JPS59152317A - Composition for oral cavity - Google Patents

Abstract

PURPOSE:A composition for oral cavity useful as toothpaste, lubricating dentifrice, liquid dentifrice mouthwash, paste, etc., obtained by using mutanase (a drug for preventing decay of the teeth) together with casein, casein hydrolyzate or its mixture so that mutanase is stably blended with the composition. CONSTITUTION:0.1-5wt%, preferably 0.3-3wt% casein, such as casein hydrolyzate obtained by hydrolyzing milk casein with an enzyme derived from pancreas or trypsin or its mixture as a stabilizer for mutanase based on total amount of composition is blended with a mutanase-containing composition for oral cavity, to give a composition for oral cavity keeping sufficient enzymatic activity of mutanase for a long period, showing improved preventing effect on decay of the teeth. The composition can be further blended with common components, especially with an N-acylsarcosine salt alone or with a surcrose fatty acid ester as a foaming agent to improve further stability of mutanase.

Description

[Detailed description of the invention] Toothpaste containing stable mutanase, which is a medicinal agent for preventing caries, such as 4) °] made toothpaste, powdered toothpaste, cleaning agent 1), denture cleaning agent, etc. Related to oral compositions.

Prior art It has been known that one of the mechanisms of dental caries development is that Streptococcus mutans, a caries-causing bacterium, converts sucrose into a water-insoluble viscous Mt' that has an α-1,3-glucoside bond. It is said that Mutan, a glucan of 4: is produced, and it firmly adheres to the tooth surface, where it generates lactic acid and demineralizes the tooth (Clinic and Bacteria, Vol. 1, 2, p. 1, 1974). . Mutanase is α-1,3 of this mukun.
-It is an enzyme that breaks down glucoside bonds, and its action is used to break down mutan, and -7 it is being tested to prevent the adhesion of Treptococcus mutans to the tooth surface, thereby preventing dental caries. (Journal of Dental Research, Vol. 51, Suppl.-394
Page, 1972). It has also been proposed that mutanase be added to toothpaste, etc.
No. 6 1-mutanase has been shown to be effective in preventing dental caries.

However, mutanase is very unstable, and when it is blended into an oral composition, it tends to lose its enzymatic activity due to the influence of other ingredients, and so far, no oral composition has been found that stably contains mutanase.

Purpose of the Invention In view of the above circumstances, the inventors, Kazuki, and his colleagues conducted intensive research to obtain an oral composition containing mutanase in a stable manner.
It was discovered that the composition of the invention (i.e., the oral composition of the invention) was found to uniquely stabilize mutanase in an oral composition. - Mutanase - Casein, casein 1Jll as its calcifying agent
4. Containing a mixture of water decomposition products and C.
] 1 symptom. Inventively, in the Kagawa silk composition, the blended tanase maintains sufficient enzyme occupancy for a long period of time.
+1 Shi, effective in preventing tooth decay.

Casein or casein hydrolyzate, which is used as a mutanase stabilizer in the invention, can be any one that can be dried by drying. You may. casein hydrolyzate) i (expressed τλ) As an example, milleccasein is pancreatic 11i!
, Hydrolyzed with Yamaki's enzyme or 1-ribusin (for example, commercially available 1-lipunakenubef0ton (US B, +3 I-(ZL 4j'! 1-
Nin Suibeno (manufactured by Isui Seiyaku Co., Ltd.), Kyokuto Peptone (manufactured by Kyokuto Co., Ltd.) and Mikunipep (manufactured by Mikuni Co., Ltd.), and the like can be used. In terms of the stabilizing effect of casein, casein hydrolyzate or a mixture thereof, mutanase and stability of the resulting composition, etc.
0.3 to 5% of the total amount of the composition (weight @ section, hereinafter)
- Preferably, 0.3 to 3 ratios are mixed.

The mutanase may be any commonly available one, such as Trichoderma harzianum OMZ779, Cladosporium regine QM7998, Leptomycenu veerensinu Aspergillus niF rannus, Flavobacterium N.B., Pseudomonas N. The mutanase-producing bacteria of Bii are cultured in a soil containing a normal nutrient source or a soil to which α-1,3-glucan is added, and the supernatant is collected using salting-out method, adsorption method, solvent fractionation method, etc. The properties of mutanase C1 and its optimal 1) II are generally on the acidic side, and they decompose mutan and reduce it. It has the effect of liberating and solubilizing sugar.

In the present invention, usually 500
By blending ~100,000 units/g of mutanase (1,1 to 20%), the caries preventive effect can be achieved. Note that the mutanase 1.11'1 position is -(1,1M j
”+S+ l Sunwa 7, I!lijsa (pT-15,7)
Medium, 40°C and boil for 1 minute.
It is defined as an enzyme that liberates reducing sugars of IItM in terms of glucone.

The uninvented ``knee compound acid'' can be added to toothpaste-4゛・in accordance with the conventional method.
1. Can be made into a net shape for liquid toothpaste, mouthwash, Vanuta, denture cleaner, etc. (The ingredients in I!! are not limited to J Samurai, and may include those normally used in the composition of this scale - for example - Toothpaste C])
I1, diphosphoric acid calci cum, j fire drink calciuno -
(dt
14i agent - wetting agent such as glycerin and sorbitol -
N-acyl sarcosine salt - sucrose fatty acid ED/I/flIf acid salt, foaming agent such as algyl jetanolamide, carboxymethyl cellulone lithium, carrageenan - sodium polyacrylate, binder of xanthin gum nat - IJ flavoring agent -Fragrance fj can be blended. In addition, (υ1) When using aluminum hydroxide as a toothpaste, the stability of mutanase is further improved by removing sorbitol as a wetting agent. Foaming agents (interface 7
11. As a lingual agent, N-acylsarcosine Kamifuku can be used alone or in combination with sucrose fatty acid ether to stabilize mutanase. It is possible to improve the balance.

The N-acyl sarcosine salt has the formula: % (in the formula, 1 or an allenyl having 11 to 17 carbon atoms) HI
J-M stands for Potassium I/Rium Alkali 4
meaning 1+17 ammonium], such as -N-lauroyl salt: 1 thinner lium-N-mil7 toyl sarcosinate sodium, N-valemitoyl sarcocinnerium, N-
y, tearoylsarcocinnatium-1-lium sodium, and it is preferable to mix it in a ratio of 0.1 to 3.0 parts with respect to the total amount of the combined acid.

As a sucrose fatty acid ester, the number of carbon atoms in the fatty acid residue is 1.
Those derived from higher fatty acids of 0 to ]8, and those mainly composed of monoesters are preferable, for example, -1 old plate D K ester F-50-F-'to-F-90, l='-110
, I'-] 40-F-160 (formerly manufactured by Dai-Kogyo Seiyaku Co., Ltd.) Contains a fatty acid (carbon number: O to 18) from hardened beef tallow as a fatty acid component, and the monoester content is 3 (L4.0.45.5 (L 60-70) -
DK ester L-13+Ht, manufactured by T-Gyo Seiyaku Co., Ltd., sucrose lauric acid ester], and the like, which are blended in a ratio of 702 to 100% of the first acid compound J Zenko.

The knee composition of the present invention may further contain monofluorophosphate, chlorhexidine, an anti-inflammatory agent, and other medicinal agents.

The results of testing the effect of the invention on stabilizing knase (casein and O-casein hydrolyzate) are shown.

Mutanase A wired toothpaste containing various proteins and protein hydrolysates according to the /r recipe was manufactured.In addition, as a control, tatashi-protein or protein hydrolyzate with the same formulation was prepared. A wire toothpaste containing mutanase was produced.

Toothpaste prescription ingredients Protein-related protein or protein hydrolyzate + 0.5 (
(See Table 1) Sodium N-lauroyl sarcosine O5 Sucrose laurate Entel 15 Calcium phosphate 50.0 Norbyl
20.0 carrageenan
1゜Zatsukarinna 1~Rium
o, 15 incense old
1.0 Mutanase 1o unit/g Toothpaste water Adjusted to 100% After storing these toothpastes at 42℃ for 100 days - The activity of mutanase remaining in the toothpaste (free reducing sugars by Nmogy Nelson method) was determined. The residual rate (%) was determined by comparing the activity after production 1 (1).
Show the front number.

As shown in Table 751, among each protein, even when gelatin and albumin were added, the activity of mutanase remained the same as the control, while casein's survival rate was 4.
:, +I' differentially stabilizes mutanase, and even -effects of activating mutanase have been observed. Also-
In each <IH white hydrolyzate, casein hydrolyzate power'' IIyC stabilizes mutanase and adds tif
It is clear that it has become I.

EXAMPLES The present invention will be explained in more detail with reference to Examples.

Example 1 Seven toothpastes were manufactured according to the conventional method using the following formulation.

Ingredients Calcium diphosphate 45.0 Sorbitol 250 Carpoquin methylcellulone 1-lium 1ON-Lauroylsarcosine sodium 05 Sucrose fatty acid ester (1.5 fatty acid from hardened beef tallow, monoester Tokoyo 70) Karin sodium 02 Kobetsu 1.0 Zein hydrolysis product 05 Mutanase (about 10,000 Li/g) 02 Water
Adjustment to 100% Example 2 A toothpaste was manufactured according to the conventional method according to the following recipe.

Ingredients Aluminum hydroxide 40.0 Norbyl 300 Carrageenan 1ON-, Uroylsarcocinna I.Rium 05 Sucrose laurate 1.0 Zasocalin sodium 11um 02-Kosai゛1

1. .

Case'in 10
Tanase (20,000 units/gl 0
．． Adjustment to 37K 100% Example 3 A moist toothpaste was manufactured using the conventional method according to the following recipe.

Ingredients Calcium diphosphate 45.01
Ji Jl! #force lesium 2
5.0 Glycerin ]0. O
Sodium lauryl sulfate 1.0 Sodium phosphate 1.0 Zatukarin sodium 0.2 Flavoring
1.0 Zein hydrolyzate 0.6 Mutanase [
1000 units/fl) 1.5 water
Adjustment to 100 pieces Example 4 Maunu otshu was produced according to the conventional method according to the following recipe.

Ingredients

Claims (1)

[Claims] (1) A casein-casein hydrolyzate or a mixture thereof is blended as a mutanase stabilizer in the 1] cavity silk composition Jc containing mutanase fA-
A ready-to-use oral preparation with special characteristics.