JPS5985225A - Culture medium for culturing mushroom - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The invention relates to a culture medium for use in the artificial cultivation of mushroom kernels. More specifically, the present invention relates to a culture medium for cultivating mushrooms, characterized in that a hydrogel made of a water-absorbing non-electrolyte polymer is mixed into the culture substrate.

Traditionally, most edible mushrooms were grown using single-pot hydroponics using logs, and the harvest was often influenced by climate conditions.

In recent years, an artificial bed cultivation method has been established for cultivating enokida mushrooms, oyster mushrooms, nameko mushrooms, pine mushrooms, wood shimeji mushrooms, etc. in boxes or bottles using a culture medium mixed with sawdust and rice bran. It is now possible to stably harvest edible mushrooms without being affected by the seasons. Artificial cultivation of mushrooms, which used to be a domestic labor for farmers, is now transitioning to an industrial production format that involves continuous mass cultivation. For example, sawdust, rice husk, rice bran, etc. are used as the mixed material for this artificial cultivation.

In the cultivation of mushrooms, sawdust and rice husk are used as nutrients [7
Although it is rarely used, it creates a gap in the cultivation bottle, retains air, and absorbs the acid necessary for the growth of mycelia, as well as adsorbs moisture and prevents the surface of the fungus bed from drying out. However, Gakuzu is a natural product, and its water retention capacity varies depending on the tree species and freshness. Sawdust with low sexiness or dry sawdust not only causes poor growth of mycelium and fruiting bodies, but also
Sometimes it can cause the development of harmful bacteria. As described above, it is necessary to use sawdust with excellent water retention properties, but it is becoming difficult to do so manually. In addition, sawdust is similar to rice bran, and the fact that fresh sawdust must be used and removed is an important point in the method of artificially cultivating mushrooms using sawdust. As a result of various studies to solve the drawbacks of this method, we found that a highly water-absorbent hydrogel is extremely effective as a mixed material for the culture medium in the conventional method (Special 6 Do(1□+11?) 57-96707).

Further investigation into this technology revealed that polyelectrolyte-based hydrogels have a high water absorption rate and are excellent materials for use as hydrogels added to the mushroom culture medium phase. We found that it is susceptible to changes depending on the situation and is difficult to use in some cases.

The present inventors studied culture substrates that improved this point and arrived at the present invention.

That is, the present invention is a culture medium for cultivating mushrooms, characterized in that the culture medium is mixed with a hydrogel consisting of a water-absorbing, non-1η dissolving polymer.

Hydrogels made of water-absorbing, non-ttt-degrading polymers generally have low water absorption rates, but are less likely to be affected by other formulation components.

By using the Baitoshi base material of the present invention, enoki mushroom,
Oyster mushrooms, nameko mushrooms, pine mushrooms, shimeji mushrooms, and the like can be easily and stably produced by artificial cultivation in nurseries, and it is possible to increase the yield of oyster mushrooms, shorten the cultivation period, and improve quality.

The culture substrate used in the present invention consists of a material that provides water retention and air permeability, such as sawdust, rice hulls, and straw, and a monthly feed that serves as a nutrient source, such as rice bran.

The water-absorbing substance used in the present invention absorbs water and swells, but does not dissolve in water, and
There is no particular restriction as long as it is a physically or chemically crosslinked non-ntmw polymer, and it may be a natural product or a synthetic product.

Examples include crosslinked polyethylene oxide, crosslinked polyvinyl alcohol, crosslinked polyamide, crosslinked dimple, carrageenan, and the like.

In particular, cross-linked polyethylene 1 oxide has a large swelling capacity with water and is easily available, making it particularly suitable for use in the present invention. In addition, it is preferable that the hydrogel made of the water-absorbing material (1) does not exhibit a pasty appearance (stickiness), and the shape is suitably granular or flaky.The reason for this is not fully clear, but the gaps in the culture medium It is presumed that this is because water retention and oxygen supply suitable for mushroom cultivation are facilitated without decreasing the amount of water.

A water-absorbing hydrogel that does not have a pasty appearance is suitable; on the other hand, if it becomes pasty, the hydrogel will not be able to receive enough oxygen, causing overhumidity, making it unsuitable for this cultivation. . Furthermore, whether or not a hydrogel made of an aqueous formula exhibits a paste-like appearance is controlled by the size of the hydrogel and its strength. If the hydrogel has fine particles, it will have a paste-like appearance. On the other hand, if the hydrogel particles are sufficiently large, they are less likely to become pasty.

The presence or absence of glue-like appearance was investigated depending on the particle size and strength of the hydrogel.

If the particle size of the hydrogel is 100 μm or more, it will not exhibit a pasty appearance. Also, if the strength of human locale is compressive strength,
It was found that if it was 1.00 f/cl1 or higher, it would not exhibit a pasty appearance.

Furthermore, the use of the water-absorbent hydrogel used in the present invention varies depending on the type of mushroom to be cultivated. In the case of enoki mushrooms, the water content of the culture medium is preferably adjusted to 60-7096. If the number of users of the hydrogel is too small, it will be less effective. On the other hand, if there is too much, the culture medium becomes overhumidified, which is explained in terms of fungi.

Put the water-absorbing material ``l'' into water, and add the hydrogel to the water.
'Jru. Next, it is preferable to use this hydrogel by thoroughly mixing it into the commonly used culture co-phase (7).
There is no particular restriction, and water may be added after uniformly mixing the culture substrate and the water-absorbing substance.

In addition, other advantages of the tree invention are (1) the optimal water content range of the culture medium for mushroom cultivation is widened, and v4Pji of moisture is facilitated, (2) a stable culture medium for mushroom cultivation can be obtained. (8) The growth of hyphae is stable, and (4) microscopic fertilizers, hormones, etc. can be added.

EXAMPLES Hereinafter, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited thereto.

Example 1 (Culture of enokitake mushrooms) 400 g of junk, 100 fl of rice bran, 80 g of water, two water-absorbent substances 'R (cross-linked polyethylene) oxide, water absorption capacity 4
09/I) Add and mix 50fl culture medium to Yongfei 5oo
- after filling into plastic bottles and sealing them 1
High-pressure sterilization is performed at 20°C for 80 minutes. Thereafter, the enokitake fungus is cultured in a culture room at a temperature of 7.17 to 18°C and a humidity of 60 to 6,596°C. Once the mycelium grows throughout the bottle, scrape the fungus. Thereafter, the mushrooms are allowed to sprout at 12-13° C. and a humidity of 80-8596°C, and finally grown in a growth room.

The room temperature in the growth room is 5-7℃, and the humidity is 80-8596.
Make it.

As a control example, a culture medium prepared by mixing 400 g of sawdust, 100 g of rice bran, and 80 g of water was used, and Table 1 shows the results of determining the yield, growth period, and quality of enokitake fruiting bodies in the above examples and control examples.

\, - \-\ ~゛-・4 \-\ ~\\\ \ to 2, \\ Hehe ~ p (St. It can be seen that J1 has been revised compared to the conventional method.

Claims (1)

[Scope of Claims] (1) Culture medium]) is mixed with a gel made of a water-absorbing non-electrolytic PJ￥ff polymer, and t is a 4″ mark. Base. □
(2) Claims rrx in which the particle size of the hydrogel made of a water-absorbing non-electrolyte polymer is 1 (10μ or more - J)
1 x medium for cultivating mushrooms according to item 1. (3) YJF in which the water-absorbing substance is a natural product or a synthetic product.
ii'l'nl'I A culture medium for cultivating mushrooms according to the requirements of the requirements.