JPH07132081A - Cell composition - Google Patents

Abstract

PURPOSE:To obtain a cell composition useful as a simple cleaning agent for water qualities, suitable for preservation in frozen state, comprising a cell of an active photosynthetic bacterium and water as constituent materials. CONSTITUTION:This cell composition is obtained by diluting (A) a cell of photosynthetic bacterium belonging to the family Athiorhodaceae, Thiorhodaceae or Chloro-bacteriaceae, etc., with (B) water. When the cell composition is thrown into water, the cell takes nitrogen and phosphorus as a feed, is further activated if conditions of light and temperature are proper to suppress inclination of eutrophication of a closed water system in which fishes are laid. The cell is useful for cleaning water and maintaining water qualities.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a bacterial cell composition, and more particularly to a bacterial cell composition which functions as a water purification agent useful for application to a closed water system (for example, a water tank or a pond).

[0002]

2. Description of the Related Art Conventionally, in animals living in closed water systems, especially in fish,
The effectiveness of photosynthetic bacteria based on their nutritional value / nutritional effect and water purification effect is drawing attention.

That is, the photosynthetic bacteria are the Berjay's Manual of Determinative Bacter.
7th Edition), it belongs to the family of bacteria shown in Table 1 below, and has a size of 0.5 to 15μ and a growth pH of about 7.8 to about 8.5 as common morphology and properties. There is something.

[0004]

[Table 1]

However, in order to maintain the active state of the photosynthetic bacteria in the conventional diluted photosynthetic bacteria, in order to dilute the concentrated bacterial cell fluid to a predetermined amount, all the substrate components (nutrient salts) such as ammonium and phosphoric acid should be added. It was necessary to add about 20% by volume of the substrate. Therefore, mixing the concentrated bacterial cell fluid with the liquid containing these substrates was the general preparation method as a water purification agent.

For this reason, in the above-mentioned water purification agent containing photosynthetic bacteria, it is necessary to measure and dilute so as to obtain a predetermined substrate concentration. Therefore, the dose of cells depends on the dose of substrate. In addition, the handling operation was inconvenient and lacked in convenience, and it was also necessary to stir so that the substrate component and the bacterial cells were uniform.

Further, in the liquid water purification agent prepared by mixing the substrate component and the bacterial cells, for example, when the unused water purification agent is returned to the refrigerator (re-refrigerated), the bacteria of the water purification agent are accompanied by a change in temperature conditions. There is an aspect that it cannot be stored for a long period of time, such as partial dying or the generation of a bad odor due to the alteration of the substrate constituting the water purification agent.

Further, in the conventional dilute photosynthetic bacteria, as described above, due to the presence of the substrate component, when used in a large amount in a closed water area such as a pond or an aquarium, a primary product using the substrate as a nutrient source. It may promote the growth of phytospecific plankton (algae) that is, therefore, taking into consideration not only the growth of photosynthetic bacteria but also the plankton generation due to the input of the substrate, the input amount of the photosynthetic bacteria at the time of input,
The input period (interval), etc. must be set, and the actual situation requires some skill in using it.
If this adjustment is incorrect, it will result in an environment unfavorable for the growth of fish, such as polluting the water quality.

[0009]

SUMMARY OF THE INVENTION In view of the problems pointed out in the above-mentioned prior art, the present invention makes active bacterial cells of photosynthetic bacteria a constituent material of a water purification agent without depending on the substrate component. In order to provide a water purification agent, the inventors of the present invention have conducted extensive studies, and as a result, the present invention has been made. The gist of the invention is that it does not contain a substrate component and is suitable for frozen storage, and It is a bacterial cell composition that functions as a water purification agent containing photosynthetic bacteria and water, which provides convenience such as saving the labor of measurement.

The constitution of the bacterial cell composition of the present invention will be specifically described below.

In the present invention, as described above, the photosynthetic bacterium is used as the bacterial cell component. Basically, if the photosynthetic bacterium belonging to the family of bacteria listed in Table 1 above is included in the composition of the present invention. Applicable. In addition, even if these photosynthetic bacteria are used in a state in which any two or more kinds are mixed, the activity thereof is not particularly hindered, and a mode like this is also applicable to the present invention. The medium for diluting the photosynthetic bacteria may be any medium that can maintain the bacteria without killing them, and for example, water (filtered water, tap water, distilled water, etc.) is applicable. Further, the density of the bacterial cells in the bacterial cell dilution medium is preferably adjusted by a single-use amount described later, in consideration of maintaining good asphyxia of the bacterial cells.

Furthermore, by individually containing and packaging unit doses of the bacterial cell composition of the present invention, the required minimum amount can be obtained without requiring special skill, with reference to the amount of water in the aquarium and the number of fish. It is possible to easily determine the limited amount of the bacterial cell composition input, thereby preventing the waste of the feed and the deterioration of the water quality due to the excess feed. The method for packaging the bacterial cell composition described above is not particularly limited as long as it can store a unit dose of the composition, but the composition of the present invention is solid because it is frozen and stored. From the viewpoint of maintaining the shape of the composition (preventing shape collapse), press-through package (PT
P.) Packaging is preferred.

[0013]

In other words, the active photosynthetic bacterium employed in the composition of the present invention, once administered in water, supplements with nitrogen and phosphorus, which cause water quality deterioration (eutrophication) in water, as food. If the conditions of light (10,000 lux) and temperature (25 to 35 ° C) are appropriate, the cells are further activated and exhibit a water purification function. Also, while maintaining activity,
Since the bacterial cells discharged into water absorb the nutrients contained in the excrement from the living body dispersed in water and the food residue for their growth, the inhibition of the eutrophication tendency of the closed water system where the fish is placed and the water quality. Can be purified and maintained.

[0014]

EXAMPLES Examples of the present invention will be described in more detail below.

Example 1 Preparation of cell composition First, cells (5 g) of photosynthetic bacteria (PSB) were added to water (250 m).
The bacterial cell composition of the present invention obtained by diluting l) was applied to the production line of a fully automatic blister packaging machine (WIDER-AII: Bucheon Machinery Co., Ltd.), and its unit dose (2 ml) was PT.
P. Each container was poured into a container, the container was covered with aluminum foil, and then rapidly frozen at -45 ° C.
(° C).

Example 2: Water purification effect and nutrient absorption of bacterial cell composition
Assay of yield effect The water purification effect and nutrient absorption effect of the bacterial cell composition of the present invention obtained in Example 1 were assayed according to the following procedure.

[Sample] Sample of the present invention …… The bacterial cell composition obtained in Example 1 Control sample …… Conventional diluted photosynthetic bacteria (PSB) [Dose] Sample of the present invention …… 13.5 ml [water and bacterial cells (Total amount of (0.27g)] Control sample …… 67.5ml [Total amount of substrate solution and bacterial cells (0.27g)] Frequency of administration: Only once (first time) on the test start day [Experimental condition] Collected from natural pond Two aquariums (3 liters) filled with water (pH 9.4), each containing 2 to 2-3 cm goldfish
Prepared). An aeration device was connected to each water tank, and the above-mentioned samples were individually charged into each water tank while supplying air. The water temperature at the beginning of the experiment was 25 ° C, and no temperature control was performed thereafter.

Experiments were carried out under the above conditions, and the change in water quality was verified by measuring the total nitrogen and total phosphorus and the transparency of each water tank 4 days after the test was started. The results are shown in Table 2 below.

[0019]

[Table 2]

As is clear from the above results, according to the composition of the present invention, the transparency is remarkably improved as compared with the other samples, and the residual amounts of total nitrogen and total phosphorus are remarkably suppressed, and It was confirmed that the nutritional tendency was significantly suppressed and that the effects of water purification and conservation could be improved. In each of the aquariums, the amount of total nitrogen and total phosphorus increased from the beginning of the experiment is due to the physiological action of the living body (goldfish).

[0021]

EFFECTS OF THE INVENTION According to the present invention, by activating active photosynthetic bacteria as a water purification agent and by adopting a suitable (PTP) packaging without depending on the substrate used for the growth of photosynthetic bacteria, it is wasteful. Therefore, it is possible to easily provide only a predetermined dose of active photosynthetic bacteria, which saves the operation and work that requires skill related to the concentration of the substrate, and also causes the bad odor due to the alteration of the substrate and the bacteria at the time of refrigeration. It has the effect of eliminating the management problem of the death of the.

Further, since no substrate is used, the growth of phytospecific plankton (algae) is suppressed, water quality can be purified, and the introduced cells can be provided to animals and plants in a fresh state, resulting in excellent growth.・ It is also possible to realize a nutritional environment.

Claims (3)

[Claims] 1. A cell composition comprising a preparation comprising photosynthetic bacteria and water. 2. The cell composition is held in a container comprising a container for individually accommodating a unit dose of the composition and a covering material for sealingly covering the container. The bacterial cell composition described in 1. 3. The photosynthetic bacterium is an acioloderche (A
thiorhodaceae), Shiorodache (Thiorhodaceae)
Family and Chloro-bacteriace
The bacterial cell composition according to claim 1 or 2, which is a bacterium belonging to one family selected from the group consisting of ae) families.