JPS60168346A - Feed for breeding female pig - Google Patents

Abstract

PURPOSE:A feed for breeding female pigs, obtained by incorporating bacteria Bacillus licheniformis therein, capable of improving the survival rate of borne piglings, and having high safety. CONSTITUTION:A feed for breeding female pigs containing bacteria Bacillus licheniformis. The bacteria Bacillus licheniformis to be used are facultative anaerobic bacteria belonging to the genus Bacillus, and not only bacteria isolated from nature but also a variant strain thereof may be effectively used. A variant strain Bacillus licheniformis MN-001 deposited as FERM-P No.266 MN- 001 is particularly preferred for the strain. The bacterial Bacillus licheniformis are cultivated in a liquid or solid culture medium. The amount of the bacteria Bacillus licheniformis to be added is 10<2>-10<15> bacterial cells, most desirably 10<5>-10<10> bacterial cells per kg feed.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a feed for breeding sows that improves the survival rate of newborn piglets, which is characterized by containing Bacillus licheniformis bacteria.

Normally, each breeding sow gives birth to around 10 piglets. However, not all piglets born are weaned. Piglets are born weak and die before they are weaned. The number is by no means small.

In other words, the current survival rate (number of piglets weaned/number of piglets born) is unsatisfactory, and is a major issue that needs to be improved for breeders aiming for efficient livestock management.

Conventionally, in order to solve this problem, extensive research has been conducted in the aspect of animal breeding.

The present inventors have conducted extensive research with the aim of improving the survival rate without relying on such breeding. Various studies have been carried out to improve the survival rate of piglets by incorporating microorganisms isolated from nature into feed for breeding sows. As a result, it was completely unexpectedly discovered that a feed for breeding sows containing Bacillus licheniformis is not biotoxic and has an excellent effect of improving the survival rate of piglets, leading to the completion of the present invention. It is something.

Next, the present invention will be explained in detail.

The Bacillus licheniformis bacteria used in the present invention are facultative anaerobic bacteria belonging to the genus Bacillus, and most of them are isolated from nature, and mutant strains thereof can also be used effectively. Among them, a preferred strain is MN-
This is the mutant strain Bacillus licheniformis MN-001 deposited as 001.

The mycological properties of this Bacillus licheniformis strain MN-1101 (hereinafter referred to as MN-001 strain) are shown.

A, form (1) Bacillus with a width of 0.5 to 0.8 μm and a length of 2 to 3 μm.

(2) Durham staining: positive.

(6) Forms oval endospores.

(4) Motility: 1.

B. Growth (1) Ordinary agar medium: Very good growth. No pigment formation.

(2) Medium mainly composed of molasses and ammonium sulfate: Grows very well. No pigment formation.

(6) Salt liquid medium 5chi: Grow.

7chi: Grow.

10chi: Grow.

C9 Physiological properties (1) Catalase production - (2) Growth under anaerobic conditions (3) VP reaction - (4) Growth conditions at high temperatures 50°C + 55°C - (5) Acid production from sugars Presence of production Glucose + L-arabinose + 1)-gyzolose + mannitol + D-tagatose - (6) Arginine dihydrolase - (kalecithinase) - (8) Hydrolyzability of starch + (9) Hydrolysis of gelatin (1(])T') Hydrolyzability of NA + (11) Utilization of citrate + (12) Utilization of propionate 10(13) Nitrate reducibility 10(6) (14) Indole Production (15) Deamination of enylalanine Bacillus licheniformis, which is an effective bacterium of the present invention, can be cultured by liquid culture or solid culture according to a conventional fermentation method.

As the medium, natural medium, semi-synthetic medium, synthetic medium, etc. can all be used. The composition of the medium is as follows: (i) carbon sources include glucose, sucrose, dextrin, glycerin, starch, molasses, etc.; (++) nutrient sources include peptone, meat extract, yeast extract, dried yeast, soybean meal, (iii) Inorganic salts include phosphates, nitrates, carbonates, chlorides of magnesium, manganese, potassium, calcium, iron, etc. used.

Furthermore, amino acids, vitamins, nucleic acids, and related compounds thereof may be added. The culture temperature is based on the usual fermentation method, but the culture can be suitably carried out at, for example, 20°C'-50°C.

The culture period is also similar to normal fermentation method (4) However, it can be suitably cultured for, for example, 12 hours to 5 days.

The culture thus obtained can be used by repeatedly shattering or washing it to make only the bacterial cells.
The culture, its treated product, and the bacterial cells can be used as they are or by adding all additives and forming a formulation by freeze-drying, spray-drying, or the like.

Furthermore, for example, the culture solution can be strained and formulated using appropriate diluents such as wheat flour, starch, dextrose, etc., grains used as feed raw materials, bran such as defatted rice bran, oil cake, etc. is also possible.

Alternatively, only the bacterial cells may be washed and separated from the culture solution, formulated in the same manner, and used.

The feed for breeding sows of the present invention is characterized by containing Bacillus licheniformis, and although the method for producing the feed for breeding sows is not limited, it is usually a Bacillus licheniformis preparation, only bacterial cells, or It is easily produced by mixing the culture as is into commercially available feed for breeding sows.

It is also possible to mix it in advance when producing feed for breeding sows.

The Bacillus licheniformis bacteria contained in the feed for breeding sows of the present invention has 10 to 10 bacterial cells per kg of the feed.
It is preferable to add 111 pieces, preferably 10' to 10'2 pieces, and most preferably 105 to 1010 pieces.

The feed for breeding sows of the present invention exhibits excellent effects such as improving the survival rate of piglets from the first birth of breeding sows due to the action of Bacillus licheniformis bacteria contained therein.
Because it is extremely safe, it has high industrial value.

The present invention will be explained in more detail below with reference to Bacillus licheniformis preparation production examples and examples.

Production example [Preparation of Bacillus licheniformis live bacteria preparation] 100 parts by weight of tap water, 2 parts by weight of molasses, 1 part by weight of ammonium sulfate, 0.1 part by weight of dibasic potassium phosphate, 0 parts by weight of yeast extract,
A culture solution 11V'r in which 05 parts by weight of gold was dissolved and the pH was adjusted to 8.0 with 0.1N hydrochloric acid and 0.1N caustic soda.
Place in jar fermenter and sterilize (121°C, 115°C)
minutes), then pre-cultured Bacillus licheniformis strain MN-001 (Feikoken Jokyo No. 266 KMN-
001) was inoculated and cultured with shaking for 24 hours. Next, the bacterial cells were separated from the culture solution by centrifugation, and starch I Kft was added to and mixed with the bacterial cells, followed by drying to prepare a Bacillus licheniformis MN~o o 1 strain preparation (hereinafter referred to as MN-001 preparation). ) was obtained. The number of viable bacteria in this preparation was 10a/g.

Example Pregnant female pigs AF0 weighing 170 to 180 kg were used, divided into two groups with each group having a long-lived pig, and fed to each group. 60 days before calving, a control group (area with addition of tengun) and a group with addition of MN-001 preparation (preparation containing 108 viable bacteria per 1 g obtained using starch in the manufacturing example) were established. A feeding test was conducted for more than 80 days, up to 21 days later. The results are shown in Table 2.

(7) Table 1: Ingredients of Highbreed B Crude protein 150 grams or more Crude fat: 2.0 inches or more Crude fiber 10. '0% or less Coarse ash content: 10.0 chi or less Calcium 0.6% or more Lin 0.45% or more Digestible crude protein 11.5 or higher Total amount of digestible nutrients: 72.0 chi or more (8)

Claims (2)

[Claims] (1) A feed for breeding sows that improves the survival rate of newborn piglets, which is characterized by containing Bacillus licheniformis bacteria. (2) Bacillus licheniformis bacterium
The feed for breeding sows according to claim 1, which is Bacillus licheniformis strain MN-001 deposited as MN-001 in No. 6.