JPS60119458A - Electrophoresis device - Google Patents

Abstract

PURPOSE:To detect the case in which an air layer such as foam is interposed by providing a pair of electrodes separate from electrodes for migration in a migration tube and detecting the voltage value or current value between said electrodes. CONSTITUTION:An electrolyte is filled in a migration tube 2 and thereafter a power source 30 for detection is turned on to impress a voltage between a pair of electrodes 24 and 26 for detecting foam to supply constant current to the inside of the tube 2. The voltage at both terminals of the power source 30 is detected and is compared with a reference value (the value obtd. by adding some voltage to the value in the state of contg. no foam in the tube 2). When the detected voltage exceeds the reference value, an alarm bell 24 is sounded to announce that the tube 2 contains the foam. The operator opens thereupon the valve 21 of a discharge line 16 for the migration tube to feed the electrolyte to the inside of respective electrode cells 3, 4 while discharging the electrolyte and to deliver out the foam in succession thereto. When the foam in the tube 2 is eliminated and the detected voltage attains the reference voltage, there is no more output from the circuit 22. The electrophoresis of the sample is started in this state.

Description

[Detailed Description of the Invention] Kui〉Industrial Application Field This invention relates to an electrophoresis device, and more specifically, the present invention relates to an electrophoresis device that is equipped with an alarm means that issues an alarm when bubbles are present in an electrophoresis tube filled with an electrolyte. This is related to electrophoresis device H.

(B) Prior art In general, in electrophoresis devices such as a III-tube isokinetic electrophoresis device and a cell electrophoresis device, when filling an electrolyte flow path, for example, an electrophoresis tube with an electrolyte, Bubbles adhering to the inner peripheral wall surface of the wave channel may enter the electrophoresis tube together with the electrolyte, and even if electrophoresis is performed in this state, it is unlikely that the electrophoresis will be hindered.

However, since there has been no suitable method for detecting air bubbles in the electrophoresis tube, measurements are often performed with the air bubbles still present, resulting in a decrease in measurement accuracy.

(c) Purpose This invention was made in view of the above circumstances, and one of the main purposes is to be able to detect whether or not there are bubbles in an electrophoresis tube filled with electrolyte using electrical means. The goal is to

(d) Structure The structure of the present invention includes a pair of electrode layers, a migration tube interposed between these two electrodes, and a migration power supply connected to both electrodes of the electrode tank, and further includes At least one pair of electrodes different from both electrodes of the electrode tank inserted near both ends or into the electrode tank, a detection means for detecting a voltage value or a current value between the pair of electrodes, and a detection means for detecting a voltage value or a current value between the pair of electrodes; An electrophoresis device comprising a calculation means that compares detected outputs with reference values and issues an output when these detection outputs exceed the reference value, and an alarm means that issues an alarm when the calculation means produces an output. It is.

(e) Examples The present invention will be described in detail below based on examples shown in the drawings. Note that this invention is not limited to this.

FIG. 1 is a diagram showing the overall configuration of a cell electrophoresis device (1).

(2) has an inner diameter of 0.7++1m and a wall thickness of 0.3mmPi! A 770mm electrophoresis tube (31 + 41 is connected to both ends of this electrophoresis tube through a diaphragm (51 (6)), and (7) is connected to the electrode f81 (9) inside these. The constant current electrophoresis power supply (nO) is an optical electrophoresis speed measuring section.The electrode tank (3) +4) and the electrophoresis tube (2)
have liquid feeding paths ~03) and drainage paths 04) to 06) for the electrolytic solution and sample solution, respectively, and a ladder for feeding the electrolytic solution and sample solution is installed in these liquid feeding paths. A pump [171-09] is provided. Note that (20) and (21+) are valves for opening and closing the sample liquid feeding path and the draining path.

C3) G1!111 is a pair of detection electrodes inserted at both ends (1) of the electrophoresis tube (2), and is constructed by inserting a platinum needle through the pore of the electrophoresis tube (2). These detection electrodes are connected to a detection power source C310, which is an electrophoresis power source.

The capacitor is connected to both ends of this detection power supply, detects the voltage across it, and compares it with a preset reference value (a value determined based on the value when no bubbles or air layers are present, details will be described later). This is a liquid check circuit that serves as a detection/calculation means that valves the output when the detected voltage value exceeds the reference value, and this circuit receives the output from this circuit ( The alarm bell (■) is electrically connected to a liquid feeding control circuit C4 which also receives the output and stops the liquid feeding operation of the straining pumps (17) and 08) for dissolving liquid on both sides.

Next, the operation of the cell electrophoresis apparatus (1) having the above configuration will be explained.

First, the straining pump 08) for feeding the electrolytic solution is operated to feed the electrolytic solution into each electrode tank [3) +4).

After filling the electrophoresis tube (2) with each electrolytic solution, the detection power source ca[O is turned on and the detection electrode 125) 1
A voltage is applied between M to supply a constant current into the migration tube (2).

Therefore, the liquid check circuit (e) detects the detection power supply (3).
[Detect the voltage across 0 and compare it with a reference value (a value obtained by adding a slight voltage to the normal voltage when there are no bubbles in the migration tube (2)). Therefore, if this detected voltage is below the reference voltage, the normal voltage signal (output) of the circuit (a)
) to the liquid feeding control circuit to stop the operation of the squeezing pump surface 08).

Then, the sample liquid (electrophoresis liquid) is fed into the electrophoresis tube (2), and the sample ions are electrophoresed using the electrophoresis power source (ONL).

On the other hand, if the detected voltage exceeds or exceeds the reference voltage, an abnormal voltage signal (output) in the liquid check circuit
The alarm bell (4) will sound to notify that there are air bubbles in the migration tube (2).Therefore, open the valve (21) of the migration tube drain path 06) and drain each electrolyte while draining each electrolyte. Continuing to feed the electrolyte into each electrode tank +31 (4) and replenishing the electrolyte into the migration tube (2) 1. Then, when there are no bubbles in the migration tube (2), the voltage at both ends is the reference voltage. When the value returns to that value, that is, when the liquid check circuit (a) stops outputting, migration of sample ions is started as described above.

By configuring the cell electrophoresis device (1) as described above, it is possible to reliably detect the presence or absence of air bubbles in the electrophoresis tube (including the electrode tank) without malfunction, and it is possible to perform electrophoresis measurement with a high degree of vi. You can also know whether the electrophoresis tube (including the electrode tank) is filled with electrolyte.

In addition, as shown in Fig. 3, n electrodes (31) (31)...
may be inserted, in which case these electrodes may be inserted, e.g.
1st and 2nd electrode, 1st and 3rd electrode, etc.
...By combining the 1st and nth electrodes, the voltage (average value) at each electrode insertion part in the migration tube is detected and compared with the reference value, which can improve the accuracy of measuring the presence of bubbles. can.

Furthermore, as for the detection electrode, a platinum ring can be used in addition to the platinum needle in the embodiment.

For example, use a platinum ring (the inner diameter of which matches the inner diameter of the electrophoresis tube).

(thickness: 0.02 mm) is inserted coaxially in the middle of the electrophoresis tube. By using adjacent synthetic resin thin tubes such as Teflon as the migration tube, sealing can be achieved only by axial compression. In the embodiment shown in FIG. 1, the detection power supply is provided separately from the electrophoresis power supply, but the detection power supply may also be used as the electrophoresis power supply without having to be specially provided.

This invention detects a voltage value or a current value with at least one pair of electrodes inserted into an electrophoresis tube or an electrode tank,
This system detects abnormalities in the detection output of the electrolyte and issues an alarm.Based on the knowledge that the voltage or current value changes when there is an electrical layer such as a bubble in the electrolyte, The bubbles contained in the electrophoresis tube filled with electrophoresis can be reliably detected without malfunction, thereby enabling highly accurate electrophoresis measurement.

[Brief explanation of the drawing]

FIG. 1 is a configuration explanatory diagram showing one embodiment of an electrophoresis apparatus according to the present invention, and FIG. 2 is an explanatory diagram of detection electrode insertion positions showing another embodiment. (1)...Cell electrophoresis device, (2)...
...Electrophoresis tube, (7)...Electrophoresis power supply, Goji...
...liquid check circuit, prisoner...alarm bell. □
□□(c)...Detection electrode, 弼...Detection power supply.

Claims (1)

[Claims] 1. A migration tube interposed between a pair of electrodes, a migration tube interposed between these two electrodes, and a migration power supply connected to both electrodes of the electrode tank, and further equipped with a migration tube interposed between a pair of electrodes, and a migration power supply connected to both electrodes of the electrode tank, and further near both ends in the migration tube or in the electrode tank. at least one pair of electrodes different from both electrodes of the electrode tank, a detection means for detecting a voltage value or a current value between the pair of electrodes, and a detection output of this detection means as a reference value. An electrophoresis device comprising a computation means that compares and outputs an output when these detected outputs exceed a reference value, and an alarm means that issues an alarm when the computation means outputs an output.