JPS5948801B2 - organ perfusion device - Google Patents
organ perfusion deviceInfo
- Publication number
- JPS5948801B2 JPS5948801B2 JP51148533A JP14853376A JPS5948801B2 JP S5948801 B2 JPS5948801 B2 JP S5948801B2 JP 51148533 A JP51148533 A JP 51148533A JP 14853376 A JP14853376 A JP 14853376A JP S5948801 B2 JPS5948801 B2 JP S5948801B2
- Authority
- JP
- Japan
- Prior art keywords
- perfusion
- heart
- cannula
- perfusion device
- organ perfusion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000010412 perfusion Effects 0.000 title claims description 24
- 210000000056 organ Anatomy 0.000 title claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000012530 fluid Substances 0.000 description 15
- 239000011521 glass Substances 0.000 description 11
- 241001465754 Metazoa Species 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000000747 cardiac effect Effects 0.000 description 5
- 230000002262 irrigation Effects 0.000 description 5
- 238000003973 irrigation Methods 0.000 description 5
- 210000005240 left ventricle Anatomy 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 210000000709 aorta Anatomy 0.000 description 4
- 210000004351 coronary vessel Anatomy 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 210000005248 left atrial appendage Anatomy 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 241000282465 Canis Species 0.000 description 1
- 210000001765 aortic valve Anatomy 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000000750 endocrine system Anatomy 0.000 description 1
- 230000010247 heart contraction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 210000005246 left atrium Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Landscapes
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
【発明の詳細な説明】
本発明は臓器殊に心臓を用いる動物実験の為の装置に関
し、特に小動物の摘出心の潅流に適した臓器潅流装置に
関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an apparatus for animal experiments using organs, particularly hearts, and particularly to an organ perfusion apparatus suitable for perfusing isolated hearts of small animals.
摘出臓器の潅流法は臓器構成の細胞に損傷を与えること
なく、生体の神経系や内分泌系を隔離した状態で臓器の
代謝や機能を検討できる優れた方法である為、これを用
いて多くの研究がなされてきた。The perfusion method of removed organs is an excellent method for examining the metabolism and function of organs while isolating the nervous system and endocrine system of the living body without damaging the cells that make up the organ. Research has been done.
例えば、心潅流法については既に１９世紀ランゲンドル
フ（Ｌａｎｇｅｎｄｏｒ ｆ ｆ ）によって、摘出心
の潅流が行なわれ、以来いろいろな改良、工夫が加えら
れ現在に至っている。For example, regarding the heart perfusion method, Langendorf (19th century) already carried out perfusion of an isolated heart, and since then various improvements and devices have been added to the present day.
しかしながら、臓器潅流法は高度の熟練した手術手技を
必要とする為か、報告されている潅流装置自体もまだ実
験室的域を出ず、市販されるべき標準品も見当らない現
状である。However, perhaps because the organ perfusion method requires highly skilled surgical techniques, the perfusion devices themselves that have been reported are still in the laboratory, and there are currently no commercially available standard products.
すなわち、既報告のランゲンドルフ潅流装置はいずれも
一装置一標本の操作を意図したものである為、−実験に
多数検体を必要とする場合には、長時間を要するのみな
らず得られたデータの統計的処理に当っても各検体間の
実験条件の同一性が問題となってくる等の不都合が生じ
る。In other words, all of the Langendorff perfusion devices that have been reported are intended to operate on one device and one sample. Therefore, when an experiment requires a large number of samples, it not only takes a long time, but also reduces the amount of data obtained. Even in statistical processing, problems arise, such as the problem of the sameness of experimental conditions between samples.
また、例えば心潅流実験において、同時に心筋を傷害せ
ずに心収縮力や心電図を測定する適当な手段も見出され
ていない。Furthermore, no suitable means has been found for measuring cardiac contractile force or electrocardiogram without simultaneously damaging the myocardium in, for example, cardiac perfusion experiments.
本発明者らはこれらの事情に鑑み鋭意研究を重ね、数個
の小動物の心臓を一度に同一条件で潅流すると共に心収
縮力、心電図を同時に測定し得る潅流装置を作製し、本
発明を完成した。In view of these circumstances, the present inventors have conducted extensive research, and have completed the present invention by creating a perfusion device that can perfuse the hearts of several small animals at the same time under the same conditions and simultaneously measure cardiac contractile force and electrocardiograms. did.
次に図示した実施例に基づいて本発明の内容を更に詳細
に説明する。Next, the contents of the present invention will be explained in more detail based on the illustrated embodiments.
第１図は本発明装置本体を示す斜視図で１は基板、２．
２’は支柱である。FIG. 1 is a perspective view showing the main body of the apparatus of the present invention, in which 1 is a substrate, 2.
2' is a support.
３は保温箱５および操作室８を形成する底板で、保温箱
５の外周壁６の外側部分で支柱２，２′の中段に係止し
である。Reference numeral 3 denotes a bottom plate forming the heat-insulating box 5 and the operation chamber 8, and is fixed to the middle stage of the columns 2, 2' at the outer side of the outer peripheral wall 6 of the heat-insulating box 5.
４は保温箱５および操作室８を形成する上板で前記底板
３と同様に支柱２，２′に係止しである。Reference numeral 4 denotes an upper plate forming a heat insulating box 5 and an operation chamber 8, which, like the bottom plate 3, is secured to the pillars 2 and 2'.
該上板４には、操作室８を形成する部分に所望数（図で
は６個）の貫通孔を穿って栓９を装着してあり、これに
対応して底板３にも受液漏斗１０を装置しである。The top plate 4 has a desired number of through holes (six in the figure) in which the operation chamber 8 is formed, and plugs 9 are attached to the bottom plate 3. This is the equipment.
前記栓９には潅流液供給管１１、心収縮力測定用カニユ
ーレ１２および心電図電極１３ 、１３’およびアース
電極１３′を貫通装着しである。A perfusion fluid supply pipe 11, a cannula 12 for measuring cardiac contraction force, electrocardiogram electrodes 13, 13', and a ground electrode 13' are fitted through the plug 9.
潅流液供給管１１の上部には薬物注入口１４、潅流液流
入口への接続口１５、潅流液流入口１６およびガス排出
コック１７を有する潅流液供給びん１８が一体に形成さ
れ、支持枠１９に設けられた支持具１９′により固定さ
れている。A perfusate supply bottle 18 having a drug injection port 14 , a connection port 15 to the perfusate inlet 15 , a perfusate inlet 16 and a gas discharge cock 17 is integrally formed on the upper part of the perfusate supply pipe 11 , and a support frame 19 It is fixed by a support 19' provided at.
２０は底板３に蝶番２９等により開閉自在に取付けられ
た操作室８（コの字型の保温箱内周壁７と上板４および
底板３で形成される）の前扉で、心臓２３をセットした
後、閉じ係止具２１，２２゜２１’、２２’により係止
することにより操作室８内を略気密状態にして諸計測を
行うべく構成しである。20 is the front door of the operation chamber 8 (formed by the U-shaped inner circumferential wall 7 of the insulation box, the top plate 4, and the bottom plate 3), which is attached to the bottom plate 3 so as to be openable and closable with a hinge 29, etc., and the heart 23 is set therein. After that, by locking with the closing locking tools 21, 22, 21', 22', the inside of the operation chamber 8 is made into a substantially airtight state, and various measurements are performed.
２４は受液漏斗１０より滴下する液を受は排出するため
に置かれている排液槽である。Reference numeral 24 is a drain tank placed to drain the liquid dripping from the liquid receiving funnel 10.
場合によりこの排液槽に代えて潅流液を採取するための
試験管立てを設置して、試料を採取した後再び排出槽を
置換えられることはいうまでもない。Needless to say, if necessary, a test tube stand for collecting the perfusion fluid can be installed in place of the drain tank, and the drain tank can be replaced again after the sample has been collected.
第２図中２５は温水流入口、２６は温水流出口であり、
２７は潅流液供給びん１８内の潅流液温度の測定用温度
計であり、２８は操作室温を測定する為の温度計である
。In Figure 2, 25 is a hot water inlet, 26 is a hot water outlet,
27 is a thermometer for measuring the temperature of the perfusion fluid in the perfusion fluid supply bottle 18, and 28 is a thermometer for measuring the operating room temperature.
第３図中２９は底板３と前扉２０とを連結する蝶番であ
る。Reference numeral 29 in FIG. 3 is a hinge that connects the bottom plate 3 and the front door 20.
第４図は本発明装置に動物の心臓をセットした状態の説
明図であって２３は心臓であり、大動脈カニユーレ１１
′は大動脈３０の起始部まで挿入され絹糸で固定される
。FIG. 4 is an explanatory diagram of the animal heart set in the device of the present invention, where 23 is the heart, and the aortic cannula 11
' is inserted to the origin of the aorta 30 and fixed with silk thread.
心収縮力測定用ガラスカニユーレ１２は電極１３と共に
左心耳を切開した箇所より左心房を経由して左心室内に
挿入されて左心耳に固定され、電極１３は左心室内壁に
接触せしめられる。The glass cannula 12 for measuring cardiac contractile force is inserted into the left ventricle through the left atrium through an incision in the left atrial appendage together with an electrode 13, and is fixed to the left atrial appendage, and the electrode 13 is brought into contact with the wall of the left ventricle.
電極１３’、 １３“は犬動脈起始辺に接触されるよう
調整止具３１により大動脈カニユーレ１１′に係止され
る。The electrodes 13', 13'' are secured to the aortic cannula 11' by an adjustment stop 31 so as to be in contact with the origin of the canine artery.
このように構成された本発明装置を使用する場合を、第
５図に示すフローチャートに基づいて説明する。The use of the apparatus of the present invention configured as described above will be explained based on the flowchart shown in FIG.
潅流液供給びん１８に供給される潅流液は予め意図する
実験目的に合せて調整され、貯液槽・イ：に貯えられ、
酸素又は他のガスと二酸化炭素の混合ガスを通気される
。The perfusate supplied to the perfusate supply bottle 18 is adjusted in advance according to the intended experimental purpose, and is stored in a liquid storage tank.
A mixture of oxygen or other gases and carbon dioxide is vented.
次いで潅流液は加温装置・口の中を通って、使用する心
臓の由来する動物の体温に合せて一定温度に加温された
のち潅流ポンプハにより一定の流速を保ちつつ各潅流液
流入口１６および潅流液供給びん１８を通って心臓に達
する。Next, the perfusate passes through the warming device and the mouth, and is heated to a constant temperature according to the body temperature of the animal from which the heart is being used.Then, the perfusion liquid is passed through the perfusion liquid inlet 16 while maintaining a constant flow rate by the perfusion pump. and the perfusion fluid supply bottle 18 to reach the heart.
その際あらかじめコック１７を開放し潅流液供給びん１
８の心臓連結端を指先で閉じることによりポンプで駆動
された潅流液はガラス管１１内および接続口１５と潅流
液圧計ことを連結する管内を満し、潅流液供給びん１８
内に貯まったときコック１７を閉じガラス管１１の先端
を押えた指を離すと潅流液は潅流液供給びん１８内の一
定水位を保ちつつガラス管および大動脈に挿入されたガ
ラスカニユーレ１１′を通って心臓の冠状動脈内に入る
。At that time, open the cock 17 in advance and fill the irrigation fluid supply bottle 1.
By closing the heart connection end of 8 with a fingertip, the perfusion fluid driven by the pump fills the glass tube 11 and the pipe connecting the connection port 15 and the perfusion fluid pressure gauge, and the perfusion fluid supply bottle 18 is filled.
When the fluid has accumulated in the fluid supply bottle 18, when the cock 17 is closed and the finger holding the tip of the glass tube 11 is released, the irrigation fluid flows through the glass tube and the glass cannula 11' inserted into the aorta while maintaining a constant water level in the irrigation fluid supply bottle 18. and enters the coronary arteries of the heart.
ガラスカニユーレ１１′の先端は大動脈弁を傷つけない
ように注意深く動物の大動脈３０内の左右の冠状動脈口
の直前まで挿入され、絹糸等により固定される。The tip of the glass cannula 11' is carefully inserted into the animal's aorta 30 just before the left and right coronary artery ostia so as not to damage the aortic valve, and fixed with silk thread or the like.
このようにすることにより潅流液は冠状血管のみ潅流す
るようになる。By doing so, the perfusion fluid perfuses only the coronary vessels.
このように心臓をガラスカニユーレ１１′を通じて潅流
系路に設置したのち左心耳に小さな穴をあけここより電
極１３と共に特殊ガラスカニユーレ１２を左心室まで挿
入する。After the heart is placed in the perfusion system through the glass cannula 11', a small hole is made in the left atrial appendage and the special glass cannula 12 and electrode 13 are inserted through the hole into the left ventricle.
このガラスカニユーレの先端は丸いふくらみをもち、し
かもそこには数個の小穴がついている。The tip of this glass canyule has a round bulge with several small holes attached to it.
このガラスカニユーレ１２の先端にゴム薄片３２をかぶ
せカニユーレ１２および液圧計ホにつづく閉鎖回路を水
で満たすことにより、左心室の内圧の変化を正確に測定
することができる。By covering the tip of the glass cannula 12 with a thin rubber piece 32 and filling the closed circuit connected to the cannula 12 and the hydraulic pressure gauge E with water, changes in the internal pressure of the left ventricle can be accurately measured.
この内圧の変化は心臓の収縮力として代表される。This change in internal pressure is represented by the contractile force of the heart.
一方、大動脈３０内に電極１３’、 １３“（銀線を組
織に軽くふれておくのみ）を設置し、左心室内に設置し
た電極（銀線）より心腔内心電図を誘導することができ
る。On the other hand, electrodes 13', 13'' (simply touching the tissue with silver wires) are placed inside the aorta 30, and an intracardiac electrocardiogram can be induced from the electrodes (silver wires) placed inside the left ventricle. .
この方法は心臓の動きにつれて電極がずれたりはずれた
りすることがなく又組織を損傷することが少ないので従
来の方法と比較して非常に精度の高い測定データを得る
ことができる。With this method, the electrodes do not shift or come off as the heart moves, and there is less damage to tissue, so it is possible to obtain measurement data with much higher accuracy than with conventional methods.
その他第５図中３３はガスボンベ、３４はヒーター、３
５は電極１３゜１３’、 １３“に接続した増巾器、３
６は記録計、３Ｔは温水循環ポンプ、３８は温水槽であ
る。In addition, 33 in Figure 5 is a gas cylinder, 34 is a heater, 3
5 is an amplifier connected to electrodes 13° 13', 13", 3
6 is a recorder, 3T is a hot water circulation pump, and 38 is a hot water tank.
而して本発明では斜上の如き構成及び作用を有するもの
であるから複数個の検体を一度に同一条件下で潅流を行
い且つ心収縮力や心電図の測定を行い得各検体の測定デ
ータの比較ができる等の効果がある。Since the present invention has a configuration and function similar to that of a diagonal top, it is possible to perfuse a plurality of specimens at once under the same conditions, and to measure cardiac contractile force and electrocardiograms. This has the effect of allowing comparisons to be made.
第１図は本発明装置の斜視図、第２図は本装置の要部の
一部縦断正面図、第３図は操作室の縦断側面図、第４図
は本発明装置に動物の心臓をセツトした状態の拡大図、
第５図は本発明装置を使用する際のフローチャートの概
略図である。
８・・・・・・操作室、９・・・・・・栓、１０・・・
・・・受液漏斗、１１・・・・・・潅流液供給管、１８
・・・・・・潅流液供給びん、１２・・・・・・ガラス
カニユーレ、１３・・・・・・電藤。Fig. 1 is a perspective view of the device of the present invention, Fig. 2 is a partially vertical front view of the main parts of the device, Fig. 3 is a longitudinal side view of the operation chamber, and Fig. 4 is a perspective view of the device of the present invention. Enlarged view of the set state,
FIG. 5 is a schematic diagram of a flowchart when using the apparatus of the present invention. 8...Operation room, 9...Bung, 10...
...Liquid receiving funnel, 11...Irrigation liquid supply pipe, 18
... Irrigation fluid supply bottle, 12 ... Glass cannula, 13 ... Dendo.
Claims (1)
、複数個の栓を装着すると共に該栓に対応して底板に複
数個の受液漏斗を配置する一方、前記各個の栓に潅流液
供給びんの潅流液供給管とカニユーレと電極１３． １
３’、 １３”とを挿通装着してなる臓器潅流装置。1 A plurality of stoppers are attached to a top plate fixed to a column forming a part of the operation room, and a plurality of liquid receiving funnels are arranged on the bottom plate corresponding to the stoppers, while each of the above-mentioned stoppers Attach the irrigant supply tube, cannula, and electrode of the irrigant supply bottle to 13. 1
An organ perfusion device made by inserting and attaching a 3' and 13".
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP51148533A JPS5948801B2 (en) | 1976-12-10 | 1976-12-10 | organ perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP51148533A JPS5948801B2 (en) | 1976-12-10 | 1976-12-10 | organ perfusion device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5372390A JPS5372390A (en) | 1978-06-27 |
| JPS5948801B2 true JPS5948801B2 (en) | 1984-11-29 |
Family
ID=15454896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP51148533A Expired JPS5948801B2 (en) | 1976-12-10 | 1976-12-10 | organ perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5948801B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6175922U (en) * | 1984-10-19 | 1986-05-22 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7963163B2 (en) * | 2005-10-14 | 2011-06-21 | Johnson & Johnson Vision Care, Inc. | Systems and methods for detecting fluids |
-
1976
- 1976-12-10 JP JP51148533A patent/JPS5948801B2/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6175922U (en) * | 1984-10-19 | 1986-05-22 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5372390A (en) | 1978-06-27 |
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