JPS5853738A - Measuring device for volume of particulate - Google Patents
Measuring device for volume of particulateInfo
- Publication number
- JPS5853738A JPS5853738A JP56152297A JP15229781A JPS5853738A JP S5853738 A JPS5853738 A JP S5853738A JP 56152297 A JP56152297 A JP 56152297A JP 15229781 A JP15229781 A JP 15229781A JP S5853738 A JPS5853738 A JP S5853738A
- Authority
- JP
- Japan
- Prior art keywords
- amplifier
- pulse
- electrode
- voltage
- pore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011148 porous material Substances 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 9
- IJJWOSAXNHWBPR-HUBLWGQQSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-(6-hydrazinyl-6-oxohexyl)pentanamide Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)NCCCCCC(=O)NN)SC[C@@H]21 IJJWOSAXNHWBPR-HUBLWGQQSA-N 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims 1
- 239000000725 suspension Substances 0.000 abstract 3
- 239000010419 fine particle Substances 0.000 description 11
- 238000005259 measurement Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 2
- 239000008151 electrolyte solution Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000004323 axial length Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012777 electrically insulating material Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000010979 ruby Substances 0.000 description 1
- 229910001750 ruby Inorganic materials 0.000 description 1
- 229910052594 sapphire Inorganic materials 0.000 description 1
- 239000010980 sapphire Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
- G01N15/131—Details
- G01N15/132—Circuits
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は自球や動植物の細胞、等の微粒子を電解液など
の浮遊液に混和させてその体積を測定する測定装置に関
する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a measuring device for measuring the volume of microparticles such as self-sphere, animal and plant cells, etc. mixed in a suspended liquid such as an electrolytic solution.
この種■定装置の動作原理を説明すれば、電解液などの
浮遊液＾に微粒子ｂｅｉｍ和させ、その浮遊液中に微粒
子わが１つ以上同時に通過しない程度に狭隘に形成され
た細孔１を有する検出器本体２を入れ、検出器本体２内
部を陽圧状態となして細孔１から微粒子すを通過させ、
その時微粒子と浮遊液との藺の電気抵抗、詳しくは内部
電極３と外部電極４との閤の電気抵抗が変化するので、
この変化を電気的パルスとして取出して微粒子もの体積
を測定するようになっている。To explain the operating principle of this type of device, fine particles are mixed with a suspended liquid such as an electrolytic solution, and a pore 1 formed so narrow that one or more fine particles cannot pass through at the same time is inserted into the suspended liquid. A detector main body 2 having the above structure is inserted, the inside of the detector main body 2 is brought into a positive pressure state, and the fine particles are passed through the pores 1.
At that time, the electrical resistance between the particles and the suspended liquid, specifically the electrical resistance between the internal electrode 3 and the external electrode 4, changes.
This change is extracted as an electrical pulse to measure the volume of the particles.
係る測定＠瞳は取り扱いは簡単であるが、浮遊液の比抵
抗変化によって粒子測定パルスの波高値が蛮動しやすく
、測定温度の違いが測定誤差を引き起す原因となってい
た。Such measurement@pupil is easy to handle, but the peak value of the particle measurement pulse tends to fluctuate due to changes in the specific resistance of the suspended liquid, and differences in measurement temperature cause measurement errors.
本発明はこの様な従来暎冒の欠点に鑑み、簡単な構成で
広い温度範囲にわたり安定した測定パルスか得られる、
信頼性の高い微粒子体積測定装置を提供せんとするもの
である。In view of these conventional drawbacks, the present invention has a simple configuration that allows stable measurement pulses to be obtained over a wide temperature range.
The present invention aims to provide a highly reliable particle volume measuring device.
以下、本発明実施の一例を図面に基づいて説明すれば、
検出器本体２はガラス等の電気的絶縁材で中空形状に形
成し、内部に内部電極３を段重すると共に、下部には細
孔１を、更に上部には微粒子すと浮遊液ａを細孔から定
圧、定速で吸引する為の定曇装置５を取付けてなる。Hereinafter, an example of implementing the present invention will be described based on the drawings.
The detector main body 2 is formed in a hollow shape using an electrically insulating material such as glass, and internal electrodes 3 are layered inside, and a pore 1 is formed in the lower part, and a suspended liquid a is formed in the upper part to form fine particles. A constant fogging device 5 is attached to draw suction from the hole at a constant pressure and constant speed.
細孔１はルビーやサファイヤ等のチップ６に開穿され、
微粒子すが１つ以上同時に通過しない程度に狭隘に形成
する。The pore 1 is opened by a chip 6 made of ruby, sapphire, etc.
It is formed so narrowly that one or more particles cannot pass through at the same time.
そして、浮遊液内に外部電極４を入れ、微粒子すが細孔
１を通過する時の微粒子すと浮遊液ａとの電気抵抗変化
を電気的パルスとして取出して微粒子すの体積を測定す
るものである。Then, an external electrode 4 is placed in the suspended liquid, and the change in electrical resistance between the fine particles and the suspended liquid A when the fine particles pass through the pores 1 is extracted as an electrical pulse to measure the volume of the fine particles. be.
その為に、内部電極３には、内部電極３から細孔１を通
して外部電極４に直流一定電流を供給する定電流装置７
と、内部電極３に発生するパルスのみを増幅する交流増
幅器８及び、内部電極３に影響を与えることなく内部電
極３に発生する電圧を取出すバッハ−アンプ９を接続す
る。For this purpose, the internal electrode 3 is equipped with a constant current device 7 that supplies a constant DC current from the internal electrode 3 to the external electrode 4 through the pore 1.
An AC amplifier 8 that amplifies only the pulses generated at the internal electrode 3 and a Bach amplifier 9 that extracts the voltage generated at the internal electrode 3 without affecting the internal electrode 3 are connected.
バッハ−アンプ９には内部電極３に発生した直流成分の
みを取出すローパスフィルター１０を接続し、このロー
パスフィルター１０をアナログ割算器１１の分母入力に
接続し、上記交流増幅器８をアナログ割算器１１の分子
入力に接続してなる。A low-pass filter 10 is connected to the Bach amplifier 9 for extracting only the DC component generated in the internal electrode 3. This low-pass filter 10 is connected to the denominator input of an analog divider 11, and the AC amplifier 8 is connected to the analog divider 11. It is connected to 11 molecular inputs.
然して、細孔１間の電気抵抗Ｒは、Ｔ’Ｃにおける浮遊
液ａの比抵抗日と微粒子すの比抵抗ｌとが８（職 とす
れば、次の式で現わせる。Therefore, the electrical resistance R between the pores 1 can be expressed by the following formula, assuming that the specific resistance of the suspended liquid a at T'C and the specific resistance l of the fine particles are 8 (d).
Ｒ−ＦＦ？・ｄ／Ｓ＋Ｐ−ｖ／ＳＪ
式中Ｓは細孔１の断面積、ｄは細孔の軸方向の長さ、■
は微粒子体積を現わす。R-FF?・d/S+P-v/SJ In the formula, S is the cross-sectional area of pore 1, d is the axial length of the pore, ■
represents the particle volume.
今ここで、細、孔１間に一定電流■が流れているとする
と、内部電極３に発生する電圧Ｖは、Ｖ今Ｉ−Ｒ
−Ｉ （Ｆ？−ｄ／Ｓ＋Ｆ７−ｖ／Ｓ’）となる。この
電圧Ｖは細孔１の電気抵抗によって発生する電圧Ｍと、
微粒子すの電気抵抗によって発゛生ずるパルス電圧■と
の合成電圧なので、Ｖ、 −Ｒ−ｄ ・ Ｉ／Ｓ
Ｖ、−Ｐ、−Ｖ −Ｉ／Ｓ”
とすると、Ｖ ＝ Ｖｌ ＋ Ｖｌとなり、結局ｖ１は
直流電圧であり、■はパルス電圧であるご仁になる。Now, if a constant current ■ is flowing between the thin hole 1, the voltage V generated at the internal electrode 3 is VI-R-I (F?-d/S+F7-v/S') becomes. This voltage V is the voltage M generated by the electrical resistance of the pore 1,
Since it is a composite voltage with the pulse voltage ■ generated by the electrical resistance of the fine particles, V, -R-d ・I/S V, -P, -V -I/S'', then V = Vl + Vl, After all, v1 is a DC voltage, and ■ is a pulse voltage.
ここで、内部電極３に発生する電圧■は交流増幅器とバ
ッハ−アンプ９に伝送されてアナログ割゛禅器１１に伝
達されるが、交流増幅器８によりパルス電圧■成分のみ
が増幅されてアナログ割算器１１の分子入力に伝達され
、他方バッハ−アンプ９に伝送された電圧Ｖはローパス
フィルター１０により直流電圧■、酸成分みがアナログ
割算器１１の分母入りに伝達される。即ち、交流増幅器
８の増！１度をＡとす−れば、アナログ割算器１１の分
゛子入力は、
Ａ−Ｖ、＝７Ａ−Ｆ？−Ｖ −１／Ｓ”となり、アナロ
グ割算器１１の分母入力は、ｙ、＝ｐ−ｄ−Ｉ／Ｓ
。Here, the voltage ■ generated at the internal electrode 3 is transmitted to the AC amplifier and the Bach amplifier 9, and then to the analog divider 11, but only the pulse voltage ■ component is amplified by the AC amplifier 8, and the analog divider The voltage V transmitted to the numerator input of the multiplier 11 and the Bach amplifier 9 on the other hand is transmitted to the denominator input of the analog divider 11 with only the DC voltage (2) and the acid component being transmitted by the low-pass filter 10 . In other words, the number of AC amplifiers 8 is increased! If 1 degree is A, then the denominator input of analog divider 11 is A-V, =7A-F? −V −1/S”, and the denominator input of the analog divider 11 is y, =p−d−I/S
.
となるから、アナログ割算器１１の出力Ｙは、Ｙ−Ａ婚
Ｖ、／込
１１．−コニし工」ヱ泊−・１
（Ｐ−ｄ ・Ｉ／５）
−Ａ・ｖ／Ｓ−ｄ
となり、結局パルス電圧は浮遊液ａの比抵抗弓には依存
しないことが解る。Therefore, the output Y of the analog divider 11 is Y-A marriage V, /inclusive 11. -Konishiko'Edomari-・1 (P-d・I/5) −A・v/S-d, and it can be seen that the pulse voltage does not depend on the resistivity arc of the floating liquid a.
尚、電圧パルスを取出した後の処理は特に図示しないが
、従来品と同様である。Note that the processing after taking out the voltage pulse is not particularly shown in the drawings, but is similar to the conventional product.
本発明は斯様に、内部電極と外部電極との闇に直流一定
電流を流す定電流装置と、内部電極に一生するパルスの
みを増幅する交流増幅器と、内部電極に発生する電圧を
取出すバッハ−アンプ及びその直流成分のみをとりだす
Ｏ−バスフィルターと、増幅されたパルスの波高値とロ
ーパスフィルターとの出力の比をとるアナログ割算器と
で構成したので、測定温度の変化による浮遊液の比抵抗
変化が無視でき、よって広い温度範囲にわたって安定し
た測定パルスが得られ、信頼性の高いものとなる。In this way, the present invention includes a constant current device that flows a constant DC current between the internal electrode and the external electrode, an AC amplifier that amplifies only the pulse that is generated in the internal electrode, and a bach amplifier that extracts the voltage generated in the internal electrode. It consists of an amplifier, an O-bass filter that takes out only its DC component, and an analog divider that takes the ratio of the peak value of the amplified pulse to the output of the low-pass filter, so the ratio of suspended liquid due to changes in measurement temperature can be adjusted. Resistance changes are negligible, resulting in stable measurement pulses over a wide temperature range and high reliability.
しかも、その構成が簡単であり、安価に提供でき、その
実用的効果は大なるものがある。Moreover, it has a simple configuration, can be provided at low cost, and has great practical effects.
よって、所期の目的を達成し得る。Therefore, the intended purpose can be achieved.
、４、図面の簡単な説明
′ −
第１図は本発明実施の一例を示す回路図、第２図は細孔
と微粒子との関係を説明する概念図である。, 4. Brief Description of the Drawings' - Fig. 1 is a circuit diagram showing an example of the implementation of the present invention, and Fig. 2 is a conceptual diagram illustrating the relationship between pores and fine particles.
図中、１は細孔、２は検出器本体、３は内部型°極、４
は外部電極、５は定１１＠置、６はチップ、７は定電流
装置、８は交流増幅器、９はバッハ−アンプ、１０はロ
ーパスフィルター、１１はアナログ割算器、ａは浮遊液
、ｂは微粒子、である。In the figure, 1 is the pore, 2 is the detector body, 3 is the internal type pole, and 4
is an external electrode, 5 is a constant 11@ position, 6 is a chip, 7 is a constant current device, 8 is an AC amplifier, 9 is a Bach amplifier, 10 is a low-pass filter, 11 is an analog divider, a is a floating liquid, b is a fine particle.
特許出願人 エルマ光学株式会社Patent applicant: Elma Optical Co., Ltd.
Claims (1)
と浮遊液との電気抵抗変化を電気的パルスとして取出し
て微粒子体積を測定する装置において、検出一本体に組
込んだ内部電極と外部電極との圀に直流一定電流を流す
定電Ｉｌ＠置と、内部電極に発生するパルスのみを増幅
する交流増幅器と、内部電極に発生する電圧を取出すバ
ッハ−アンプ及びその直流成分のみを取、 出すロー
パスフィルターと、増幅されたｌ＜ルスの波高値とロー
パスフィルターとの出力の比をとるアナログ割算器と、
からなる事を特徴とする微粒子の体積測定装置。In a device that measures the volume of particles by extracting the electrical resistance change between the particles and the suspended liquid as an electrical pulse when the particles mixed in the suspended liquid pass through a pore, an internal electrode built into the detection body and an external electrode are used. A constant current Il @ device that flows a constant DC current between the electrodes, an AC amplifier that amplifies only the pulses generated at the internal electrodes, a Bach amplifier that extracts the voltage generated at the internal electrodes, and a Bach amplifier that extracts only the DC component. an analog divider that calculates the ratio of the amplified peak value of l<Rus to the output of the low-pass filter;
A particulate volume measuring device characterized by comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56152297A JPS5853738A (en) | 1981-09-25 | 1981-09-25 | Measuring device for volume of particulate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56152297A JPS5853738A (en) | 1981-09-25 | 1981-09-25 | Measuring device for volume of particulate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5853738A true JPS5853738A (en) | 1983-03-30 |
Family
ID=15537440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56152297A Pending JPS5853738A (en) | 1981-09-25 | 1981-09-25 | Measuring device for volume of particulate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5853738A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4827143A (en) * | 1986-03-26 | 1989-05-02 | Hitachi, Ltd. | Monitor for particles of various materials |
| JPH01112448U (en) * | 1988-01-21 | 1989-07-28 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5046179A (en) * | 1973-08-13 | 1975-04-24 | ||
| JPS5068173A (en) * | 1973-10-16 | 1975-06-07 | ||
| JPS513279A (en) * | 1974-06-26 | 1976-01-12 | Toa Electric Co Ltd | Kendakuekichuno ryushikeisokusochi |
| JPS53133487A (en) * | 1977-04-27 | 1978-11-21 | Nippon Kouden Kougiyou Kk | Particle detector |
-
1981
- 1981-09-25 JP JP56152297A patent/JPS5853738A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5046179A (en) * | 1973-08-13 | 1975-04-24 | ||
| JPS5068173A (en) * | 1973-10-16 | 1975-06-07 | ||
| JPS513279A (en) * | 1974-06-26 | 1976-01-12 | Toa Electric Co Ltd | Kendakuekichuno ryushikeisokusochi |
| JPS53133487A (en) * | 1977-04-27 | 1978-11-21 | Nippon Kouden Kougiyou Kk | Particle detector |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4827143A (en) * | 1986-03-26 | 1989-05-02 | Hitachi, Ltd. | Monitor for particles of various materials |
| JPH01112448U (en) * | 1988-01-21 | 1989-07-28 |
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