JPS5878582A - Apparatus for enzymic reaction - Google Patents

Abstract

PURPOSE:To carry out the enzymic reaction continuously, by using an endless fibrous bundle having an immobilized enzyme. CONSTITUTION:A fibrous bundle 1 entering at a fibrous bundle entering inlet 31 of a contacting bath 3 is brought into contact with a substrate solution jetting from a showering piping 34 and directed from a fibrous bundle outlet 32 to the downward J-shaped reaction tank 4 while impregnated with the substrate solution therein. The substrate solution is subjected to the enzymic reaction by an immobilized enzyme immobilized in the fibrous bundle 1. The reaction solution containing the resultant product is overflown from a fibrous bundle discharging outlet 42 at the other end of the J-shaped reaction tank 4. The overflown reaction solution is fed from an overflow receiving part 43 through a pipe 44 to a reaction solution storage tank 10. On the other hand, the fibrous bundle 1 is taken out of the fibrous bundle discharging outlet 42 of the J-shaped reaction tank 4 by driving rollers 2, introduced through liquid removing rollers 5 into a washing means 6 and washed. After removing the liquid by liquid removing rollers 7, the fibrous bundle 1 is then returned to the original driving rollers 2.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention is an enzyme reaction in which an enzyme reaction is continuously performed using 1llll of elementary immobilized endless fibers! ! It E H.

Conventional enzyme reaction devices are those in which a substrate solution is flowed from the top or bottom of a column or tank filled with enzyme-fixed synthetic fibers cut into single-tube strands;
For fixed packing of long fiber bundles, the substrate solution is flowed vertically.

These conventional scheelite devices have the following problems: (11) Due to insufficient contact between the enzyme-immobilized fiber and the substrate solution, the substrate solution feed rate must be reduced in order to obtain the desired enzyme reaction yield;
(!) Since the enzymatic reaction is carried out with the fibers filled, the contact between the fibers and the substrate solution becomes uneven, and as a result, the enzyme, especially in the substrate inlet, tends to be partially inactivated.
Foreign matter mixed into the substrate solution contaminates the fibers and deactivates the enzyme.

To prevent this, it is necessary to completely stop one reaction and wash it with a washing solution, or to refill it with new enzyme-fixed fibers. It has drawbacks such as:

An object of the present invention is to provide an enzyme reaction device that continuously performs enzyme reactions. Another object of the present invention is that since the #element-immobilized fibers are uniformly packed into the anti-broad paddle, the contact between the enzyme and the substrate solution is uniform and sufficient, resulting in a high concentration of #1. Q. Jin,
The object of the present invention is to provide an enzyme reaction device that can obtain elementary reaction yields. Still another object of the present invention is to constantly wash the enzyme-immobilized fiber bundle and constantly use a fresh substrate solution. It is about providing.

The present invention will be explained in detail below based on embodiments and with reference to drawing I/1.

The invention! 1t11, as shown in Figure 111, connects the endless fiber bundle 1 with the enzyme immobilized and
II. A drive means for driving, for example a roller 2.

Substrate solution contact tank 3 in which the substrate solution is brought into contact with this fiber bundle l
and a single-shaped warping tank 4. This device further includes:
A first dewatering means for dewatering the fiber bundle from the single-shaped anti-wide tank 4, for example, a dewatering roller b; a cleaning means 6 for cleaning the deliquified fiber bundle; A second liquid removing means, such as a liquid removing roller 7, is provided.

As shown in FIGS. 2(m) and 2(b), the substrate solution contact tank S has a fiber bundle inlet 31 on the upper side and a fiber bundle outlet 32 on the lower side. A substrate solution supply port, for example, a showering pipe 34 is provided on the side wall.

Bottom surface m5st of this contact tank 3, fiber bundle outlet 8 at the center of the bottom surface
2, and the outlet protrudes toward the fiber bundle supply port 41 of the single-shaped reaction tank 4 located below. The showering pipe 84 is disposed above the inner wall of the contact tank 8 and supplies the substrate from the substrate solution supply tank 8 into the contact tank 3 via the pump 9. This contact tank 3
are further parallel to each other (b) moving axis s6 and moving axis I
The six rotation axes B6 having I7 * rotate the poultry tank 3 by a predetermined angle f around the 1b1 movement axis. The moving shaft 87 reciprocates the contact tank 8 by a predetermined distance in the direction of the moving shaft. Due to these rotating shafts and moving shafts, the fiber bundle outlet 32 of the contact tank 8 periodically moves back and forth and left and right above the fiber bundle supply port 41 of the single-shaped reaction tank 4 . Therefore, the fiber bundle 1 entering the contact tank 3 from the fiber bundle entry port 31 comes into contact with the substrate solution spouted from the showering pipe 34 and is impregnated with the substrate solution, while the fiber bundle exit 32 due to its own weight! 1-shaped reaction tank 4 below
Head to.

Since the contact tank 3 periodically moves back and forth and left and right, the fiber bundle impregnated with the substrate solution is swung in a predetermined direction from the fiber bundle outlet 31+ and is orderly filled into the reaction tank 4 from the J-shaped reaction tank fiber bundle supply port 41. The excess amount of substrate solution supplied through the showering pipe 84 is supplied from the fiber bundle outlet Bz to the single-shaped reaction 44 through the fiber bundle supply port 41.

The substrate solution here undergoes an enzymatic reaction by the enzyme immobilized on the fiber bundle. The anti-6 liquid containing the products produced by the enzyme reaction is fed to the fiber bundle outlet 4 at the other end of the single-shaped reaction tank 4.
Overflow from 2. The overflowing liquid 6 is supplied from the overflow receiving part 43 to the reaction liquid storage tank 10 through the pipe 44. The fiber bundle is pulled out by the drive roller 2 from the fiber bundle discharge port 42 of the single-shaped non-wide tank 4 and enters the cleaning means 6 via the dewatering roller 6. The cleaning means 6 is configured to clean the fiber bundle by spraying a predetermined buffer onto the fiber bundle by known means or by causing the fiber bundle to pass through a buffer storage tank. The washed fiber bundle is further dehydrated by a dewatering roller 7 and returned to the original state roller. This liquid removal roller can also serve as the driving roller 2.

The contact time of the fiber bundle 1 filled in the single-shaped reaction tank 4 with the tank liquid is determined by its own weight, the substrate solution supply tank 1, the difference in water head between the fiber bundle supply port 41, and the fiber bundle discharge port 42, etc. arbitrarily determined based on The enzyme-immobilized fiber used in the present invention is made by, for example, immobilizing glucose isomerase on saponified ethylene-vinyl acetate copolymer fiber by a consonant bonding method.

Since the device of the present invention has such a configuration and function, it can achieve excellent effects corresponding to the above-mentioned objective.

[Brief explanation of the drawing]

Figure 1 is a schematic diagram showing an embodiment of the device of the present invention.
FIG. 2 is a perspective view showing an embodiment of the substrate solution contact tank 8;
b) is a plan view thereof. 1-# element immobilized fiber bundle, 2--driving roller, S-substrate solution contact tank, 4-J-shaped cull tank, 5.7-removal roller, 6-cleaning means, 8--substrate solution supply tank . 10-Liquid storage tank, 31-Fiber east invasion 0.31! - Textile bundle output, AS-TM moving axis, 37- Moving axis. Above Figure 7r1 (b)

Claims (1)

[Claims] 1(1) An endless fiber bundle in which flnA'E is immobilized. (a drive means for circulating and pivoting the fiber bundle; a substrate solution contact tank that contacts the fiber bundle with a substrate solution flowing in from the substrate solution supply port to impregnate the fiber bundle with the substrate solution. 141 A fiber bundle supply port and substrate solution inlet at one end and a fiber bundle at the other end. The fiber bundle impregnated with the substrate solution from the fiber bundle outlet of the substrate bath liquid contact tank is filled in an orderly manner from the fiber bundle supply port, and the substrate solution from the substrate solution contact tank is supplied to the substrate solution inlet. A first dewatering means disposed near the fiber bundle outlet of the J3-shaped reaction tank. Ill The dewatering means washes the deliquified fiber bundle A cleaning means. (1) A second dewatering means for dewatering the fiber bundle to be cleaned. (A substrate solution supply means for supplying the substrate solution to the 8+# substrate solution contact tank. +j+ From the single-shaped reaction tank. An enzyme reaction apparatus comprising: a reaction solution storage tank for storing outflowing wax; and a reaction liquid storage tank. 21ε The apparatus according to claim 1, wherein the driving means is a roller. The fiber bundle outlet is provided with a parallel rotation axis and a movement axis, and the rotation axis rotates around the axis, and the movement axis reciprocates in the axial direction. The apparatus according to claim 1, which is configured to periodically move back and forth and left and right above the fiber bundle supply port. The apparatus according to claim 1, wherein the outlet is configured to protrude toward the fiber bundle supply port of the J-shaped reaction tank described below.5.The substrate solution supply port of the substrate solution contact tank is porous. The device according to claim 3, wherein the first liquid expelling means is a roller, and the first liquid expelling means is a roller. The apparatus according to claim 1, wherein the liquid removing means is a roller. tg: The apparatus according to claim 1, wherein the liquid removing means is a roller. Apparatus according to scope 1.