JPS5928442A - Thiamine preparation for pisciculture - Google Patents

Abstract

PURPOSE:To prepare a thiamine preparation for pisciculture, by coating thiamine or a thiamine salt with a non-oleaginous coating agent such as polyvinyl acetate, ethyl cellulose, etc. CONSTITUTION:The present coating agent is prepared by conventional method such as spray-coating, rolling coating, micro-encapsulation, etc. In the case of coating by spray-coating, a coating agent such as a copolymer of 2-methyl-5- vinylpyridine, methyl acrylate and methacrylic acid, diethylaminoacetate of polyvinyl acetal, cellulose acetate phthalate, casein, etc. is molten with heat, and mixed with powdery thiamine or a thiamine salt such as thiamine hydrochloride, nitrate, lauryl sulfate, etc., and the mixture is sprayed to obtain powdery preparation.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to coated preparations of thiamine or thiamine salts for fish farming.

Thiamine preparations added to live feed for anchovies, saury, mackerel, sand eel, etc. used for rearing and aquaculture of yellowtail, sea bream, etc., include thiamine or thiamin salts coated with 1 to 10 times the amount of higher fatty acids or glycerin fatty acid esters. Formulations containing grains with a meridional diameter of 100 to 1000 microns have been well received.

However, these preparations have the disadvantage that the coating effect is poor when the particle diameter is 100 μm or less or when the amount of coating agent is relatively small. The present inventors succeeded in improving this formulation by using a fatty acid ester type or phospholipid type surfactant, but they further studied a coated formulation using a non-oleaginous coating agent as a coating agent and developed the present invention. completed.

It is a thiamin preparation.

Thiamine salts include thiamine hydrochloride, nitrate, lauryl sulfate, thiocyanate, cetyl sulfate, 1,5
-Naphthalene disulfonate, 2,6-naphthalene disulfonate, 7-taline salt, etc.

As the non-oil-based coating agent, water-resistant natural products or synthetic polymers such as solid wax, beeswax, higher alcohol, shellac, polyvinyl acetate, polyvinyl butyral, ethyl cellulose, acetyl cellulose, etc. are also used, but 2-methyl- 5-vinylpyridine, copolymer of methyl acrylate and methacrylic acid (MPM), diethylaminoacetate of polyvinyl acetal (AE
A), cellulose acetate phthalate (CAP), zein, and the like are particularly preferred because they have excellent water resistance and disintegration in the bodies of cultured fish. These coating agents may be used alone or in combination.

The content of thiamine or thiamine salts in the preparation is 10-9
It is preferably about 0%, and the particle size is suitably about 100 to 1000 microns.

As the coating method, all known methods can be applied. For example, spray method, transfer method, fluidized bed method, distributed cooling method,
Examples include a granulation method such as a microcapsule method, and a coating method, and an appropriate surfactant can also be mixed with the coating agent. The powder of thiamine or thiamine salts preferably has a particle size of about 5 to 500 μC.

Add powdered thiamine or thiamine salts.

Next, a powder formulation is obtained by spraying this mixture into a room at 60° C. or lower using a spray device. Furthermore, when manufacturing by the transfer method, the coating agent is dissolved in a solvent. As the solvent, methanol, ethanol, ingropanol, acetone, carbon tetrachloride, etc., and mixtures thereof are used. This solution is then sprayed onto powdered thiamine or thiamine salts, and the solvent is removed to obtain a powdered preparation.

Example Thiamine nitrate was coated with various coating agents to produce formulations with different thiamine nitrate contents and particle sizes, and each was mixed with minced anchovies as thiamin at a ratio of 10 m9/1 [10 g,
After standing at 30°C for 4 hours and 8 hours, the remaining amount of thiamine was measured. The results are shown in Table 1, and the coating effect of the formulation of the present invention is clearly superior to that of known formulations.

Table 1 Example 1 Japanese Pharmacopoeia beeswax (melting point approximately 62°C) 120kII was heated and melted to 75°C, thiamine nitrate SD ky having a particle size of approximately 100μ was added, and the mixture was stirred with a homomixer. , using a rotating disc sprayer 2
When sprayed into a room at 8°C, a coated thiamine formulation with a particle size of 200-400μ is obtained. Thiamine nitrate content in this formulation is approximately 20%.

Example 2 10 kJ of thiamin nitrate passed through a 60 mesh sieve was placed in a rotating coating pan, containing 90% zein and 15% zein.
Spray with a solution of Impropatool. After a thin film is formed, hot air is blown into the film and the film is dried at approximately 60°C. When the solvent evaporates, the above-mentioned zein-impropanol solution is repeatedly sprayed until a film of about 60% is formed.
A coated thiamine preparation with a particle size of 400-600μ is obtained.

Example 6 10 kg of thiamin nitrate passed through a 60 mesh sieve was placed in a rotating coating pan and coated with commercially available MPlv! A solution of an ethanol-carbon tetrachloride mixture containing 20% of a coating agent (a mixture of 2-methyl-5-vinylpyridine, methyl methacrylate and vinyl acetate and polyethylene glycol containing 60% of methacrylic acid copolymer) is sprayed. Thereafter, the same operation as in Example 2 is continued until a film containing about 20% of the MPM coating agent is formed, thereby obtaining a coated thiamin preparation with a particle size of 400 to 600 μm.

Example 4 1Q kg of thiamine nitrate passed through a 30 mesh sieve is placed in a rotating coating pan and sprayed with a solution of methanol-acetone mixture containing 10% of commercially available thiamine (polyvinyl acetal diethylamine acetate). The following operation was carried out in the same manner as in Example 2 except that the evaporation temperature of the solvent was 45°C.
If the process is continued until a film of about 15% of AF and A is formed, a coated thiamine preparation with a particle size of 400 to 600 μm can be obtained.

Example 5 10 ky of thiamin nitrate passed through a 30 mesh sieve was placed in a rotating coding pan and an acetone solution containing 10% Japanese Pharmacopoeia cellulose acetate phthalate (CAP) and 2.5% polyoxyethylene sorbitan monooleate was added. Spray. The following operation was carried out in the same manner as in Example 4, and the CAP was approximately 60.
% of the film is completed, the particle size will be 400 to 600.
A coated thiamin preparation of μ is obtained.

Application example Young sea bream (average weight approximately 12.0g) immediately after being caught 2X2X
Seven plots were set up with 50 fish each placed in seven 1m-long cages. After raising these for two days without feeding them, I fed them minced anchovies.

Comparison area 1 has thiamine nitrate content 1 of beef tallow hydrogenated oil coating.
4% formulation, and Example 1 for Moto Akane Ming Wards 1 to 5, respectively.
After diluting the preparations of 5 to 1% with lactose, they were mixed in 100 g of minced anchovy so that the thiamin nitrate content was approximately 5 m9, and the mixture was administered. On the other hand, in the control group, about 5 ml of thiamine nitrate was diluted with lactose to make 0.9 ml, which was mixed with 100 g of minced meat and administered.

The feeding amount for each section above is 100 I for days 0 to 5, and 100 I for days 6 to 5.
15th day is 1509. 16th to 21st day is 200g. Table 2 shows the results of measuring the rate of increase in body weight on days 0, 10, and 20 after the start of bait casting, as well as the thiamin content (in muscle) immediately after fishing and 20 days after fishing.

From these results, it is known that the preparation of the present invention exhibits superior effects than known coated thiamine preparations, and significantly prevents and improves the growth delay of anchovies caused by continuous feeding in Thai aquaculture.

Table 2

Claims (1)

[Claims] A thiamine preparation for fish farming that is coated with thiamin or thiamin salts with a non-oily coating agent.