JPS59224566A - 腫瘍ゲンに関連する再配列ｄｎａ断片の検出方法 - Google Patents
腫瘍ゲンに関連する再配列ｄｎａ断片の検出方法Info
- Publication number
- JPS59224566A JPS59224566A JP59032717A JP3271784A JPS59224566A JP S59224566 A JPS59224566 A JP S59224566A JP 59032717 A JP59032717 A JP 59032717A JP 3271784 A JP3271784 A JP 3271784A JP S59224566 A JPS59224566 A JP S59224566A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- dna
- oncogens
- fragment
- dna fragments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012634 fragment Substances 0.000 title claims description 20
- 238000000034 method Methods 0.000 title claims description 7
- 239000000523 sample Substances 0.000 claims description 14
- 230000003211 malignant effect Effects 0.000 claims description 9
- 230000008707 rearrangement Effects 0.000 claims description 9
- 208000011691 Burkitt lymphomas Diseases 0.000 claims description 6
- 239000013612 plasmid Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000012472 biological sample Substances 0.000 claims description 2
- 230000008711 chromosomal rearrangement Effects 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 12
- 238000009396 hybridization Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 5
- 210000000349 chromosome Anatomy 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 230000005945 translocation Effects 0.000 description 4
- 208000034951 Genetic Translocation Diseases 0.000 description 3
- 101001070329 Geobacillus stearothermophilus 50S ribosomal protein L18 Proteins 0.000 description 3
- 101100326370 Mus musculus Bst1 gene Proteins 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 241000271566 Aves Species 0.000 description 2
- 208000032791 BCR-ABL1 positive chronic myelogenous leukemia Diseases 0.000 description 2
- 208000010833 Chronic myeloid leukaemia Diseases 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 2
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 2
- 208000033761 Myelogenous Chronic BCR-ABL Positive Leukemia Diseases 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 102000043276 Oncogene Human genes 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 108700024542 myc Genes Proteins 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 108091023043 Alu Element Proteins 0.000 description 1
- 101001030211 Homo sapiens Myc proto-oncogene protein Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 108700005091 Immunoglobulin Genes Proteins 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 101150039798 MYC gene Proteins 0.000 description 1
- 206010064912 Malignant transformation Diseases 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 208000007452 Plasmacytoma Diseases 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 102000053563 human MYC Human genes 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000036212 malign transformation Effects 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- QPUKANZXOGADOB-UHFFFAOYSA-N n-dodecyl-n-methylnitrous amide Chemical group CCCCCCCCCCCCN(C)N=O QPUKANZXOGADOB-UHFFFAOYSA-N 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- WEQHQGJDZLDFID-UHFFFAOYSA-J thorium(iv) chloride Chemical compound Cl[Th](Cl)(Cl)Cl WEQHQGJDZLDFID-UHFFFAOYSA-J 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Hospice & Palliative Care (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Oncology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US469797 | 1983-02-25 | ||
| US06/469,797 US4542096A (en) | 1983-02-25 | 1983-02-25 | Detecting rearranged human c-myc DNA fragments associated with oncogenes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS59224566A true JPS59224566A (ja) | 1984-12-17 |
Family
ID=23865095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59032717A Pending JPS59224566A (ja) | 1983-02-25 | 1984-02-24 | 腫瘍ゲンに関連する再配列ｄｎａ断片の検出方法 |
Country Status (6)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61162752A (ja) * | 1985-01-11 | 1986-07-23 | Sekisui Chem Co Ltd | Ｄｎａおよび／またはｒｎａの検出法 |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4681840A (en) * | 1984-01-18 | 1987-07-21 | The United States Of America As Represented By The Secretary Of Commerce | Deoxyribonucleic acid molecules useful as probes for detecting oncogenes incorporated into chromosomal DNA |
| US4857466A (en) * | 1984-09-28 | 1989-08-15 | The Board Of Regents Of The University Of Texas System | Probe for detection of specific human leukemias |
| IL76997A0 (en) * | 1984-11-14 | 1986-04-29 | Oncogene Science Inc | Probes and methods useful for detecting chromosomal translocation |
| US4892829A (en) * | 1986-04-22 | 1990-01-09 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Human plasma cell line having rearranged c-myc proto-oncogene |
| US5320941A (en) * | 1986-06-06 | 1994-06-14 | Dallan Young | DNA sequence encoding mas onhcogene, polypeptides encoded therefrom and diagnostic and other methods based therefrom |
| EP0318502A1 (en) * | 1986-08-13 | 1989-06-07 | The General Hospital Corporation | Detection marker for colonic lesions |
| IL84007A0 (en) * | 1986-10-08 | 1988-02-29 | Gen Hospital Corp | Recombinant dna molecules which contain a plant-derived genetic sequence for glyceraldehyde phosphate dehydrogenase and a method of producing plant glyceraldehyde phosphate dehydrogenase |
| DE3721400A1 (de) * | 1987-06-29 | 1989-01-12 | Viktor Dr Dr Med Balazs | Verfahren zur untersuchung einer azellularen biologischen fluessigkeit auf zellulare onkogen-dna bzw. deren fragmente |
| US5066792A (en) * | 1987-09-04 | 1991-11-19 | Board Of Regents University Of Texas | Gene probe for detection of specific human leukemias |
| WO1992006220A1 (en) * | 1990-10-05 | 1992-04-16 | President And Fellows Of Harvard College | Detection and isolation of ligands |
| IL107250A (en) * | 1993-10-12 | 2001-08-08 | Yeda Res & Dev | Dna molecules involved in programmed cell death, proteins encoded thereby and methods and compositions using said dna molecules and proteins |
| US6160106A (en) * | 1993-10-12 | 2000-12-12 | Yeda Research & Development Co., Ltd. | Tumor suppressor genes, proteins encoded thereby and use of said genes and proteins |
| DE19932890A1 (de) * | 1999-07-19 | 2001-02-01 | Deutsches Krebsforsch | DNA zum Nachweis von Veränderungen des Chromosoms 8 |
| EP3000900B1 (en) * | 2010-04-23 | 2018-02-28 | Genomic Vision | Detection of hpv dna rearrangements |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2492985A1 (fr) * | 1980-10-24 | 1982-04-30 | Pasteur Institut | Procede d'etude et de detection d'un gene susceptible d'etre transcrit en un arn messager determine et de la capacite des cellules ou d'une fraction cellulaire d'en assurer la transcription et application au diagnostic in vitro de maladies dues a des anomalies genetiques ou a des adn etrangers |
| US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
-
1983
- 1983-02-25 US US06/469,797 patent/US4542096A/en not_active Expired - Fee Related
-
1984
- 1984-02-21 GR GR73861A patent/GR81659B/el unknown
- 1984-02-21 CA CA000447937A patent/CA1214092A/en not_active Expired
- 1984-02-23 EP EP84301179A patent/EP0117727A3/en not_active Withdrawn
- 1984-02-24 JP JP59032717A patent/JPS59224566A/ja active Pending
- 1984-02-24 DK DK98584A patent/DK98584A/da not_active Application Discontinuation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61162752A (ja) * | 1985-01-11 | 1986-07-23 | Sekisui Chem Co Ltd | Ｄｎａおよび／またはｒｎａの検出法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0117727A2 (en) | 1984-09-05 |
| EP0117727A3 (en) | 1988-01-20 |
| US4542096A (en) | 1985-09-17 |
| CA1214092A (en) | 1986-11-18 |
| DK98584D0 (da) | 1984-02-24 |
| DK98584A (da) | 1984-08-26 |
| GR81659B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1984-12-12 |
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