JPS5920357A - Production of conversion product of gardenia pigment - Google Patents

Abstract

PURPOSE:To produce a conversion product of gardenia pigment (yellowish green to blue), by culturing a specified microorganism of genus Streptomyces in a medium contg. a gardenia pigment extract. CONSTITUTION:Streptomyces canescens (ISP5001) or Streptomyces chatanoogensis (ISP5001) is used. Crushed gardenia fruit is extracted with an aq. ethanol soln. The resultant gardenia pigment extract is added to a medium, pref. a liquid medium which is then inoculated with the above microorganism. The inoculated medium is aerated and stirred at 27-37 deg.C and at a pH of 5.5-8. When the medium assumes the desired color tone [the color tone continuously changes from yellow (the color of the gardenia pigment extract) to yellowish green, green and then blue], the medium is sterilized and the culture is stopped. Cells are then removed and the resultant clear soln. is concentrated to obtain conversion product of a gardenia pigment.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention involves treating a gardenia pigment extract with a microorganism belonging to the genus Streptomyces, thereby producing gardenia pigments that are different in color from the original gardenia pigment extract, such as yellow-green, green, or blue. The present invention relates to a method for producing a converted product.

Traditionally, gardenia pigment has been widely used as a coloring agent for foods and drinks as a natural yellow pigment with excellent stability such as light resistance and heat resistance. As a general property of this gardenia pigment, it has been known that green colonies can be obtained by culturing a type of Bacillus subtilis on a standard agar medium containing a gardenia pigment extract.

Utilizing such properties, a method has been proposed in which Bacillus subtilis is allowed to act on a gardenia pigment extract to produce a gardenia pigment conversion product having a color tone different from that of the gardenia pigment extract. (Japanese Patent Publication No. 52-13971) The present inventors focused on the action of Bacillus subtilis on gardenia pigment extracts, and conducted various studies on bacterial species that have the ability to produce gardenia pigment conversion products that differ in color tone from gardenia pigment extracts. As a result, microorganisms belonging to the genus Streptomyces, which have never been known to have such abilities, produce gardenia pigment conversion products that have a color tone different from that of gardenia pigment extracts, especially yellow-green, greenish, or blue colors. It was discovered that gardenia pigment conversion products were produced.

The gardenia pigment extract used in the present invention is preferably extracted with water or a mixed solvent of water-alcohol, particularly methanol and ethanol. In the case of extraction with water, the extract may be used as it is; in the case of extraction with a water-alcohol mixed solvent, it may be used either after concentration or after dilution with water.

As the microorganisms that can be used in the present invention, any microorganisms belonging to the genus Streptomyces can be used as appropriate.
myces canescens ISP 500
1) Streptomyces chatanoogensis (St
r, chattanogensis I S P 5
0 M Streftomyces michiganensis (Str.

michiganensis X S P 5015
) Streptomyces blastomyceticus (Str
, bl, astmyceticus I S P 50
29) Streptomyces pyridochromogenes (S
tr, virid Ochromogenes engineering 5P51
10) Streptomyces fuereus (S, tr.

felleus engineering 5p5130) Streptomyces chryseus (Str.

griseus engineering 5P5236) Nutrebutomyces flavotrichini (str.

flavotricini I SP 5152
) etc. were found to have a high production capacity for gardenia pigment conversion products.

However, the culture medium of the present invention can only be used as a normal microbial culture medium containing a carbon source, nitrogen source, and other nutrients necessary for growth that can be assimilated by microorganisms belonging to the genus Streptomyces, but a liquid medium can be used. It is better to use it. culture temperature,
The pH is only within the range where microorganisms belonging to the genus Streptomyces can grow, but it is usually 27'-37°, P
It is desirable to carry out stirring aeration culture under conditions of H5, 5-8.

After culturing under the above conditions, sterilization is performed when the desired color tone is obtained, and the cells are filtered to obtain a clear liquid. By concentrating it into an extract form or powdering it, a yellow-green, green or blue gardenia pigment conversion product can be easily obtained, thereby achieving the object of the present invention.

In the manufacturing process of the gardenia pigment conversion product of the present invention, the color tone of the culture solution changes continuously from the initial yellow of the gardenia pigment extract to yellow-green, green, and blue as the culture time progresses. By stopping the culture at the stage when the desired color tone is obtained, it is possible to optionally obtain yellow-green, green, or blue gardenia pigment conversion products.

The thus obtained yellow-green, green or blue gardenia pigment conversion product is useful for coloring foods, drinks, medicines, cosmetics, etc. as a water-soluble natural pigment with excellent stability such as light resistance and heat resistance.

The method of the present invention will be explained below with reference to Examples.

Example 1 500 f of gardenia fruit is crushed, 2 tons of 50% aqueous ethanol is added, and the mixture is stirred and extracted for 2 hours. The obtained extract was concentrated under reduced pressure to obtain gardenia pigment extract 186.6F.

(Solid content 67.7%) This was diluted 10 times with purified water and used as a sample solution. TSB medium (tryptone 15f1 soy peptone 5t, Na (4159), distilled water 1000 ml.

PH7,3), GKMB medium (potato extract - potato 2)
Made from 002-1000-, starch 102, Beefex 6°02, glycose 2.02, pegton 5. Of
, K, HPo, 0.8? , Fe80. -7H,
00,12, glycerol 60.0ml, pH7,0
), YMB medium (Bacto yeast extract 4t,) (Bactomalt extract 102, Bunt textulose 4y, distilled water 1ooo-1PH7,3), add 8 ml each,
06. After autoclaving for 20 minutes, each strain was inoculated with one platinum dose and cultured by shaking at 27°. 24
After 0.5 hours, the gardenia sample solution was sterilized by filtration.
was added to this, and after further shaking culture for 48 hours, the color tone was visually observed. As a result, a significant change in color tone was observed in the strains shown in Table 1.

Example 2 3 tons of the same TSB medium as in Example 1 is prepared, and 49.69 g of the gardenia pigment extract prepared in Example 1 is added thereto. 120 @, after 20 minutes high pressure steam sterilization, 27
300° of Stregutomyces canescens (5P5001) culture solution that had been cultured for 48 hours was added thereto. This was cultured with aeration at 30° and 2500 rpm, and a portion was removed as a sample solution at each hour.
After 0 minutes of high-pressure steam sterilization, the mixture was filtered to obtain a clear liquid.

Table 2 shows the absorbance of these clear liquids in extremely thick absorption near 440 nm and 59 Onm and the color tone as determined by visual observation.

Table 2 After culturing for 120 hours, autoclave sterilized at 120° for 20 minutes.
It was filtered and concentrated under reduced pressure to obtain a blue gardenia pigment conversion product as an extract.

Example 3 Pegton 452, Soy Peptone 152, Nac115f
41.3F of the gardenia pigment extract prepared in Example 1 was added to a medium prepared with 3 tons of distilled water, and 120', 20
After high-pressure sterilization for 1 minute, Strebtomyces miciganensis (from p. 53) was cultured for 27° and 48 hours.
A15) Add 200- of the culture solution. This is 30°
, 2500 ram with agitation and aeration, and uniform treatment was carried out at each time with the procedure f1''2 to obtain the results shown in Table 3.

20. After 60 and 120 hours, a portion of the sample solution was removed, each sample was sterilized and filtered in the same manner as in Example 2 to obtain a clear solution, which was concentrated under reduced pressure to obtain yellow-green, green, and blue gardenia pigments. I was able to get a convertible.

Claims (1)

[Claims] A microorganism belonging to the genus Streptomyces that has the ability to produce a gardenia pigment conversion product different in color tone from the gardenia pigment extract is inoculated and cultured in a medium supplemented with a gardenia pigment extract, and a yellow-green, green or blue gardenia is produced. A method of producing a dye conversion product.