JPS60188845A - Particle analyzing device - Google Patents

Abstract

PURPOSE:To detect specific cells with good accuracy by dispensing a constant amt. of the blood in which a phosphorescence generating material is incorporated, diluting the same by an electrolyte in the constant amt., supplying the liquid to an optical cell, supplying the electrolyte under specified pressure from the side part and irradiating a luminous flux of a specified width thereon then detecting phosphorescence. CONSTITUTION:A blood 13 added with a reagent to be labeled with phosphorescence is fed to a blood detector 14 from a sampling nozzle 11 by a squeezing pump 3 and after detection, the pump 3 is stopped and a specified amt. is dispensed by a metering valve 16 of a selector valve 1. The dispensed blood 13 is discharged together with an electrolyte 10 through a flow passage 18 to a liquid container 19 by a cylinder pump 5 and is stirred by the compressed air from a compressor 21. A specified amt. of the blood dispensed through a valve 31, a selector valve 2 and a metering valve 23 is connected to a flow passage 25 and an electrolyte 28 is pressured. The blood is forced into a photocell 26 after specified time. The blood flows through the inside of the cell 26 while the blood is finely restricted by the sleeve of the liquid 28. The blood is subjected to photometry by a detector 12 during the passage through the inside of the luminous flux from a light source 20, by which the kind of the cells is analyzed from the antigen-antibody reaction.

Description

[Detailed Description of the Invention] [Field of Application of the Invention] The present invention relates to the type, number, and thickness of particles suspended in a solution.
- It relates to a particle analyzer for classifying particles, and more particularly to a particle analyzer for continuously analyzing particles in a solution flowing continuously at a predetermined flow rate.

[Background of the invention]

This specification relates to a particle analyzer suspended in a solution, but to make the explanation easier to understand, an example will be given of cell analysis of blood cells in the bloodstream.

Conventional sleep analyzers detect antigens in the blood by containing a fluorescent substance that emits fluorescence when the subject (blood) is exposed to light and extracting antibodies from the blood to cause an antigen-antibody reaction. There is. For this detection, conventionally, when blood is made into a thin flow and the flow is shined with light, the cells in the blood are exposed to the light that crosses the light, and the fluorescently labeled cells emit fluorescence 71+-. Cells floating in blood are analyzed by detecting fluorescence with a detector. However, in conventional devices like this, cells are analyzed by detecting fluorescence, so in many cases the cells themselves emit fluorescence, and when this self-emitted fluorescence is detected as fluorescence from an antibody reaction. This method has the disadvantage of lowering sensitivity and poor analysis accuracy due to interference (noise).

[Purpose of the invention]

An object of the present invention is to provide a particle analyzer that can accurately detect specific cells.

[Summary of the invention]

The present invention aims to detect all the cells of Tokuya with high accuracy by incorporating a phosphorescent substance into an antibody that uses a fluorescent substance and causing an antigen-antibody reaction to analyze the cells. It is something.

[Embodiments of the invention]

Examples of the present invention will be described below.

FIG. 1 shows a flow path system diagram to which a particle analyzer according to the present invention is applied.

In the figure, one input end of the switching valve 1 has a solute liquid 10
is configured to be inhaled by the syringe pump 5. Further, a sampling nozzle 11 is fitted to the other input end of the switching valve 1, and is configured such that blood t13 can be supplied from the tip of the sampling nozzle 11.

This switching valve 1 has a configuration of a metering valve 16 that switches a flow path 15. A flexible tube 4 is connected to one output end of the switching valve 1. This soft tube 4 is provided with a detector 14 and a squeeze pump 3.

Further, a flow path 18 is connected to the other output end of the switching valve 1, and the tip of this L path 18 is connected to a liquid container 19. The liquid in the liquid container 19 is configured to be agitated by air supplied from the compressor 21 via the valve 30. In addition, this liquid container 1
One input end of the switching valve 2 is connected to the output end of the switching valve 9 via the valve 31. At the other input end of this switching valve 2,
A valve 34 is connected via the flow path 24 . This valve 34
At the output end of ``Turtle electrolyte liquid 10'' is applied by Shirinshibo/Pro.
It is composed of vomiting that can be inhaled. In addition, the input end of the valve 34 has i4! , a flow path through which the substance fluid 28 can be inhaled is connected.

Further, the switching valve 2 is configured such that the flow path 22 is switched by a short/open valve 23, and a syringe pump 8 is connected to one output end of the switching valve 2. Further, an optical cell 26 is connected to the other output end of the switching valve 2 via a flow path 25. A valve 35 is connected to the other input end of the optical cell 26 via a flow path 29. This valve 3
5 is composed of sea urchins. Further, a flow path is connected to the other terminal of the valve 35 so that the electromagnetic liquid 28 contained in the tank 27 can be sucked. Furthermore, a syringe pump 8 is connected to the output end of the optical cell 26 via a flow path in which a valve 32 is provided.

This optical cell 26 is used for detecting the light emitted by the light source 20 with fluorescence added thereto by the detector 12.

Since it is constructed in this way, the switching valve 1 is
Unfortunately, as shown in Figure 3, it has the function of cutting a part of the flow path by rotating or moving it up and down, separating a part of the liquid, and switching it to another flow path. The switching valve 2 also has this function. Further, the squeeze pump 3 is squeezed by a roller 4t of a soft tube, and the liquid in the tube 4 is transferred in the same direction as the rotational direction of the roller. In addition, the syringe pump 5°6.7.8 is fully equipped with a syringe pump 5°6.7.8 that sucks up the diluent lO and sends a constant amount of liquid to the flow path according to a command.

The optical cell 26 is referred to as a sheath flow cell, and as shown in FIG. Send it in. At this time, the blood forms a narrow stream wrapped in a sheath of the electrolytic negative solution supplied from the liquid inlet 37. The thickness of this blood flow is determined by the ratio of the flow rate of the MLs negative liquid to the flow rate of the blood, and can be freely selected depending on the necessity. Currently, blood is collected from the patient and tested.
y, - When receiving, add a reagent to phosphorescently label cells in the blood, shake well to stir, and then apply the V/pulling nozzle 1.
Setting it to 1 makes me vomit. When this blood is set, the dIIJ regular vessel opening start switch (not shown) is activated. Then,
First, the squeeze pump 3 rotates, the tube 4 is squeezed, and the blood 13 is sucked through the sampling nozzle 11, and is sucked by the blood detector 14''!.

When this blood is detected, the detector 14 is activated and the squeeze bomb 3 is stopped. On the other hand, a certain amount of blood 13 is dispersed in the flow path 15 of the switching valve 1 by switching the metering valve 16 due to the above operation. A fixed amount of blood 13 is taken out by the metering valve 16, and is discharged into the liquid container 19 through the channel 18 by the operation of the syringe pump 5, together with the electrolyte solution 10 metered into the channel 17. . Spiked blood 1
3 and the electrolyte solution 10 are stirred by compressed air from the compressor 21 when the valve 30 is opened. Next, when the valve 31 is opened, the blood diluted with the electrolyte solution is sent to the switching valve 2. When the flow path 22 of this switching valve 2 is filled with blood, the metering valve 23 is operated and the diluted blood collected in a certain amount in the flow path 22 is transferred to the flow path 24.
25.

Then, the valve 33 is opened, the FkL solute gzs in the tank 27 is pressurized with the compressed air of the compressor 21, and the flow path 2 is
4. Pressurize the channel 29. After the internal pressures in the channels 24 and 29 have increased for a certain period of time, the valves 34 and 35 are switched, and at the same time, the valve 32 is opened and the syringe pump 6.7 is driven. The syringe pump 7 slowly pushes the electrolyte solution 28 into the optical cell 26 through the solution injection port 37 shown in FIG. On the other hand, at the same time, the diluted blood was pushed in from the sample injection port 36 shown in FIG. flows to On the other hand, the light from the light source 2o is constantly irradiated in a direction perpendicular to the optical cell 26 and at the center of the flow of diluted blood narrowly squeezed into the sheath. In this way, even if the light beam 40 emitted from the light source 20 hits the flow of diluted blood 41 as shown in FIG. 5, no phosphorescence is generated when no blood cells are present. In contrast, cells floating in diluted blood 41 (
When the blood cells (blood cells) 42 pass through the light beam 4o, they receive light and generate a phosphorescent sound. This phosphorescence is measured by a detector 12 and the type of cell is analyzed from the antigen-antibody reaction.

Analysis of cell types by measuring with this Rinkosha Gendometer will be explained using FIGS. 4 and 5.

After the light beam 40 from the light source 20 passes through the optical cell 26, the blocker 3B blocks the light from being scattered elsewhere. Further, the detectors 12 are arranged at nine points along the optical axis of the light source 2o, so that they do not sense the light from the light source 20 and can detect phosphorescence with high sensitivity. The blood 41 to which a labeling reagent has now been added to the cells in the blood from the sample injection port 36 is tightly tied with the electrolytic solution from the liquid injection port 37 and transferred to the optical cell 26.
Pour into. In the middle of the flow, the cell 42 shown in FIG.
When it hits 0, the cells emit fluorescence and phosphorescence. However, if a time difference is provided between the fluorescence or phosphorescence generation point 46 and the measurement point 45 of the detector 12, the fluorescence 44 disappears and only the phosphorescence 43 can be detected. As described above, labeled pressure cells in blood can be detected and analyzed with high accuracy. Figure 6 shows a control block diagram of the particle analyzer according to this embodiment.

When the laboratory technician sets the blood 13 containing the labeling material in the sample suction nozzle and presses the start switch 56, the computer 5
0 issues an instruction to the camphor control unit 51 to suck up the blood 13, and controls the switching valve 1, squeeze pump 3, and syringe pump 6 to sample, dilute, and stir the blood 13.
A sample is introduced into the optical cell 26, cells in the blood are analyzed, and the results are printed on the printer 53 and the data are displayed on the C-T54. Furthermore, the computer 50it monitors each part, displays the failure location on the CRT 54, and performs operations such as issuing an alarm to the user with a buzzer.

Therefore, according to this embodiment, a phosphorescent label is used for cells in blood, and the position fR of the light irradiation axis to the cells and the phosphorescent detector is
By shifting by k, stable cell detection can be performed.

〔Effect of the invention〕

As explained above, according to the present invention, specific cells can be
It can be detected anonymously.

[Brief explanation of the drawing]

Figure 1 is a flow path system diagram to which the present invention is applied, Figures 2 and 3.
The figures are a diagram showing the switching between blood constant and preparative separation using the switching valve shown in Figure 1, Figure 4 is a detailed view of the optical cell shown in Figure 1, and Figure 5 is a diagram showing the fluorescence and FIG. 6 is a control block diagram of the apparatus shown in FIG. 1. 10.28... Electrolyte solution, 12... Detector, 13.
... Blood, 16.23 ... Metering valve, 2o ... Light source,
26...Optical cell. Agent Patent Attorney Tatsuyuki Unuma also Figure 2 Sora 3 Figure 1) Quasi-t4-Figure '36 Rate 5 Figure

Claims (1)

[Claims] 1. Quantitative fractionation of blood gold containing phosphorescent substance Kanakura
a second means for diluting the blood separated by the first means with an electrolyte solution; and a third means for supplying the blood diluted in the second means to the optical cell. , a fourth means for supplying the stain solute at a certain pressure from the side of the optical cell supplied in the third means;
A fifth means for irradiating the optical cell with a light beam of a constant width from a light source, and a sixth means for detecting phosphorescence of diluted blood after being irradiated with the light beam by the fifth means. Particle analyzer.