JPS5855861A - 新規な螢光標識抗体及びその製造方法 - Google Patents
新規な螢光標識抗体及びその製造方法Info
- Publication number
- JPS5855861A JPS5855861A JP15379681A JP15379681A JPS5855861A JP S5855861 A JPS5855861 A JP S5855861A JP 15379681 A JP15379681 A JP 15379681A JP 15379681 A JP15379681 A JP 15379681A JP S5855861 A JPS5855861 A JP S5855861A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- dacm
- labeled antibody
- mercaptoethanol
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ADEORFBTPGKHRP-UHFFFAOYSA-N 1-[7-(dimethylamino)-4-methyl-2-oxochromen-3-yl]pyrrole-2,5-dione Chemical compound O=C1OC2=CC(N(C)C)=CC=C2C(C)=C1N1C(=O)C=CC1=O ADEORFBTPGKHRP-UHFFFAOYSA-N 0.000 claims abstract description 37
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims abstract description 35
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims description 4
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 claims 2
- XFZYOTCYWNADMQ-UHFFFAOYSA-N 2-amino-1-sulfanylethanol;hydrochloride Chemical compound Cl.NCC(O)S XFZYOTCYWNADMQ-UHFFFAOYSA-N 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 9
- 239000000872 buffer Substances 0.000 abstract description 8
- 238000006243 chemical reaction Methods 0.000 abstract description 7
- 238000005562 fading Methods 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 5
- 230000007935 neutral effect Effects 0.000 abstract description 4
- 238000002523 gelfiltration Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract 2
- WBBPRCNXBQTYLF-UHFFFAOYSA-N 2-methylthioethanol Chemical compound CSCCO WBBPRCNXBQTYLF-UHFFFAOYSA-N 0.000 abstract 1
- 150000001412 amines Chemical class 0.000 abstract 1
- 239000012295 chemical reaction liquid Substances 0.000 abstract 1
- GWDOTWUCQYXAHN-UHFFFAOYSA-N chloroamine;2-mercaptoethanol Chemical compound ClN.OCCS GWDOTWUCQYXAHN-UHFFFAOYSA-N 0.000 abstract 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 12
- 210000002966 serum Anatomy 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 241000283973 Oryctolagus cuniculus Species 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 230000009871 nonspecific binding Effects 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 238000009739 binding Methods 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000000295 emission spectrum Methods 0.000 description 2
- 238000000760 immunoelectrophoresis Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 208000008439 Biliary Liver Cirrhosis Diseases 0.000 description 1
- 208000033222 Biliary cirrhosis primary Diseases 0.000 description 1
- 206010018372 Glomerulonephritis membranous Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010029719 Nonspecific reaction Diseases 0.000 description 1
- 208000012654 Primary biliary cholangitis Diseases 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- -1 amine hydrochloride Chemical class 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000010504 bond cleavage reaction Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- XLJMAIOERFSOGZ-UHFFFAOYSA-M cyanate Chemical compound [O-]C#N XLJMAIOERFSOGZ-UHFFFAOYSA-M 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 210000001914 gastric parietal cell Anatomy 0.000 description 1
- 210000005104 human peripheral blood lymphocyte Anatomy 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 201000008350 membranous glomerulonephritis Diseases 0.000 description 1
- 231100000855 membranous nephropathy Toxicity 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15379681A JPS5855861A (ja) | 1981-09-30 | 1981-09-30 | 新規な螢光標識抗体及びその製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15379681A JPS5855861A (ja) | 1981-09-30 | 1981-09-30 | 新規な螢光標識抗体及びその製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5855861A true JPS5855861A (ja) | 1983-04-02 |
| JPS6149624B2 JPS6149624B2 (enrdf_load_stackoverflow) | 1986-10-30 |
Family
ID=15570311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15379681A Granted JPS5855861A (ja) | 1981-09-30 | 1981-09-30 | 新規な螢光標識抗体及びその製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5855861A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6432170A (en) * | 1987-07-29 | 1989-02-02 | Agency Ind Science Techn | Production of novel fluorescent labeled antibody |
| EP2036953A3 (en) * | 2004-05-21 | 2010-07-14 | Becton, Dickinson & Company | Long wavelength thiol-reactive fluorophores |
| EP3745129A4 (en) * | 2018-03-23 | 2021-03-24 | Konica Minolta, Inc. | MARKED ANTIBODY DISPERSION LIQUID AND KIT FOR SPFS |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016129444A1 (ja) * | 2015-02-12 | 2016-08-18 | コニカミノルタ株式会社 | 抗体結合蛍光体集積ナノ粒子、抗体結合蛍光体集積ナノ粒子の製造方法および免疫染色キット |
| US11105807B2 (en) | 2015-09-28 | 2021-08-31 | Konica Minolta, Inc. | Method for estimating pathological tissue diagnosis result (Gleason score) of prostate cancer |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5113035A (en) * | 1974-07-23 | 1976-02-02 | Mitsubishi Heavy Ind Ltd | Nainenkikanno jidontensochi |
| JPS53124682A (en) * | 1977-04-08 | 1978-10-31 | Asahi Chem Ind Co Ltd | Preparation of complex of enzyme and bioactive substance and its use |
-
1981
- 1981-09-30 JP JP15379681A patent/JPS5855861A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5113035A (en) * | 1974-07-23 | 1976-02-02 | Mitsubishi Heavy Ind Ltd | Nainenkikanno jidontensochi |
| JPS53124682A (en) * | 1977-04-08 | 1978-10-31 | Asahi Chem Ind Co Ltd | Preparation of complex of enzyme and bioactive substance and its use |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6432170A (en) * | 1987-07-29 | 1989-02-02 | Agency Ind Science Techn | Production of novel fluorescent labeled antibody |
| EP2036953A3 (en) * | 2004-05-21 | 2010-07-14 | Becton, Dickinson & Company | Long wavelength thiol-reactive fluorophores |
| EP3745129A4 (en) * | 2018-03-23 | 2021-03-24 | Konica Minolta, Inc. | MARKED ANTIBODY DISPERSION LIQUID AND KIT FOR SPFS |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6149624B2 (enrdf_load_stackoverflow) | 1986-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Becker et al. | Surface IgE on human basophils during histamine release | |
| Lu et al. | Tutorial review. Oriented immobilization of antibodies and its applications in immunoassays and immunosensors | |
| US5523210A (en) | Bispecific antibody determinants | |
| US4470925A (en) | Immunoglobulin half-molecules and process for producing hybrid antibodies | |
| Frankel et al. | Monoclonal antibodies to a human prostate antigen | |
| EP0108805A1 (en) | Monoclonal antibody mixtures and use thereof for enhanced sensitivity imunoassays | |
| US5272257A (en) | Method of preferential labelling of a phycobiliprotein with an amine-reactive dye for use in a multiple color assay and product for such use | |
| AU595159B2 (en) | Bispecific antibody determinants | |
| JPS5855861A (ja) | 新規な螢光標識抗体及びその製造方法 | |
| Montgomery | Molecular aspects of equine antibodies | |
| JPS62106369A (ja) | 免疫測定法 | |
| Allegrini et al. | Site‐Directed Fluorogenic Modification of Bacteriophodopsin by 7‐Chloro‐4‐nitrobenz‐2‐oxa‐1, 3‐diazole | |
| US5783673A (en) | Method of preferential labelling of a phycobiliprotein with an aminereactive dye for use in a multiple color assay and product for such use | |
| Versée et al. | Rat α-foetoprotein. Purification, physicochemical characterization, oestrogen-binding properties and chemical modification of the thiol group | |
| Motsenbocker et al. | Photoactive methylene blue dye derivatives suitable for coupling to protein | |
| IE881290L (en) | Monoclonal antibodies for the selective immunological¹determination of intact procollagen peptide (Type III) and¹procollagen (Type III) in body fluids | |
| Lauritzen et al. | Peptide dot immunoassay and immunoblotting: Electroblotting from aluminum thin‐layer chromatography plates and isoelectric focusing gels to activated nitrocellulose | |
| JP7710028B2 (ja) | 不溶性粒子、標的抗原測定用又は標的抗体測定用キット、標的抗原又は標的抗体の測定方法及び不溶性粒子を製造する方法 | |
| CN110563882A (zh) | 一种靶向心肌肌钙蛋白i的纳米人工抗体的制备方法与用途 | |
| JPH01175945A (ja) | 抗ビリルビンモノクローナル抗体固定担体およびその製造方法 | |
| JPH07122622B2 (ja) | 免疫センサ− | |
| JPS5960260A (ja) | 酵素免疫測定法 | |
| JP3728757B2 (ja) | 蛍光標識抗体の製造方法 | |
| JP4196376B2 (ja) | フルクトシルアミノ酸標識物を用いたＨｂＡ１ｃの免疫的測定方法 | |
| US4942136A (en) | Method for production of fluorescence-labeled Fab' |