JPS591687B2 - Serum cholesterol increase inhibitor - Google Patents

Description

[Detailed description of the invention] TECHNICAL FIELD The present invention relates to a substance that suppresses elevation of serum cholesterol.

Conventionally, it has been known that foods containing many indigestible components such as pectin, konjac, and mannan suppress the rise in serum cholesterol, and various so-called grueling fibers have been introduced in the literature as substances that suppress the rise in serum cholesterol. However, it includes things that are not necessarily recognized as effective.

In particular, rice bran is not effective as it is, and the present inventor has previously reported in Japanese Patent Application No. 54-49722 that a substance containing cellulose, hemicellulose, and lignin as main components extracted from rice bran is effective. I found out.

The present invention is related to this, and as a result of various studies, it has been confirmed that hemicellulose is particularly effective among the three components, and by extracting only hemicellulose from defatted rice bran using a conventional method, it is extracted from serum cholesterol. It is characterized in that it is used as a substance that suppresses the rise.

Next, the method for extracting the serum cholesterol increase suppressing substance of the present invention from rice bran is generally carried out as follows.

That is, defatted rice bran is obtained by a conventional method, and this is used as a raw material, and after the starch is removed by the action of a starch-degrading enzyme, the residue is treated with an alkaline aqueous solution to dissolve and extract the hemicellulose fraction. Neutralize the hemicellulose extract and remove the protein precipitate, neutralize the resulting separated liquid and add an alcohol solvent to obtain a hemicellulose precipitate, separate it, dissolve it in water, and add to this aqueous solution. A dialysis treatment is performed, and the dialysate is dried to obtain a serum cholesterol increase-inhibiting substance consisting of purified hemicellulose.

Furthermore, one example of the extraction method will be explained in detail.

Commercially available defatted rice bran is used as a raw material, and an appropriate amount of 0.1% glycoamylase solution is added to it, for example, 5 g to 100 g of rice bran.
The mixture is mixed at a ratio of 100 t and incubated at 40° C. for 24 hours to decompose starch, which is then filtered to obtain a residue.

The residue was further washed with water and filtered, and 0.5N sodium hydroxide was added to the resulting residue, which was then filled with N2 gas and shaken at room temperature for 18 hours to extract the alkali-soluble hemicellulose fraction.

Next, this was centrifuged to remove the residue, and the supernatant was neutralized with glacial acetic acid, and 5% trichloroacetic acid was added.
Proteins were precipitated.

This was centrifuged to separate and remove the protein, and the resulting supernatant was neutralized with sodium hydroxide, and then about 4 times the amount of ethanol was added and aged for 12 hours.

After sufficient hemicellulose has been precipitated in this way, it is centrifuged to separate the precipitate, which is dissolved by adding an appropriate amount of distilled water.The aqueous solution is dialyzed in running water using a cellophane tube. Freeze-drying gave pale yellow hemicellulose.

About 5% (by weight) of hemicellulose is obtained from defatted rice bran.

The hemicellulose thus obtained was subjected to a rat feeding test as described below, and it was confirmed that the hemicellulose had a significantly high effect of suppressing the increase in serum cholesterol.

The experimental animals were Wistar male rats weighing 1,201 kg, which were pre-fed for 7 days on a standard diet.
The following experimental feed was administered to each group for 8 days.

Each rat was placed in a wire mesh cage divided into 6 rooms and raised in a breeding room at a room temperature of 22±1°C.

The composition of the experimental feed was that commonly used in cholesterol metabolism tests, and the following four types of feed were given to the four groups described above.

Control feed: Feed according to the invention, which mainly consists of sucrose and casein, and contains 1% cholesterol and 0.25% bile acid I: Feed according to the invention, in which part of the carbohydrates in the control feed is replaced with 2% hemicellulose Feed according to the invention ■ : A feed in which part of the carbohydrates in the control feed was replaced with 0.5% hemicellulose.NDF feed: A feed in which part of the carbohydrates in the control feed was replaced with *5% NDF *NDF is hemicellulose extracted from defatted rice bran.
It is mainly composed of cellulose and lignin, and is a serum cholesterol increase control substance according to the invention of Japanese Patent Application No. 54-49722 filed earlier.

The rats reared in this way were subjected to blood sampling, and after laparotomy, serum cholesterol and liver cholesterol were measured using conventional analytical methods, and the results shown in the table below were obtained.

As is clear from the above table, the hemicellulose-added feeds ① and ② of the present invention have a remarkable effect on suppressing the rise in serum cholesterol compared to the control feed, and are even more excellent in suppressing the rise in serum cholesterol than the NDF feed. This confirmed that hemicellulose in NDF is particularly effective.

As a result of examining the significant difference through stochastic processing of this experimental data, it was found that there was a significant decrease with a small risk rate of 5%.

As described above, the present invention is extremely effective when hemicellulose extracted from defatted rice bran is used as a substance for suppressing elevation of serum cholesterol.

Claims (1)

[Claims] 1. A serum cholesterol increase-inhibiting substance made from defatted rice bran and made from hemicellulose separated from it.