JPS5970963A - 狭径微粒子カラムの充填方法及びカラム - Google Patents
狭径微粒子カラムの充填方法及びカラムInfo
- Publication number
- JPS5970963A JPS5970963A JP58154255A JP15425583A JPS5970963A JP S5970963 A JPS5970963 A JP S5970963A JP 58154255 A JP58154255 A JP 58154255A JP 15425583 A JP15425583 A JP 15425583A JP S5970963 A JPS5970963 A JP S5970963A
- Authority
- JP
- Japan
- Prior art keywords
- column
- slurry
- diameter
- filling
- narrow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002245 particle Substances 0.000 title claims description 64
- 238000000034 method Methods 0.000 title claims description 59
- 239000002002 slurry Substances 0.000 claims description 55
- 238000012856 packing Methods 0.000 claims description 45
- 239000002904 solvent Substances 0.000 claims description 34
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 17
- 238000004811 liquid chromatography Methods 0.000 claims description 16
- 238000004817 gas chromatography Methods 0.000 claims description 14
- 239000005350 fused silica glass Substances 0.000 claims description 13
- 239000000945 filler Substances 0.000 claims description 10
- 239000010935 stainless steel Substances 0.000 claims description 9
- 229910001220 stainless steel Inorganic materials 0.000 claims description 9
- 229910000831 Steel Inorganic materials 0.000 claims description 4
- 239000010959 steel Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims 2
- 230000003750 conditioning effect Effects 0.000 claims 1
- 239000000470 constituent Substances 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 description 21
- 238000004587 chromatography analysis Methods 0.000 description 14
- 238000009826 distribution Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000005526 G1 to G0 transition Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000010419 fine particle Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- WDECIBYCCFPHNR-UHFFFAOYSA-N chrysene Chemical compound C1=CC=CC2=CC=C3C4=CC=CC=C4C=CC3=C21 WDECIBYCCFPHNR-UHFFFAOYSA-N 0.000 description 3
- 230000006835 compression Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 3
- 239000011859 microparticle Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- DXBHBZVCASKNBY-UHFFFAOYSA-N 1,2-Benz(a)anthracene Chemical compound C1=CC=C2C3=CC4=CC=CC=C4C=C3C=CC2=C1 DXBHBZVCASKNBY-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 2
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- BWLBGMIXKSTLSX-UHFFFAOYSA-N 2-hydroxyisobutyric acid Chemical compound CC(C)(O)C(O)=O BWLBGMIXKSTLSX-UHFFFAOYSA-N 0.000 description 1
- 241000276573 Cottidae Species 0.000 description 1
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 1
- 239000012494 Quartz wool Substances 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- -1 octadecylsiloxane Chemical class 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- VMXUWOKSQNHOCA-UKTHLTGXSA-N ranitidine Chemical compound [O-][N+](=O)\C=C(/NC)NCCSCC1=CC=C(CN(C)C)O1 VMXUWOKSQNHOCA-UKTHLTGXSA-N 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
- B01D15/206—Packing or coating
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/56—Packing methods or coating methods
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/56—Packing methods or coating methods
- G01N2030/562—Packing methods or coating methods packing
- G01N2030/565—Packing methods or coating methods packing slurry packing
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US43247082A | 1982-10-04 | 1982-10-04 | |
| US432470 | 1982-10-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5970963A true JPS5970963A (ja) | 1984-04-21 |
Family
ID=23716300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58154255A Pending JPS5970963A (ja) | 1982-10-04 | 1983-08-25 | 狭径微粒子カラムの充填方法及びカラム |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS5970963A (fr) |
| CA (1) | CA1226249A (fr) |
| DE (1) | DE3334902A1 (fr) |
| FR (1) | FR2534027B1 (fr) |
| GB (1) | GB2128099B (fr) |
| IT (1) | IT1172412B (fr) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62282262A (ja) * | 1985-12-11 | 1987-12-08 | リ− サイエンテイフイツク，インコ−ポレイテイド | 注型多孔質栓を有するクロマトグラフイ−カラム及びその製造法 |
| US8063237B2 (en) | 2008-05-15 | 2011-11-22 | Essilor International (Compagnie Generale D'optique) | Sulfur modified silanes for the elaboration of high refractive index materials |
| JP2017067780A (ja) * | 2015-10-01 | 2017-04-06 | ダイオネックス コーポレイション | クロマトグラフィーカラムの充填方法、高圧で使用するための充填されたクロマトグラフィーカラム、及びその使用 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09184830A (ja) * | 1995-12-28 | 1997-07-15 | Daicel Chem Ind Ltd | 充填剤充填装置、充填剤の充填方法および充填剤充填カラム集合体 |
| US6913679B1 (en) * | 1999-02-11 | 2005-07-05 | The Regents Of The University Of California | Apparatus and methods for high resolution separation of sample components on microfabricated channel devices |
| GB0212468D0 (en) * | 2002-05-30 | 2002-07-10 | Sev Trent Lab Ltd | Gas chromatography sample injection system |
| GB2506165B (en) * | 2012-09-24 | 2017-04-12 | Thermo Electron Mfg Ltd | Improvements in and relating to chromatography columns |
| US9897578B2 (en) | 2012-09-24 | 2018-02-20 | Thermo Electron Manufacturing Limited | Chromatography columns |
| GB2506166B (en) * | 2012-09-24 | 2014-12-17 | Thermo Electron Mfg Ltd | Improvements in and relating to chromatography columns |
| JP7289994B2 (ja) * | 2021-03-17 | 2023-06-12 | 株式会社ニッスイ | 精製の方法および精製された製品 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2128090A1 (en) * | 1971-06-05 | 1972-12-14 | Siemens Ag | Chromatography column prodn - by progressive depositon of bed material from withdrawing tube |
| US4175037A (en) * | 1978-04-10 | 1979-11-20 | Whatman Inc. | Process for packing chromatographic columns |
| US4375163A (en) * | 1981-01-08 | 1983-03-01 | Varian Associates, Inc. | Method and apparatus for on-column detection in liquid chromatography |
| JPS57168157A (en) * | 1981-02-12 | 1982-10-16 | Asahi Chem Ind Co Ltd | High performance liquid chromatography column and analysis method using the same |
-
1983
- 1983-08-05 GB GB08321160A patent/GB2128099B/en not_active Expired
- 1983-08-25 CA CA000435335A patent/CA1226249A/fr not_active Expired
- 1983-08-25 JP JP58154255A patent/JPS5970963A/ja active Pending
- 1983-09-27 DE DE19833334902 patent/DE3334902A1/de not_active Withdrawn
- 1983-10-04 IT IT23130/83A patent/IT1172412B/it active
- 1983-10-04 FR FR8315801A patent/FR2534027B1/fr not_active Expired
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62282262A (ja) * | 1985-12-11 | 1987-12-08 | リ− サイエンテイフイツク，インコ−ポレイテイド | 注型多孔質栓を有するクロマトグラフイ−カラム及びその製造法 |
| US8063237B2 (en) | 2008-05-15 | 2011-11-22 | Essilor International (Compagnie Generale D'optique) | Sulfur modified silanes for the elaboration of high refractive index materials |
| JP2017067780A (ja) * | 2015-10-01 | 2017-04-06 | ダイオネックス コーポレイション | クロマトグラフィーカラムの充填方法、高圧で使用するための充填されたクロマトグラフィーカラム、及びその使用 |
| US10254256B2 (en) | 2015-10-01 | 2019-04-09 | Thermo Hypersil-Keystone Llc | Method of packing chromatographic columns, packed chromatographic columns for use at high pressures and uses thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2128099B (en) | 1986-07-02 |
| IT1172412B (it) | 1987-06-18 |
| IT8323130A0 (it) | 1983-10-04 |
| GB2128099A (en) | 1984-04-26 |
| CA1226249A (fr) | 1987-09-01 |
| FR2534027B1 (fr) | 1986-06-13 |
| DE3334902A1 (de) | 1984-04-05 |
| GB8321160D0 (en) | 1983-09-07 |
| FR2534027A1 (fr) | 1984-04-06 |
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