JPS5840100A - Measurement of uric acid and device therefor - Google Patents
Measurement of uric acid and device thereforInfo
- Publication number
- JPS5840100A JPS5840100A JP13909681A JP13909681A JPS5840100A JP S5840100 A JPS5840100 A JP S5840100A JP 13909681 A JP13909681 A JP 13909681A JP 13909681 A JP13909681 A JP 13909681A JP S5840100 A JPS5840100 A JP S5840100A
- Authority
- JP
- Japan
- Prior art keywords
- chamber
- uric acid
- carbon dioxide
- absorption liquid
- inlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、血液等の被検体に含ま)する尿酸の濃度を吸
収液の導電率変化線から間接的に測定p b尿酸の測定
方法および測定装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method and apparatus for measuring pb uric acid, which indirectly measures the concentration of uric acid (contained in a sample such as blood) from a conductivity change line of an absorption liquid.
近年、生医学的測定法の発展により、体温、胃腸内の圧
力、血圧、呼吸の速度、および生物学的ポテンシャルの
ようh生理学的変数を連続的に遠隔測定した9、生体内
におけるpO2,pCO2、血液のpHおよび電解質、
並びに胃のpHを連続的に測定うになった。このよう寿
牛医学的測定法の−っど1−て、被検体中の尿酸にウリ
カーゼ酵素を作用さ1rて一1式(1)の酵1反応を生
じさぜ、尿酸による紫外Iil吸収駐の変化から被検体
中の尿酸濃度を測定すZ)、いわゆるウリカーゼ紫列部
吸収法がある。In recent years, advances in biomedical measurements have enabled continuous remote measurement of physiological variables such as body temperature, gastrointestinal pressure, blood pressure, rate of respiration, and biological potential9, pO2, pCO2 in vivo. , blood pH and electrolytes,
In addition, the pH of the stomach was measured continuously. In this way, in the first part of the Jugyu medical measurement method, uricase enzyme is applied to uric acid in the sample to cause the fermentation reaction of formula (1), and the ultraviolet absorption of uric acid by uric acid is carried out. There is a so-called uricase purple absorption method that measures the concentration of uric acid in a subject from changes in Z).
ノイ’Jfjt+ 2u2o+02 −、 :1
−ソー計−−−+co242■2o2−一−(1)然し
なから、上記ウリカーゼ紫外部吸収法等の吸光光度法に
おいては、被検体中に直情タンパク等が含li1でいる
場合、該タンパクを除去する必便があり血液が被検体の
ときの全+ru測定が不可能であるという欠点があった
。1だ、発色時間が長くかかるため、被検体中の尿酸を
迅速に測定することができないという欠点もあった。Neu'Jfjt+ 2u2o+02 -, :1
-Saw meter---+co242■2o2-1-(1) However, in the spectrophotometric method such as the above-mentioned uricase ultraviolet absorption method, if the subject contains a direct protein, etc., the protein is detected. There is a drawback that it is impossible to measure total +ru when blood is the subject because stool must be removed. 1. It also had the disadvantage that it took a long time to develop the color, making it impossible to quickly measure uric acid in the sample.
本発明は、かかる欠点に鑑みてなされたものであり、そ
−の目的は、除タンパク等の前処理操作を閥することな
く、全面等の被検体中に含まれる尿酸をも迅速かつ正確
に測定できる尿酸の測定方法オヨび41+11 ’ij
? dl、、 IE?を1h1イj(すルコトニアル。The present invention was made in view of these drawbacks, and its purpose is to quickly and accurately remove uric acid contained in a specimen such as a whole surface, without compromising pretreatment operations such as protein removal. How to measure uric acid 41+11'ij
? dl,, IE? 1h1ij (surcotonial.
以ド、本発明i+rついて図を用いて詳細に説明する。Hereinafter, the present invention i+r will be explained in detail using the drawings.
第1図は、本発明の実施例を示す構成説明図であり、図
中、1i’:lフローセルであ−)て、イオンを通さず
ガスを通す例えばr’TFEやシリコンゴJ、薄膜でな
る隔膜2を介して第1室3と第2室4が隣接している。FIG. 1 is a configuration explanatory diagram showing an embodiment of the present invention, and in the figure, a 1i':l flow cell is made of a thin film such as r'TFE, silicone rubber, etc., which does not allow ions to pass through but allows gas to pass through. A first chamber 3 and a second chamber 4 are adjacent to each other with a diaphragm 2 in between.
1′i&、第1宰3にt、l導入]]5ど導出口51が
設けられ、m2室4にl]導入116ど導出[16Iが
設けられている。更に、2]J:I例えばホウ酸緩衝液
、NaCe1およびE’l’1M安岩香酸等をハむ溶a
聾からなるキャリアが導入される導入口、2:(r、J
該キャリア等の排出口であって、該導入1]21から第
2家4の導入l6に至る流路の途中にけ被検体を注入す
るサンプルインジェクタ27およびガラスピーズ若しく
はナイロンチー−ブ等の坦体1cウリカーゼ固
酵素が固定化されてなる一足化酵累22が設けC,れて
いる。更に1だ、固定化酵素22から第2宰4の導入口
6に至る流路の途中には、例えばIIceや)iNoの
0.IN溶液等でなる酸性試薬を添加するだめの試薬添
加口28が設けられている。一方、24は吸収液導入口
であって、容器25内の1及収液26(例えば0.00
2HのKOII等でなるアルカリ性溶液)を吸引できる
ように設置されている。寸だ、10は吸収液1ノ)出口
であって、第1室3の流出口5・から吸収液刊出ロ1.
0117:至る流路の途中には、例えば電源8と検流□
計9が成続された電極7,7Iが、例えば内径10ゾI
11の配、管に装着されて導出r15′付近に設けられ
ており、導出口5Iから導出される吸収液の導電率を検
出するようになっている。1'i&, the first room 3 is provided with a t, l introduction 116 and an outlet 51, and the m2 chamber 4 is provided with an l] introduction 116 and an egress 16I. Furthermore, 2] J:I, for example, a solution containing borate buffer, NaCe1 and E'l' 1M andenite aromatic acid, etc.
Inlet where carriers consisting of deaf people are introduced, 2: (r, J
A sample injector 27 and a support such as glass beads or nylon tubes are provided at the outlet of the carrier, which injects the specimen into the flow path from the introduction 1] 21 to the introduction 16 of the second housing 4. A single fermenter 22 in which uricase solid enzyme is immobilized is provided. In addition, in the middle of the flow path from the immobilized enzyme 22 to the inlet 6 of the second tube 4, for example, IIce or iNo. A reagent addition port 28 is provided for adding an acidic reagent such as an IN solution. On the other hand, 24 is an absorption liquid introduction port, and 1 and the collected liquid 26 in the container 25 (for example, 0.00
It is installed so that it can aspirate an alkaline solution (such as 2H KOII). 10 is an outlet of the absorbent liquid 1, and the absorbent liquid is output from the outlet 5 of the first chamber 3.
0117: In the middle of the flow path, for example, there is a power supply 8 and a galvanometer □
For example, the electrodes 7, 7I with which a total of 9 are connected have an inner diameter of 10 zoI.
11, and is provided near the outlet r15' to detect the electrical conductivity of the absorption liquid drawn out from the outlet 5I.
−1−記構成からなる本発明の実施例において、導入1
]21から導入されたキャリアは、固定化酵素22、導
入「16、第2室4、および導出口6Iを経て流れ、排
出口23から排出される。また、導入口24から吸引さ
れた吸収液2Gは、導入口5、第1室3、および導出口
5響を紅で流れ、排出口10から排出される。-1- In the embodiment of the present invention consisting of the configuration described above, introduction 1
] 21 flows through the immobilized enzyme 22, the introduction port 16, the second chamber 4, and the outlet port 6I, and is discharged from the outlet port 23. Also, the carrier introduced from the inlet port 24 2G flows red through the inlet 5, the first chamber 3, and the outlet 5, and is discharged from the outlet 10.
ととるで、サンプルインジェクタ27から被検体がギヤ
リア中へ注入されると、該被検体はキャリアに運ばれて
固定化酵素22に至り、前記第(1)式のような酵素反
応を受けてC02やlI202を生ずる。このとき、ギ
ヤリア中のホウ酸緩衝液はph+が845であるため、
上記COから生成される炭酸は重炭酸イ〕ンllCO3
−となっている。しかして、試薬添加口28から酸性試
薬がギヤリア中へ添加され、キ、Yリア等でなる混合溶
液のplfが1以下になると、上記重炭酸イオノ1■C
O3−の大部分が002刀スとなる。Iこのようにして
、被検体中に含1れている尿酸はCO2ガスへ変換され
、ギヤリアによ一部で導入1’16から70−セル1内
の882室4へ運げJする。該第2室4において、ギヤ
1にf中の002ガス0.11分圧該
に比例して隔膜2を透過17第1掌6に至1】、第1室
6において−F式(2)のように吸収液と反応する。1
該反応により吸収率−の導11f1率が変化し、該導〒
IY、率co→−2Iぐ011−−ニーーーーーー六ジ
、に2C03−II+20−−−−−−−−−−、−(
2)変化が」二記電椿7.7+によって検出いノ:、る
。このようにし°C¥M、極7,71によって検出され
た導電、率変化量から、所定の信号処理等(y1示せず
)を経て間接的に被検体中の尿酸が定を−される。Therefore, when the specimen is injected into the gearia from the sample injector 27, the specimen is carried by the carrier and reaches the immobilized enzyme 22, where it undergoes an enzymatic reaction as shown in equation (1) above and becomes C02. and lI202. At this time, since the boric acid buffer in the gearia has a pH+ of 845,
The carbonic acid produced from the above CO is bicarbonate ion llCO3
-. When the acidic reagent is added into the gearia from the reagent addition port 28 and the plf of the mixed solution consisting of Ki, Yria, etc. becomes 1 or less, the bicarbonate ion 1■C
Most of O3- will be 002 swords. In this way, the uric acid contained in the subject is converted into CO2 gas, which is transported by the gear from the introduction 1'16 to the 882 chamber 4 in the 70-cell 1. In the second chamber 4, the partial pressure of the 002 gas in the gear 1 is proportional to 0.11, and the diaphragm 2 passes through the diaphragm 17 to reach the first palm 6. In the first chamber 6, -F formula (2) Reacts with the absorbing liquid like this. 1
Due to this reaction, the absorption rate - lead 11f1 rate changes, and the lead 〒
IY, rate co→-2Igu011--neeee-6ji, ni2C03-II+20------------,-(
2) The change is detected by ``Niki Dentsubaki 7.7+''. In this way, the uric acid in the subject is determined indirectly from the conductivity and rate change detected by the electrodes 7 and 71 through predetermined signal processing (y1 not shown).
また、第2図は、本発明実施例に」?けるノロ−セルの
分解斜視図、第3図tJ第2図の組立余111−図であ
る。第2図および第5図におい”C1i、1.1.7i
Jブロツクであって、夫々導入l’、、l 1.1.a
、 1.7nと導出1111b、 17bが設けられて
おり、1.2.15 rJ、ガスケツlであっ−C夫々
第1室および第2室を形成する空洞部1.3.1.6が
設けられている0また、14はイオンを通さずガスを通
す隔膜である。−1二記構成からなるフ「1−ヒルにお
いて、被検体等を含むキャリアは導入II 1.7aか
ら導入され空洞部16を経て導出[1171)から導出
さね、吸収液は導入口11aから導入され空1lBI音
1s13を経て導出口111)から導出される。Also, FIG. 2 shows an embodiment of the present invention. FIG. 3 is an exploded perspective view of the norocell shown in FIG. 3; FIG. In Figures 2 and 5 "C1i, 1.1.7i"
J block, each introduced l',,l 1.1. a
, 1.7n and leads 1111b, 17b are provided, and a cavity 1.3.1.6 forming the first and second chambers, respectively, is provided with 1.2.15 rJ and a gasket l. Also, 14 is a diaphragm that does not allow ions to pass through but allows gas to pass through. -1 In the 1-Hill consisting of two configurations, the carrier containing the analyte etc. is introduced from the introduction II 1.7a, passes through the cavity 16 and is led out from the outlet [1171], and the absorption liquid is introduced from the introduction port 11a. The sound is introduced through the air 1lBI sound 1s13 and is led out from the outlet 111).
1だ、空洞部16を流れるキャリアに含1れているCO
ガスt、l、隔膜14を透過して空洞部13に達し、空
洞部1:Iを流れる吸収液と反応する。1, CO contained in the carrier flowing through the cavity 16
The gases t and l pass through the diaphragm 14, reach the cavity 13, and react with the absorption liquid flowing through the cavity 1:I.
以1−1詳しく説明したような本発明の実施例にX、;
1.げ、除タン・ζり等の前処理操作を要するとと′l
く、被検体中に含1れる尿酸を迅速かつ正確にi++1
0]!できるという利点を有する。X in the embodiment of the present invention as described in detail in 1-1 below;
1. Requires pre-treatment operations such as drying, sludge removal, and rinsing.
It quickly and accurately detects uric acid contained in the sample.
0]! It has the advantage of being able to
IJiに、第4図は、本発明の他の実施例を示す構成1
191明図であり、図中、コシ9は吸収液導入口、30
け田/プルインジェクタ27から注入された被検体を太
りのギヤリア中へ流路切換によって供給するサンプル切
換パルプである。寸だ、第4図において、第1図と同−
数字の記号(例えば、fi’a、 5’bは51と同一
数字の記号である)れ1回−意味をもたけて使用し、こ
こでの重複説明目省略する。上記構成からkる本発明他
の実施例において、導入「121から導入されたキャリ
アの一部は、導入1−1(紬、フローセル1aの第2室
4as および導出1”’ 6’a %を経て排出口2
3aから排出されると共に、残り+J固定化酵素22、
導入口6b、フローセル11)の第2室4b、」・・よ
び導出D6Ibを経て]非出n 23iから4井出され
る。In IJi, FIG. 4 shows a configuration 1 showing another embodiment of the present invention.
This is a clear diagram of 191, and in the diagram, 9 indicates the absorption liquid inlet, and 30
This is a sample switching pulp that supplies the analyte injected from the Keda/Pull injector 27 into the thick gearia by switching the flow path. In figure 4, it is the same as in figure 1.
Numerical symbols (for example, fi'a, 5'b are the same numerical symbols as 51) are used once to convey their meaning, and redundant explanations will be omitted here. In another embodiment of the present invention based on the above configuration, a part of the carrier introduced from the introduction 121 is transferred to the second chamber 4as of the flow cell 1a and the second chamber 4as of the flow cell 1a and the outlet 1''6'a%. via outlet 2
3a, and the remaining +J-immobilized enzyme 22,
4 through the inlet 6b, the second chamber 4b of the flow cell 11), and the lead-out D6Ib].
また、容器25内の吸収液26はその−81(が、吸収
液導入口24から導入され、導入口5b、)■]−1!
ル11〕の第1室31)、および流出ITI 5’l)
を経て流、れ、排出口10bから排出されるとともに、
他の一部0吸収液導入口29から導入され、導入17j
5+1、フローセル1aの第1室3a、および流出[
二15マaを糸Yて健、ハ、J)+出口10aから排出
される。ところで、サンプルインジェクタ27から注入
された所定量の被検体がフローセル1aに至る第1のギ
ヤリアffl+、路にサンプル切換パルプ30を経由し
て供給されるど、該被検体はキャリアによって運げノ1
−るが、試薬添加II 2B11から酸性試薬が添加さ
れると、上記被検体中に炭酸イオノi・重炭酸イオンが
含1れている場合、とれらのイオンがCO2ガスへ変換
される。その後、i” CO2ガスや被検体はギヤリア
によってフローセル−a内の第2宰4aへ運げれ、該C
02ガスだけが分用に比例1.て隔IIφ2aを透過し
第1室3aに至って、前記第(2)式のように吸収液と
反応する。該反応により第1I<3+を内の吸収液の導
電1率が変化し、該導電率変化が電極7n、、 7’a
によって検出される。また、フローセル1aの第2宰4
a内の被検体1、上述のようにして炭酸イオンや重炭酸
イオンが除去されてのち、再びキャリアに運ばれ導出口
6’aを経て排出ITI 23aから排出される。一方
、ザンプルイ7ノ、クタ27からYl:入された所定量
の被検体が固定化酵素22に至る第2のキャリア流路に
サンプル切換パルプ30を経由して供給されると、該被
検体1′:[キャリアによって運ばれて固定化酵素22
に至り、前記第(1)式のような酵素反応を受けてCO
2やlI202を生ずる。このど籾、キャリア中のホウ
酸緩衝液しl’、 plfが8.5で、ちるため、−1
−記CO2から生成される炭酸0重炭酸イオンlIc0
−となっている。また、試薬添加口281〕から酸性試
薬がキャリ゛ア中へ添加され、キャリア等でなる混合溶
液のpIIが1以下になると、上記重炭酸イオン1■C
O3−の太部6)がC02ガスと々る。しかして、被検
体中に含1れている尿酸はC02ガスへ変換され、ギ、
v IJアによっで導入口6bからフローセル1b内の
第2室4bへ運ばノする。Moreover, the absorption liquid 26 in the container 25 is introduced from the absorption liquid introduction port 24, and the absorption liquid 26 is introduced from the absorption liquid introduction port 24, and the absorption liquid 26 is introduced from the introduction port 5b,)] -1!
11), and the outflow ITI 5'l)
and is discharged from the discharge port 10b,
The other part 0 is introduced from the absorption liquid introduction port 29, and the introduction 17j
5+1, the first chamber 3a of the flow cell 1a, and the outflow [
215 ma is threaded through the thread Y, ha, J) + is discharged from the outlet 10a. By the way, when a predetermined amount of the analyte injected from the sample injector 27 is supplied to the flow cell 1a through the sample switching pulp 30, the analyte cannot be carried by the carrier.
However, when the acidic reagent is added from Reagent Addition II 2B11, if carbonate ion and bicarbonate ions are included in the above-mentioned sample, these ions are converted to CO2 gas. After that, the CO2 gas and the specimen are transported to the second chamber 4a in the flow cell-a by the gear rear, and the
Only 02 gas is proportional to the distribution 1. The liquid then passes through the partition IIφ2a and reaches the first chamber 3a, where it reacts with the absorption liquid as shown in equation (2) above. Due to this reaction, the conductivity of the absorption liquid in the first I<3+ changes, and this change in conductivity causes the electrodes 7n, 7'a
detected by. In addition, the second chamber 4 of the flow cell 1a
After carbonate ions and bicarbonate ions are removed from the specimen 1 in a as described above, it is transported again to the carrier and discharged from the discharge ITI 23a via the outlet 6'a. On the other hand, when a predetermined amount of the analyte introduced from Zampurui 7 and Kuta 27 is supplied to the second carrier channel leading to the immobilized enzyme 22 via the sample switching pulp 30, the analyte 1 ′: [immobilized enzyme 22 carried by carrier
The CO
2 and lI202. In this case, the boric acid buffer in the carrier l', plf is 8.5, so it is -1
-carbonate 0 bicarbonate ion lIc0 produced from CO2
-. Further, when an acidic reagent is added into the carrier from the reagent addition port 281 and the pII of the mixed solution consisting of the carrier etc. becomes 1 or less, the bicarbonate ion 1C
The thick part 6) of O3- reaches the C02 gas. Therefore, the uric acid contained in the sample is converted to CO2 gas,
v The IJ is carried from the inlet 6b to the second chamber 4b in the flow cell 1b.
該第2室4bにおいて、ギヤリア中のC02ガス目分圧
に比例して隔膜2を透過し、第1室31) K至っ′C
前記第(2)式のように吸収液と反応する。該反応によ
り第1室3b内の吸収液の導電1率が変化17、該導電
率変化が電極7b、 71bによっで検出される。従っ
て、該電極7b、 7’bによって検出された吸収液の
導電率変化用から」二記電極7a、 7’aによって検
出された吸収液の導電事変化石を差し引くように所定の
演算処理(図示せず)が施さハると、キャリア中や被検
体中に炭酸イオンや重炭酸・イオンが含1れている場合
でも、これらのイオンと1」無関係に被検体中の尿酸が
測定される。In the second chamber 4b, C02 gas permeates through the diaphragm 2 in proportion to the partial pressure of the C02 gas in the gear rear, and the first chamber 31) reaches K'C.
It reacts with the absorption liquid as shown in equation (2) above. Due to this reaction, the conductivity of the absorption liquid in the first chamber 3b changes 17, and this change in conductivity is detected by the electrodes 7b and 71b. Therefore, a predetermined calculation process (Fig. (not shown) is applied, even if carbonate ions or bicarbonate ions are contained in the carrier or the sample, uric acid in the sample will be measured regardless of these ions.
以上、詳しく説明したような本発明他の実施例によれば
、被検体中に炭酸イオンや11を炭酸イオンが含“まれ
でいる場合でも、寸だキャリア中に炭酸・イオンや重炭
酸イオンが含捷れる場合でも、これC)のイ」ンに妨害
されるととなく、被検体中の尿酸を′11〜速か1)正
確に測定できるという利点を有すZl。According to other embodiments of the present invention as described in detail above, even if carbonate ions or 11 carbonate ions are rarely contained in the specimen, carbonate ions or bicarbonate ions are present in the carrier. Zl has the advantage of being able to accurately measure uric acid in a test sample quickly and without being interfered with by C), even if it is dissolved.
第1図に11、本発明の実施例を示す構成説明図、第2
図および第6図は、本発明実施例のフローセルの分解斜
田図および組立余1視図、第4図は本発明他の実施例を
示す構成説明図である。
1.1a、1+J・・・フローセル、2.2a、 2+
)−隔膜、3.3a。
3L+−第1字、4.4a、仙・・・第2室、5.5a
、 5b、 6.6a。
Gb、 21.、24.29・”導入[−1,5’、
5’a、 5’b、 6’、 6’a、、 6’b・・
・導出[1、?、 7n、 7b、 7°、 7’a、
7’b −1[極、10.10a。
10b、 23 ・・・排出口、25・・・答器、2
6・・・吸収液、27・・・−リーンプルインジェクタ
、28.28a、 28b・・・試薬添加口、30・・
・リンプル切換パルプ。
石3 呵
/ltb11, a configuration explanatory diagram showing an embodiment of the present invention, and 2.
6 and 6 are an exploded slanted view and a partially assembled view of a flow cell according to an embodiment of the present invention, and FIG. 4 is a configuration explanatory diagram showing another embodiment of the present invention. 1.1a, 1+J...flow cell, 2.2a, 2+
)-diaphragm, 3.3a. 3L+-1st character, 4.4a, Sen...2nd house, 5.5a
, 5b, 6.6a. Gb, 21. , 24.29・”Introduction [-1,5',
5'a, 5'b, 6', 6'a,, 6'b...
・Derivation [1,? , 7n, 7b, 7°, 7'a,
7'b -1 [Polar, 10.10a. 10b, 23...Discharge port, 25...Response, 2
6...Absorption liquid, 27...-Lean pull injector, 28.28a, 28b...Reagent addition port, 30...
・Rimple switching pulp. Stone 3 呵/ltb
Claims (3)
せて酵素反応を生じさせる手段と、酸を添加して前記酵
素反応で生じた炭酸イオン若しくは重炭酸イオンを炭酸
ガスに変換さ亡る手段と、イオンを通さずガスを通す隔
膜へ前記炭酸ガスを透過させることにより該炭酸ガスを
塩基性吸収液に吸収させる手段と、該塩基性吸収液の導
電率変化量を検出する手段とを講じて、前記吸収液の導
電率変化lから間接的に被検体中の尿酸を測定すること
を特徴とする尿酸の測定方法。(1) A means for causing an enzymatic reaction by causing uricase to act on uric acid contained in the specimen, and a means for adding an acid to convert carbonate ions or bicarbonate ions generated in the enzymatic reaction into carbon dioxide gas. means for absorbing the carbon dioxide gas into the basic absorption liquid by permeating the carbon dioxide gas through a diaphragm that does not allow ions to pass through, and means for detecting the amount of change in conductivity of the basic absorption liquid. A method for measuring uric acid, characterized in that uric acid in a subject is indirectly measured from a change in the conductivity of the absorption liquid.
および導出される導入口および導出「1が設けられフロ
ーセル内の一側の流路を構成する第1幸と、被検体を含
むキャリアが夫々導入および導出される導入口および導
出口が設けられるとともに前記第1室とけイオンを通さ
ずガスを通す隔膜を介1.て隔てられ月つフローセル内
の他側の流路を構成する第2室と、前記被検体中の尿酸
に作用して酵素反応を生ぜしめるウリカーゼ酵素が固定
化された固定化酵素と、該固定化酵”素から前記第2室
の導入口に至る流路の途中に設けられた試薬添加口と、
前記固定化酵素の入口伺近に設けられ所定側口の被検体
を注入するサンプルインジェクタと、前記第1室の導出
口付近に設けられ前記吸収液の導電率を検出する電極と
な共イ1Iil l/、前記吸収液の導電率変化量から
間接的に被検体中の尿酸を測定することを特徴とする尿
酸測定装置。(2) A first port is provided with an inlet and a lead-out port 1 through which a basic absorption liquid that absorbs carbon dioxide gas is introduced and taken out, respectively, and constitutes a flow path on one side of the flow cell, and a carrier containing an analyte is provided. A second chamber is provided with an inlet and an outlet for introduction and extraction, respectively, and is separated from the first chamber by a diaphragm that does not allow dissolved ions to pass through, but allows gas to pass through, and constitutes a flow path on the other side within the flow cell. a chamber, an immobilized enzyme on which a uricase enzyme is immobilized that acts on uric acid in the specimen to cause an enzymatic reaction, and a flow path from the immobilized enzyme to the inlet of the second chamber. A reagent addition port provided in the
A sample injector is provided near the inlet of the immobilized enzyme and injects the sample from a predetermined side port, and an electrode is provided near the outlet of the first chamber and detects the conductivity of the absorption liquid. l/, a uric acid measuring device that indirectly measures uric acid in a subject from the amount of change in conductivity of the absorption liquid.
および導出される導入口および導出口が設けられ70−
セル内の一側の流路を構成する第1室ど、該第1室の導
出口付近に設けられ前記吸収液の導電率を検出する電極
と、被検体を含むキャリアが夫々導入および導出される
導入口および導出口が設けられるとともに前記第1室と
はイオンを通さずガスを通す隔膜を介して隔てられ且つ
フローセル内の他側の流路を構成−ンる第2室と、該第
2室の導入[1付近に設けられた試薬添加[」とを、前
記固定化酵素の−IM「、に配設するとともに前記サン
プル・インジェクタから注入固定化酵素の」二流および
[流に:l、−tJる2つの電極で検出された前記吸収
液の導電、率変化1t1の差から被検体中の尿酸を測定
する特tt’l請求範囲第(2)項記載の尿酸4(11
定装置a’。(3) An inlet and an outlet are provided through which a basic absorption liquid that absorbs carbon dioxide gas is introduced and extracted, respectively.
In a first chamber constituting a flow path on one side of the cell, an electrode provided near an outlet of the first chamber to detect the conductivity of the absorption liquid and a carrier containing an analyte are introduced and extracted, respectively. A second chamber is provided with an inlet and an outlet, and is separated from the first chamber via a diaphragm that does not allow ions to pass through but allows gas to pass through, and constitutes a flow path on the other side within the flow cell; The introduction of two chambers [1] and the reagent addition provided near 1 are placed in the -IM of the immobilized enzyme, and the immobilized enzyme is injected from the sample injector into the second and [streams: l , -tJ Uric acid 4 (11
Fixed device a'.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13909681A JPS606639B2 (en) | 1981-09-03 | 1981-09-03 | Method and device for measuring uric acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13909681A JPS606639B2 (en) | 1981-09-03 | 1981-09-03 | Method and device for measuring uric acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5840100A true JPS5840100A (en) | 1983-03-08 |
JPS606639B2 JPS606639B2 (en) | 1985-02-19 |
Family
ID=15237397
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP13909681A Expired JPS606639B2 (en) | 1981-09-03 | 1981-09-03 | Method and device for measuring uric acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS606639B2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0313252U (en) * | 1989-06-27 | 1991-02-12 | ||
JPH0544634U (en) * | 1991-11-22 | 1993-06-15 | 河西工業株式会社 | Door armrest mounting structure |
-
1981
- 1981-09-03 JP JP13909681A patent/JPS606639B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS606639B2 (en) | 1985-02-19 |
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