JPS5948678B2 - Food factory wastewater treatment method using symbiotic microorganisms - Google Patents

Description

Detailed Description of the Invention The present invention relates to a food factory wastewater treatment method using symbiotic microorganisms, and more specifically, the present invention relates to a food factory wastewater treatment method using symbiotic microorganisms. Due to the synergistic effect of B, 0. The present invention relates to a food factory wastewater treatment method that can efficiently purify food factory wastewater with a high D value and harvest by-product microorganisms that can be effectively used as feed, fertilizer, etc.

In general, it is well known that photosynthetic bacteria, such as photosynthetic bacteria belonging to the genus Rhodopseudomonas, Rhodospirillum, and Chromatum, can be used to purify organic wastewater, and the present inventors have already utilized the above-mentioned photosynthetic bacteria. , Purification of night soil (Special Publication No. 45-12631), Purification of wool washing waste fluid (Special Publication No. 28234, Showa 45), Purification of starch waste fluid (Special Publication No. 18670, Showa 46), Purification of hydrocarbon fermentation waste fluid C Special Publication No. 1977 -27668), etc.

The present inventors also utilized photosynthetic bacterial cells produced as a by-product during wastewater treatment as feed (Japanese Patent Publication No. 47-1677
No. 2), used as fertilizer (Special Publication No. 45-14091)
It has also been successfully put into practical use.

The present inventor has conducted research on organic wastewater treatment using photosynthetic bacteria, and has established a symbiotic relationship with photosynthetic bacteria in the target organic wastewater when purifying organic wastewater using photosynthetic bacteria. We discovered the shocking fact that when we use a specific bacterial strain that exhibits Food factory wastewater in Japan, especially wastewater containing large amounts of starch (e.g. starch factory wastewater), protein, and nucleic acids.

Water (e.g. tofu, miso, dairy products, fish, beef packaging factory wastewater, slaughterhouse wastewater), wastewater containing large amounts of fat (e.g. fish, beef packaging factory wastewater, oil extraction factory wastewater), pectin, This is noticeable when wastewater containing a large amount of cellulose (e.g. wastewater from a fruit packing factory) or fermentation wastewater containing alcohol, vinegar, soy sauce, etc. (e.g. fermentation factory wastewater) is used. The present invention was completed after confirming that the plant has excellent performance as feed and fertilizer.

That is, the present invention provides for the production of photosynthetic bacterial cells selected from the group of photosynthetic bacteria belonging to the genus Rhodochium, Rhodospirillum, or Chromatum in food factory wastewater, and the photosynthetic bacterial cells and the target food. Purification is achieved by cultivating a mixture of the following specific bacterial strains that exhibit a symbiotic relationship in factory wastewater.

This is a food factory wastewater treatment method using symbiotic microorganisms that involves chemical treatment.

Next, the configuration and effects of the method of the present invention will be explained.

First, the photosynthetic bacterial cells and specific bacterial strains used in the method of the present invention will be described.

As the photosynthetic bacterium, one belonging to the genus Rhodopseudomonas, Rhodospirillum, or Chromatum is used.

For example, Rhodopseudomonas capsulatus (Rho)
dopseud-monascapsu 1atus
: Microtechnical Research Bulletin No. 879).

Rhodopseudomonas sulpheroides (Rhodo-p.
seudomonasshhero 1des:
Fermentation Research Institute strain No. 12203), Rhodospirillum rubrum
rum: Ohroma-tium vinosum No. 878, and Chromatium vinosum (Feikoken No. 890).

Specific bacterial strains include lactic acid bacteria (Lactobacillus
s: Fermentation Research Institute strain No. 3205, No. 3532, No. 355
No. 3, No. 3809, No. 3961, No. 3425),
Alcohol yeast (Hakkoken strain No. 2090), beer yeast (Hakkoken strain No. 2017), baker's yeast (Hakkoken strain No. 2046, 2044), sake yeast (Hakkoken strain No. 2164), wine yeast (Fukakken Bacterial Strain No. 2363)
, Ash-bya: Fermentation Research Institute Strain No. 056
No. 0, No. 1355), Candida (Oandida:
Fermentation Research Institute strain No. 0566, No. 0691, No. 1086
No., No. 0717, No. 0617, No. 0837), Brettano-myces (Fukkoken strain No. 0627, No. 0628), Oryptococcus: Ferkoken strain No. 037
No. 7, No. 0378, No. 0612, No. 1193, No. 0411, No. 0943, No. 1420), Debaryn-myces (Hakkoken strain No. 0015, No. 0855), Endomycobsis (E
ndomy cops is: Fermentation Research Institute Strain No. 0101
No., No. 0844, No. 0672), Hansenula (H
ansenula: Hakkoken strain No. 0140, No. 01
48), crockera (K1oekera: Hakkoken strain No. 0868), pihya (P1chia: Hakkoken strain No. 0193), Rho-doto
rula: Hakkoken Bacterial Strain No. 1541, No. 1433),
Saccharomyces:
Hakkoken strain No. 0849, No. 0504, No. 0293, No. 0525, No. 0021), Schizosaccharomyces (Hakkoken strain No. 0345), Torulopsis (Torulo-
psi: Ferkoken Bacterial Strain No. 0861), Bacillus (Ba
cillus: Fermentation Research Institute strain No. 3020, No. 3968
No., No. 3514, No. 3001, No. 3951), Streptococcus (Hakkoken Strain No. 12007), Staphylococcus (Sta.
-phy 1ococcus: Fermentation Research Institute strain No. 3060
), Psedomonas (Hakkoken strain No. 3445, No. 3458), Aspergillus (Hakkoken strain No. 428)
No. 1, No. 6086, No. 4075, No. 4091),
Rhizopus: Fermentation Research Institute Strain No. 4745
No. 4697), 'y Louis Vellomyces (Klu
-veromyces: Fermentation Research Institute strain No. 0433),
Pleurotus: Fermentation Research Institute Strain No. 6
No. 515), Kuehneromyces
yces: Fermentation Research Institute Strain No. 6141), Flammulina (
Fla-mmulina: Hakkoken strain No. 4901),
Acetobacter (Hakkoken strain No. 3281), Streptomyces (S.
tomyces: Hakkoken strain No. 3359), Nocardia (Hakkoken strain No. 33)
No. 85 is mentioned.

All of the above-mentioned specific strains exhibit a symbiotic relationship with the photosynthetic bacterial cells in food factory wastewater, and exhibit a remarkable purifying effect.

In addition, based on the results of extensive experiments over many years, the present inventors have selected an appropriate strain from the above-mentioned specific strains according to the type of food factory wastewater, and when performing mixed culture with the photosynthetic bacteria, the It has been confirmed that purification efficiency can be achieved.

Examples of appropriate combinations are as follows.

A: For wastewater containing large amounts of starch, lactic acid bacteria,
Alcohol yeast, beer yeast, baker's yeast, sake yeast, wine yeast, Hansenula, Satucharomyces, Schizosatucharomyces, Torulopsis.

B For wastewater containing large amounts of proteins and nucleic acids, use lactic acid bacteria and Candida.

Pseudomonas and Aspergillus for wastewater containing a large amount of C fat.

D For wastewater containing large amounts of pectin and cellulose, use Aspergillus, Satucharomyces, and Flammulina.

E For fermentation wastewater such as alcohol, vinegar, and soy sauce, Acetobacter, Streptomyces, and Nocardia.

Next, aspects of wastewater treatment will be described.

For wastewater treatment, as in the case of wastewater treatment using well-known photosynthetic bacterial cells, a treatment tank (culture tank) is prepared,
The target food factory wastewater may be introduced into the wastewater, and the photosynthetic bacterial cells and the specific bacterial strain may be mixed and cultured in the wastewater.

Incidentally, a lighting device, a ventilation device, etc. may be installed in the processing tank as necessary, and the installation may be performed using a well-known technique.

The photosynthetic bacterial cells and the specific bacterial strain to be mixedly cultured in wastewater may be seed-cultured separately in advance, and in this case, the photosynthetic bacterial cells and the specific bacterial strain are usually cultured separately. Purification can be achieved by inoculating about 0.01% of the wastewater to be treated by using it at a ratio of 1:1.

Of course, the mixing ratio and usage amount of the photosynthetic bacterial cells and the specific bacterial strain can be appropriately selected depending on the type of bacteria, the type of wastewater, the use of the harvested bacterial cells, etc.

Furthermore, instead of seed culture, it is also possible to use a portion of the wastewater to be treated in which the photosynthetic bacterial cells and the specific bacterial strain have been mixedly cultured, and in this case, the wastewater to be treated where the bacteria are growing All you have to do is collect a portion and put it into another treatment tank.

The conditions for wastewater treatment may be under light irradiation or darkness, and may be under aerobic or anaerobic conditions, but the amount of dissolved oxygen in the wastewater to be treated should be within the range of 0.1 to 6 ppI [l]. This is preferable for maintaining a good symbiotic relationship.

The treatment time varies depending on the type of bacteria used and the type of wastewater to be treated, but if the bacteria growing in the wastewater to be treated are harvested within 8 to 24 hours after starting mixed culture,
Purification is complete.

Incidentally, the bacteria may be harvested by well-known means.

When treating wastewater in the manner described above, some of the specific bacterial strains mentioned above have a high purification activity when there is a fixed bed, such as Aspergillus and Rhizopus, so these strains are selected. When used, it is desirable to install a well-known fixed bed or rotary mouth bed in the treatment tank.

According to the method of the present invention described above, B, 0. Food factory wastewater with high D value, especially B, 0. It can purify concentrated wastewater with a D value of several thousand pp [Il] to a few phlegm in a highly efficient 8 to 24 hours, and also has the advantage that the bacterial cells produced as a by-product during treatment can be effectively used as feed and fertilizer. It is.

Further, wastewater containing large amounts of pectin and cellulose, such as wastewater from a fruit canning factory, is difficult to purify using the well-known activated sludge method, but the method of the present invention can also be applied to such wastewater.

Furthermore, wastewater from alcoholic fermentation, which uses blackstrap molasses as a raw material, which is a type of fermentation industrial wastewater, not only has high B, D, and D values, but also has a brown color, and it is extremely difficult to make it transparent. Although it is said to be difficult, if the method of the present invention is applied, B, 0. In addition to lowering the directivity of the doctor, it can also be made transparent.

Next, the configuration and effects of the method of the present invention will be explained using examples.

Example 1゜ Contains a large amount of starch, B, 0. Starch factory wastewater with a D value of 5 sooppm was targeted, and Rhodopseudomonas pusillatus (Feikoken Bibori No. 879) was detected as a photosynthetic bacterial cell.
The following treatment was carried out using lactic acid bacteria (Hakkoken Bacteria Strain No. 3205) as a strain that exhibits a symbiotic relationship with this.

First, the starch factory wastewater was introduced into a treatment tank, and the Rhodopseudomonas capsulatus and the lactic acid bacteria, each of which had been seed-cultured in advance, were added at a ratio of 1:1 to 0.01% of the total amount of wastewater. % (wet bacteria amount) and mixed culture was carried out in a state where the amount of dissolved oxygen (D, 0) in the wastewater was maintained at 0.1 to 1.

After 12 hours, the bacteria in the wastewater were collected, and the number of bacteria was 5. fl (dry type) can be harvested, and B, 0. The D value had fallen to 35 pIXn.

For comparison, in the case of the above-mentioned Rhodopseudomonas pusulatus alone and the above-mentioned case of lactic acid bacteria alone, the conditions were exactly the same as above, and the amount added was 0.0% to the total amount of wastewater.
It was addressed to 01%.

- In the former case, the yield of bacterial cells was 0.3 g per 11 wastewater, B, 0. D value is 680pfl
In the latter case, the yield of bacterial cells is 11% of the wastewater.
The B and OD values fell to sooppm.

Examples 2-19 Example 1. Example 1 was carried out under exactly the same conditions as in Example 1, except that the lactic acid bacteria in Example 1 was changed to various strains and the treatment time was also changed depending on the strain used. The same wastewater was treated.

The results are shown in Table 1 below.

The same table also shows the results when various strains were used alone for comparison.

In Table 1, the upper row shows the results when the mixture was cultured with Rhodopseudomonas pusulatus (Keiko Bacteria No. 879), and the lower row shows the results when it was used alone.

Example 20 Contains a large amount of protein, B, 0. D value 3800pp[[l
We used Rhodopseudomonas schuphaeroides (Fakkoken strain No. 3205) as the photosynthetic bacterial cell and Bacillus (Fakkoken strain No. 3020) as the bacterial strain that exhibits a symbiotic relationship with the photosynthetic bacteria. Then, the following processing was performed.

First, the above milk product factory wastewater is introduced into a treatment tank, and the above Rhodopseudomonas chefheroides and the above Bacillus, which have been seed cultured in advance, are added to the tank at a ratio of 1=1 to 0% of the total amount of wastewater. Added at a volume ratio of .01% (wet bacteria amount), dissolved oxygen amount (D, 0) in wastewater 1° oppm
A mixed culture was carried out while maintaining the condition.

After 12 hours, the bacteria in the wastewater were collected, and 4.0g (dry type) of bacteria could be harvested per 11 liters of wastewater. The D value had dropped by 401)X.

For comparison, in the case of Rhodopseudomonas schuphaeroides alone and the case of Bacillus alone, the conditions were exactly the same as above - the amount added was 0.0 for the total amount of wastewater, respectively.
1%.

- In the former case, the yield of bacterial cells was 0.2 F, B, 0.2 F/14 wastewater. The D value has decreased to 75 Qppm, and in the latter case, the bacterial yield is 0.2 per 11 wastewaters. ? , B, 0. The D value fell to 9001)I)m.

Examples 21 to 25 The same waste water as in Example 20 was used under exactly the same conditions as in Example 20, except that the Bacillus in Example 20 was changed to various strains and the treatment time was also changed depending on the strain used. It was processed as follows.

The results are shown in Table 2 below.

The same table also shows the results when various strains were used alone for comparison.

In Table 2, the upper row shows the results when mixed culture with B:4 Pseudomonas schuphaeroides (Hakkoken strain No. 3205), and the lower row shows the results when it was used alone.

Example 26 Contains a large amount of oil and fat, B, 0. D value 9400 pI)! If
The wastewater from a fish and meat processing factory exhibiting
The following treatments were carried out using Aspergillus (Hakkoken strain No. 4281) as a strain that exhibits a symbiotic relationship with Aspergillus.

First, the above fish meat processing product factory wastewater is introduced into a treatment tank, and the above Chromatum binosum and the above Aspergillus, which have been seed cultured in advance in this N, are added at a ratio of 1:1 to 0% of the total amount of wastewater. It was added at a volume ratio of .01% (wet bacteria amount), and mixed culture was carried out while maintaining the amount of dissolved oxygen in the wastewater (D, 0) at 3.0 ppm.

After 24 hours, the bacteria in the wastewater were collected, and 1 (1 (dry) bacteria) could be harvested per 11 wastewater, and the B, 0.D value of the treated water after harvesting the bacteria was 501) INn? It was going down.

For comparison, the same conditions as above were used for the case of Chromatum vinosum alone and the case of Aspergillus alone, but the amounts added were each 0.01% based on the total amount of wastewater.

- In the former case, the bacterial yield was 0.8 g per 11 wastewater, and the B, O, and D values were 800 fermentations, and in the latter case, the bacterial yield was 0.8 g per 11 wastewater. 0 per 11.
7g, B, 0. The D value dropped to 1,000.

Examples 27 to 29 Example 26 except that the Aspergillus in Example 26 was replaced with various strains and the treatment time was also changed depending on the strain used.
The same wastewater as in Example 26 was treated under exactly the same conditions as in Example 26.

The results are shown in Table 3 below.

In addition, the same table also shows the results when various bacterial strains were used alone for comparison.

In Table 3, the upper row shows the results when the mixture was cultured with Chromatum binosum (Feikoken Binosum No. 890), and the lower row shows the results when it was used alone.

Example 3: Containing a large amount of pectin, B, 0. D value 4600ppIl
The target was wastewater from a mandarin cannery factory exhibiting a
The following treatments were carried out using Aspergillus (Hakkoken strain No. 4075) as a strain that exhibits a symbiotic relationship with Aspergillus.

First, the waste water from the tangerine canning factory was introduced into a treatment tank equipped with a rotary spout, and the Rhodospirillum rubrum and Aspergillus species, each of which had been seed cultured in advance, were introduced into the tank in a 1:1 ratio. It is added at a volume ratio of 0.01% (wet bacteria amount) to the total amount of wastewater, and the dissolved oxygen amount (D, 0) in the wastewater is
Mixed culture was carried out with the concentration maintained at 3.5 ppm.

After 48 hours, the bacteria in the wastewater were collected, and 6.0g (dry type) of bacteria could be harvested per 11 wastewaters. The D value had fallen to 20 ppm.

For comparison, in the case of Rhodospirillum rubrum alone and the case of Aspergillus alone, conditions were exactly the same as above, and the amounts added were each 0.01% based on the total amount of wastewater.

- In the former case, the yield of bacterial cells was 0.1 g/14 wastewater, B, 0. The D value is 30,001), and in the latter case, the bacterial yield is 0.5.9 per 11 wastewater, B, 0. D value is 7
001) I) It went down to III.

Examples 31 to 35 Example 30 except that the Aspergillus in Example 30 was replaced with various strains and the treatment time was also changed depending on the strain used.
The same wastewater as in Example 30 was treated under exactly the same conditions as in Example 30.

The results are shown in Table 4 below.

The same table also shows the results when various strains were used alone for comparison.

In Table 4, the upper row shows the results when mixed culture with Rhodospirillum rubrum (Feikoken Bacteria No. 878), and the lower row shows the results when it was used alone.

Example 36 B-OD of alcohol made from blackstrap molasses with fermentation wastewater
The target species were those exhibiting a value of 4000111) In and a brown color (colorimetric value of 350M, 0.95), using Rhodopseudomonas capsulatus (Feikoken Bacteria No. 879) as the photosynthetic bacterial cell. Using Candida (Hakkoken strain no. 0617) as a strain that exhibits a symbiotic relationship with
The following processing was performed.

First, the above wastewater was introduced into a treatment tank equipped with a rotary spout bed, and in step 5, the above rhodopseudomonas capsulatus and the above Candida, which had been cultured in advance, were added to the entire wastewater at a ratio of 1:1. Added at a volume ratio of 0.01% (wet bacteria amount) to the amount of dissolved oxygen in wastewater (D,0) 2.0p
Mixed culture was performed while maintaining the temperature at pm.

After 24 hours, the bacteria in the wastewater were collected, and 8.0g (dry form) of bacteria could be harvested per 11 ounces of wastewater, and the B, O, and D values of the treated water after harvesting the bacteria had fallen to 40 ppIn. .

Furthermore, the colorimetric value of the treated water was approximately 0.08 at a wavelength of 350M, indicating that the water was transparent.

For comparison, in the case of the above Rhodopseudomonas pushulatus alone and the above case of Candida alone, the conditions were exactly the same as above - the amount added was 0.01 for each of the total amount of wastewater.
%.

- In the former case, the yield of bacterial cells was 0.3 g per 11 wastewater, B, 0. The D value is 450p, and the colorimetric value of the treated water is about 0.85 at a wavelength of 350p, and in the latter case, the bacterial yield is 0 per 11 wastewater.
．． The BO, D value was 600p downstream, and the colorimetric value of the treated water was about 0.80 at a wavelength of 350M.

Examples 37-41 The same wastewater as in Example 36 was treated under exactly the same conditions as in Example 36, except that Candida in Example 36 was replaced with various strains and the treatment time was also changed depending on the strain used. I did it.

The results are shown in Table 5 below.

The same table also shows the results when various strains were used alone for comparison.

In Table 5, the upper row shows the results when the mixture was cultured with Rhodopseudomonas capsulatus (Feikoken Bacteria No. 879), and the lower row shows the results when it was used alone.

Regarding the colorimetric value of the treated water, the same results as in Example 35 were obtained.

Claims (1)

[Scope of Claims] 1. In food factory wastewater, Rhodopseudomonas
s), genus Rhodospirillum (Rhodospirillum)
I l Ium) genus, Chromatium
Photosynthetic bacterial cells selected from the group of photosynthetic bacteria belonging to the genus Ium) and lactic acid bacteria.
1 us), Arco → Shi yeast, Bi → Shi yeast, Baker's yeast,
Sake yeast, wine yeast, Ashbya, Candida, Brettanomyces (B r
et tanomyces), Cryptococcus (
C! ryptococcus), Thevariomyces (De
Baryomyces), Endomycobsis (End)
nmyccepsis), Hansenula (Hanse
-nula), Crockera (K 1oekera),
Pichia, Rhodoto
rula), Satucharomyces (5accharo, m
yces), Schizosaccharomyces
accharomyces), torulopsis (Toru
lopsis), Bacillus (Bacillus),
Streptococcus (Streptococcus)
s) Staphylococcus (S taphy 1oco
ccus), Pseudomonas (Pseudomonas)
n a s ), Aspergillus ( Asper gi
Rh 1us), Rhizopus, Kluyveromyces
), Ple+] lotus, Kuehneromyces,
Flammu-1ina, Acetobacter, Streptomyces, Nocardia (N
Food factory wastewater treatment by utilizing symbiotic microorganisms, characterized in that purification treatment is performed by mixed culturing of the photosynthetic bacterial cells and a strain exhibiting a symbiotic relationship in target food factory wastewater selected from P. ocardia. Law.