JPS60115854A - Liquid chromatograph for multi-term analysis - Google Patents
Liquid chromatograph for multi-term analysisInfo
- Publication number
- JPS60115854A JPS60115854A JP22502683A JP22502683A JPS60115854A JP S60115854 A JPS60115854 A JP S60115854A JP 22502683 A JP22502683 A JP 22502683A JP 22502683 A JP22502683 A JP 22502683A JP S60115854 A JPS60115854 A JP S60115854A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- moving phase
- mobile phase
- column
- liquid chromatograph
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/466—Flow patterns using more than one column with separation columns in parallel
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【発明の詳細な説明】
(イ)産業上の利用分野
この発明はひとつの試料について多項目の分析を行いつ
る液体クロマトグラフに関する。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to a liquid chromatograph that performs multiple analysis on one sample.
(ロ)従来技術
従来、液体クロマトグラフによって、例えば大血清や食
品中のα−アミノ酸、糖、脂肪酸などの多項目を分析す
るような、ひとつの試料について多項目を分析する場合
には、各項目毎に別個の液体クロマトグラフを用いて分
析がなされ、分析項目毎に分析器を要しかつ手数がかか
るという問題点があSo
(ハ) 目 的
この発明は上記問題点を改善するためになされたもので
、ひとつの試料の多項目を1台で自動的に分析しうる液
体クロマトグラフを提供することを目的とするものであ
る。(B) Prior art Conventionally, when analyzing multiple items of one sample using a liquid chromatograph, for example, analyzing multiple items such as α-amino acids, sugars, and fatty acids in large serum or food, each There is a problem that analysis is performed using a separate liquid chromatograph for each item, which requires an analyzer for each analysis item and is time-consuming. (c) Purpose This invention aims to improve the above problems. The purpose of this system is to provide a liquid chromatograph that can automatically analyze multiple items of a single sample.
に)構成
この発明は、複数の移動相用液体の容器と移動相調製手
段と移動相送液ポンプと切換えコックとこの切換えコッ
クにより切換えられる並列の異種の分離カラムaよび検
出器をこの順に連結するとともに、各分離カラムの入口
に試料注入部を、分岐した開閉弁付き試料供給管を介し
て連結し、かつひとつの試料について多項目の分析を順
次自動的に行えるよう各系統の作動を自動制御する自動
制御部を設けてなる多項目分析用液体クロマトグラフを
提供するものである。B) Structure This invention connects in this order a plurality of mobile phase liquid containers, a mobile phase preparation means, a mobile phase liquid feeding pump, a switching cock, parallel different types of separation columns a switched by the switching cock, and a detector. At the same time, the sample injection unit is connected to the inlet of each separation column via a sample supply pipe with a branched on-off valve, and the operation of each system is automated so that multiple analysis items can be automatically performed sequentially on one sample. The present invention provides a liquid chromatograph for multi-item analysis, which is equipped with an automatic control section.
この発明の液体クロマトグラフは、各分析項目毎に用い
られる異種の分離カラムが並列に設けられ、各分析項目
毎に用いられる移動相を調製する手段を有し、試料を分
析項目別に分離カラムに供給する試料容器付きの試料供
給管を有し、さらに各分析項目を順次自動的に分析する
ための自動制御部を設えていることを特徴とするもので
あり、ひとつの試料の多項目を1台で自動的に分析しつ
るという利点を有する。The liquid chromatograph of the present invention is provided with different types of separation columns used for each analysis item in parallel, has a means for preparing a mobile phase used for each analysis item, and transfers a sample to the separation column for each analysis item. It is characterized by having a sample supply tube with a sample container and an automatic control unit for automatically analyzing each analysis item in sequence, so that multiple items of one sample can be analyzed in one It has the advantage of being automatically analyzed on the machine.
(ホ)実施例
第1図に、この発明の液体クロマトグラフの一実施例と
して大血清中の糖、α−アミノ酸及び脂肪酸を測定しつ
る多項目分析用液体クロマトグラフの系統図を示した。(e) Example FIG. 1 shows a system diagram of a liquid chromatograph for multi-item analysis for measuring sugars, α-amino acids and fatty acids in large serum as an example of the liquid chromatograph of the present invention.
(1)と(2)はそれぞれ移動相用液体の水とメタノー
ルの容器、(3)は移動相用液体を所定の比率に混合し
て移動相を調製するグラジェント装置、(4)は移動相
送液ポンプ、(5)は切換えコック、(6)は試料注入
部、(7)(8)(9)は試料供給管、θ4は糖分解カ
ラムのゾルパックス(Zorbax) OD Sカラム
(デュポン社製)、θ轡はα−アミノ酸分離カラムのl
5C−07/S 1504 (高滓製作所製)、Qf9
は脂肪酸分離カラムMBC−ODS (高滓製作所製)
、θQはカラム恒温槽、αηは抵抗管、(至)は紫外線
吸光度測定器、0旧よ吸光度測定データ処理器、(ホ)
は排液管路及び(ロ)はこの液体クロマトグラフの作動
を自動制御する自動制御部である。次にこの液体クロマ
トグラフの作動を説明する。(1) and (2) are containers for water and methanol as mobile phase liquids, (3) is a gradient device that mixes the mobile phase liquids at a predetermined ratio to prepare the mobile phase, and (4) is a mobile phase liquid. Phase liquid pump, (5) is the switching cock, (6) is the sample injection part, (7), (8), and (9) are the sample supply pipes, and θ4 is the glycolytic column Zorbax OD S column (DuPont). ), θ is the length of the α-amino acid separation column.
5C-07/S 1504 (manufactured by Takasu Seisakusho), Qf9
is fatty acid separation column MBC-ODS (manufactured by Takasu Seisakusho)
, θQ is a column constant temperature bath, αη is a resistance tube, (to) is an ultraviolet absorbance measuring device, (0) is an absorbance measurement data processor, (e)
(b) is a drainage pipe and (b) is an automatic control unit that automatically controls the operation of this liquid chromatograph. Next, the operation of this liquid chromatograph will be explained.
開閉弁QIQ])(6)を閉じておいて試料注入部(6
)に試料を注入する。その結果試料は8等分されて試料
供給管(7)(s)(9)に入る。試料の糖、α−アミ
ノ酸及び脂肪酸をこの順に分析するとして、まず各分離
カラムの温度をカラム恒温槽θ呻によって40°Cに保
持し、切換えコック(5)を切換えて移動相が糖分解カ
ラム0にのみ向かうようにしておく。次いで糖分解用移
動相としての水を水の容器(1)からポンプ(4)によ
ってグラジェント装置(3)、切換えコック(5)、糖
分解カラムα枠及び紫外線吸光度測定器(至)を通過さ
せる。糖分析系が定常状態に達してから開閉弁00を開
き試料を糖分解カラムO1の入口部に送った後開閉弁0
0を閉じる。試料は移動相の水で糖分部カラムθ引こ送
られ分離されて紫外線吸光度測定器(ト)で測定される
。次いで切換えコック(5)を切換えて移動相がα−ア
ミノ酸分離カラムα傭このみ向かうようにしておいて、
移動相液体の水とエタノールとを用いグラジェント装置
(3)によって水/メタノール(15/85容量比)の
σ−アミノ酸分離用移動相を調製しポンプ(4)によっ
て該移動相を送液する。σ−アミノ酸分析系が定常状態
になってから開閉弁(1])を開き試料をα−アミノ酸
分離カラム0Φの入口部に送った後開閉弁θηを閉じる
。Close the on-off valve QIQ) (6) and insert the sample injection part (6).
). As a result, the sample is divided into eight equal parts and enters the sample supply tubes (7), (s), and (9). Assuming that sugars, α-amino acids, and fatty acids in a sample are to be analyzed in this order, first, the temperature of each separation column is maintained at 40°C using a column constant temperature bath θ, and the switching cock (5) is switched to transfer the mobile phase to the saccharide decomposition column. Make sure that it only goes towards 0. Next, water as a mobile phase for glycolysis is passed from the water container (1) by a pump (4) through a gradient device (3), a switching cock (5), a glycolysis column α frame, and an ultraviolet absorbance measuring device (to). let After the sugar analysis system reaches a steady state, open the on-off valve 00 and send the sample to the inlet of the saccharide decomposition column O1, then open the on-off valve 0.
Close 0. The sample is drawn through the sugar column θ with water as the mobile phase, separated, and measured using an ultraviolet absorbance meter (g). Next, switch the switching cock (5) so that the mobile phase is directed only to the α-amino acid separation column α.
Using water and ethanol as mobile phase liquids, prepare a mobile phase for σ-amino acid separation of water/methanol (15/85 volume ratio) using a gradient device (3), and pump the mobile phase using a pump (4). . After the σ-amino acid analysis system reaches a steady state, open the on-off valve (1) and send the sample to the inlet of the α-amino acid separation column 0Φ, then close the on-off valve θη.
試料は移動相の上記水/メタノール混合液でa −アミ
ノ酸分離カラム0傭こ送られ分離されて紫外線吸光度測
定器(ト)で測定される。次に切換えコック(5)を切
換えて移動相が脂肪酸分離カラムθ叶にのみ向かうよう
にしておいて、上記と同様の操作で水/メタノール(1
0/90谷蔗比)の脂肪酸分離用移動相を調製しポンプ
(4)によって送液する。脂肪酸分析系が定常状態にな
ってから囲閉弁a埠を開き試料を脂肪酸分離カラムOυ
の入口部に送った後開閉弁(6)を閉じる。その結果前
記と同様1rk憂i中の脂肪酸が分離測定される。そし
てこれらの操作手順は予めプログラムされて自動制御部
Qυによって自動的に行われる。The sample is sent through the a-amino acid separation column using the above-mentioned water/methanol mixture as a mobile phase, separated, and measured using an ultraviolet absorbance measuring device. Next, switch the switching cock (5) so that the mobile phase goes only to the fatty acid separation column θ, and use the water/methanol (1
A mobile phase for fatty acid separation with a ratio of 0/90) is prepared and pumped using a pump (4). After the fatty acid analysis system reaches a steady state, open the enclosure valve a and transfer the sample to the fatty acid separation column Oυ.
After sending the liquid to the inlet of the valve, the on-off valve (6) is closed. As a result, the fatty acids in 1rk are separated and measured in the same manner as above. These operating procedures are programmed in advance and automatically performed by the automatic control unit Qυ.
上記の液体クロマトグラフによればひとつの試料を注入
するだけで自動的に、試料中の糖、α−アミノ酸および
脂肪酸を分析できる。また、移動相と分離カラムとを適
切なものに変えれば他の分析項目も分析できる。According to the liquid chromatograph described above, sugars, α-amino acids, and fatty acids in a sample can be automatically analyzed by simply injecting one sample. In addition, other analytical items can be analyzed by changing the mobile phase and separation column to appropriate ones.
(へ)効果
この発明の装置によればひとつの試料の多項目を1台で
自動的に測定することができる1゜(f) Effect: According to the device of this invention, multiple items of one sample can be automatically measured with one device.
第1図はこの発明の一実施例の液体クロマトグラフの系
統図である。
(1)・・・水の容器、 (2)・・・メタノールの容
器、(3)・・・グラジェント装置、(4)・・・移動
相送液ポンプ、(5)・・・切換えコック、 (6)・
・・試料注入部、(7)(8バ9)・・・試料供給管、
(11(Iη@・・・開閉弁、(至)・・・糖分離カラ
ム、
04)・・・α−アミノ酸分離カラム、00・・・脂肪
酸分離カラム、 αQ・・・カラム恒温槽、0η・・・
抵抗管、(ト)・・・紫外線吸光度測定器、Q呻・・・
測定値処理器、
(イ)・・・排液管路、及びなυ・・・自動制御部。FIG. 1 is a system diagram of a liquid chromatograph according to an embodiment of the present invention. (1)...Water container, (2)...Methanol container, (3)...Gradient device, (4)...Mobile phase pump, (5)...Switching cock , (6)・
... Sample injection part, (7) (8 bar 9) ... Sample supply tube,
(11 (Iη@...on/off valve, (to)...sugar separation column, 04)...α-amino acid separation column, 00...fatty acid separation column, αQ...column constant temperature bath, 0η・・・・
Resistance tube, (g)...ultraviolet absorbance measuring device, Q groan...
Measured value processor, (a)...Drainage pipe, and υ...Automatic control unit.
Claims (1)
き移動相送液ポンプと切換えコックとこの切換えコック
により切換えられる並列の異種の分離カラムおよび検出
器をこの順に連結するとともに、各分離カラムの入口に
試料注入部を、分岐した開閉弁付き試料供給管を介して
連結し、かつひとつの試料について多項目の分析を順次
自動的に行えるよう各系統の作動を自動制御する自動制
御部を設けてなる多項目分析用液体クロマトグラフ。1. Multiple mobile phase liquid containers and mobile phase if! A mobile phase pump with manufacturing means, a switching cock, parallel different types of separation columns and detectors switched by this switching cock are connected in this order, and a sample injection part is installed at the inlet of each separation column, with a branched on-off valve. A liquid chromatograph for multi-item analysis that is connected via a sample supply tube and equipped with an automatic control section that automatically controls the operation of each system so that multiple-item analyzes can be automatically performed sequentially on one sample.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22502683A JPS60115854A (en) | 1983-11-28 | 1983-11-28 | Liquid chromatograph for multi-term analysis |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22502683A JPS60115854A (en) | 1983-11-28 | 1983-11-28 | Liquid chromatograph for multi-term analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS60115854A true JPS60115854A (en) | 1985-06-22 |
Family
ID=16822898
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP22502683A Pending JPS60115854A (en) | 1983-11-28 | 1983-11-28 | Liquid chromatograph for multi-term analysis |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS60115854A (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1089074A1 (en) * | 1999-10-01 | 2001-04-04 | Symyx Technologies | Parallel high-performance liquid chromatography with serial injection |
EP1194200A1 (en) * | 1999-04-23 | 2002-04-10 | Advanced Bioanalytical Services Inc. | High-throughput parallel liquid chromatography system |
US7790026B2 (en) | 2000-12-28 | 2010-09-07 | Cohesive Technologies Inc. | Multi column chromatography system |
JP2013507614A (en) * | 2009-10-08 | 2013-03-04 | ジーイー・ヘルスケア・リミテッド | Multi-stream high performance liquid chromatography module |
JP2013524212A (en) * | 2010-03-31 | 2013-06-17 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | Parallel separation system |
CN106324165A (en) * | 2015-07-02 | 2017-01-11 | 中国科学院大连化学物理研究所 | Method for detecting free fatty acids in trace amount of cell culture fluid |
JP2018173302A (en) * | 2017-03-31 | 2018-11-08 | 株式会社日立ハイテクサイエンス | Liquid chromatographic apparatus |
-
1983
- 1983-11-28 JP JP22502683A patent/JPS60115854A/en active Pending
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6296771B1 (en) | 1999-04-02 | 2001-10-02 | Symyx Technologies, Inc. | Parallel high-performance liquid chromatography with serial injection |
US6491816B2 (en) | 1999-04-02 | 2002-12-10 | Symyx Technologies, Inc. | Apparatus for parallel high-performance liquid chromatography with serial injection |
EP1194200A1 (en) * | 1999-04-23 | 2002-04-10 | Advanced Bioanalytical Services Inc. | High-throughput parallel liquid chromatography system |
EP1194200A4 (en) * | 1999-04-23 | 2004-04-07 | Advanced Bioanalytical Service | High-throughput parallel liquid chromatography system |
EP1089074A1 (en) * | 1999-10-01 | 2001-04-04 | Symyx Technologies | Parallel high-performance liquid chromatography with serial injection |
US7790026B2 (en) | 2000-12-28 | 2010-09-07 | Cohesive Technologies Inc. | Multi column chromatography system |
JP2013507614A (en) * | 2009-10-08 | 2013-03-04 | ジーイー・ヘルスケア・リミテッド | Multi-stream high performance liquid chromatography module |
US9063158B2 (en) | 2009-10-08 | 2015-06-23 | Ge Healthcare Limited | Multi-stream high-pressure liquid chromatography module |
JP2013524212A (en) * | 2010-03-31 | 2013-06-17 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | Parallel separation system |
CN106324165A (en) * | 2015-07-02 | 2017-01-11 | 中国科学院大连化学物理研究所 | Method for detecting free fatty acids in trace amount of cell culture fluid |
JP2018173302A (en) * | 2017-03-31 | 2018-11-08 | 株式会社日立ハイテクサイエンス | Liquid chromatographic apparatus |
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