JPS5914184B2 - How to move liquid samples - Google Patents
How to move liquid samplesInfo
- Publication number
- JPS5914184B2 JPS5914184B2 JP54026846A JP2684679A JPS5914184B2 JP S5914184 B2 JPS5914184 B2 JP S5914184B2 JP 54026846 A JP54026846 A JP 54026846A JP 2684679 A JP2684679 A JP 2684679A JP S5914184 B2 JPS5914184 B2 JP S5914184B2
- Authority
- JP
- Japan
- Prior art keywords
- sample material
- chamber
- duct
- inlet
- outlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Description
【発明の詳細な説明】
本願発明は、液体サンプルを測定室に少量づつしかも乱
れをおこさすに供給するための方法に関するものである
。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for supplying a liquid sample to a measurement chamber in small quantities and in a turbulent manner.
５ この室には入口および出口ダクトが備えられており
、測定キユベツト内の分散分子のミクロ観察、例えば電
気泳動測定の際に使用される。5 This chamber is equipped with inlet and outlet ducts and is used for microscopic observation of dispersed molecules in the measurement cube, for example in electrophoretic measurements.
前述したような測定結果を得るためには、サンプル物質
で分散される無数の分子が同一の測定条１０件にて観察
されねばならないが、測定装置の特性かられずかの分子
しかサンプル物質の一位置において得ることができない
。In order to obtain the above-mentioned measurement results, countless molecules dispersed in the sample material must be observed under the same 10 measurement conditions, but due to the characteristics of the measurement device, only a small number of molecules can be detected as part of the sample material. Can't get in position.
一度に多数の分子を受け入れるためには、そのサンプル
物質をわすか移動させねばならない。公知の装置では、
バルブあ１５るいはコックを同時に開け閉めすることで
サンプル物質を動かしている。この動かすエネルギーは
静水圧によりもたらされる。他の公知の装置ではポンプ
によつて新しいサンプル物質を供給することによつて分
子を置き換えクｏ ている。To accommodate large numbers of molecules at once, the sample material must be shunted or moved. In known devices,
The sample material is moved by simultaneously opening and closing valves or cocks. The energy for this movement is provided by hydrostatic pressure. Other known devices replace molecules by supplying fresh sample material via a pump.
し力化この両装置は不利である。なぜならば周囲とのシ
ステムのシールおよび密閉に関すする非常に厳しい要求
を満足させねばならない構成部が非常に摩滅してしまい
、それ故に温度、聞値、導電率等の物理特性が多少異る
新しいサンプラ５ ル物質が測定セルに送られてしまう
。さらにまた、それらはサンプル物質を二、三マイクロ
メータごとに再生可能に動かすことができない。そこで
本願発明は前記した諸欠点を除き、一定のサンプル物質
の高精密測定を可能とするもので３０あり、又、測定室
内のサンプル物質を非常にわずかの長さ動かせそして試
験するサンプル物質と新らしいサンプル物質とを隔離さ
せるためにバルブやコックのような機素を必要とせすに
新しいサンプル物質が室に侵入するのを防ぐための方法
を提？５ 供せんとするものである。Both devices are disadvantageous. This is because the components, which have to meet very stringent requirements regarding the sealing and hermeticity of the system with its surroundings, become very worn out and therefore new components with slightly different physical properties such as temperature, thermal conductivity, conductivity, etc. Sample material is sent to the measurement cell. Furthermore, they cannot reproducibly move the sample material every few micrometers. Therefore, the present invention eliminates the above-mentioned drawbacks and makes it possible to perform high-precision measurements on a certain sample material.It also enables the sample material to be moved a very small distance within the measurement chamber and is then able to move the sample material to be tested. Is there a way to prevent new sample material from entering the chamber without requiring elements such as valves or cocks to isolate it from new sample material? 5.It is intended to be offered as an offering.
さらに本願発明は、測定中に層流を確保できるようにす
ることを目的とするものである。Furthermore, the present invention aims to ensure laminar flow during measurement.
ウ【一
本願発明は、人ロダタトおよび出口ダクトを備えた測定
室内で液体サンプル物質を少量づついかなる乱れをおこ
さずに動かす方法に関するものであり、その入口ダクト
と出口ダクトが閉じられておりそして測定室の入口およ
び出口のそれぞれに結合し逆方向に作用する一対のピス
トン方式により連結されたダクトシステム内をサンプル
物質が移動することを特徴とするものである。[1] The present invention relates to a method for moving a liquid sample substance in small quantities without causing any disturbance in a measuring chamber equipped with a human body and an outlet duct, the inlet and outlet ducts being closed and the measuring chamber being It is characterized in that the sample material moves through a duct system connected by means of a pair of pistons connected to the inlet and outlet of the chamber and acting in opposite directions.
次に図面に示す二つの実施例に従つて説明する。Next, two embodiments shown in the drawings will be explained.
第１図において、ダクトシステム８内には、入口ダクト
および出口ダクトにそれぞれ開閉機素１が図示してない
外部システムに対してこのシステムを開閉するために設
けられている。このダクトシステム８内であり且つこの
二つの機素１間には、測定室２、望ましくはキユベツト
が設けられている。電極セル３がサンプル物質に電圧を
印加するために室２の前後に配置されている。このダク
トシステム８の枝部は二つのシリンダー４が共通軸に対
称に接続されている。このシリンダーの内径は等しく構
成されている。各シリンダー４内には、それぞれピスト
ン５が相互に電気的に絶縁され且つロツド６にて連結さ
れて配されている。ピストンの前面はサンプル物質と接
触する。素子７はシリンダー４の壁とピストン５との気
密シールである。In FIG. 1, in the duct system 8, switching elements 1 are provided in the inlet duct and the outlet duct, respectively, for opening and closing the system to an external system (not shown). Within this duct system 8 and between the two elements 1, a measuring chamber 2, preferably a cuvette, is provided. Electrode cells 3 are placed before and after the chamber 2 for applying a voltage to the sample material. This branch of the duct system 8 has two cylinders 4 connected symmetrically around a common axis. The inner diameters of the cylinders are made equal. Pistons 5 are arranged in each cylinder 4 and are electrically insulated from each other and connected by a rod 6. The front side of the piston is in contact with the sample material. Element 7 is a hermetic seal between the wall of cylinder 4 and piston 5.
シートレバー９は連結ロツド６にＡでヒンジされたホー
ク部によつてピストン５を作動させる。室２およびシリ
ンダー４を有するダクトシステムに機素１の作動で液体
サンプル物質が満されると、電気泳動分析を始めること
ができる。サンブル物質はレバー９でピストン５の同時
変位を行うことにより室２の中で動かされる。第２図に
示される別の実施例では、ピストン５がダクトシステム
８のふくらみ部１０内でサンプル物質に作用する。ピス
トン５間の移動方向へのしつかりした連結はナツト１１
と噛み合つているところの連結素子のスピンドル６によ
り具体化されている。ボールベアリング１２にシートさ
れているナツト１１を回転するとサンプル物質が動かさ
れる。たびたび特別な必要からチユーブシステムの密封
が要求される。その場合はピストンドライブが完全にシ
ールされコイルを備えたナツト１１が外部の磁石１３で
動かされる。The seat lever 9 operates the piston 5 by means of a fork hinged at A to the connecting rod 6. Once the duct system with chamber 2 and cylinder 4 has been filled with liquid sample material by actuation of element 1, electrophoretic analysis can begin. The sample material is moved within the chamber 2 by simultaneous displacement of the piston 5 with the lever 9. In another embodiment shown in FIG. 2, a piston 5 acts on the sample material within a bulge 10 of a duct system 8. A tight connection between the pistons 5 in the direction of movement is provided by a nut 11.
It is embodied by the spindle 6 of the coupling element, which is in mesh with the coupling element. Rotating a nut 11 seated on a ball bearing 12 moves the sample material. Special needs often call for hermetically sealed tube systems. In that case the piston drive is completely sealed and the nut 11 with the coil is moved by an external magnet 13.
第１図は、第１の実施例の概略図。
第２図は、第２の実施例の概略図。１・・・・・・開閉
機素、２・・・・・・測定室、３・・・・・・電気セル
、４・・・・・・シリンダ、５・・・・・・ピストン、
６・・・・・・ロツド、７・・・・・・シーリング、８
・・・・・・ダクトシステム、９・・・・・・レバー
１１・・・・・・ナツト、１２・・・・・・ベアリング
、１３・・・・・・磁石。FIG. 1 is a schematic diagram of a first embodiment. FIG. 2 is a schematic diagram of a second embodiment. 1...Opening/closing element, 2...Measurement chamber, 3...Electric cell, 4...Cylinder, 5...Piston,
6... Rod, 7... Sealing, 8
...Duct system, 9...Lever
11... Nut, 12... Bearing, 13... Magnet.
Claims (1)
おり、この室の入口および出口に接続したダクトシステ
ム内でサンプル物質が、反対方向を向き相互がしつかり
と結合された一対のピストン方式で動かされることを特
徴とする入口ダクトおよび出口ダクトを備えた測定室内
で少量づつ乱れを起こさずに液体サンプル物質を動かす
方法。1 The inlet and outlet ducts of the measuring chamber are closed and the sample material is moved in the duct system connected to the inlet and outlet of this chamber by means of a pair of pistons facing in opposite directions and tightly connected to each other. A method for moving a liquid sample substance in small quantities and without disturbance within a measurement chamber having an inlet duct and an outlet duct, characterized in that
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD0WPGN/204118 | 1978-03-10 | ||
| DD20411878A DD137975B1 (en) | 1978-03-10 | 1978-03-10 | PROCESS AND ARRANGEMENT FOR FEEDING FLUID SAMPLING |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54151491A JPS54151491A (en) | 1979-11-28 |
| JPS5914184B2 true JPS5914184B2 (en) | 1984-04-03 |
Family
ID=5511781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP54026846A Expired JPS5914184B2 (en) | 1978-03-10 | 1979-03-09 | How to move liquid samples |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS5914184B2 (en) |
| DD (1) | DD137975B1 (en) |
| DE (1) | DE2905119A1 (en) |
| GB (1) | GB2017755B (en) |
| IN (1) | IN151280B (en) |
| SE (1) | SE440827B (en) |
| SU (1) | SU957044A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2468120B1 (en) * | 1979-10-17 | 1981-11-20 | Inst Nat Sante Rech Med | MEASURING CELL AND AUTOMATIC ANALYTICAL MICROELECTROPHORESIS DEVICE |
-
1978
- 1978-03-10 DD DD20411878A patent/DD137975B1/en unknown
-
1979
- 1979-02-10 DE DE19792905119 patent/DE2905119A1/en active Granted
- 1979-02-13 SU SU797770445A patent/SU957044A1/en active
- 1979-03-09 JP JP54026846A patent/JPS5914184B2/en not_active Expired
- 1979-03-09 SE SE7902147A patent/SE440827B/en not_active IP Right Cessation
- 1979-03-09 GB GB7908343A patent/GB2017755B/en not_active Expired
- 1979-05-29 IN IN550/CAL/79A patent/IN151280B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GB2017755A (en) | 1979-10-10 |
| DD137975A1 (en) | 1979-10-03 |
| DE2905119C2 (en) | 1987-04-23 |
| IN151280B (en) | 1983-03-19 |
| GB2017755B (en) | 1982-08-25 |
| SE440827B (en) | 1985-08-19 |
| DD137975B1 (en) | 1981-05-27 |
| SE7902147L (en) | 1979-09-11 |
| DE2905119A1 (en) | 1979-09-20 |
| JPS54151491A (en) | 1979-11-28 |
| SU957044A1 (en) | 1982-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110487645B (en) | Miniature temperature-control unsaturated soil triaxial tester and method suitable for industrial CT scanning | |
| US2833151A (en) | Device for delivering measured quantities of gases or vapours | |
| BR8203888A (en) | ARMOR WITH A DEVICE FOR MEDICATION AND / OR ANALYSIS OR SIMILAR MEDIA FLOWING THROUGH A PIPE | |
| JP6208378B2 (en) | Capillary electrophoresis device | |
| CN105486811B (en) | Test device and method of testing for gas sensing device fast response characteristic | |
| US4128456A (en) | Suction electrode for intracellular potential measurement | |
| US3787026A (en) | Chromatography valve | |
| BRPI0616017A2 (en) | apparatus and method of processing a sample of biological material | |
| JPS5914184B2 (en) | How to move liquid samples | |
| US4177667A (en) | Quick response humidity chamber | |
| US4175233A (en) | Flow cell and method for continuously monitoring deposits on flow surfaces | |
| CN110987645A (en) | Rigid-flexible composite true triaxial loading device for solving stress concentration and soil extrusion problems | |
| US3236095A (en) | Liquid inspection system and apparatus | |
| US2951745A (en) | Liquid proportioning pump system | |
| EP0248891A1 (en) | Leak detector | |
| US5474276A (en) | Valve for control of a branch line | |
| CN220207581U (en) | Underground water field index detection device | |
| SU991258A1 (en) | Plant for investigating end friction pair corrosion mechanical wear | |
| CN205015317U (en) | New ration ring quantitative system | |
| JPS585960Y2 (en) | fluid sample collection device | |
| US11999932B2 (en) | Microfluidic control | |
| US3320793A (en) | Liquid propellant piezometer | |
| US20210024866A1 (en) | Microfluidic control | |
| KR20000055827A (en) | Apparatus for Extraction Gas Collection Using Plunger | |
| IT202000005014A1 (en) | KIT FOR VOLUMETRIC MEASUREMENTS OF GAS ADSORPTION |