JP2001074721A - Measuring device with temperature adjustment mechanism - Google Patents
Measuring device with temperature adjustment mechanismInfo
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- JP2001074721A JP2001074721A JP29430299A JP29430299A JP2001074721A JP 2001074721 A JP2001074721 A JP 2001074721A JP 29430299 A JP29430299 A JP 29430299A JP 29430299 A JP29430299 A JP 29430299A JP 2001074721 A JP2001074721 A JP 2001074721A
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- Prior art keywords
- temperature
- temperature adjustment
- column
- eluent
- measuring device
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Abstract
Description
【０００１】[0001]
【発明が属する技術分野】本発明は、試料中に含まれる
特定成分を測定するために用いるクロマトグラフィに関
する。詳しくは、装置を構成する主要部分の温度調整を
行い、常に一定の分析能を保持させることによって、精
度の高い測定結果を得るための測定装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a chromatography used for measuring a specific component contained in a sample. More specifically, the present invention relates to a measuring apparatus for adjusting the temperature of a main part constituting the apparatus and maintaining a constant analytical ability to obtain a highly accurate measurement result.
【０００２】[0002]
【従来の技術】液体クロマトグラフィを用いて試料中に
含まれる特定成分を測定する手段は、測定方法の中でも
最も精度の高い結果が得られるとして定評が高い。日常
的に行われる特定成分の測定としては、例えばグリコヘ
モグロビン、グリコアルブミンが病院の検査施設或いは
検査センタで行われている。液体クロマトグラフィは、
分析対象となる特定成分の種類によって、分離を行うた
めに最適なカラムが選択される。例えば赤血球中に存在
するグリコヘモグロビンを分析するために最適なカラム
が選別され、血清又は血漿中に存在するグリコアルブミ
ンを分析するために、最適なカラムが選別されて測定装
置が構成される。カラムの選別以外に最良な分析結果を
得るための検討も行われている。例えば、溶離液の成分
となる溶質の種類、濃度、溶離液のｐＨの調整、溶離液
の流れる速度によっても得られる分析結果が異なるた
め、特定成分の分離に最適な条件の検討が必要となる。
この様な液体クロマトグラフィにおける特定成分の分離
条件については、使用する測定者が設定された分離条件
を守らなければならない。測定者が設定された条件を守
らなければ、測定結果に差異を生じて精度が悪くなって
しまう。多くの場合、守るべき測定条件は測定装置によ
って制御されることになる。よって測定者は、測定装置
を操作する上で必要な分離条件を確実に守ることによっ
て精度の高い安定した測定結果が得られるはずである。
しかし、液体クロマトグラフィによって得られる特定成
分の分析結果であるクロマトグラムは、上述した場合以
外にも種々の測定環境条件によって影響を受ける。よっ
て、測定のために用いられる液体クロマトグラフィは、
管理された環境下において測定されることが望ましい。
上述した通り、同一の測定条件でなければ、得られる測
定結果は不正確になってしまう。測定環境条件の中でも
特に温度による影響は、十分注意しなければならない。
例えば、特定成分を吸着又は分離するための溶離液の温
度、前記溶離液の温度によって影響を受ける溶離液の流
量、特定成分のインジェクション時の温度、特定成分の
分離又は吸着が行われるカラムにおける温度等が原因と
なる場合がある。2. Description of the Related Art Means for measuring a specific component contained in a sample by using liquid chromatography has been well-established for obtaining the most accurate result among measurement methods. As a routine measurement of a specific component, for example, glycated hemoglobin and glycoalbumin are measured at a laboratory or a test center in a hospital. Liquid chromatography is
The optimum column for performing the separation is selected depending on the type of the specific component to be analyzed. For example, an optimal column is selected for analyzing glycated hemoglobin present in red blood cells, and an optimal column is selected for analyzing glycoalbumin present in serum or plasma to constitute a measuring device. In addition to column selection, studies are also underway to obtain the best analytical results. For example, the analysis results obtained vary depending on the type and concentration of the solute as a component of the eluent, the pH of the eluent, and the speed at which the eluent flows, so it is necessary to study the optimal conditions for separating specific components. .
As for the separation conditions of a specific component in such liquid chromatography, the measuring person to be used must adhere to the set separation conditions. If the measurer does not observe the set conditions, the measurement results will be different and accuracy will be degraded. In many cases, the measurement conditions to be observed will be controlled by the measuring device. Therefore, the measurer should be able to obtain a highly accurate and stable measurement result by surely keeping the separation conditions necessary for operating the measuring device.
However, a chromatogram which is an analysis result of a specific component obtained by liquid chromatography is affected by various measurement environmental conditions other than the case described above. Therefore, the liquid chromatography used for the measurement is
It is desirable to be measured in a controlled environment.
As described above, unless the measurement conditions are the same, the obtained measurement result will be inaccurate. Careful attention must be paid to the effects of temperature, especially among the measurement environment conditions.
For example, the temperature of an eluent for adsorbing or separating a specific component, the flow rate of an eluent affected by the temperature of the eluent, the temperature at the time of injection of a specific component, the temperature in a column where separation or adsorption of a specific component is performed Etc. may be the cause.
【０００３】液体クロマトグラフィによる測定は、試料
中に存在する特定成分の測定が、カラムによって分離さ
れることにより行われている。前記カラムによる分離
は、分離する試料そのままでは特定成分濃度が非常に高
く、分離が完全に行えなくなるため、前もって試料を希
釈液によって希釈をする方法が一般的である。前記希釈
の後、インジェクションを行うまでに希釈試料溶液の温
度を一定にするためのヒータが必要であり、それ以外に
もカラムが保持される部分に、カラム恒温槽を設けて、
前記恒温槽をヒータによって温度調整する必要があっ
た。また、検出器も環境温度の影響を受けるので、同一
温度で測定する方が望ましいため、検出器も温度調整し
なければならない。従来の測定装置の場合、これら３つ
のヒータは別個独立しており、別々に温度調整すること
で分離条件を一定に保つ役割を果たしていた。[0003] The measurement by liquid chromatography is performed by measuring a specific component present in a sample by separating it with a column. In the separation using the column, since the concentration of a specific component is extremely high if the sample to be separated as it is, separation cannot be performed completely. Therefore, a method of diluting the sample with a diluent in advance is generally used. After the dilution, a heater is required to keep the temperature of the diluted sample solution constant until injection is performed, and a column in which a column is held other than that is provided with a column thermostat,
It was necessary to adjust the temperature of the constant temperature bath with a heater. In addition, since the detector is also affected by the environmental temperature, it is desirable to perform measurement at the same temperature. Therefore, the temperature of the detector must be adjusted. In the case of the conventional measuring device, these three heaters are independent and independent, and play a role of keeping the separation conditions constant by adjusting the temperature separately.
【０００４】これら別個独立された場所で温度調整され
ている従来法に対して、特開平９−２６４８９０号で
は、試料溶液を温度調整するためのヒータと、特定成分
を分離するためのカラムと、分離された特定成分を検出
する検出器を単一の温度調整用オーブン中に設置させて
温度調整を行う発明が記載されている。具体的な温度調
整用のオーブンの記載として底板に発熱体を用いた箱状
のものが紹介されている。温度調整は、温度調整を行う
範囲が同一の温度で調整される必要がある。[0004] In contrast to the conventional method in which the temperature is adjusted in these separate and independent places, Japanese Patent Application Laid-Open No. 9-264890 discloses a heater for adjusting the temperature of a sample solution, a column for separating specific components, and There is described an invention in which a detector for detecting a separated specific component is installed in a single temperature adjusting oven to adjust the temperature. As a specific description of an oven for temperature adjustment, a box-shaped one using a heating element for a bottom plate is introduced. In the temperature adjustment, it is necessary that the temperature adjustment range is adjusted at the same temperature.
【０００５】[0005]
【発明が解決しようとする課題】従来の液体クロマトグ
ラフィによる測定装置には、温度調整を行っている場所
が、上述した３カ所しか存在していなかった。希釈液に
よって希釈された試料がカラムに到達されるまでの間に
は、溶離液供給ユニット、送液ポンプ、インジェクショ
ンバルブ、プレフィルタが存在する。前記溶離液供給ユ
ニット、送液ポンプ、インジェクションバルブも、測定
環境温度による影響を大きく受けており、溶離液の流量
を不安定にさせて、測定結果に影響を及ぼしていた。更
に、前記特開平９−２６４８９０号に記載されているオ
ーブンを用いる方法では、温度調整を行う範囲が広範囲
に及ぶため、発熱体を内存する大きな底板が必要になる
ので、一定温度を保つために必要なエネルギーが大きく
なり、コストの負担が大きくなってしまう。また、大き
な底板の使用は、測定装置の重量を増すことにもなる。
前記溶離液供給ユニット、送液ポンプ、インジェクショ
ンバルブ、プレフィルタ、カラム保持部をも含めた広範
囲の温度調整を行う事を目的として、常時安定したクロ
マトグラムを得ることができるためものであって、一定
温度を維持するために必要なコストを押さえ、しかも測
定装置の重量が増加しない機構を備えた測定装置が望ま
れていた。In a conventional measuring apparatus using liquid chromatography, there are only three places for temperature adjustment as described above. Before the sample diluted by the diluent reaches the column, an eluent supply unit, a liquid feed pump, an injection valve, and a prefilter are present. The eluent supply unit, the liquid feed pump, and the injection valve are also greatly affected by the temperature of the measurement environment, and the flow rate of the eluent is made unstable to affect the measurement result. Further, in the method using an oven described in Japanese Patent Application Laid-Open No. 9-264890, the temperature adjustment range is wide, so a large bottom plate including a heating element is required. The required energy increases and the cost burden increases. The use of a large bottom plate also increases the weight of the measuring device.
The eluent supply unit, liquid sending pump, injection valve, pre-filter, for the purpose of performing a wide range of temperature adjustment including the column holding unit, because it is possible to always obtain a stable chromatogram, There has been a demand for a measuring device provided with a mechanism that reduces the cost required for maintaining a constant temperature and does not increase the weight of the measuring device.
【０００６】[0006]
【課題を解決するための手段】上述した課題を解決する
ために、液体クロマトグラフィの装置を構成している溶
離液供給ユニット、送液ポンプ、インジェクションバル
ブ、プレフィルタ、カラム保持部を温度調整ボックスに
格納し、前記温度調整ボックス内に温風を送風すること
によって安定したクロマトグラムを得るための温度調整
を行っている。この様に温風によって前記各部を温度調
整する方法が、非常に効果的であることが、検討の結果
判明した。前記カラム保持部は、温風によって管理され
る事が望ましいが、更に前記カラム保持部が独自の温度
調整機構を設けて、温度調整ができる場合であっても良
い。検出器も、温風の温度と、特定成分を検出するため
の検出器における温度が異なる場合は、それぞれ別の温
度調整機構を設けて温度調整してもかまわない。また、
脱気装置も一緒に温風によって温度調整しても良い。In order to solve the above-mentioned problems, an eluent supply unit, a liquid feed pump, an injection valve, a pre-filter, and a column holding unit constituting a liquid chromatography apparatus are provided in a temperature control box. The temperature is adjusted for storing a stable chromatogram by storing and storing warm air in the temperature adjustment box. As a result of investigation, it has been found that the method of adjusting the temperature of each section with warm air is very effective. The column holding section is desirably controlled by hot air, but the column holding section may be provided with its own temperature adjusting mechanism to adjust the temperature. When the temperature of the hot air is different from the temperature of the detector for detecting the specific component, the temperature of the detector may be adjusted by providing separate temperature adjusting mechanisms. Also,
The temperature of the deaerator may be adjusted together with warm air.
【０００７】本発明で言う温度調整ボックスとは、金属
又は合成樹脂のパネルを組み合わせた箱状の隔離された
ものを示し、この隔離された環境中に溶離液供給ユニッ
ト、送液ポンプ、インジェクションバルブ、プレフィル
タ、カラム保持部を内蔵させるためのボックスを言う。
前記温度調整ボックスに、更に脱気装置及び検出器を内
蔵させても良い。温度の管理方法は、温度調整ボックス
に、一定温度に調整された温風をあてることによって、
温度調整ボックス内の温度調整をおこなう。カラム保持
部、検出器が独自の温度調整機構を持ち、更に温風によ
って温度調整を行っても良い。この時、カラム保持部の
温度又は検出器の温度と、温風が別々の温度で管理され
てもかまわない。The term "temperature control box" as used in the present invention refers to a box-shaped isolated unit formed by combining metal or synthetic resin panels. In this isolated environment, an eluent supply unit, a liquid feed pump, and an injection valve are provided. , A pre-filter, and a box for incorporating a column holding unit.
The temperature adjustment box may further include a degassing device and a detector. The temperature management method is to apply hot air adjusted to a certain temperature to the temperature adjustment box,
Adjust the temperature in the temperature adjustment box. The column holding section and the detector may have their own temperature adjustment mechanisms, and may further adjust the temperature with hot air. At this time, the temperature of the column holding unit or the temperature of the detector and the hot air may be managed at different temperatures.
【０００８】[0008]
【発明の実施の形態】（実施形態１）本発明の液体クロ
マトグラフィによる測定装置の例を以下に示す。血液試
料中に含まれるヘモグロビンＡ１ｃを、高速液体クロマ
トグラフィによる測定装置で測定を行った。測定装置
は、溶離液ボトルＡ、溶離液ボトルＢ内にある溶離液
Ａ、溶離液Ｂの各溶離液が、脱気装置２、溶離液切換え
バルブ３ａ，３ｂ及びマニホールド４からなる溶離液供
給ユニットを介して送液ポンプ５によって吸引される。
マニホールド４では溶離液Ａ、Ｂが混合される。送液ポ
ンプを通過した溶離液Ａ及びＢは、試料導入部７から送
られてくる血液試料と共にインジェクションバルブに入
り、血液試料のインジェクションに続いて溶離液Ａ及び
Ｂが送液される。続いてカラム恒温槽９内にあるプレフ
ィルタ６及びカラム７を通り、カラム７の下流側に配置
された分光光度計１０と、この装置を制御する制御部１
１とから構成されている。(Embodiment 1) An example of a measuring apparatus using liquid chromatography of the present invention is shown below. Hemoglobin A1c contained in the blood sample was measured by a measuring device using high performance liquid chromatography. The measuring device is an eluent supply unit including an eluent A, an eluent switching valve 3a, 3b, and a manifold 4 in which each of the eluents A and B in the eluent bottle A and eluent bottle B is used. Is sucked by the liquid feed pump 5 through
In the manifold 4, eluents A and B are mixed. The eluents A and B that have passed through the liquid sending pump enter the injection valve together with the blood sample sent from the sample introduction unit 7, and the eluents A and B are sent following the injection of the blood sample. Subsequently, the light passes through the pre-filter 6 and the column 7 in the column thermostat 9, and is disposed on the downstream side of the column 7 with the spectrophotometer 10 and the control unit 1 for controlling the apparatus.
And 1.
【０００９】溶離液ボトルＡ及び溶離液ボトルＢには、
それぞれ溶離液Ａ及び溶離液Ｂが収容されており、溶離
液Ａ及び溶離液Ｂは濃度の異なるリン酸緩衝液である。
これらの溶離液Ａ、溶離液ＢのヘモグロビンＡ１ｃに対
する溶出力は、溶離液Ａ、溶離液Ｂの順で大きくなって
いる。溶離液切換えバルブ５ａ，５ｂは、制御部１１に
よって開閉制御され、常にどちらか一つが開き、溶離液
ＡとＢの割合を変えてカラム７へ供給することによって
ヘモグロビンＡ１ｃの分離を行っている。The eluent bottle A and the eluent bottle B include:
An eluent A and an eluent B are accommodated, respectively, and the eluents A and B are phosphate buffer solutions having different concentrations.
The dissolution power of these eluents A and B in hemoglobin A1c increases in the order of eluent A and eluent B. The eluent switching valves 5a and 5b are controlled to be opened and closed by the control unit 11, and one of them is always open, and the hemoglobin A1c is separated by supplying the column 7 with the ratio of the eluents A and B changed.
【００１０】送液ポンプ５は、プランジャ型ポンプであ
り、溶離液を３０〜２００ｋｇ／ｃｍ３の圧力範囲内で
カラム７へ送液している。インジェクションバルブ８
は、六方バルブで、試料導入部７より導入される血液試
料を、カラムに続く送液管内に注入される。カラム６
は、イオン交換カラム又は逆相分配カラムが用いられ
る。カラム恒温槽９は、カラム６の温度を一定に調整し
ている。分光光度計１０は、カラム６から分離されて溶
出される血液試料の各成分の吸光度を測定するもので、
測定波長は４１５ｎｍである。The liquid sending pump 5 is a plunger type pump, and sends the eluent to the column 7 within a pressure range of 30 to 200 kg / cm 3 . Injection valve 8
Is a six-way valve for injecting the blood sample introduced from the sample introduction part 7 into the liquid feed pipe following the column. Column 6
For the method, an ion exchange column or a reversed phase distribution column is used. The column thermostat 9 regulates the temperature of the column 6 to be constant. The spectrophotometer 10 measures the absorbance of each component of the blood sample separated and eluted from the column 6,
The measurement wavelength is 415 nm.
【００１１】制御部１１は、内蔵するマイクロコンピュ
ータによって本装置の各部分を制御して作動させるとと
もに、分析条件を最適化させるためのものである。制御
部の読み出し専用メモリには、予め、分析条件の変化に
対するＨｂＡ１ｃの溶出時間の変化量の関係を示すチャ
ートが格納されている。分析条件は溶離液切り換えバル
ブ３ｂから３ａへの切り換え時間によって制御されてい
る。The control unit 11 controls and operates each part of the apparatus by a built-in microcomputer and optimizes analysis conditions. The read-only memory of the control unit previously stores a chart showing the relationship between the change in the elution time of HbA1c and the change in the analysis conditions. The analysis conditions are controlled by the switching time of the eluent switching valve 3b to 3a.
【００１２】本発明は、上述した溶離液供給ユニット、
送液ポンプ、インジェクションバルブ、プレフルタ、カ
ラム保持部を、温度調整ボックスに内蔵している。温度
調整ボックスは合成樹脂によって作製し、温度調整方法
は、４０℃に加熱された空気をファンモータによって温
度調整ボックス内に送風する。この際温度調整ボックス
には、送風入口と出口によって温風を循環させている。The present invention provides an eluent supply unit as described above,
A liquid feed pump, an injection valve, a pre-filter, and a column holding unit are built in the temperature adjustment box. The temperature adjustment box is made of a synthetic resin, and the temperature adjustment method is to blow air heated to 40 ° C. into the temperature adjustment box by a fan motor. At this time, warm air is circulated through the temperature adjustment box through the air inlet and outlet.
【００１３】[0013]
【発明の効果】液体クロマトグラフィの装置を構成して
いる溶離液離供給ユニット、送液ポンプ、インジェクシ
ョンバルブ、プレフィルタ、カラム保持部を温度調整ボ
ックスに格納し、前記温度調整ボックス内に温風を送風
することによって、安定したクロマトグラムを得ること
ができた。According to the present invention, the eluent separating / supplying unit, the liquid sending pump, the injection valve, the pre-filter, and the column holding part constituting the liquid chromatography apparatus are stored in a temperature control box, and hot air is blown into the temperature control box. By blowing air, a stable chromatogram could be obtained.
【図１】 溶離液供給ユニット、送液ポンプ、インジェ
クションバルブ、プレフィルタ、カラム保持部を温度調
整ボックスに格納した液体クロマトグラフィの概略図で
ある。FIG. 1 is a schematic diagram of liquid chromatography in which an eluent supply unit, a liquid feed pump, an injection valve, a prefilter, and a column holding unit are stored in a temperature adjustment box.
Claims (4)
マトグラフィによって測定する装置であって、溶離液供
給ユニット、送液ポンプ、インジェクションバルブ、プ
レフィルタ、カラム保持部が、温風によって温度調整さ
れることを特徴とする測定装置。An apparatus for measuring a specific component contained in a sample by liquid chromatography, wherein an eluent supply unit, a liquid sending pump, an injection valve, a prefilter, and a column holding unit are temperature-controlled by hot air. A measuring device, characterized in that:
マトグラフィによって測定する装置であって、脱気装
置、溶離液供給ユニット、送液ポンプ、インジェクショ
ンバルブ、プレフィルタ、カラム保持部が、温風によっ
て温度調整されることを特徴とする測定装置。2. An apparatus for measuring a specific component contained in a sample by liquid chromatography, wherein a degassing device, an eluent supply unit, a liquid sending pump, an injection valve, a prefilter, and a column holding section are heated with hot air. A temperature measuring device characterized in that the temperature is adjusted by:
マトグラフィによって測定する装置であって、溶離液供
給ユニット、送液ポンプ、インジェクションバルブ、プ
レフィルタ、カラム保持部、検出器が、温風によって温
度調整されることを特徴とする測定装置。3. An apparatus for measuring a specific component contained in a sample by liquid chromatography, wherein an eluent supply unit, a liquid sending pump, an injection valve, a prefilter, a column holding unit, and a detector are heated by hot air. A measuring device characterized in that the temperature is adjusted.
マトグラフィによって測定する装置であって、脱気装
置、溶離液供給ユニット、送液ポンプ、インジェクショ
ンバルブ、プレフィルタ、カラム保持部、検出器が、温
風によって温度調整されることを特徴とする測定装置。4. An apparatus for measuring a specific component contained in a sample by liquid chromatography, comprising: a deaerator, an eluent supply unit, a liquid pump, an injection valve, a prefilter, a column holder, and a detector. A measuring device characterized in that the temperature is adjusted by hot air.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29430299A JP4318127B2 (en) | 1999-09-07 | 1999-09-07 | Measuring device with temperature adjustment mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29430299A JP4318127B2 (en) | 1999-09-07 | 1999-09-07 | Measuring device with temperature adjustment mechanism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001074721A true JP2001074721A (en) | 2001-03-23 |
| JP4318127B2 JP4318127B2 (en) | 2009-08-19 |
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ID=17805947
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29430299A Expired - Lifetime JP4318127B2 (en) | 1999-09-07 | 1999-09-07 | Measuring device with temperature adjustment mechanism |
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| Country | Link |
|---|---|
| JP (1) | JP4318127B2 (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004083847A1 (en) * | 2003-03-17 | 2004-09-30 | Hitachi High-Technologies Corporation | Liquid chromatograph and pre-heat condition setting method |
| JP2005106813A (en) * | 2003-09-09 | 2005-04-21 | Tosoh Corp | Liquid chromatographic apparatus |
| JP2005300189A (en) * | 2004-04-07 | 2005-10-27 | Japan Organo Co Ltd | Liquid chromatograph |
| US7374674B2 (en) | 2003-09-09 | 2008-05-20 | Tosoh Corporation | Liquid chromatography apparatus |
| WO2010026742A1 (en) | 2008-09-02 | 2010-03-11 | ジーエルサイエンス株式会社 | Liquid chromatograph |
| JP2017116350A (en) * | 2015-12-22 | 2017-06-29 | 株式会社島津製作所 | Passage mechanism and liquid chromatograph with the same |
| JP2017161295A (en) * | 2016-03-08 | 2017-09-14 | 東ソー株式会社 | Liquid sending device for liquid chromatograph |
| CN110075568A (en) * | 2012-08-24 | 2019-08-02 | 通用电气健康护理生物科学股份公司 | The system and method for controlling liquid chromatographic system |
| US11573212B2 (en) | 2018-06-18 | 2023-02-07 | Shimadzu Corporation | Flow channel mechanism and liquid chromatograph including the same |
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1999
- 1999-09-07 JP JP29430299A patent/JP4318127B2/en not_active Expired - Lifetime
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004083847A1 (en) * | 2003-03-17 | 2004-09-30 | Hitachi High-Technologies Corporation | Liquid chromatograph and pre-heat condition setting method |
| JP2005106813A (en) * | 2003-09-09 | 2005-04-21 | Tosoh Corp | Liquid chromatographic apparatus |
| US7374674B2 (en) | 2003-09-09 | 2008-05-20 | Tosoh Corporation | Liquid chromatography apparatus |
| JP4561249B2 (en) * | 2003-09-09 | 2010-10-13 | 東ソー株式会社 | Liquid chromatograph |
| JP2005300189A (en) * | 2004-04-07 | 2005-10-27 | Japan Organo Co Ltd | Liquid chromatograph |
| JP4504721B2 (en) * | 2004-04-07 | 2010-07-14 | オルガノ株式会社 | Liquid chromatography equipment |
| WO2010026742A1 (en) | 2008-09-02 | 2010-03-11 | ジーエルサイエンス株式会社 | Liquid chromatograph |
| JPWO2010026742A1 (en) * | 2008-09-02 | 2012-06-14 | ジーエルサイエンス株式会社 | Liquid chromatograph |
| CN110075568A (en) * | 2012-08-24 | 2019-08-02 | 通用电气健康护理生物科学股份公司 | The system and method for controlling liquid chromatographic system |
| JP2017116350A (en) * | 2015-12-22 | 2017-06-29 | 株式会社島津製作所 | Passage mechanism and liquid chromatograph with the same |
| JP2017161295A (en) * | 2016-03-08 | 2017-09-14 | 東ソー株式会社 | Liquid sending device for liquid chromatograph |
| US11573212B2 (en) | 2018-06-18 | 2023-02-07 | Shimadzu Corporation | Flow channel mechanism and liquid chromatograph including the same |
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|---|---|
| JP4318127B2 (en) | 2009-08-19 |
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