JPS59196086A - Preparation of marine chlorella - Google Patents
Preparation of marine chlorellaInfo
- Publication number
- JPS59196086A JPS59196086A JP6920583A JP6920583A JPS59196086A JP S59196086 A JPS59196086 A JP S59196086A JP 6920583 A JP6920583 A JP 6920583A JP 6920583 A JP6920583 A JP 6920583A JP S59196086 A JPS59196086 A JP S59196086A
- Authority
- JP
- Japan
- Prior art keywords
- content
- marine chlorella
- sodium chloride
- chlorella
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Description
【発明の詳細な説明】
本発明はエイコサペンタエン酸（以下、ＩＦ人と略す）
含量が、総脂肪酸の３分の１以上となるような海産クロ
レラの培養法に関する。[Detailed Description of the Invention] The present invention relates to eicosapentaenoic acid (hereinafter abbreviated as IF acid)
The present invention relates to a method for culturing marine chlorella such that the content is one-third or more of the total fatty acids.
従来、ＥＰムは魚油から精製されているが、魚油はＥＰ
Ａ以外の高度不飽和脂肪酸でＥＰムに類似の構造を持つ
脂肪酸を多く含むため、分離精製が大変困難である。Conventionally, EP-mu is refined from fish oil, but fish oil is
It is a highly unsaturated fatty acid other than A and contains many fatty acids with a structure similar to that of EP, so it is very difficult to separate and purify it.
一方、海産クロレラは古くから養魚飼料用に利用されて
きたが、近年その中にＥＰ人が見出され注目されるよう
になった。海産クロレラの培養法は、従来天然海水にα
０５チ以下の窒素源、リン酸源等を加えて養魚、飼料用
を目的とした培養が行われたが、ＩＣＰム含量は最大で
クロレラ中の総脂肪酸の３０％程度のものしか得られず
、これが限界であった。また、屋外池での培養であった
ため温度コントロールが難しく、ＥＰム含量は１５〜３
０俤の間で大きく変動していた。On the other hand, marine chlorella has been used for fish feed for a long time, but in recent years EP has been discovered in it and has attracted attention. Traditionally, the cultivation method for marine chlorella is based on natural seawater.
Cultivation was carried out for the purpose of fish farming and feed by adding less than 0.05% nitrogen source, phosphoric acid source, etc., but the maximum ICP content was only about 30% of the total fatty acids in chlorella. , this was the limit. In addition, since the culture was carried out in an outdoor pond, it was difficult to control the temperature, and the EP content was 15 to 3.
It fluctuated widely between 0 and 0.
本発明者らは鋭意研究を重ねた結果、海産クロレラの培
養において培地中の総塩化ナトリウム含量および培養温
度により総脂肪中のＥＰム含量が大きく変動することを
見い出した。As a result of extensive research, the present inventors found that in culturing marine chlorella, the EP content in total fat varies greatly depending on the total sodium chloride content in the medium and the culture temperature.
本発明はかかる知見に基づいて完成されたもので、培地
中の総塩化す） ＩＪウム含量が０．１乃至２゜０％で
ある人工海水培地を用い、培養温度を１５乃至２５℃と
することを特徴とする海産クロレラの培養法である。The present invention was completed based on this knowledge, and involves using an artificial seawater culture medium with a total IJ content of 0.1 to 2.0% in the culture medium, and culturing at a temperature of 15 to 25°C. This is a method for culturing marine chlorella that is characterized by the following.
ここで人工海水培地とは窒素源、リン酸源、マグネシウ
ム、カリウム、。カルシウム及び他の微量金属元素を含
む公知の培地をいう。Here, the artificial seawater culture medium is a nitrogen source, a phosphoric acid source, magnesium, potassium, etc. A known medium containing calcium and other trace metal elements.
培地中の塩化ナトリウム含量は２．０俤を超えると菌体
の生育量および８２人含量がともに低下する傾向にある
。従って、天然海水（塩化ナトリウム含量約＆５％）で
は塩化ナトリウム濃度が高過ぎて菌体生育量、ＥＰム含
量とも低くなっている（実施例１のＩ）。また、塩化ナ
トリウム含量が０．１％未満であると同様に菌体生育量
およびＥＰＡ含量が低下′する（実施例１のムおよびＢ
）。しかしながら、人工海水培地に塩化ナトリウムその
ものを添加する代わりに相当量の天然海水を用いること
も可能である。また、天然海水を用いた方が微量金属元
素をあらためて添加する必要が無いので好都合でもある
。When the sodium chloride content in the medium exceeds 2.0 t, both the bacterial growth amount and the bacterial cell content tend to decrease. Therefore, in natural seawater (sodium chloride content: about &5%), the sodium chloride concentration is too high, and both the bacterial growth amount and the EP content are low (I in Example 1). Furthermore, if the sodium chloride content is less than 0.1%, the bacterial cell growth amount and EPA content will similarly decrease (M and B in Example 1).
). However, instead of adding sodium chloride itself to the artificial seawater medium, it is also possible to use a considerable amount of natural seawater. Furthermore, it is more convenient to use natural seawater because there is no need to add trace metal elements again.
培養温度が１５℃未満では生育速度は極めて遅く、また
２５℃を超えるとＨＰム含量の低下が見られ、５０℃以
上では量化現象（部分的死滅）が起きてくる。If the culture temperature is less than 15°C, the growth rate is extremely slow, if it exceeds 25°C, a decrease in HPum content is observed, and if it is 50°C or higher, a quantification phenomenon (partial death) occurs.
本発明の培養法によればＩＦム含量を、これまでの限界
を超えてクロレラ中の総崩肪酸の３分の１以上とするこ
とができ、しかも安定的な生産が可能である。また、従
来法に比べ、菌体の増殖速度が速く、菌体の生育量も大
きい。According to the culture method of the present invention, the IFM content can be increased to one-third or more of the total fatty acids in chlorella, exceeding the previous limit, and stable production is possible. Furthermore, compared to conventional methods, the growth rate of bacterial cells is faster and the amount of bacterial growth is larger.
以下に実施例を示す。Examples are shown below.
実施例１
基本人工海水培地（以下Ｂ五ｓ’ｗという。組成は表−
１に示す）に種々の量の塩化ナトリウムを添加したもの
を用いて海産クロレラの培養を行なった（表−２に各培
地組成を示す）。同一濃度の海産クロレラを各培地（殺
菌済）に接種した後、温度２０℃同一光及び同一照度条
件（約１万ルクス）で、６日間培養を行なった。培養期
間終了後、遠心分離で菌体を集め、洗浄後、ヘキサン：
イソプロパツール；５：２で脂質抽出を行なった。Example 1 Basic artificial seawater culture medium (hereinafter referred to as B5s'w. The composition is shown in Table-
Marine chlorella was cultured using chlorella (shown in Table 2) to which various amounts of sodium chloride were added (Table 2 shows the composition of each medium). After inoculating each medium (sterilized) with marine chlorella at the same concentration, culture was performed for 6 days at a temperature of 20° C. and under the same light and illuminance conditions (approximately 10,000 lux). After the culture period is over, the bacterial cells are collected by centrifugation, washed with hexane:
Lipid extraction was performed with isopropanol; 5:2.
※微量元素溶液
最終菌体生育量（ｇ ｗｅｔ ｏｅｌ、１／ ｌ ）お
よびＫＰム含有量（％ 総崩肪酸中）を表−３に示す。*Table 3 shows the final bacterial cell growth amount (g wet oel, 1/l) of the trace element solution and the KPM content (% in total fatty acids).
表−３培地と最終菌体生育量、ｘｐム含量の関係実施例
２
ＢＪＬ８Ｗに対し０．５％の塩化ナトリウムを添加した
培地を用い、培養温度を１０〜５０Ｃまで変化させた場
合の菌体生育量およびＨＰム含量の変動を調べた。その
他の照度条件、脂肪抽出方法は実施例１と同様である。Table 3 Relationship between culture medium, final bacterial cell growth amount, and xpm content Example 2 Bacterial cells when the culture temperature was varied from 10 to 50 C using a medium containing 0.5% sodium chloride for BJL8W Changes in growth amount and HPum content were investigated. Other illuminance conditions and fat extraction method are the same as in Example 1.
Claims (1)
トリウム含量が０．１乃至２．０％である人工海水培地
を用い、培養温度を１５乃至２５℃とする仁とを特徴と
する海産クロレラの培養法。(1) In culturing marine chlorella, an artificial seawater medium with a total sodium chloride content of 0.1 to 2.0% is used, and the culture temperature is 15 to 25°C. culture method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6920583A JPS59196086A (en) | 1983-04-21 | 1983-04-21 | Preparation of marine chlorella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6920583A JPS59196086A (en) | 1983-04-21 | 1983-04-21 | Preparation of marine chlorella |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59196086A true JPS59196086A (en) | 1984-11-07 |
| JPS6110117B2 JPS6110117B2 (en) | 1986-03-28 |
Family
ID=13395984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6920583A Granted JPS59196086A (en) | 1983-04-21 | 1983-04-21 | Preparation of marine chlorella |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59196086A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6087798A (en) * | 1983-10-21 | 1985-05-17 | Meiji Milk Prod Co Ltd | Production of eicosapentaenoic acid by algae |
| JP2014223024A (en) * | 2013-05-15 | 2014-12-04 | 日本電信電話株式会社 | Culture method and culture apparatus of microalgae |
-
1983
- 1983-04-21 JP JP6920583A patent/JPS59196086A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6087798A (en) * | 1983-10-21 | 1985-05-17 | Meiji Milk Prod Co Ltd | Production of eicosapentaenoic acid by algae |
| JPH0438397B2 (en) * | 1983-10-21 | 1992-06-24 | ||
| JP2014223024A (en) * | 2013-05-15 | 2014-12-04 | 日本電信電話株式会社 | Culture method and culture apparatus of microalgae |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6110117B2 (en) | 1986-03-28 |
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