smearing on the film after passing through the wedge 12 is as shown in FIG. A smear thickness distribution with a large thickness is obtained. The film smeared with blood in this manner is sent to automatic observation means 3 via automatic staining means 2, as shown in FIG.

Here, the observation of the blood specimen is performed continuously at the position of the smear thickness where the blood cells are evenly and somewhat densely distributed. In the smeared state shown in FIG. 4a, the smear thickness portions subjected to such observation are distributed linearly at predetermined positions in the width direction of the film in the longitudinal direction of the film, that is, in the traveling direction of the film. Therefore, if the automatic observation device has a function of adjusting the position of the film in the width direction, it is possible to observe the required number of blood cells by moving the film in the longitudinal direction. Therefore, by using the method of this embodiment, blood smearing, staining, and observation can be performed consistently by continuously moving the film.
[Effects of the Invention] As described above, according to the present invention, blood smearing is performed with a uniform smear thickness distribution in the width direction of the film by the simple operation of moving the film. The effect is that the operation can be carried out extremely efficiently.

[Brief explanation of the drawing]

FIG. 1 explains the premise of the present invention; 1 is an automatic smearing means, 2 is an automatic staining means, and 3 is an automatic observation means. FIG. 2 is a diagram showing an embodiment of the automatic smearing means;
5 is a blood dropping means, 5 is a film feeding means, and 6 is a smearing means. FIG. 3 is a diagram showing an example of a blood smearing method, where -7 is a film, 8 is blood, and 9 is a wedge. FIG. 4 shows a blood smear method according to an embodiment of the present invention, in which 1 is a film, 11 is blood, and 12 is a wedge. Solution, Z diagram, Z diagram, Tsutomoku diagram

Claims (1)

[Claims] 1. Blood is collected onto a long film-like smear medium in a line extending in the longitudinal direction of the smear medium, and the blood is slanted so that one end is in close contact with the surface of the smear medium on the side from which the blood is removed, and the blood is collected in a line extending in the longitudinal direction of the smear medium. The smear medium is advanced in the longitudinal direction toward a wedge disposed obliquely to the wedge, so that the blood passing through the gap between the wedge and the smear medium is substantially uniform in the longitudinal direction of the smear medium; A blood smearing method characterized in that the blood is spread on the smear medium in a width direction with a smear thickness distribution having an inclination according to the gap.