JPS5919858A - Automatic processing device for analysis - Google Patents

Abstract

PURPOSE:To perform automatically analysis and to perform quickly operation with less manpower by making a turntable which supports freely oscillatably a processing vessel freely turnable and stoppable, making common use of the turntable as a centrifugal separator, and performing the respective operations for reaction processing according to the preset program. CONSTITUTION:The entire drawn blood sample is stored in a processing vessel 9, and is installed in a support part 5 for the processing vessel. After the vessel is allowed to stand for a specified time, a turntable 1 is rotated so that sera and blood-clot are separated by centrifugal force. Absorbancy is then measured with light projectors and receivers 16, 17 by which the states of the sera such as the degree of clouding in the supernatant serum part, the degree of hemolysis or the like are inspected. A suction and discharge nozzle 1, and pumps 13, 14 are operated to feed the blood together with a diluent into another processing vessel. A reagent feed nozzle 18 and a pump 19 are operated to feed a reagent by each specified amt. and the mixture is handled with a mixer 26, whereafter the entire part of the processing vessel is contained in a thermostat 27 where the reaction is effected under specified temp. conditions. The reacting liquid is sucked from the processing vessel and is passed through a detector 24, by which the values of absorbancy, etc. are measured.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention mainly relates to an automatic analysis processing device for performing blood biochemical tests.

Generally, when performing biochemical tests on blood, etc., various processes such as centrifugation of the sample solvent, fractionation of the supernatant or precipitated portion, mixing of reagents to the fractionated portion, and measurement of absorbance and other matters are performed in sequence. For example, when analyzing blood prior to a surgical operation, the whole surface of the sample is allowed to stand still for a certain period of time, then centrifuged to separate the blood from the surface, and only the supernatant is stored in a separate processing container. The solution is dispensed in portions, diluted by adding a diluting solution, and the desired reaction solution is added and set in each processing container, and after repulsion, the absorbance and the like are measured using a measuring device.

For such analysis, automated devices have traditionally been developed for measurements after reagent injection, but the steps before this, such as blood separation, reagent injection, and shaking, etc., have been developed. The harvester used different equipment or equipment to perform the samples separately and sequentially, and then applied them to an automatic measuring machine, which resulted in mix-ups of samples during work or errors during transfer. There is a risk of dropping and damaging heavy samples, and furthermore, there is a risk of infectious disease accidents occurring due to scattering of samples during work.Moreover, if there are many work steps and many workers are required, it will take 1 q. However, even in urgent cases, there are problems in that analysis requires a long time, especially at night when there are few personnel.

In view of the above-mentioned drawbacks of the conventional technology, the present invention has been developed to automatically collect a sample, place it in a container, set it in a predetermined position, etc., without any manual intervention by the operator, and perform various pre-measurement procedures. The purpose is to provide an apparatus capable of processing and measuring, and its gist is that it is equipped with a turntable that is equipped with a ladle (high-speed rotation drive) and can be stopped at a desired rotation angle, and that the periphery of the turntable is The turntable is equipped with a large number of processing container support parts that swingably support processing containers at regular intervals, and is arranged above the stop position of any one of the plurality of processing containers of the turntable to suck and drain the liquid in the processing container and to release the reagent. Suction/discharge nozzles for performing various processes such as injection and suction of liquid after reaction processing are provided so that they can be raised and lowered by lifting means, and R/lowering means are provided above and below the stopping position of one or more processing containers. Equipped with a processing container support part freely,
Rotating and stopping the turntable, raising and lowering various nozzles,
The invention resides in an automatic analysis processing device which automatically causes the suction/discharge ffj iff 13J to operate according to a preset program.

Next, an example of implementation of the present invention will be described with reference to the drawings.

In the figure, '1' is a turntable. This turntable 1 is supported to rotate at high speed by a motor 3 around a shaft 2, and a disc 4 for angle detection is fixed to the shaft 2, and a through hole in the disc 4 is connected to a sensor 4a. Detects °C,
The drive of the motor 3 is controlled to ensure that it rotates and stops at the required angle.

Processing container supports 5.5 are provided at regular intervals on the periphery of the turntable 1. The processing container support section 5 is attached to the turntable 1 as shown in FIGS. 2 and 3.
A container support ring 8 is rotatably supported within the recess 6 with a pair of bins 7.7 facing in the rotational direction of the turntable 1. The tip of the bottle 7.7 projects from the inner peripheral surface of the container support ring 8. A processing container 9 is supported within the container support ring 8. A bayonet groove 10 is formed on the outer peripheral surface of the upper end of the processing container 9, and the bottle 7 is inserted into this groove 10 and rotated. The bayonet groove 10 is
A vertical portion 1-Oa is formed at the innermost part of the mold, allowing the bottle 7 and the processing container 9 to move up and down relative to each other.

On the other hand, above the turntable 1, a suction/discharge nozzle 11 for sample transfer is supported so as to be movable up and down by an elevating device 12, corresponding to above the stop position of any position of the processing container support. This suction/discharge nozzle 11 is connected in series with a suction pump 13 for fractionation and a diluent injection pump 14 via a flow path, and its tip is communicated with a diluent tank 15 .

Adjacent to the suction/discharge nozzle 11, an absorbance detection device for inspecting the condition of the specimen is provided. This device consists of a light projector 16 and a light receiver 17 opposed to the light projector 16.
The processing container 9 is positioned between 16 and 17 and moved relative to each other,
The absorbance is measured sequentially from the top to the bottom and recorded on a separate recording device.

Further, a plurality of reagent injection nozzles 18 are provided above other stop positions. Each reagent injection nozzle 18
is fixed to a support substrate 20 that can be moved up and down with a four-lowering device 19. Each of the reagent injection nozzles 18 is connected to a pump 21 via a flow path, and the tip thereof communicates with a reagent tank 22. 1 In addition, the support substrate 20 is provided with suction of the liquid after the reaction of the reagent,
A reaction liquid suction nozzle 25 is provided to feed the reaction liquid to the detector 24. A mixer 26 and a constant temperature bath 27 are provided below the turntable 1, and these are supported by a substrate 28. This board 28 is raised and lowered by two lifting devices provided below.

This mixer 26 is equipped with a processing container 9 supported on a turntable 1, and mixes and stirs a sample and a reagent.As shown in FIG. It consists of a crank 31 attached to a shaft and a single-spin mover 32 rotatably attached to the crank bin position of the crank 31. The motor 30 is driven in a pressed state, and this presser 33 is attached via an elastic member 34 made of rubber or the like.

This apparatus performs processing by sequentially operating each member arranged in the J: section, and the operation is performed using an automatic control BM II incorporating a microcomputer. A blood biochemical test will be explained as an example of its effect.

Prior to processing, the entire surface sampled is placed in a processing container 9 and placed on a predetermined processing container support portion 5 of the turntable 1. Other processing containers 9, 9, . . ., as many as necessary for analysis, are installed in the other processing container support portions 5.5,
After installation in this way, the automatic controller is activated and the operation is performed automatically according to the preset program.

It will remain stationary for a certain period of time after it is placed. This 1i11 position is intended to allow the serum to be separated by 1m.

After this standing still, the motor 3 of the turntable 1 is rotated at high speed. This rotation centrifuges the entire surface of the serum and blood ω1, and when the turntable 1 is rotated, the processing container 9 is swung out in the radial direction of rotation with the bin 7.7 as the center, and a centrifugal force is applied to the entire surface inside. is applied, which separates serum and blood clots.

After performing centrifugation 9-1 in this manner, the condition of the serum such as the cloudiness of the serum portion of the supernatant and the degree of hemolysis is examined. This inspection is carried out as shown in FIG.
17 with the processing container 9 held between them and measuring the absorbance. At the same time, the boundary between the serum portion a and the blood clot portion A is detected, and the nozzle lowering height, which will be described later, is controlled. I try to do it.

After testing the condition of the serum in this way and recording the results, the serum portion of the supernatant is dispensed from inside the processing container 9. For this dispensing, the suction/discharge nozzle 11 is lowered, a certain amount of serum is sucked by the pump 13, and then raised.The turntable 1 is rotated to position another processing container for reaction treatment under the suction/discharge nozzle 11, and then the serum is raised again. The robot descends and activates the pump 14 to inject serum along with a certain amount of diluent.

Similarly, the suction/discharge nozzle 12 is moved up and down, the turntable 1 is rotated, and the pumps 13 and 14 are operated repeatedly to effect dispensing into a desired number of processing vessels.

After dispensing in this manner, reagents are separately injected into each processing container. In order to inject this reagent, the turntable 1 is rotated so that the processing container is located at a position corresponding to each reagent injection nozzle 18, and then the reagent injection nozzle 18 is lowered and the pump 19 is activated to inject a fixed amount of the reagent. I slack off.

Next, the processing containers after injecting the reagents are sequentially applied to the mixer 26. That is, after the reagents are injected, the turntable 1 is rotated to position the processing containers above the mixer 26, and then the mixer 26 is raised to process the gravitational members 32. The presser 33 is brought into contact with the lower end of the container, the suppressor 33 is lowered, and the upper end of the processing container is pressed with one hand, and then the motor 30 is rotated to set the processing container. In the same manner, PM sake work is performed for each processing container.

After performing the installation work for each processing container in this way, the turntable 1 is rotated to make each processing container correspond to the thermostatic chamber 27, and each thermostatic chamber 27 is raised to place the entire processing chamber inside. and react under constant temperature conditions.

This state is maintained for the time required for the reaction, and then the reaction liquid suction nozzle 25 is lowered to suck the reaction liquid from the processing container, and the reaction liquid is passed through the detector 24 to measure values such as absorbance.

This measurement is performed separately for each reaction solution in each processing container and the data is recorded.

In the above embodiment, a constant temperature bath is provided under the turntable, but it is also possible to place the entire device in a constant temperature bath. In addition, the processing container itself is housed under the turntable, and the detector that measures absorbance etc. through the external processing container can be moved up and down, and the turn table can be rotated and stopped. Alternatively, the detection may be performed sequentially.

The automatic analysis processing device of the present invention is constructed as described above, and includes a turntable that supports a processing container in a swingable manner, and a turntable that can freely rotate and stop while indexing the supporting portion of the processing container. At the same time, the turntable was designed to rotate at a desired angle and to be able to rotate at high speed so that it could also be used as a centrifugal separation device. By doing so, each of these operations is performed according to a preset program.By doing so, complete automation of the analysis process has been achieved, and the drainer can automatically analyze the sample by simply The desired objectives were achieved, such as the production was carried out quickly with a small number of workers, there was less chance of losing specimens due to carelessness on the part of the workers, and there were fewer accidents caused by infectious diseases. be.

[Brief explanation of drawings]

The drawings show an embodiment of the present invention, and FIG. 1 is a partially omitted longitudinal sectional view, FIG. 2 is a plan view of the turntable, FIG. 3 is an enlarged sectional view of the Tahari container support, and FIG. A partial side view of the processing container, FIG. 5 is an absorbance test P' for inspecting the condition of the specimen.
FIG. 6 is a longitudinal sectional view showing the mixer in use. DESCRIPTION OF SYMBOLS 1... Turntable, 2... Motor, 5... Processing container support part, 7... Bin, 8... Container support ring , 9... Processing container, 11... Suction/exhaust nozzle, 18...
・Reagent injection nozzle, 24...Detector, 25...
... Reaction liquid suction nozzle, 26 ... Mixer, 33 ... Presser.

Claims (1)

[Claims] It is equipped with a turntable that is equipped with a high-speed rotation drive ISM trli and can be stopped at a desired rotation angle, and a large number of processing container supports that swingably support processing containers are provided at regular intervals on the periphery of the turntable. , suction and discharge nozzles for performing various processes such as suction and discharge of liquid in the processing vessel, injection of reagents, and suction of liquid after reaction processing are respectively installed above the stop position of one of the plurality of processing vessels of the turntable. Each of the turntables is provided so as to be freely lentable and lowerable by an elevating means, and a processing container support portion is provided above and below the other one or a plurality of processing container stop positions so as to be able to be freely raised and lowered by the elevating means, and rotation of the turntable,
An automatic analysis processing device that automatically stops, lifts and lowers various nozzles, and operates suction/exhaust points according to a preset program.