JPS5876754A - Data processor for electrophoresis analyzer - Google Patents

Abstract

PURPOSE:To enable an accurate detection of representative signal values for individual ion component zones by selecting the largest of means in an electrophoresis analysis to be recognized as representative signal value for the ion component zone with this as the front end thereof. CONSTITUTION:A data processor 3 is equipped with a smoothing section 5 for calculating a mean per smoothing time gamma with respect to a signal P from an electrophoresis analyzer 2, a boundary detecting section 6 for detecting a boundary section between ion component zones of different types and a selecting section 8 for selecting the largest of means contained in signals P existing until the recognition time T equivalent to multiplication of the time gamma by a specified constant passes after the detection of a desired boundary section. Then, the selected means are recognized as representative signal values for ion component zones with the boundary section as the front end thereof. This ensures a constant representative signal value thereby enabling a highly accurate qualitative analysis regardless of variation in the amount of sample when a drift occurs.

Description

DETAILED DESCRIPTION OF THE INVENTION This issue 1 relates to an electric kneading σ analyzer (a data processing device), and more specifically, it detects individual ions tj2. Relating to a device that can accurately detect the representative (Et No. 1 side) of the minute zone.

``The detector of the ton air analyzer includes a conductivity detector,
heat detector. There are various types of potential gradient IL detectors, and this method can be applied to any of them, but since potential gradient detectors are also popular, we will use them as an example below.

To identify the sample based on the output value of the potential gradient detector, P IJlm (Potential Unit
It is common to use the ``value'' method, but according to this method, each ion lly, minute zone's weighted gradient is
li L, <It is important to seek.

However, the output signal of the military slope detector is irregular I411.
Since the signal includes a noise level of 1 minute and may still exceed 1+iUI, it is necessary to perform some signal processing in order to obtain the positive gradient value 1' higher.

Therefore, by determining the smoothing time τ using a conventional data processing device, calculating the average value p of the signal during that predetermined time τ, and considering the average value p as the (S value) during that period, the smoothing time τ is determined. , +411 noise components are canceled by A'.The length of h1τ at a given time is always 4
No. 1 If the change is steep, it will be long, but if it is gradual, it will be determined to be long.8 Also, after the front boundary of a certain ion component zone is detected, the rear boundary is determined.

Average the signal values at the points where the red part is detected, and calculate the average 1i
T! f of the signal by considering i as the signal value for its ionic component zone. 1IIJ is being evened out.

However, the change in I due to drift as shown in Figure 1
When [v) occurs, the conventional device has the disadvantage that the representative signal end changes due to the influence of the fluctuation. In other words, even in the same ion component zone, depending on the amount of drift, the difference in the zone iL varies between when it is fried (solid line) and when it is not mixed (broken line 4). -There was a drawback that the 11th shift changed to P, i>21)2.

The present invention has been made in view of the above circumstances, and is intended to provide a data processing device that eliminates the above-mentioned drawbacks by a simple structure that utilizes the configuration of conventional devices.

Below, based on the example shown in the figure, this emission (the moon is l't'
f escape.

m shown in Fig. 2 is an example of the electric method 1υ) analysis system, and is an example of the analysis system for the non-electrical substance 11σ 'A ii'j1
2i 1: A data processing device (i'fi3) such as a microcomputer and a printer block (4) are located at the river mouth at a distance of 1 width.

When the configuration of the data processing unit 113) is expressed in the form of a machine fjH block, it includes a smoothing unit (6), a boundary detection unit [iil, I
f! l'il' 1fl (i7) and bait calculation processing unit (8).

The 1000th floor part (5) is a non-electric OJ unit 1 device i, '+
'[h; position position, Go P from 't21's no'tector is read every Pa I moe △τ (/ko J - 0.1 seconds), nil'i: +i' ? Input-f, r-i 1st work It
/cost the average value r. Exchange 1. Threadir, half r1゛1 conversion (
1ζ1.1'tIl r (-nx/\τ) to output the average I1iti per (-nx/\τ) 3,1111''11da τ value d:, (When the rise of SrP is predicted to be sudden, Q: [
If it is predicted to be small and gradual, do not set it large.
Ru.

j′M! , field detection capital (61 fct, the above half-slide jXj (,
From (5) -・11. '4hJ 1111. +p1
1. Express the first-order differential and second-order ridges of 7F. , instep/uniform i+
(The boundary between zones of different ion components is detected by comparing the i-th differential value and the second-order differential value with the reference value of the ker constant.

The smoothing section (5) and the boundary detection section (6) are also equipped with Jyuto Soji', but the next clock section (7) and multiplication processing section (8) are new. This constitutes the main part of this invention.

The clock section (7) takes in the smoothed time τ from the smoothing section (i), multiplies it by a constant C at a preset point, and outputs the certified time T. In other words, 'I' = CXτ...(1) is expressed as Hokota. As mentioned above, the value d - times is small when the rise of the signal is fast, and kl is large when the rise is gradual, so the certified time T is large when the rise of the calendar number P is sudden, and small when the rise is slow. Become.

Further, after inputting the boundary detection signal (S) of the clock section (7+kl, the boundary section lLi1), an output signal (IJ) is output when Iif+certification time T has elapsed.

Arithmetic processing unit 18) N:, 4. Boundary detection signal (S)
The average value p input during the period from when is input to when the output number (F) is input is compared moment by moment, and the IIJ large (114) is selected. stomach"
In case of iM, it becomes (E).

Furthermore, the selected 114 large seven-piece value is defined as the representative signal value of the ion component zone that is well known at that time, and based on this, identification is performed using the I]II 11ri method. The above theory, I! As can be understood from ``, the data processing device of this month performs smoothing +b7 on the signal P from the detector of the electrical method ful1 analyzer i.
Ion component zone 1 for the smooth part and the different surface using the IL uniform surface P (per interval τ)? 4. Equipped with a boundary detection unit that detects the JWW capital of '+1', after a certain boundary is detected at [k], 1111 and a half 1f', recognition 1t' of a predetermined faulty f-sound of l conversion time τ! Just select the travel section that selects the maximum value of the average 1'111 input from the detector until T reaches J(ji・IJ-), The average value selected in this way is
Representative of the ionic component zone with death world +1ji and keIII end Irr #li and 1/-” 1u!, '+i4
+l fl+<.

Therefore, referring to Figure 3, as shown in January, even if there is a drift or the sample amount (i.e., ion component zone length) becomes 1. However, if the qualification time T is longer than the required time, spike-like noise will be mixed in and the qualification will be impossible.
Since the J-ability increases, the certified time'r increases by a large amount,
It is better to set it to 1. On the other hand, when the rise of 1d No. P is steep, the maximum 1' surface of the borrow appears immediately after the front boundary of the ion component zone, Kaito, is detected, and when the rise is gradual, it appears after a while. is normal. Therefore, in the case of the songwriter, the certified time T is made clear, and in the latter case, (rj
It is desirable that the By the way, in this IJJ, the certified time T is shorter when the ni is sudden and becomes sharper when it is gradual, as mentioned above, so it is very preferable because it satisfies the above conditions.

[Brief explanation of the drawing]

Figure 1 is an isotachopherogram diagram that describes the operation of conventional ≠ 1. The composition theory (separate diagram, Figure 3 shows the device that produces this power)
It is an isotachopherogram diagram showing the production of No. 1 as jJ/j'+river. (1)...Electrophoresis analysis system, (2)...% Fortune Qi Qixian analysis device u'1'', (3)...
・Data processing device, (4)...printer block,
(5)--Smooth part, (6)--Boundary detection 81<
, (7)... Clock 111), (8)... Enjin processing section.

Claims (1)

[Claims] 1. Electrophoretic analysis-! The signal P from the detector located at
5. On the other hand, a smoothing section that uses the lower average value per smoothing time direction τ and a boundary detection section that detects a boundary between zones of different ion components are used. A selection unit that selects a signal having a large average value that is included in the signal input from the detector during a period where a relay time T that is a predetermined multiple of the averaging time τ has passed has passed. This makes it possible to certify the average value selected as the representative daily price of the AEON reform zone whose front end is the C4 area. a data processing device 14i for an electrophoretic IgIJty analyzer a;