JPS60193468A - Leucocyte removal filter - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a filter for selectively removing white blood cells from a blood cell suspension such as blood or body fluid.

In recent years, due to the development of hematology and immunology, component transfusion has been gaining attention as an alternative to conventional whole blood transfusion, in which only the blood components required by the patient are given, and unnecessary components are not given as much as possible.

Blood component replacement includes red blood cell transfusion, white blood cell transfusion, platelet transfusion,
There are many cases where only red blood cells are transfused to patients with anemia, heart or lung disease, etc., such as plasma transfusion. The reason for this is that patients who receive transfusions of whole blood or concentrated red blood cells experience nausea,
It has been reported that side effects include fever, headache, and nausea. The cause of these is said to be an immune reaction between the HLA antigens or tissue antigens possessed by the white blood cells in the transfused blood and the recipient's antibodies, that is, the production of anti-Japanese blood cell antibodies. In the case of red blood cell transfusion, it is desirable to inject a concentrated red blood cell solution from which antigenic substances such as white blood cells and platelets have been removed as much as possible.

Based on these findings, various methods of obtaining concentrated red blood cells from which all white blood cells and platelets have been removed are currently being investigated.

Broadly speaking, there are 3 types of this method: 9. One method is to separate white blood cells, platelets, and plasma into four parts by centrifugation and obtain concentrated blood. The second method is to add dextran to whole blood, remove the dextran to which white blood cells are attached, and then add physiological The third method is to wash away the dextran with saline to obtain concentrated red blood cells, and the third method is to capture all white blood cells with a filter filled with fibers appropriately to obtain concentrated red blood cells from which leukocytes have been removed.

However, centrifugation requires expensive equipment, requires repeated washing with physiological saline six or more times to remove approximately 90% of white blood cells and platelets, and requires suction of white blood cells. There is a problem in that about 20 cells of red blood cells are wasted because the separated red blood cells are aspirated and separated without removing the cells, but the dextran sedimentation method also requires 6 times to achieve a leukocyte removal rate of about 90%. The above saline washing and dextran washing are necessary and time consuming.
Operability is also difficult.

Japanese Patent Publication No. 5B-54125 and No. 58-54.126 are necessary for obtaining a red blood cell preparation rich in red blood cells without white blood cells by trapping and removing white blood cells using a filter device filled with fibers. , these inventions include synthetic fibers, semi-synthetic fibers, regenerated artificial fibers,
At least one of inorganic fibers and natural fibers is present in the column.
A leukocyte separation filter device packed at a density of 1 sr/7 or less, and a leukocyte separation material made of synthetic fibers, semi-synthetic fibers, recycled artificial fibers, inorganic fibers, or natural fibers that do not denature blood and have an average diameter of 10 μm or less. It is said that leukocytes can be captured and removed from blood with a simple operation in high purity and high yield, but in reality, with a filter of the size used in hospitals, the processing speed is 571 /
It is about 200 mm, and it takes about 40 minutes to process, 5
007 It takes about 100 minutes to process, which is too long as a processing time. Also, if the blood is left at room temperature during this time, the denaturation of the blood will proceed. The capacity of the filter is small, so it is possible to remove fibers of a certain size by 0. i 5 ii'
If it is packed more than /m, it will become clogged because even red blood cells will be captured.
The problem was that the processing speed decreased drastically.

The present inventors have painstakingly investigated a method for separating and removing white blood cells from a large amount of blood in a very short time with a simple rt+i operation, and found that white blood cells with a diameter of 6μ
A nonwoven fabric made of fibers of less than Pressure loss can be reduced, and as a result, processing time can be drastically shortened to about one-tenth, and purity and purity can be reduced.
It has been found that leukocytes can be separated and removed with good yield. In this case, it is preferable that the above-mentioned nonwoven fabric is fixed in position by mutual entanglement of fibers.

According to the present invention, by fully using a nonwoven fabric made of fibers with an extremely small fiber diameter, the filter has high fangs and the fibers are evenly distributed in the vertical and horizontal directions of the filter device. can be filled. Furthermore,
1 Thin fiberfffk can be packed evenly, so it is possible to increase the packing density to an extent that would be unimaginable with thin fibers.

Therefore, the contact area with blood can be increased, and the length in the vertical direction required for removing leukocytes can be significantly shortened.

Therefore, when -1 blood is passed through the filter, the pressure loss in the filter is small, the processing speed is increased, and processing can be performed in a short time.

In addition, by using a nonwoven fabric made of threaded fibers, the fiber gaps are constant and uniform, the fiber 1ffl gap can be reduced, blood channeling can be prevented, and leukocyte removal performance is improved. The unevenness between products will also be reduced. Furthermore, since the product can be made thinner and more compact, the priming volume can be reduced and the recovery rate of red blood cells can be improved. In addition, the high processing speed and short operation time can prevent any rise in blood temperature when blood stored at 4C is processed at room temperature, and is also effective against blood denaturation. Furthermore, by using a non-woven fabric, it is possible to eliminate lint produced during treatment and prevent lint from being mixed in during blood transfusion.

The fibers used in the present invention are synthetic fibers that do not cause total blood denaturation, and include polyamide, aromatic polyamide, polyester, polyacrylonitrile, polytrifluorochloroethylene, polymethylmequaacrylate, polystyrene, polyethylene polypropylene, and the like. To make all the fibers with a diameter of 6 μm or less, generally 9,
The method for producing IL fibers with a diameter of 3 μm or less is the melt-blowing method.
cess), and all fibers of the present invention having a diameter of 5 μm or less were prepared by this method. However, the fibers of 6 μm or less used in the present invention are not limited to this method.

It is preferable that the non-woven fabric of "KIENΦ" is one in which the fibers are fixed in such a way that they are entangled with each other, and the method of fixing the fibers to make such a non-woven fabric is as follows: Of course, it is necessary to apply heat close to the melting point of the fiber or to cure it by heat, or to fix it with an adhesive.
Although it can be used in the present invention, when the fibers are thin like the fibers used in the present invention, the fibers can be fixed by simply entangling the fibers under total pressure using an air blow method, high pressure steam blow method, etc. The created nonwoven fabric is, for example, (l
! ! : A huge force that could destroy it? It is stable unless added, and can withstand blood treatment well. therefore,
In this way, it is also possible to adopt a method of fixing the fibers by simply entangling the fibers with each other, such as an air blowing method or a high-pressure steam blowing method, which does not cause the fibers to be heated too quickly.

The diameter of the fibers constituting the entire nonwoven fabric of the present invention is less than 3 μm, preferably from 0.1 μm to less than 3 μm, and more preferably from 0.1 μm to less than 3 μm.
Preferably, it is in the range of 0.1 μm to 2.0 μm. If the diameter of the fibers is smaller than 0.1 μm, it is actually difficult to make, but it tends to be bulky.If the fibers are packed evenly, the gaps between the fibers will be extremely small, and red blood cells will be captured along with white blood cells. This results in clogging and extremely reduced processing speed. If the diameter of the fibers is 3 μm or more, it is necessary to increase the packing density and reduce the fiber gaps.
For example, for fibers with a diameter of 3 μm or more, a, so y
Unless the packing density exceeds 1 crd, the leukocyte removal rate will decrease. Furthermore, since the packing density is high, the packing must be carried out under high pressure, which will prevent the fibers from breaking. However, because the contact with blood in a given volume is small, the valley shape of the leukocyte removal filter becomes extremely sharp, making it difficult to handle in hospitals.

The density of the nonwoven fabric of the present invention is o, 1s7/7 and 0.5
It should be in the range of 0 f /cd, preferably 0.20 f /crd to Ll, 60 p /d
is within the range of The density of the nonwoven fabric is o, is! If it is smaller than i'/7, for example, when treated for a short time of 50 mel-, white blood cells will increase. , ”; ;/1. When the density of the nonwoven fabric becomes larger than 0.50j//, the cutting,
As the gaps become denser and red blood cells are also trapped, the recovery rate of red blood cells decreases.

The thickness of the nonwoven fabric of the present invention ranges from 111i11 to 301fl for short-time treatment of blood and blood cell suspensions! l, preferably 1111111 to 20 b+lll %, more preferably 2141111 to 1 o rr+rn o
range. When the thickness of the nonwoven fabric becomes thinner than I Mm,
The capture of leukocytes is not sufficient, and the capture rate of leukocytes decreases. On the other hand, if the thickness of the nonwoven fabric is greater than 50 mrn, the pressure loss will increase and the processing speed will decrease.

In order to process blood and blood cell suspension in a short period of time, the filter area of the present invention is determined by the blood population 1 including the gap between fibers 1 and 1.
The rule is that the bubble area is 1ocrtl/blood relatives 500 to 200
0Cd/blood7ys o o)nl, preferably 20(
Go/Blood so o me to 1ooOd/Blood 7f 5
00rnl.

More preferably 3D blood/500ml to 300d
/Blood 500-. Filter area is 10c
When it is smaller than rl/blood so o me, the surface of the leukocyte removal filter becomes saturated with leukocytes, resulting in a slow processing speed. Also, the filter area is 200
OC11/If the blood size is larger than 500 ml, it is necessary to use a lot of menstrual water to collect red blood cells in the leukocyte removal filter after leukocyte removal treatment, and red blood cells remaining in the leukocyte removal filter. Since the number of red blood cells increases, the recovery rate of red blood cells decreases.

Note that the bulk density of the nonwoven fabric of the present invention refers to the uniform nonwoven fabric 17
The value of that heavy 6111 hiki is completely revised.

It is possible to obtain a high yield with a simple operation from a large amount of blood, which is the object of the present invention. The density is 0.157/d7. ．． 11. -0.50 f 1Cu
NJ (lower part) A filter consisting of unscrupulous F is necessary, preferably a non-woven fabric fixed in position by the mutual entanglement of the fibers; Density, thickness, and 0. The filter quadriproduct is within the above range.

Hereinafter, details of the leukocyte removal filter of the present invention and a filter device using the filter will be explained with reference to the drawings.

1 and 2 show one embodiment of a filter device using a leukocyte removal filter of the present invention, and FIGS. 3 and 4 show another implementation. In FIGS. 1 and 2, 1 is a filter device main body, and two round tray-shaped frames 2 and 2' are fitted together with a ring member 6 so that the entire desired body is formed. A large number of protrusions 4, 4' are formed on the inner surfaces of the protrusions 2, 2', respectively, and a filter 6 made of a non-woven fabric is provided inside the protrusions 4, 4' sandwiched between mesh-like supports 5, 5'. . 7 is one round tray-shaped frame body 2
8 is a blood outflow pipe provided in the other round tray-shaped frame 2', and 9.9' is a backing. The filter 6 is made up of mesoche-like support materials 5, 5.
', the density exceeds Jl 5 f,/i by 0.50
It is maintained within a range of 2/d or less.

The filter devices shown in FIGS. 6 and 4 are similar to the filter device described above, but a blood inflow pipe 16 and a blood outflow pipe 14 are provided at the center of the round tray-shaped frames 10 and 10', respectively. In the cavity, a mesh-like support material 11,
A filter 12 made of non-woven fabric is provided between 11'. i 5 , 15' is banking.

The mesh-like support material is shaped like a mesh so that the blood fluid flows uniformly, and also serves as a flow path regulating section.

The blood is introduced through the blood inflow tube 7, 16, and passes through the mesh-like support 5, 11, resulting in a uniform flow.The blood is then introduced into the filter 6 or 12, where white blood cells, platelets, etc. The collected red blood cells and plasma pass through the dish, which becomes leukocyte-removed concentrated red blood cells, and the mesh-like support member 5' and b 11' pass through the entire dish, resulting in a more uniform flow. Or it will be collected from 14th.

The above filter selectively captures white blood cells, platelets, etc. in blood due to the adhesiveness, chargeability, hydrophobicity, and fiber gaps of the fiber surface, and allows only red blood cells and plasma to pass through. The effects of , chargeability, and hydrophobicity are greatly influenced by the fiber gap. 1. The above filter is made of fibers with a diameter of less than 5 ltm, and the shape of the nonwoven fabric is cotton-like, cloth-like, or IR fiber-like. The non-woven fabric 15 is a non-woven fabric in which the fibers are circularly formed by thermal welding or the like, so that the gaps between the fibers are fixed and can be made uniform. The filter device 11 is a simple non-woven filter with a diameter of less than 5 μm [Compared to a full-scale leukocyte removal filter device, it is more effective in preventing blood chorion, improving leukocyte and platelet removal performance, reducing operation time, and It is effective in preventing dirt such as lint from getting mixed in.

Furthermore, the ability to capture white blood cells, platelets, etc. and the permeability of red blood cells and plasma are dependent on the bulk density of the nonwoven fabric.

Healthy human whole blood, P RCu, and bovine whole blood, P RC
Based on the results of the exam for the detailed bar using the money, it will be better if the results are good.
The bulk density of is greater than 0.159/Cd and 50y/c
m or less is suitable, high density I of nonwoven fabric made of polyester thread
Is f 0.30? /c+cl presentation degree is preferred.

rAE" The figure shows one mode of use of the filter device using the leukocyte removal filter of the present invention.

The blood collected from the human body is supplied from the blood collection bag 16 by the differential pressure to the filter device 18 using a leukocyte removal filter of the present invention.The blood introduced into the filter device 18 In this case, white blood cells, platelets, etc. are captured by the white blood cell removal filter made of non-woven fabric of the filter device, and the blood mainly consists of red blood cells and plasma.After passing through the circuit 19, it is sent to the collection bag 20.Recovery of the red blood cells. 4. If you want to increase the concentration more than 4, introduce the saline bag 21 prepared in advance into the filter device 18 through the entire circuit 22, and add -tXV, remaining red blood cells 7, and more to the circuit and leukocyte removal filter. In addition, 23 is a collection bag, and 24 is an adjustment valve.

The above example shows blood collected from a human body contained in a blood collection bag. It is also possible to return blood, mainly plasma, back into the body.

As described above, the leukocyte removal filter according to the present invention can separate and remove leukocytes from blood or a blood cell suspension in a very short period of time with simple operations and a low yield. be.

Examples will be described below.

Example 1 Polyester fibers with a diameter of 1.2 μm A non-woven metal fabric with a bulk density Jl 8 f/cn′l fabricated by the total melt blowing method and fixed in position by mutual entanglement of the fibers, diameter 110111111 ．． Cut into a cylindrical shape with a thickness of 7 Tnm, an effective inner diameter of 1001111a (effective inner diameter = diameter of the part where blood or blood cell suspension actually contacts the filter surface. The same applies to subsequent examples), and a thickness of 11 mm. was fixed in a column. 600- blood collection bag 2
Attach the above leukocyte removal filter device at a height of 800 + 11111 from each individual, and add the aoomm
Attach a 1 ton infusion bag to the T side for processing [and a collection bag for storing t L, and a bag with an inner diameter of 5111 mm between each.
A. A processing device T connected with tubes having an outer diameter of 5 mm was prepared. Two blood collection bags of this processing device contain 25 liters of fresh CPD solution-added whole blood with a hemadcrit of 38% from a healthy person with type A blood.
0 ml each was added, and leukocyte removal treatment was performed one bag at a time at a room temperature of 25C using a gravity drop method using a drop.

Next, 90 ml of physiological saline was poured into this leukocyte removal filter device by a gravity drop method, and the red blood cells in the filter were collected.

As a result, the processing time for this fresh blood 500- is 6 minutes 35 minutes.
It was a high flow rate of 76-7 minutes in terms of processing speed. Also, what is the leukocyte removal rate? The red blood cell recovery rate was 9.7%, and the red blood cell recovery rate was 95%.

Comparative Example 1 Diameter is 9.6 μm, length is 40 l1ju to 7011
1111 polyester fiber with a diameter of 5011111
1. Leukocyte removal filter device (bulk density o, 1s y
/crd) was created. Attach the leukocyte removal filter device described above at a distance of a o o mm from each of the two 300 g blood collection bags, and then add the 80011 III blood collection bag.
+ A 1 ton infusion bag is attached below, and a collection bag for storing the processed blood is placed between them, each with an inner diameter of 3111 mm.
15, a complete processing apparatus connected with tubes having an outer diameter of 514 mm was prepared. Into two blood collection bags of this processing device, put 250 g of fresh CPD solution-added whole blood of 38 hematocrits of a healthy person with blood type A, and collect one bag at room temperature of 25 U using the natural fall method using the drop. Leukocyte removal treatment was performed on each sample in turn.

Next, 80 g of physiological saline was poured into this leukocyte removal filter device by a gravity drop method, and the red blood cells in the filter were collected.

As a result, this fresh blood amounted to 500m7! The processing time was 1 hour, 52 minutes, and 35 seconds, and the processing speed was 5.4-7 minutes. Furthermore, the leukocyte removal rate was 93.2 and the red blood cell recovery rate was 96.

According to Example 1, the leukocyte removal filter of the present invention includes:
Compared to conventional leukocyte removal filters, the processing speed was about 14 times faster, the leukocyte removal rate was good, and the red blood cell recovery rate was excellent, comparable to the conventional method.

Example 2 Polyester fibers with a diameter of 1.8 μm were made by melt blowing and had a bulk density of 0.22? A nonwoven fabric in which the fiber mass of /art was fixed in its position by the mutual entanglement of the fibers was cut into a columnar shape with a diameter of 78 mm and a thickness of 411 + 1 nm, and was fixed in a cylindrical shape with an effective inner diameter of 6 am + j and a thickness of 8 m + a. . The above-mentioned leukocyte removal filter device was attached to a position with a head height of 800 mTh from two 500 me blood collection bags, and a 1 ton RL fluid bag was attached below 8001 nn to serve as a collection bag for storing the processed fluid. The inner diameter is 311111 and the outer diameter is 511, respectively.
A processing device connected with 111 tubes was created. Into two blood collection bags of this processing device, 42 grams of fresh ACD-A fluid-added blood, oil-added whole blood, and 0 me of whole blood from a healthy human being of Bq were added, and the blood was collected by gravity using the full head at room temperature. Leukocyte removal treatment was performed one bag at a time at 25 tl'.

Next, 40 me of physiological saline was poured into the leukocyte removal filter device using the natural submergence method, and the red blood cells in the filter were collected.

As a result, the processing time for this fresh blood 500- is 7 minutes 15 minutes.
It was a high flow rate of 69 ml and 11 minutes in terms of processing speed. In addition, the leukocyte removal rate was 100% and the red blood cell recovery rate was 98%.

Comparative Example 2 Diameter is 7.2 μm, length is 40 rtnn to 7011
1E nobolyamide fiber (nylon-66) f, diameter 6
Leukocyte removal filter device (bulk density 0.09
3/7) was created. Attach the above leukocyte removal filters at a height of 800 mra from two 300 rd blood collection bags, and then add 800 +, 'Irn.
A 1 ton infusion bag is attached at the bottom to serve as a collection bag for storing all the processed blood, and each bag has an inner diameter of 3111 J.
A processing device was created that was connected by tubes with an outer diameter of 5 mm. Two blood collection bags of this processing device contain 250 whole blood of a healthy person with blood type B, Hemadcrit 42, fresh ACD-A liquid-added blood.
ml each, and by the natural fall method using the drop,
Leukocyte removal treatment was performed one bag at a time in room @25C.

Next, add 80rn1 of physiological saline. -i By the gravity fall method, the red blood cells were poured into this leukocyte removal filter device and the red blood cells in the filter were collected.

As a result, the processing time for this fresh blood 500- is 1 hour 2
The processing time was 4 minutes and 45 seconds, and the processing speed was 5°9-7 minutes. Furthermore, the leukocyte removal rate was 95.1%, and the red blood cell recovery rate was 94%.

According to Example 2, the leukocyte removal filter of the present invention includes:
Compared to conventional leukocyte removal filters, the processing speed was about 12 times faster, the leukocyte removal rate was good, and the red blood cell recovery rate was also excellent, comparable to the conventional method.

Example 6 A nonwoven fabric with a diameter of 100111111, in which a high-density J169/7 fiber mass made of polyamide fibers (nylon-66) with a diameter of 0.8 μm by a melt-blowing method was fixed in position by the mutual entanglement of the fibers. It was cut into a cylindrical shape with a thickness of 2 Tnm and fixed in a column with an effective inner diameter of 90 mm and a thickness of 6 TnrA. Attach the above-mentioned leukocyte removal filter device to the blood sampling tube of 600-1 (at a height of 8001111fl from the knob 21), and then
A 1 ton infusion bag is attached to the lower part of the bag, which serves as a collection bag for storing the treated liquid.
A processing device was created which was connected with tubes having an outer diameter of 5 + 11111 mm. Put 250 ml of fresh whole blood of a healthy person with blood type A (hematocrit) 4591 into two blood collection bags of this processing device, and collect one bag at a room temperature of 25C using the gravity drop method using a drop. Leukocyte removal treatment was performed on each sample in turn.

Next, physiological saline was poured into this leukocyte removal filter device by a 50-degree gravity drop method, and all the red blood cells in the filter were recovered.

As a result, the processing time for 500 ml of fresh blood was 6 minutes and 29 seconds, which was a processing speed of 77 and a high flow rate of 1 minute per ml. Furthermore, the leukocyte removal rate was 99.9, and the red blood cell recovery rate was 98%.

Comparative Example 3 Leukocyte removal filter device (bulky Density 0.0901/crd)
It was created. Attach the leukocyte removal filter described above at a height of 800 mm from two 300 rnl blood collection bags, and attach a 1 ton infusion bag to the lower part of the tube to serve as a collection bag for storing the processed blood. A treatment device was prepared in which each tube was connected with a tube having an inner diameter of 5 mm and an outer diameter of s mm. Fill two blood collection bags of this processing device with 250 me of fresh CPDl-added whole blood of 45 hemadrits of type A healthy people, and collect leukocytes one bag at a time in chamber 125C using the natural fall method using a drop. A removal process was performed.

Next, add 210 ml of physiological saline using the gravity drop method.
The blood was passed through this leukocyte removal filter device, and the red blood cells inside the filter were collected.

As a result, the processing time for 500 liters of fresh blood was 19 minutes.
The processing time was 4 seconds, and the processing speed was 2'6 ml in 7 minutes. However, the leukocyte removal rate was 68.4% and the red blood cell recovery rate was 91.3%.

According to Example 6, the leukocyte removal filter of the present invention has 7
It was a fast process of 8-7 minutes. I also tried high-speed processing using the conventional method, but the processing speed was only 26m7! When the speed was increased to /min, the leukocyte removal rate decreased. Therefore, in the conventional method, there is a limit to how high the processing speed can be increased, and high-speed processing as in the present invention cannot be achieved.

Example 4 Polyester 111 fibers with a diameter of 2.8 μm were made by the bath melt blowing method.The bulkiness was 0.28? When producing the nonwoven fabric No. 7, a dry heat treatment was performed at 250 C for 2 seconds during the melt blowing process to create a nonwoven fabric in which the fibers were tied together and the contact points were thermally fixed. This non-woven fabric has a diameter of 160 m3 and a thickness of a nun.
Cut into a cylindrical shape with an effective inner diameter of 150,711 m.

It was fixed in a column of 12 Il+rn. 300
- Attach all of the leukocyte removal filters mentioned above to the two blood collection bags at a height of 800 m, respectively.
A 1 ton infusion bag is attached below m to serve as a collection bag for storing the treated solution, and the inner diameter of the bag between them is 31111 mm.
11. A processing device connected with tubes having an outer diameter of 51+1I11 was created. The blood collection bag 21 of this processing device has 0
Concentrated blood containing CPD solution (from which a portion of the plasma has been removed by centrifugation) of a healthy human body type was stored at 4C for 200 days per day, and then allowed to fall naturally using a drop. According to the method, each bag was taken out from a 4C refrigerator and immediately subjected to leukocyte removal treatment in order at a room temperature of 10C.

Next, add 220 ml of physiological saline using the gravity drop method.
It was passed through this leukocyte removal filter, and the red blood cells inside the filter were collected.

As a result, the processing time for 400 units of fresh surface was 6 minutes and 27 seconds, and the processing speed was as high as 62me1 minute. -! The leukocyte removal rate was 98.9, and the red blood cell recovery rate was 91%.

[Brief explanation of the drawing]

FIG. 1 is a front view showing one embodiment of a filter device using a leukocyte removal filter of the present invention, FIG. 2 is a vertical side view of the same, and FIG. 3 is a front view of another embodiment. The vertical side view, FIG. 5, shows how the filter device using the leukocyte removal filter of the present invention is used. FIG. 1... Filter device body 2, 2'...
... Round tray-shaped frame body 3 ... Ring member 4, 4'.
... Protrusions 5, 5' ... Mesh-like support material 6 ... Filter 7 ... Blood inflow pipe 8 ... Blood outflow pipe 9 ,9'...
...Kutsuki rug 10, 10'...Round tray-shaped frame 11, 11'...Mesh-shaped support material 12
... Filter 16 ... Blood inflow pipe 14 ... Blood sample M output 1i7 15 , 15'.
・・・・・・Nokutsukirag 16・・・・Blood collection bag
17...Circuit 18...Fill-coup device 19...Circuit 20...Collection bag 21...Physiological saline bag 22...
...Circuit 26...Recovery (lag 24...
...Adjustment valve 1st circle 2nd figure ↓ -l Figure 3 0 40 Figure 5

Claims (2)

[Claims] (1) The fiber diameter is less than 3μm and the bulk density is 0.15f
A leukocyte removal filter made of a non-woven fabric with a total exceedance of /crA of 0.50 f/cd or less. (2) The leukocyte removal filter according to claim 1, wherein the nonwoven fabric is fixed in position by mutual tangles of fibers.