JPS60115854A - Liquid chromatograph for multi-term analysis - Google Patents

Abstract

PURPOSE:To enable automatic analysis of multiple terms of one sample with one device by controlling automatically change-over of plural liquid vessels for a moving phase and a means for adjusting the moving phase. CONSTITUTION:The moving phase of the sample from an injecting part 6 is introduced into a column 13 when a cock 5 is changed over. The moving phase is separated by the water from a vessel 1 and the saccharoid content thereof is measured by a device 18 for measuring UV absorbancy. The moving phase is then introduced into a column 14 when the cock 5 is changed over. The moving phase is adjusted by a gradient device 3 and alpha-amino acid is separated. Said acid is measured by the device 18. The cock 5 is further changed over by which the moving phase is introduced into a column 15 where the fatty acid in the sample is similarly separated and measured. The sequence of such operations is preliminarily programmed and is accomplished automatically by a control part 21.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to a liquid chromatograph that performs multiple analysis on one sample.

(B) Prior art Conventionally, when analyzing multiple items of one sample using a liquid chromatograph, for example, analyzing multiple items such as α-amino acids, sugars, and fatty acids in large serum or food, each There is a problem that analysis is performed using a separate liquid chromatograph for each item, which requires an analyzer for each analysis item and is time-consuming. (c) Purpose This invention aims to improve the above problems. The purpose of this system is to provide a liquid chromatograph that can automatically analyze multiple items of a single sample.

B) Structure This invention connects in this order a plurality of mobile phase liquid containers, a mobile phase preparation means, a mobile phase liquid feeding pump, a switching cock, parallel different types of separation columns a switched by the switching cock, and a detector. At the same time, the sample injection unit is connected to the inlet of each separation column via a sample supply pipe with a branched on-off valve, and the operation of each system is automated so that multiple analysis items can be automatically performed sequentially on one sample. The present invention provides a liquid chromatograph for multi-item analysis, which is equipped with an automatic control section.

The liquid chromatograph of the present invention is provided with different types of separation columns used for each analysis item in parallel, has a means for preparing a mobile phase used for each analysis item, and transfers a sample to the separation column for each analysis item. It is characterized by having a sample supply tube with a sample container and an automatic control unit for automatically analyzing each analysis item in sequence, so that multiple items of one sample can be analyzed in one It has the advantage of being automatically analyzed on the machine.

(e) Example FIG. 1 shows a system diagram of a liquid chromatograph for multi-item analysis for measuring sugars, α-amino acids and fatty acids in large serum as an example of the liquid chromatograph of the present invention.

(1) and (2) are containers for water and methanol as mobile phase liquids, (3) is a gradient device that mixes the mobile phase liquids at a predetermined ratio to prepare the mobile phase, and (4) is a mobile phase liquid. Phase liquid pump, (5) is the switching cock, (6) is the sample injection part, (7), (8), and (9) are the sample supply pipes, and θ4 is the glycolytic column Zorbax OD S column (DuPont). ), θ is the length of the α-amino acid separation column.
5C-07/S 1504 (manufactured by Takasu Seisakusho), Qf9
is fatty acid separation column MBC-ODS (manufactured by Takasu Seisakusho)
, θQ is a column constant temperature bath, αη is a resistance tube, (to) is an ultraviolet absorbance measuring device, (0) is an absorbance measurement data processor, (e)
(b) is a drainage pipe and (b) is an automatic control unit that automatically controls the operation of this liquid chromatograph. Next, the operation of this liquid chromatograph will be explained.

Close the on-off valve QIQ) (6) and insert the sample injection part (6).
). As a result, the sample is divided into eight equal parts and enters the sample supply tubes (7), (s), and (9). Assuming that sugars, α-amino acids, and fatty acids in a sample are to be analyzed in this order, first, the temperature of each separation column is maintained at 40°C using a column constant temperature bath θ, and the switching cock (5) is switched to transfer the mobile phase to the saccharide decomposition column. Make sure that it only goes towards 0. Next, water as a mobile phase for glycolysis is passed from the water container (1) by a pump (4) through a gradient device (3), a switching cock (5), a glycolysis column α frame, and an ultraviolet absorbance measuring device (to). let After the sugar analysis system reaches a steady state, open the on-off valve 00 and send the sample to the inlet of the saccharide decomposition column O1, then open the on-off valve 0.
Close 0. The sample is drawn through the sugar column θ with water as the mobile phase, separated, and measured using an ultraviolet absorbance meter (g). Next, switch the switching cock (5) so that the mobile phase is directed only to the α-amino acid separation column α.
Using water and ethanol as mobile phase liquids, prepare a mobile phase for σ-amino acid separation of water/methanol (15/85 volume ratio) using a gradient device (3), and pump the mobile phase using a pump (4). . After the σ-amino acid analysis system reaches a steady state, open the on-off valve (1) and send the sample to the inlet of the α-amino acid separation column 0Φ, then close the on-off valve θη.

The sample is sent through the a-amino acid separation column using the above-mentioned water/methanol mixture as a mobile phase, separated, and measured using an ultraviolet absorbance measuring device. Next, switch the switching cock (5) so that the mobile phase goes only to the fatty acid separation column θ, and use the water/methanol (1
A mobile phase for fatty acid separation with a ratio of 0/90) is prepared and pumped using a pump (4). After the fatty acid analysis system reaches a steady state, open the enclosure valve a and transfer the sample to the fatty acid separation column Oυ.
After sending the liquid to the inlet of the valve, the on-off valve (6) is closed. As a result, the fatty acids in 1rk are separated and measured in the same manner as above. These operating procedures are programmed in advance and automatically performed by the automatic control unit Qυ.

According to the liquid chromatograph described above, sugars, α-amino acids, and fatty acids in a sample can be automatically analyzed by simply injecting one sample. In addition, other analytical items can be analyzed by changing the mobile phase and separation column to appropriate ones.

(f) Effect: According to the device of this invention, multiple items of one sample can be automatically measured with one device.

[Brief explanation of the drawing]

FIG. 1 is a system diagram of a liquid chromatograph according to an embodiment of the present invention. (1)...Water container, (2)...Methanol container, (3)...Gradient device, (4)...Mobile phase pump, (5)...Switching cock , (6)・
... Sample injection part, (7) (8 bar 9) ... Sample supply tube,
(11 (Iη@...on/off valve, (to)...sugar separation column, 04)...α-amino acid separation column, 00...fatty acid separation column, αQ...column constant temperature bath, 0η・・・・
Resistance tube, (g)...ultraviolet absorbance measuring device, Q groan...
Measured value processor, (a)...Drainage pipe, and υ...Automatic control unit.

Claims (1)

[Claims] 1. Multiple mobile phase liquid containers and mobile phase if! A mobile phase pump with manufacturing means, a switching cock, parallel different types of separation columns and detectors switched by this switching cock are connected in this order, and a sample injection part is installed at the inlet of each separation column, with a branched on-off valve. A liquid chromatograph for multi-item analysis that is connected via a sample supply tube and equipped with an automatic control section that automatically controls the operation of each system so that multiple-item analyzes can be automatically performed sequentially on one sample.