JPS5910619Y2 - Chromatograph data processing equipment - Google Patents

Description

[Detailed description of the invention] This invention relates to a chromatograph data processing device that calculates the area of a chromatogram obtained from a gas chromatograph and analyzes each fraction contained in a sample to be measured. An object of the present invention is to provide a chromatographic data processing device that can accurately separate the two halves of a chromatogram that is incompletely separated and output.

In a chromatograph (analyzer), as is generally known, each component contained in a sample to be measured is provided with a time difference using a column, and the time difference is applied to the detector, and the chromatogram obtained from the detector is The area of the area is calculated and the content of each bokumin is obtained from the area value.

By the way, if the sample to be measured contains two fractions whose properties are similar to the one contained in the sample, the force ram will not be able to provide a sufficient time difference between the two fractions.
For this reason, a chromatodulum in an incompletely separated state as shown in FIG. 1 is output.

Conventionally, two methods have been considered for separating two portions from such an incompletely separated chromatogram: a vertical separation method and a tangential skim method.

As shown in Figure 2, the vertical separation method detects the position of the valley C that occurs between the chromatograms A and B on one side and the other, sets a vertical line L at the position of this valley C, and then It refers to a method in which the area of two chromatograms A and B is divided into two by a vertical line L, and separated into a certain amount of each.

In addition, the tangent skim method uses the valley C as shown in Figure 3.
This refers to a method in which a tangent line D is set from the position of , and the two precepts are separated by this tangent line D.

Incidentally, the most ideal method for the actual separation line is to use a line E corresponding to the tail of the chromatogram A, as shown in FIG.

Therefore, according to the conventional vertical separation method, the area of the portion S1 shown in FIG. 4 occurs as an error.

Further, when using the tangent skim method, the area of the portion S2 surrounded by lines E and D occurs as an error.

If the line E corresponding to the tail of chroma 1 to gram A can be determined in this way, accurate separation can be performed.

However, the current situation is that it is difficult to find the line E corresponding to the hem, and we have no choice but to rely on the method described above.

The purpose of this invention is to provide a chromatographic overnight processing device that can separate the area of an incompletely separated chromatogram by a line corresponding to the tail line of the chromatogram on the side of a large peak.

This device memorizes the position (time) of the valley for two certain minutes of the incompletely separated and output chromatodulum, and also uses standard samples with known high and low concentrations only for the certain minutes that output large peaks. Obtain two chromatograms with different peak values by A proportional calculation is performed using the two area values separated at the valley position of the chromatogram obtained by the sample, and the two areas are separated by a line corresponding to the bottom line of the chromatogram having a large peak. It is something to do.

An embodiment of this invention will be described below in detail with reference to the drawings.

First, the arithmetic processing of the data processing device according to this invention will be explained.

First, a sample to be measured is placed in a chromatograph and a chromatogram is obtained.

It goes without saying that the chromatogram obtained in this case is a chromatogram in the state of incomplete separation as explained in FIGS. 1 to 4.

The position of valley C in the chromatogram to be measured in this incompletely separated state is detected.

The position of this valley C is defined by the time tyo (see FIG. 4) from the time the sample is introduced until the valley C is detected.

Next, prepare a standard sample with two concentrations, high and low, with a known concentration equal to the strength of chromatogram A (see Figures 1 to 4), which has a large peak in the chromatogram obtained by incomplete separation. A single chromatogram AH and AL as shown in FIGS. 5 and 6 having two peaks, high and low, given to the chromatogram is obtained.

These single chromatograms AH and AL are preferably the highest and lowest measurable concentrations.

These two chromatograms AH and AL are divided into two at the position corresponding to the previously detected valley C, and the area value of the two halves is Aamax. A'amax and Aamin and A'am
Each in is stored as a standard area value.

This standard area value and the position of the valley are used for all previous and subsequent measurements when the sample to be measured has a predetermined content, such as a fluid at a fixed position in a process.

Here, the sample to be measured is fed to a chromatograph to obtain its chromatogram.

If the area of the precept with a large peak point obtained by the vertical separation method of this chromatogram is designated as Aa as shown in Figure 7, and the area of a certain portion with a small peak point is designated as Ab, then the true area as shown in Figure 8 is The area values A3 and Ab can be determined by proportional calculations as shown in the following equations.

In this way, four standard area values are obtained from two single chromatograms AH and AL with different concentrations, and by performing proportional calculations using these four standard area values, a chromatogram A of the precept with a large peak is obtained. This is equivalent to separating the two parts by a line E (see Fig. 4) corresponding to the tail of the equation, and a highly accurate analysis can be performed.

An example of a device that performs the above data processing will be explained using FIG. 9.

In FIG. 9, 1 is a chromatograph.

This chromatograph 1 outputs various chromatograms as shown in FIGS. 1 to 8.

2 is a data processing device that calculates the area value of the chromatogram.

This data processing device 2 includes a means 3 for integrating the area of the chromatodarum, a means 4 for storing the time tyo from detection of the valley C and input of the sample to the time of detection of the valley C, that is, the position of the valley C, and a standard area 1,000 steps 5 for storing values Aamax and A'amax, and standard area value Aamin. A'amin
It consists of a means 6 for storing , and a tenth calculation stage 7 for performing the above-mentioned proportional calculation, and a separated area value can be obtained from the one thousand calculation stages 7.

Such a data processing device 2 can be realized by, for example, a microprocessor.

As explained above, according to this invention, when a certain portion of the sample to be measured is determined in advance, a standard area value is calculated using a standard sample having a known concentration of that certain portion, the value is memorized, and the standard area is Since the area of the incompletely separated chromatogram obtained by the sample to be measured is separated by performing proportional calculation based on the values, highly accurate area separation can be performed and highly accurate analysis results can be obtained.

The effect is extremely large in practical use.

[Brief explanation of drawings]

FIG. 1 is a curve diagram for explaining a chromatogram in an incompletely separated state output from a chromatograph, and FIGS. 2 and 3 are curve diagrams for explaining a conventional area separation method.
FIG. 4 is a curve diagram for explaining the error caused by the conventional area separation method, FIGS. 5 and 6 are curve diagrams for explaining the standard area value used in the device of this invention, and FIGS. FIG. 8 is a curve diagram explaining the data processing method executed by the device of this invention, and FIG. 9 is a system diagram showing one embodiment of the device of this invention. 1: Chromatograph, 2: Data processing device, 3: Means for calculating the area of the chromatogram, 4: Means for detecting the valley and storing the detection time point, 5, 6: Means for storing the standard area value, 7: calculation means.

Claims (1)

[Scope of Claim for Utility Model Registration] A means for obtaining an area value of a chromatogram output from a chromatograph, a valley detection means for detecting a valley between two incompletely separated adjacent peaks on the chromatogram, and a sample. These two chromatograms are obtained by using a means for storing the time from input to the detection point of the valley, and two chromatograms obtained using standard samples with different concentrations for the fraction of the larger peak of the two peaks mentioned above. When Durham is divided into two at the time of detection of the above-mentioned valleys, the areas Aamax and Aamin on the side including the peak point, respectively, and the areas Aa'max and Aa' on the side not including the peak point, respectively.
one area value Aa and the other area value Ab which are divided into two at the time of detection of the valley of the chromatodarum obtained by the sample to be measured;
Data of a chromatograph equipped with a means for calculating the true area value A0 of the bokum with a large peak point and the true area value Oa and Ab of the bokum with a small peak point by calculating the two stored values. Processing equipment.