JPS5917999A - ビリルビンの定量法及びその定量用試薬組成物 - Google Patents
ビリルビンの定量法及びその定量用試薬組成物Info
- Publication number
- JPS5917999A JPS5917999A JP12763282A JP12763282A JPS5917999A JP S5917999 A JPS5917999 A JP S5917999A JP 12763282 A JP12763282 A JP 12763282A JP 12763282 A JP12763282 A JP 12763282A JP S5917999 A JPS5917999 A JP S5917999A
- Authority
- JP
- Japan
- Prior art keywords
- bilirubin
- oxidase
- genus
- enzyme
- quantifying total
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 title claims abstract description 174
- 238000000034 method Methods 0.000 title claims description 44
- 239000000203 mixture Substances 0.000 title claims description 31
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 30
- 108010015428 Bilirubin oxidase Proteins 0.000 claims abstract description 47
- 238000008050 Total Bilirubin Reagent Methods 0.000 claims abstract description 34
- 108010029541 Laccase Proteins 0.000 claims abstract description 20
- 102000003425 Tyrosinase Human genes 0.000 claims abstract description 15
- 108060008724 Tyrosinase Proteins 0.000 claims abstract description 15
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 6
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 5
- 241000222511 Coprinus Species 0.000 claims abstract description 5
- 239000004094 surface-active agent Substances 0.000 claims abstract description 5
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 4
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 4
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 3
- 102000004190 Enzymes Human genes 0.000 claims description 38
- 108090000790 Enzymes Proteins 0.000 claims description 38
- 238000006243 chemical reaction Methods 0.000 claims description 33
- 238000008789 Direct Bilirubin Methods 0.000 claims description 17
- 238000012360 testing method Methods 0.000 claims description 17
- 108010024957 Ascorbate Oxidase Proteins 0.000 claims description 8
- 238000011002 quantification Methods 0.000 claims description 6
- 241000222640 Polyporus Species 0.000 claims description 5
- -1 aromatic carboxylic acids Chemical class 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 241000121220 Tricholoma matsutake Species 0.000 claims description 4
- 229940079593 drug Drugs 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 240000008067 Cucumis sativus Species 0.000 claims description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 3
- 102000035195 Peptidases Human genes 0.000 claims description 3
- 239000004922 lacquer Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 230000003213 activating effect Effects 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- 229940072107 ascorbate Drugs 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 16
- 210000002966 serum Anatomy 0.000 abstract description 10
- 239000012085 test solution Substances 0.000 abstract description 7
- 210000002700 urine Anatomy 0.000 abstract description 4
- 241001103808 Albifimbria verrucaria Species 0.000 abstract description 2
- 241000223251 Myrothecium Species 0.000 abstract description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 14
- 238000004440 column chromatography Methods 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 9
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- 229920005654 Sephadex Polymers 0.000 description 8
- 239000012507 Sephadex™ Substances 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 8
- NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 description 7
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
- 238000011088 calibration curve Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 238000005259 measurement Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000000108 ultra-filtration Methods 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 239000012521 purified sample Substances 0.000 description 4
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- 102100027211 Albumin Human genes 0.000 description 3
- 108010088751 Albumins Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 241000208225 Rhus Species 0.000 description 3
- 235000014220 Rhus chinensis Nutrition 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- GWZYPXHJIZCRAJ-UHFFFAOYSA-N Biliverdin Natural products CC1=C(C=C)C(=C/C2=NC(=Cc3[nH]c(C=C/4NC(=O)C(=C4C)C=C)c(C)c3CCC(=O)O)C(=C2C)CCC(=O)O)NC1=O GWZYPXHJIZCRAJ-UHFFFAOYSA-N 0.000 description 2
- RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- 108010059712 Pronase Proteins 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 102100033220 Xanthine oxidase Human genes 0.000 description 2
- 108010093894 Xanthine oxidase Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical group O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 210000000941 bile Anatomy 0.000 description 2
- QBUVFDKTZJNUPP-UHFFFAOYSA-N biliverdin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(C=C2C(=C(C)C(C=C3C(=C(C=C)C(=O)N3)C)=N2)CCC(O)=O)N1 QBUVFDKTZJNUPP-UHFFFAOYSA-N 0.000 description 2
- BPYKTIZUTYGOLE-UHFFFAOYSA-N billirubin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(C=C3C(=C(C=C)C(=O)N3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 229940097043 glucuronic acid Drugs 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Chemical compound OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 229960004889 salicylic acid Drugs 0.000 description 2
- 238000005185 salting out Methods 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IEORSVTYLWZQJQ-UHFFFAOYSA-N 2-(2-nonylphenoxy)ethanol Chemical compound CCCCCCCCCC1=CC=CC=C1OCCO IEORSVTYLWZQJQ-UHFFFAOYSA-N 0.000 description 1
- WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 description 1
- BDDLHHRCDSJVKV-UHFFFAOYSA-N 7028-40-2 Chemical compound CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O BDDLHHRCDSJVKV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 244000251953 Agaricus brunnescens Species 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 241000349761 Berlinia Species 0.000 description 1
- UJKPHYRXOLRVJJ-MLSVHJFASA-N CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C Chemical compound CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C UJKPHYRXOLRVJJ-MLSVHJFASA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 244000251987 Coprinus macrorhizus Species 0.000 description 1
- 235000001673 Coprinus macrorhizus Nutrition 0.000 description 1
- 102000019265 Cytochrome c1 Human genes 0.000 description 1
- 108010007528 Cytochromes c1 Proteins 0.000 description 1
- 102100028717 Cytosolic 5'-nucleotidase 3A Human genes 0.000 description 1
- 229920002271 DEAE-Sepharose Polymers 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 206010019837 Hepatocellular injury Diseases 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000003833 bile salt Substances 0.000 description 1
- 229940093761 bile salts Drugs 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229940099898 chlorophyllin Drugs 0.000 description 1
- 235000019805 chlorophyllin Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 208000009866 extrahepatic cholestasis Diseases 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 229960003569 hematoporphyrin Drugs 0.000 description 1
- 229940025294 hemin Drugs 0.000 description 1
- BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 208000001024 intrahepatic cholestasis Diseases 0.000 description 1
- 230000007872 intrahepatic cholestasis Effects 0.000 description 1
- PZLXYMQOCNYUIO-UHFFFAOYSA-N lithium;hydrochloride Chemical compound [Li].Cl PZLXYMQOCNYUIO-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002923 metal particle Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 229920000847 nonoxynol Polymers 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229940083575 sodium dodecyl sulfate Drugs 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- PQMSFAORUFMASU-UHFFFAOYSA-M sulfacetamide sodium anhydrous Chemical compound [Na+].CC(=O)[N-]S(=O)(=O)C1=CC=C(N)C=C1 PQMSFAORUFMASU-UHFFFAOYSA-M 0.000 description 1
- FDDDEECHVMSUSB-UHFFFAOYSA-N sulfanilamide Chemical compound NC1=CC=C(S(N)(=O)=O)C=C1 FDDDEECHVMSUSB-UHFFFAOYSA-N 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical class OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12763282A JPS5917999A (ja) | 1982-07-23 | 1982-07-23 | ビリルビンの定量法及びその定量用試薬組成物 |
| DE19823239236 DE3239236A1 (de) | 1982-02-18 | 1982-10-20 | Verfahren zur quantitativen bestimmung von physiologischen komponenten in biologischen fluessigkeiten oder gasen |
| DE3249743A DE3249743C2 (enrdf_load_stackoverflow) | 1982-02-18 | 1982-10-20 | |
| FR8217632A FR2521589B1 (fr) | 1982-02-18 | 1982-10-21 | Procede pour la determination quantitative de composants physiologiques dans des fluides biologiques |
| GB08230397A GB2115926B (en) | 1982-02-18 | 1982-10-25 | Method for the quantitative determination of physiological components in biological fluids |
| US06/436,385 US4554249A (en) | 1982-02-18 | 1982-10-25 | Method for the quantitative determination of physiological components in biological fluids |
| CA000414104A CA1185883A (en) | 1982-02-18 | 1982-10-25 | Method for the quantitative determination of bilirubin in biological fluids |
| IT48544/83A IT1203658B (it) | 1982-07-23 | 1983-06-21 | Metodo per la determinazione quantitativa di componenti fisiologici in fluidi biologici |
| ES524270A ES8501444A1 (es) | 1982-07-23 | 1983-07-19 | Un metodo para la determinacion cuantitativa de bilirrubina conjugada en fluidos biologicos. |
| ES534138A ES8601482A1 (es) | 1982-07-23 | 1984-07-09 | Un metodo para la determinacion cuantitativa de componentes fisiologicos en fluidos biologicos. |
| CA000464110A CA1218586A (en) | 1982-02-18 | 1984-09-26 | Method for the quantitative determination of physiological components in biological fluids |
| IT8422941A IT1213222B (it) | 1982-07-23 | 1984-10-01 | Metodo per la determinazione quantitativa di componenti fisiologici in fluidi biologici. |
| FR8419005A FR2556010B1 (fr) | 1982-02-18 | 1984-12-12 | Procede pour la determination quantitative de composants physiologiques dans des fluides biologiques |
| GB08431875A GB2152215B (en) | 1982-02-18 | 1984-12-18 | Method for the quantitative determination of physiological components in biological fluids |
| US06/749,425 US4839279A (en) | 1982-02-18 | 1985-06-27 | Method for the quantitative determination of physiological components in biological fluids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12763282A JPS5917999A (ja) | 1982-07-23 | 1982-07-23 | ビリルビンの定量法及びその定量用試薬組成物 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9993987A Division JPS62282598A (ja) | 1987-04-24 | 1987-04-24 | 総ビリルビンの定量法及び定量用試薬組成物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5917999A true JPS5917999A (ja) | 1984-01-30 |
| JPS6233880B2 JPS6233880B2 (enrdf_load_stackoverflow) | 1987-07-23 |
Family
ID=14964889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12763282A Granted JPS5917999A (ja) | 1982-02-18 | 1982-07-23 | ビリルビンの定量法及びその定量用試薬組成物 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS5917999A (enrdf_load_stackoverflow) |
| IT (1) | IT1213222B (enrdf_load_stackoverflow) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60151561A (ja) * | 1984-01-19 | 1985-08-09 | Nitsusui Seiyaku Kk | ビリルビンの定量方法 |
| JPS60154162A (ja) * | 1984-01-24 | 1985-08-13 | Nitsusui Seiyaku Kk | 遊離ビリルビンの定量方法 |
| JPS60178360A (ja) * | 1984-02-24 | 1985-09-12 | Fujimoto Daiagunosuteitsukusu:Kk | 総ビリルビンおよび直接ビリルビンの分別定量法 |
| EP0770688A1 (en) | 1995-10-27 | 1997-05-02 | Kyowa Medex Co., Ltd. | Method of quantitative determination of bilirubin and a reagent therefor |
| CN111455018A (zh) * | 2020-03-03 | 2020-07-28 | 天津大学 | 含有枯草芽孢杆菌漆酶的直接胆红素检测试剂盒 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6450880U (enrdf_load_stackoverflow) * | 1987-09-26 | 1989-03-29 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54151193A (en) * | 1978-05-19 | 1979-11-28 | Eastman Kodak Co | Bilirubin specific bacterial enzyme composition * use and production thereof |
| JPS5876100A (ja) * | 1981-10-30 | 1983-05-09 | Ono Pharmaceut Co Ltd | ラツカ−ゼの使用法 |
-
1982
- 1982-07-23 JP JP12763282A patent/JPS5917999A/ja active Granted
-
1984
- 1984-10-01 IT IT8422941A patent/IT1213222B/it active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54151193A (en) * | 1978-05-19 | 1979-11-28 | Eastman Kodak Co | Bilirubin specific bacterial enzyme composition * use and production thereof |
| JPS5876100A (ja) * | 1981-10-30 | 1983-05-09 | Ono Pharmaceut Co Ltd | ラツカ−ゼの使用法 |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60151561A (ja) * | 1984-01-19 | 1985-08-09 | Nitsusui Seiyaku Kk | ビリルビンの定量方法 |
| JPS60154162A (ja) * | 1984-01-24 | 1985-08-13 | Nitsusui Seiyaku Kk | 遊離ビリルビンの定量方法 |
| JPS60178360A (ja) * | 1984-02-24 | 1985-09-12 | Fujimoto Daiagunosuteitsukusu:Kk | 総ビリルビンおよび直接ビリルビンの分別定量法 |
| EP0770688A1 (en) | 1995-10-27 | 1997-05-02 | Kyowa Medex Co., Ltd. | Method of quantitative determination of bilirubin and a reagent therefor |
| US5858695A (en) * | 1995-10-27 | 1999-01-12 | Kyowa Medex Co., Ltd. | Method of quantitative determination of bilirubin and a reagent therefor |
| CN111455018A (zh) * | 2020-03-03 | 2020-07-28 | 天津大学 | 含有枯草芽孢杆菌漆酶的直接胆红素检测试剂盒 |
Also Published As
| Publication number | Publication date |
|---|---|
| IT8422941A0 (it) | 1984-10-01 |
| IT1213222B (it) | 1989-12-14 |
| JPS6233880B2 (enrdf_load_stackoverflow) | 1987-07-23 |
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