JPS59187381A - Solid vascular specimen for internal organ - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides a three-dimensional vascular structure specimen of an organ, particularly an organ made of silicone rubber that is easy to work with when preparing the specimen, the prepared specimen is highly accurate, and the internal structure can be observed. This study concerns three-dimensional vascular structure specimens.

In the past, mold specimens of organs were mainly used for morphological purposes, but in recent years, they have been increasingly used for clinical anatomy and clinical pathological anatomical searches. In addition to cerebral blood vessels, heart (coronary) auxiliary vessels, limb vessels, etc., lungs,
Research is also progressing on injection agents and injection methods for making mold specimens of Ill vessels such as liver crotch, kidney, and tile 11 (:1).Various materials have been studied for this injection, and recently Synthetic resins began to be used,
Currently, methods using celluloid resin, polyester resin, acrylic resin, etc. have been developed, and many documents have been published. In addition to the disadvantage that the precision of specimens is poor due to the high shrinkage rate during curing, this type of material has a short pot life, making it difficult to select a material with an appropriate pot life, and This method has disadvantages such as not being able to obtain an appropriate viscosity for each product, resulting in poor workability and problems with colorability.Furthermore, specimens made from this have poor 0JJe properties and are easily damaged. They are usually stored in transparent containers and must be handled with great care; therefore, they rarely have a serious practical drawback in that it is impossible to touch them with your hands to closely examine their internal structure.

The present invention relates to a three-dimensional vascular structure specimen of an organ that solves these disadvantages, and is formed by using a silicone rubber composition as a three-dimensional specimen material (injection agent, replication material). This is a characteristic feature.

To explain this, the present inventors have conducted various studies to improve the three-dimensional specimens of organs that have the various drawbacks mentioned above, and have found that if a silicone rubber composition is used as the material for the three-dimensional specimens, the shrinkage rate upon curing will be lower. In addition to being able to obtain highly accurate specimens because of its small size, this silicone rubber composition is easy to work with as it allows you to freely select pot life and viscosity, and it also allows you to freely color it to your favorite color. What's more, the three-dimensional specimens made from these specimens are flammable, strong, and hard to damage, so they can be touched freely.

Therefore, the present invention was completed after confirming that the internal structure can be observed freely.

The silicone rubber composition used to create the three-dimensional vascular structure specimen of an organ according to the present invention is one that can be cured under relatively mild conditions since the object of the three-dimensional specimen is an organ. It is therefore particularly preferred that this be described below.

That is, this silicone rubber composition has A) an average compositional formula of R'aSiO,a (where R is a monovalent organic group, a is 1.8 to 21), and a silicone rubber at both ends of the molecular chain. Chain diorganopolysiloxane with aliphatic unsaturated groups
100M portion B) 0.5 to 5 times the amount of aliphatic unsaturated groups in the diorganopolysiloxane of A) above, which has a small layer of hydrogen atoms bonded to silicon atoms in the molecule. One: Si
Organohydrodiene polysiloxane containing H groups C) Silica-based filler 0-50. iJj parts catalytic amounts of addition reaction catalysts, or a) an average compositional formula of R2bSio4-b (where R2 is 1);
A chain diorganopolysiloxane in which both ends of the molecular chain are blocked with hydroxyl groups, where b is a valent organic group and b is 1.8 to 2.1). Fewer hydrolyzable atoms or groups? ) ~ Organosilicon compound having 0.1 to 20 parts by weight C) Silica-based filler O to 50 parts by weight d Condensation reaction catalyst such as organic acid metal salt or amine compound
A composition consisting of 0 to 5 parts by weight is used.

To explain each component constituting the above-mentioned composition in more detail, the diorganopolysiloxane as the component A) is, for example, an organopolysiloxane represented by the formula monovalent hydrocarbon radical (l is a positive integer). is exemplified, and this B
) component is an organic compound having two or more, preferably three or more -ESi-H groups in its molecule, which is represented by the formula (where KR is the same as above, m and n are positive integers). An example of this is organohydrodienepolyloxane, which is added in an amount of 0.5 to
This is the amount that provides 5 times the amount of =siH groups, and is usually 0.1 to 10 parts by weight per 100 parts by weight of component A).

In addition, this component C) is mainly added to reinforce the silicone rubber, and although it is not necessarily an essential component, it is
=41. Maru, just one.

Any of two-method silica, wet-process silica called precipitated silica, diatomaceous earth, quartz powder, and fused silica powder may be used, and these also have trimethylsilylated hydroxyl groups bonded to silicon atoms on their surfaces. The surface of the material may be treated to make it water repellent with dimethylpolysiloxane having a low degree of polymerization. D) Pt, pd for addition-reactive catalysts as components.

Examples include Rd6 or these compounds. Specifically, these include chloroplatinic acid, alcohol-modified chloroplatinic acid, a complex of chloroplatinic acid and an olefin, platinum black, or solid platinum on a carrier such as alumina or silica. Examples include a rhodium complex and a rhodium complex. In addition, when using the composition consisting of these people) to D), from the viewpoint of workability (pot life),
No. 47, Japanese Patent Publication No. 54-3774, U.S. Patent No. 3.
It is preferred to use addition reaction control agents such as those described in US Pat. No. 445,420.

Further, as the component a) mentioned above, diorganopolysiloxanes represented by the formula % (where R is the same as above and p is a positive integer) are exemplified, and the organosilicon compounds as the component b) are exemplified as above. It is essential to use a crosslinking agent for the diorganopolysiloxane as component a). Examples of this hydrolyzable group include an alkoxy group, an acyloxy group, a ketoxime group, an amino group, an aminoxy group,
These include amide groups, alkenyloxy groups, etc., and component b) may be either a monomer or a polymer; generally, this should be a low molecular weight polymer;
good.

In addition, the silica filler as component C) is exactly the same as component 90) described above, so the explanation will be omitted, but component d) is a component that reacts with the curing reaction of component a) and component b). It is used to promote dibutyltin dilaurate.

Dibutyltin dioketoate, tinoff), :C-).

Examples include metal salts of organic acids such as iron octoate, and amine compounds such as dibutylamine, triethanolamine, and tetramethylguanidine.

In addition, this silicone rubber composition consisting of the above-mentioned A) to D) and components a) to d) is further mixed with R3Si Oo.
, 5 units and Sigh units, or Rs S i 0
o-s unit, R25iO unit and 5iOz unit, this R3Si 0o-s unit or R3Si Oo
, s units and the molar ratio of R25iO units to SiO2 units is 0.5 to 1.5, and an organopolysiloxane containing an aliphatic unsaturated group or a -1siOH group bonded to a silicon atom in the molecule. Even if it is added, it is further added with a low degree of polymerization to improve the dispersibility of the above-mentioned component C) and the silica filler as the component C), and hydration to remove iOH groups or halogens at the terminals. Diorganopolysiloxane with degradable groups.

Todiphenylsilanediol, dimenaldieken disilane, etc. may be added, and the adhesion of inorganic materials other than silica, organic fillers, 2 colorants, carbon functional silanes, etc. within the range that does not impair its physical properties. It may also contain additives such as imparting agents, fillers, and other additives that are usually blended into silicone rubber compositions. method, which can be obtained by e.g. rCHEMIST)ζY ANDTECf(
NOLOGY OF 5ILICONES J (Wa
by iterNOLL, Academic Press, 1968
(published in 2013).

There are no particular limitations on the way the noricorn rubber composition can be used in the present invention, but if the three-dimensional specimen needs to be formed in detail, such as the peripheral branches of the capillaries of the organ, or if this organ is not easily available. Because it is a product, if you are trying to make a mother mold first for mass production and then try to reproduce it, it is best to use a mold with relatively low viscosity, and in other cases, Low viscosity, e.g. 200~
It is preferable to use one with 900 voices.

Next, we will describe the method for preparing a three-dimensional vascular structure specimen of a liA device according to the present invention. This involves injecting the silicone rubber composition described above into the vessel of the organ for which a three-dimensional specimen is to be created, and then injecting the silicone rubber composition into the vessel of the organ for which the three-dimensional specimen is to be created. If you want to mass-produce, you can make a mother mold based on the original specimen and reproduce it, but when creating this prototype specimen, make sure that the organs do not deform. It is important to keep the deformation in mind, especially in cases where the three-dimensional interrelationship of blood vessels is important, such as the liver and lungs. To prevent this, it is necessary to take measures to prevent deformation depending on the type of organ. Conventionally, this has been done by, for example, supporting the organ in warm water with gauze, or making gauze in the approximate shape of the organ in advance and placing the organ in the gauze. A method of keeping the inner body still has been used, but this method uses computerized tomography (COM).
pulled monography = C, T, )
, Ultrasound diagnostic percutaneous transhepatic portal venography (P, T, P,
), it is best to know the exact three-dimensional relationship of each vessel in advance, and then create and use a tool to correct and maintain this deformation.

Next, examples of the present invention will be given, in which Me represents a methyl group, Vl represents a vinyl group, Bu represents a butyl group, and the viscosity is a value at 25°C.

Example 1 (Preparation of liver vascular three-dimensional structure specimen) 67.5 parts by weight of dimethylpolysiloxane, Me3s, having a viscosity of 5.0 OocS and having both molecular chain ends capped with vinyl groups, whose average molecular formula is shown by the following formula: i 00.5 units 42.5 molti, M
8□Vi St 0n-s units 7.5 mol% and 5i
6.5 parts by weight of methylvinylpolysiloxane consisting of 50 moles of n2 units, ((CH2=CH)CH38 people 40.
1 part by weight, 7 parts by weight of fumed silica with a specific surface of 8150 M/g surface-treated with hexamethyldisilazane, and an n-butanol solution of chloroplatinic acid (platinum concentration 1 weight 1).
After uniformly mixing 0.1 parts by weight, 4 parts by weight of methylhydrodiene polysiloxane represented by the following formula was added and mixed uniformly to form a silicone rubber composition (A
)made.

Next, this composition (A) ioo g was mixed with black red iron.

1 g of Cobalt Blue, GC Let, and Harmanent Yellow were added and mixed uniformly with a triple roll to form compositions of four colors, which were defoamed under vacuum.

Next, we will create a three-dimensional specimen of the portal vein of the liver removed at autopsy.

After intubating the hepatic artery, bile duct, and vena cava with a cannula to remove as much air as possible from each vessel, physiological saline is perfused, and any leakage of physiological saline during perfusion is closed with sutures. The silicone rubber composition was injected into the portal vein cannula in blue, the hepatic artery cannula in red, the bile duct cannula in yellow, and the hepatic vein cannula in black. In this case, the injection amount is 1.10
0 to 1.300 g of liver, 65 to 80 g to portal vein, 8 to 12 g to hepatic artery 1c, 8 to 12.9 to bile duct, 6 to hepatic vein.
0 to 70 g was injected, and the injection was carried out slowly while appropriately changing the direction of the silicone rubber composition in order to eliminate unevenness due to gravity of the silicone rubber composition.

After this injection is completed, use a deformation prevention device similar to the shape of the diaphragm to restore it to the same shape as it was in the body as much as possible, then place it in water together with the deformation prevention device, and finish it with gauze. The shape of the liver was adjusted and left for about 24 hours to cure the silicone rubber composition.

After curing of this silicone rubber composition, the liver parenchyma was removed, thoroughly washed with water, and dried at room temperature.A specimen of the vascular 3D structure of the liver was obtained, which was extremely accurate and possible. It was highly practical, as it was not susceptible to burning and would not deform or damage even if touched directly, making it possible to observe the internal structure.

Example 2 87 parts by weight of dimethylpolysiloxane whose average molecular formula is represented by the following formula, 2 parts by weight of dimethylpolysiloxane whose average molecular formula is represented by the following formula, and 33 parts by weight of dimethylpolysiloxane whose average molecular formula is represented by the following formula: Part by weight, specific surface area treated with hexamethyldisiloxane is approximately 18
07/I! In the same manner as in Example 1, 1 g of four types of colorants were added to 100 g of a silicone rubber composition obtained by uniformly mixing 26 parts by weight of fumed silica to prepare silicone rubber compositions colored in four different colors. Then, 4g of (CsH70)4St and Bu
A silicone rubber composition (B) was prepared by adding 21 g of 2sn (OCo C1l H23).

Next, these four kinds of silicone rubber compositions (B) were prepared in the same manner as in Example 1, using the portal vein and hepatic artery of a liver removed at autopsy.

injected into a cannula inserted into the bile duct and hepatic vein,
When we made a three-dimensional specimen of the vascular structure of the liver, it was found to be of extremely high precision, as was the case with Jihyo, and since it is not flammable and does not deform or damage even when touched directly, we were able to observe its internal structure. It was possible.

Patent applicant Shin-Etsu Chemical Co., Ltd. Procedural amendment 1. Table of cases 4 <Patent 8 No. 6 of 1982] 879 No. 2, Name of the invention Three-dimensional vascular structure specimen of an organ 3, Person making the amendment Relationship to the case Patent applicant name (206) Shin-Etsu Chemical Co., Ltd. 4 Agent 6, Amended lunar specification 7, The entire text of the amended description is amended as attached, Description 1, Name of the invention Specimen of three-dimensional vascular structure of an organ 2, Claims 1 Made from silicone rubber Sample 2 of vascular three-dimensional structure of an organ, silicone rubber has A) average compositional formula or Ra8104-□ (where R is a monovalent organic group, a is 18-2), and contains fat at both ends of the molecular chain. 100 parts by weight of linear diorganopolysiloxane containing group unsaturated groups (B) in the molecule (= having at least two hydrogen groups bonded to silicon atoms, aliphatic unsaturated groups in diorganopolysiloxane of A) above Organohydrodienepolysiloxane in an amount containing SiH groups in an amount of 05 to 5 times the amount of saturated groups 0) Silica-based filler with a specific surface area (BET method) of 5 om'/g or more O to 50 parts by weight D Organ vascular three-dimensional structure specimen 3 according to claim 1, which is obtained from a composition comprising a catalyst for an addition reaction in an amount of a solvent, wherein the silicone rubber has a) an average compositional formula of Rb si OA: , (where l2R is a monovalent organic group, b is 1.8 to 2.1), a linear diorganopolysiloxane in which both ends of the molecular chain are blocked with hydroxyl groups 100 heavy phase part b) Organosilicon compound having at least two hydrolyzable atoms or groups bonded to a silicon atom in the molecule 0.1 to 20 parts by weight Silica type with a surface area (BET method) of 507111'/, 9 or more Filler O~50 parts by weight d Okipart Condensation reaction catalyst such as acid metal salt, amine compound, etc.
Detailed Description of the Invention The present invention relates to a three-dimensional vascular structure specimen of an organ obtained from a composition comprising 0 to 5 parts by weight, In particular, it relates to three-dimensional vascular structure specimens of organs, which are easy to work with when preparing specimens, have high precision, and allow observation of internal structures.

In the past, mold specimens of organs were put to practical use primarily for morphological purposes, but in recent years, they have become more widely used due to the need for anatomical searches for clinical anatomy and clinical pathology. Research is also progressing on injection agents and injection methods for creating mold specimens of organs such as cerebrovascular vessels, heart (coronary) vessels, extremity vessels, as well as lungs, liver, kidneys, and other organs. For this injection agent, divine materials have been considered, and recently synthetic resins have come to be used.Currently, methods using celluloid resin, polyester resin, acrylic resin, etc. have been developed, and there are many methods. Although literature has been published, three-dimensional vascular structure specimens of organs made with these materials have a drawback in that the precision of the specimens is poor due to the large shrinkage rate during hardening.

In addition, this type of material has short pot life, it is difficult to select a material with an appropriate pot life, and it is not possible to adjust the viscosity to the appropriate level depending on the application. It is difficult to use, has problems with coloring, and specimens made from it are less flexible and easily damaged.
Usually stored in transparent containers, they had serious practical drawbacks, such as the need for extreme care when handling them, and the inability to directly touch them and observe their internal structure.

The present invention relates to a three-dimensional vascular structure specimen of an organ that solves these disadvantages, and is characterized in that it is formed using a silicone rubber composition as a three-dimensional specimen material (injection agent, replication material). It is something.

The present inventors have conducted various studies to improve the three-dimensional specimens of organs that have the various drawbacks described above, and have found that this rubber composition has good workability, allowing for easy selection of pot life and viscosity, and that It can be freely colored in the color of
Furthermore, since the three-dimensional specimen created from this is flexible, strong, and difficult to damage, it is possible to touch the specimen with two hands and therefore freely observe its internal structure. After confirming this, the present invention was completed.

Since the object of this three-dimensional specimen is organ III, the silicone rubber composition used to prepare the three-dimensional vascular structure specimen of an organ of the present invention is a relatively mild silicone rubber composition as described below. It is preferable to use a material that can be cured under suitable conditions.

That is, this silicone rubber composition has: A) an aliphatic compound at both ends of the molecular chain, which has an average compositional formula of R'5iO (where R1 is a monovalent organic group, and a is 1.8 to 21); 100 parts by weight of chain diorganoporin loxane having an unsaturated group B) The aliphatic unsaturated group in the diorganopolysiloxane of A) above, which has at least two hydrogen atoms bonded to a silicon atom in the molecule. against 0
, 5 to 5 times the amount of organohydrodiene polysiloxane containing SiH groups 0) The above silica filler with a specific surface area (BET method) of 50 m''/9
A composition consisting of an addition reaction catalyst in an amount of ~50 parts by weight, or a plow-shaped diorganopolymer whose molecular chain ends are blocked with hydroxyl groups, represented by a monovalent organic group, b is 18 to 21) Siloxane 100 parts by weight b) Organosilicon compound having at least two hydrolyzable atoms or groups bonded to silicon atoms in the molecule 0.1 to 20 parts by weight ga top (7)
Silica filler 0 to 50 parts by weight d Okipart Condensation reaction catalyst such as acid metal salt, amine compound, etc.
A composition comprising 0 to 5 parts by weight is mentioned.

The above component A) is exemplified by the organoporin lochitin represented by the formula (where R is the same or different unsubstituted or substituted monovalent hydrocarbon group, l is a positive integer).

Component B) is an organic compound having two or more, preferably three or more, 28i-H groups in its molecule, for example, represented by the formula (where R is the same as above, co, and n are positive integers). Organohydrodiene polysiloxane is exemplified, and the amount added is 0.5 to 0.5 to the vinyl group in component A).
This is the amount to impart 5 times the amount of Mi-81H groups, and is usually 01 to 0 parts by weight per 100 parts by weight of component A).

Component C) is added mainly to reinforce silicone rubber, and although it is not necessarily an essential component, this silica filler has a specific surface area fBE of
T method) is preferably 5 (Jm''/g or more, examples of which include fumed silica and precipitated silica. These silicas have trimethylsilylated hydroxyl groups bonded to silicon atoms on the surface. The surface of the material may be treated to make it water repellent with dimethylpolysiloxane having a low degree of polymerization.

D) Pt, Pd for addition-reactive catalysts as components
, l or these compounds, specifically, chloroplatinic acid, alcohol-modified radical platinum acid, a complex of chloroplatinic acid and olefin, platinum black, or solid platinum supported on a carrier such as alumina or silica. Examples include rhodium complex and rhodium complex.

In addition, when using a composition consisting of these people) to D), from the viewpoint of workability (pot life), the
It is preferable to use addition reaction control agents such as those described in No. 94.7, Japanese Patent Publication No. 54-3774, and US Pat. No. 3,445,420.

Furthermore, the above-mentioned component a) is exemplified by diorganopolysiloxane represented by the formula (where R is the same as above and p is a positive integer).

The organosilicon compound as component b) acts as a crosslinking agent for the diorganopolysiloxane as component a), and this compound contains at least two hydrolyzable atoms or groups in the molecule. It is mandatory to do so. Examples of the hydrolyzable group include an alkoxy group, an acyloxy group, a ketoxime group, an amino group, an aminoxy group, an amide group, and an alkenyloxy group. In addition,
This component b) may be either a monomer or a polymer, but in general (the second component is preferably a low molecular weight polymer).

Since the adhesive filler as component C) is exactly the same as component C) described above, the explanation thereof will be omitted. Component d) is used to accelerate the curing reaction between component a) and component b), and includes dibutyltin dilaurate, dibutyltin dioketoate, tin octoate,
Examples include metal salts of organic acids such as iron octoate, and amine compounds such as dibutylamine, triethanolamine, and tetramethylguanidine.

In addition, this silicone rubber composition consisting of the above-described components A) to D) and components a) to d) is coated with R35in0.
It consists of 5 units and 810□ units, or R35iOo, s units, R25iO units and SiO□ units, and the molar ratio of the R3810o, 5 units or R35iOo, 5 units and R25iO units and 810□ units is 0.5.
~1.5, and an organoporin loxane containing an aliphatic unsaturated group bonded to a silicon atom or an =8i0H group in its molecule is added, as well as the above-mentioned O) ESi is added to the end of the silica filler with a low degree of polymerization to improve the dispersibility of the silica filler as a component.
Diorganopolysiloxane, diphenylsilanediol, dimethylshedoxinlan, etc. having a hydrolyzable group other than OH group or halogen may be added, and quartz powder, fused Silica-based fillers such as quartz powder and diatomaceous earth, inorganic and organic fillers other than silica-based fillers, coloring agents, adhesion agents such as carbon functional silane, extenders, and other materials commonly added to silicone rubber compositions. It may also contain other additives, and these additions are optional.

Each of the components constituting the silicone rubber composition described above can be obtained by a known method, for example.
OGY OF 5IL100NESJ (Walte
r N0LL, published by Academic Press, 1968).

There is no particular limit to the viscosity of the silicone rubber composition used in the present invention, but if it is necessary to form this three-dimensional specimen down to the smallest details such as the terminal branches of the capillaries of an organ, or if this organ is easily available. Since it is not possible,
If you are trying to make a mother mold first for mass production and then try to reproduce it, it is best to use a mold with a relatively low viscosity.In other cases, it is best to use a mold with a relatively high viscosity, such as 200 mm.
-900 voices are preferred.

Next, the method for preparing a three-dimensional vascular structure specimen of an organ according to the present invention will be described. After injecting the above-mentioned silicone rubber composition into the blood vessel of the organ for which a three-dimensional specimen is to be created, this silicone rubber composition is cured, Then this organ, for example, rots,
Methods such as boiling, decomposition by living things, decomposition with alkali or acid, and using digestive enzymes are sufficient.If you want to mass-produce this specimen, you can make a matrix based on the prototype specimen. All you need to do is make a copy, but when creating this prototype specimen, it is important to avoid deforming the organ, especially for organs such as the liver and lungs where the three-dimensional relationship of blood vessels is important. For this reason, it is necessary to take measures to prevent deformation depending on each organ, and conventionally, for example, the organ is supported in warm water with gauze, or gauze that has been shaped into the approximate shape of the organ is used. Methods such as creating a gauze and holding the organ in place within this gauze have been used, but this method requires computer tomography.
ed monography = O, T, ), ultrasonic diagnostic percutaneous transhepatic portal venography (P, T, P,) 7 Knowing the exact three-dimensional relationship of each vessel in advance, this deformation can be It is best to make and use a device to hold the correction.

Next, examples of the present invention will be given. In the examples, Me is a methyl group, (1) is a vinyl group, and Bu is a butyl group, and the viscosity is a value at 25°C.

Example 1 (Preparation of liver vascular three-dimensional structure specimen) Dimethylpolysilochitin 67, whose average molecular formula is shown by the following formula, has a viscosity of 5.uo.c.8, and has both molecular chain ends capped with vinyl groups.
．． 5 parts by weight, 42.5 mol% Me3s10oII units,
M e 2 Vi S i Oo, s unit 7.5 mol%
and 6.5 parts by weight of methylvinylpolysiloxane consisting of 50 mol% of 51o2 units, ((OH2=OH) 0
H3Si0) 40.1 parts by weight, 7 parts by weight of fumed silica with a specific surface area of 150 m"/g surface-treated with hexamethyldisilazane, quartz powder (Crystallite VXS)
(manufactured by Ryumori Co., Ltd.) 100 parts by weight and n of chloroplatinic acid
- After uniformly mixing 0.1 parts by weight of a butanol solution (platinum concentration 1% by weight), 4 parts by weight of methylhydrodiene polysiloxane represented by the following formula was added and mixed uniformly, and the silicone rubber composition was placed in a container. made.

Then, this composition (A) 100. ! Add Black Red Garla, Cobalt Flu, GO Rele, and Permanent Yellow to it' and mix uniformly with three rolls.
A seed color composition was prepared and vacuum defoamed.

Next, a cannula is inserted into the portal vein, hepatic artery, bile duct, and vena cava of the human autopsy removed liver to create a three-dimensional specimen, and after removing as much air as possible from each vessel, physiological saline is perfused. The leaking part of the physiological saline was sutured and closed.
Then, the silicone rubber composition was injected into the portal vein cannula in blue, the hepatic artery cannula in red, the bile duct cannula ζ in yellow, and the hepatic vein cannula in black. In this case, the injection amount is 1.1oo~
t, 3o (for l liver, 65-80g in the portal vein, 8-12g in the hepatic artery, 8-12g in the bile duct, 60-70g in the hepatic vein
In order to prevent unevenness of the silicone rubber composition due to gravity, the injection was carried out slowly while changing the direction of the silicone rubber composition as appropriate.

After this injection is completed, use a deformation prevention device similar to the shape of the diaphragm to restore it to the same shape as it was in the body as much as possible, then place it in water together with the deformation prevention device, and finish it with gauze. The shape of the elbow was adjusted and left for about 24 hours to cure the silicone rubber composition.

After curing the silicone rubber composition, the liver parenchyma was removed by boiling, thoroughly washed with water, and dried at room temperature.
Vascular three-dimensional structure specimens of the liver were obtained. The specimen created was highly accurate and flexible, and would not deform or damage even if touched directly, making it highly practical for observing the internal structure.

Example 2 70 parts by weight of dimethylpolysiloxane having an average molecular formula, 3 parts by weight of dimethylpolysiloxane having an average molecular formula, 25 parts by weight of dimethylpolysiloxane having an average molecular formula, surface hexamethyldisiloxane 13 parts by weight of humid silica with a specific surface area of 200 m'/9, surface treated with
A mixture of 0.1 parts by weight of Bu2S n (OOOC15H23)2 was heat-treated at 150° C. for 3 hours to prepare composition tBl.

This composition (B1180 parts by weight contains 3 parts of dimethylpolysiloxane represented by the average molecular formula, 0H2
=OH8i (○Me) 812 parts by weight, (1-Pro)
, 0.4 parts by weight of Ti were uniformly mixed to prepare a silicone rubber composition tC1. In the same manner as in Example 1, 1 g of four types of colorants were added to each +0110 (l) of this composition, mixed uniformly, and colored into four types.

Next, these four types of silicone rubber compositions were injected into cannulae inserted into the portal vein, hepatic artery, bile duct, and hepatic vein of a human autopsy-extracted liver in the same manner as in Example 1 to prepare a liver vascular three-dimensional structure specimen. As with Example 1, this product had extremely high precision, was flexible, and did not deform or damage even when touched directly, so it was possible to observe its internal structure.

Claims (1)

[Claims] 1. Three-dimensional vascular structure specimen of an organ made of silicone rubber 2.
, the silicone rubber has A) an average compositional formula of RaSiO4,-a (where R is a monovalent dir- organic group, and a is 1.8 to 2.1), and contains aliphatic unions at both ends of the molecular chain. 100 parts by weight of linear diorganopolysiloxane containing saturated groups B) having at least two hydrogen groups bonded to silicon atoms in the molecule, and having the above A)
o for the aliphatic lower A in the diorganopolysiloxane of
, 5L5 times the amount of organohydrodiene polysiloxane containing SiH groups C) 0 to 50 parts by weight of silica filler. D) A three-dimensional vascular structure specimen 3 of a bare vessel according to claim 1 obtained from a composition comprising a catalytic amount of an addition reaction catalyst, wherein the silicone rubber has a) an average compositional formula of RbSi; 04-b (where R2 is a monovalent -r~ organic group, b is 1.8 to 2.1) Linear diorganopolysiloxane whose molecular chain ends are blocked with hydroxyl groups 100 weight Part b) Organosilicon compound having at least two hydrolyzable atoms or groups bonded to a silicon atom in the molecule 0.
1 to 20 parts by weight C Okibe Rica filler 0 to 5
The three-dimensional vascular structure specimen of an organ according to claim 1, which is obtained from a composition consisting of 0 parts by weight of a condensation reaction catalyst such as a metal acid salt or an amine compound.