JPS58189003A - Solid-liquid separation of tissue of living organism - Google Patents

Abstract

PURPOSE:To attain to enhance efficiency in solid-liquid separation, by separating an aqueous solution containing an org. component from the tissue of a living organism by an electroendosmosis method in such a state that said tissue is held between diaphragms. CONSTITUTION:As a membrane, there is a hidrophilic neutral membrane or an ion exchange membrane and, as the example of the representative hydrophilic neutral membrane, there are a cellophane, a polivinyl alcohol, a cellulose acetate and a polyamide membranes but it is especially suitable to usually select the same from the ion exchange membrane. As a cation exchange membrane, one obtained from a composition comprising 100-15wt% ethylenic copolymer and 0-85wt% thermoplastic resin inert to a sufonating agent as compared to said copolymer is especially preferred. An electrode chamber or an electrode diaphragm is made movable to be permitted to follow the reduction in the volume of a sample due to water pressure or oil pressure. In a continuous separating method, the electrode diaphragm or the electrode chamber itself is formed into the belt of a conveyor belt.

Description

[Detailed description of the invention] The present invention relates to a method for separating an aqueous solution containing organic components from

In particular, the present invention relates to a novel separation method for separating a solid part and a liquid part containing organic components by electroosmosis.

In general, biological tissue contains a large amount of water, and various organic components are dissolved or dispersed in this water.These separation operations are important in the food industry, pharmaceutical industry, etc. However, it is said to be relatively difficult due to the special properties of biological tissue.There are physical separation methods, diffusion separation methods, etc.As separation methods, there are physical separation methods. , after crushing the solid or gel,
Solid-liquid separation is performed by applying a centrifugal sedimentation method, a centrifugal filtration method, a squeeze p-negligence method, a reduced pressure p-appropriate method, etc. However, 1) as a result of pulverization, the solid becomes fine hydrophilic particles with a strong bond with water (2), and depending on the degree of pulverization, the components may change in quality due to oxidation by oxygen in the air. wake up , 3), The shape of the solid particles is irregular and weak (because it is weak, it causes clogging and compression during filtration and compression, resulting in a decrease in θ and overspeed. 4), the specific gravity is the specific gravity of water. Approximate to −((・ru,
Sedimentation rate decreases and separation becomes difficult. 5) It is easily affected by temperature, and especially in the compression method, which is relatively frequently used in the food industry, ingredients are likely to deteriorate due to the heat generated by high pressure.

There are other problems. On the other hand, in the diffusion separation method, organic components are extracted from biological tissues using hot water, etc., but the rate of separation is extremely slow (・
. Industrially, in order to speed up this process, pretreatment such as crushing or slicing into flakes is performed, but depending on the size of the solid after treatment, it may be difficult to separate the solid from the extract. , and also requires extraction W concentration, etc.
The disadvantage is that the process is quite sloppy.

In addition, as a solid-liquid separation method by electroosmosis, the present applicant has already reported a highly efficient method for dehydrating gel or paste-like materials in JP-A No. 54-76488, JP-A No. 76488, This method involves the use of an ion-exchange membrane with electro-osmotic force, in which most of the dehydrated water is taken into the electrode chamber, resulting in dehydration.

Therefore, the characteristics required of the membrane are: 1) The electroosmotic force is good; 2) The ability to prevent contamination by electrolyte; 3) The membrane itself does not contaminate the target object.
etc., and since only water is separated from the object, there is no need to consider prevention of membrane permeation of organic substances.

The present inventors aimed to expand the scope of application of the dehydration method, and while conducting various studies, they discovered that electroosmosis can be used for solid-liquid separation of biological tissue such as fruit without adding any specific artificial chemical treatment. It was discovered that the present invention can be applied, and the present invention was achieved.

The purpose of the present invention is to avoid adding any artificial chemical treatment.
The object of the present invention is to propose a method for efficiently separating an aqueous solution containing organic components from biological tissues at room temperature, and in particular, without being contaminated by electrolytic products or electrolytes at electrodes.

To explain the present invention, the present invention is a solid-liquid separation method characterized by sandwiching biological tissue between diaphragms and separating an aqueous solution containing organic components from the biological tissue by electroosmosis. .

With this method, it is possible to separate aqueous solutions containing organic components from solid or gel-like biological tissue, which has been considered relatively difficult in the past: 1) without any artificial chemical pretreatment; 2) 39. without deterioration of the target object or organic components due to high temperature or oxidation. 4) It is hardly contaminated by electrolysis products, etc., and 5) It is easy to separate objects (such as bananas) that are extremely difficult to separate using normal methods. Go, 6
), the final preferred one is one in which about 90% of the solution portion can be separated.

The present invention will be explained in more detail below.This invention ii.j
The solid or gel-like biological tissues referred to in -i refer to muscle tissue, nerve tissue, and connective tissue in animals or plants, which are aggregates of cells that are the same shape, the same size, and have similar functions. Especially seafood.

Refers to the meat and organs of animals and birds. In plants, the epidermal tissue
Refers to the soft tissues of plants, especially roots, trunks, branches, flowers, leaves, fruits, seeds, buds, pericarp, and bark.

In general, in electroosmosis, the direction of water movement differs depending on whether the fixed charge is a positive charge or a negative charge, but since biological tissue has a complex structure, the direction of water movement is not so simple (・However, when the aqueous solution containing organic components exits the tissue due to movement by electroosmosis, it drips to the lower part and is thus separated.At this time, the p-overeffect due to the structural support of the biological tissue itself Therefore, relatively high molecular weight starch, protein, etc. remain inside the tissue, and relatively low molecular weight aroma components, sugars, vitamins, enzymes, hormones, amino acids, etc. are separated.

In this case, if an electrode diaphragm is not used, the electrochemical reaction at the electrode will cause a chemical change in the χ·l dye, making it impossible to achieve the desired separation.

The electrode diaphragm used in the present invention is 1).

2) The material is edge cloth, so water movement due to diffusion is small.

3) The membrane itself does not contaminate the object, 3) It is difficult to penetrate the dyeing substance in the electrode liquid chamber, 4) The membrane itself does not contaminate the object, 5)
It has characteristics such as being difficult to penetrate, especially organic substances. If the electroosmotic property of the membrane removal is high, the water flow generated in the membrane will make it difficult for organic substances to flow into the electrode solution chamber, so it is preferable that the electroosmotic property of the membrane itself be low.

When dehydrating a gel or paste-like material using membrane electroosmosis, it is necessary to arrange at least an anion exchange membrane at the anode and/or a cation exchange membrane at the cathode. As long as the film satisfies the characteristics, there is no problem even if the polarity is reversed (in that case,
If the electroosmotic properties of the membrane are human, the electrode solution may flow into the sample chamber and contaminate the sample, which is undesirable (・.

The most common type of sample contamination caused by electrochemical reactions at the electrode is the oxidation reaction at the anode. Even if it has exchangeability (has a negative charge), if the target is food, medicine, etc., chemical changes in the target will be unavoidable unless a cathode diaphragm is used, so it is not desirable. Examples of typical hydrophilic neutral membranes include small ion exchange membranes.

Cellophane, hollyvinyl alcohol, Ill cellulose,
There are polyamides, etc., but it is particularly preferable to choose from membranes generally called ion exchange membranes.

For example, it is a cation exchange membrane in which the ion exchange group is a sulfone group, a carboxylic acid group, a phosphoric acid group, a phosphorous acid group, a phenolic hydroxyl group, a sulfonic acid amide group, a perfluoro tertiary flucol, etc. This is an anion exchange membrane in which the exchange group is a quaternary ammonium base, a 1,2° tertiary amine, a tertiary sulfonium base, a quaternary phosphonium base, or the like.

In addition, there are cation exchange membranes and anion exchange membranes in which these cation exchange groups and anion exchange groups are unevenly distributed within the membrane, amphoteric ion exchange membranes in which cation and anion exchange groups are uniformly distributed, and cation exchange membranes and anion exchange membranes in which cation and anion exchange groups are distributed uniformly. It is a composite ion-exchange membrane that exists in layers, or a mosaic ion-exchange membrane in which positive, negative, and ion-exchange group regions exist in parallel to the thickness direction of the membrane. In addition to the above-mentioned ion exchange groups, other functional groups such as ester groups, amide groups, halogen groups, nitrile groups, acyl groups, phosphate ester groups, and hydroxyl groups may also be included. It is an exchange membrane.

In addition, a combination of two or more of these membranes can also be used as the electrode diaphragm of the present invention.

In particular, as a cation exchange membrane, Japanese Patent Publication No. 51-4]035, Japanese Patent Publication No. 52-29988, USP-3925332
A cation exchange membrane in which a sulfone group is introduced into a specific ethylene-based copolymer proposed in No. 1, etc. is preferable because it has the above-mentioned properties. As a more preferable exchange membrane, the cation exchange membrane already proposed by the present applicant in JP-A-57-36]26 is particularly suitable because it has the above-mentioned characteristics and excellent oxidation resistance. To briefly describe the manufacturing method, 9
7-82 mol% of engineering H1-CH3, R2=-〇〇OR8
, -COOR4 (however, R8-C1 to C5 hydrocarbon groups,
R4 = H1 - C6 hydrocarbon groups, alkali metals and alkali IJ form salts with similar metals, rare earth metals, quaternary ammonium salts such as NH4, carboxylic acid groups such as metal ions other than those listed in -2. 15% by weight or more of an ethylene copolymer or a saponified product thereof with a stoichiometric substance having the structure of
~30% by weight and 85% by weight or less, for example, 5 to 70% by weight of a thermoplastic resin relatively inert to the sulfonating agent. and at least 5 to 200 parts by weight of an extractable plasticizer before, during, or after sulfonation.
After melt-forming into a 200 μm thick film and cooling and solidifying, perform a sulfonation reaction while extracting the plasticizer with a sulfonating agent, or at least partially extract the plasticizer with a solvent before sulfonation, The exchange capacity of sulfo/group is increased by 02 to 4 milliquivalents/
In grams, the electrical resistance in dilute sulfuric acid is 001~2oΩ・6m
2, preferably 0.05 to 50 cm", more preferably '
(is a force of 01 to 1 Ω m2 for a Von exchange membrane.

If the ethylene content of such an ethylene copolymer exceeds 97 mol%, it becomes inactive to the sulfonating agent, prolongs one reaction time, and reduces productivity. If the ethylene content is less than 82 mol%, the film cannot be formed easily by melt forming.
The oxidation resistance of the sulfonated ethylene copolymer is significantly reduced, making it impossible to obtain a good cation exchange membrane. Therefore, an ethylene copolymer having an ethylene content of 97 to 82 mol % is suitable.

In addition, as a thermoplastic resin that is relatively inert to sulfonating agents, its sulfonation reaction is extremely slow compared to the above-mentioned ethylene copolymers, and it can be easily and uniformly mixed using the processing method of the general N Plus Book. , it is easy to obtain a film of uniform quality (
- For example, polyolefin resins such as low-density, high-density poly, ethylene, polypropylene, and polybutene are suitable. The appropriate blending ratio is 85% by weight or less, based on the total weight of the resin composition. If it exceeds 85% by weight, the sulfonation reaction will be slow and the exchange capacity of sulfone groups will be 0.2%.
Continuous production of cation exchange membranes of milliequivalents/gram or more becomes difficult.

A plasticizer that is compatible with and extractable from the resin composition is a plasticizer that is uniformly distributed in an amount of at least 5 parts by weight per 100 weight of the resin composition in the molten state of the resin, and that is formed into a thin film by melt molding. As long as a film can be formed and the resin composition is hardly dissolved (・, and can be extracted from the film or membrane with a solvent or sulfonating agent (・0), for example, polyesters such as diethyl phthalate, dioctyl phthalate, etc. A plasticizer commonly used for vinyl chloride resin, liquid paraffin, is added in an amount of 5 to 2 ml per loomi part of the resin composition.
00 parts by weight is appropriate.

These mixing and film formation are usually carried out by known methods. The appropriate thickness of the film is 5 to 200 μm.

In addition, here (・u) Ions that can form salts with other carboxylic acid groups include, for example, divalent metal ions such as Mg and Cazr12'', and trivalent metal ions such as At''+. Cations that can form salts with carboxylic acid groups such as NH4 are preferred.

In addition, the cation exchange membranes proposed by the present applicant in Japanese Patent Application Laid-open No. 57-40527, Japanese Patent Application No. 15798-1982, and Japanese Patent Application No. 96-96500-1980 have, in addition to the above-mentioned characteristics, durability,
Needless to say, it is particularly suitable as the electrode diaphragm of the present invention because of its excellent productivity, workability, etc.

In the present invention, when free water, which is the source of electroosmotic flow in tissue, is almost completely lost through electroosmotic separation, most of the organic components that can be separated are also separated. At this point, no further separation by electroosmosis can be performed at °C, but unless the free water is increased by somehow adding water to the biological tissue from the outside, electroosmotic flow will occur again and the remaining organic components It also has the characteristic that it can be separated. Furthermore, it is possible to apply a similar separation method to biological tissue that originally has a low water content or is in a semi-dry state by applying pretreatment such as soaking it in water to increase its water content. Needless to say (...Also, after separating free water and water-soluble organic substances, it is treated with organic solvents such as alcohols, ketones such as acetone, aliphatic or alicyclic hydrocarbons such as hexane, and ethers. It is also possible to efficiently extract water-insoluble components.

The present invention can be applied if the target object is biological tissue, but in the case of plants, roots, trunks, branches, flowers, leaves, fruits, seeds, buds, pericarp, juice extracted from bark, non-starchy substances, etc. Removal of unnecessary components, collection of enzymes, vitamins, hormones, aromatic components, etc., collection of extracts, hormones, enzymes, vitamins, etc. from animals (e.g. seafood, meat of animals and birds, organs), removal of odors, etc. , is particularly effective in removing unnecessary non-protein components.

When the present invention is carried out industrially, a large amount of sample is put into the sample tank, but in this case, gaps may be created between the samples or between the sample and the membrane, or the contact area may become small. , electrical resistance may increase. To prevent this, it is necessary to immerse the sample in water or put the sample into the sample tank, and then add water to the surface of the sample so that moisture is present between the samples. After making it possible to apply electricity, excess water is allowed to drip from the separation liquid dripping hole, and then electricity is applied. There are methods such as taking measures to prevent this, or using a combination of these measures.

Generally, in electroosmotic separation, the sample volume decreases as separation progresses, and the contact area between the samples or between the sample and the membrane tends to decrease. It is necessary to take measures to prevent the problem from becoming smaller. For example, the electrode chamber or electrode diaphragm shown in Figure 1 may be made movable to follow the volume reduction of the sample using water pressure, oil pressure, etc., or the apparatus may be configured so that both electrode chambers of the apparatus shown in Figure 1 are at the bottom. There is a method in which the electrode diaphragm is placed horizontally or diagonally so that the soil electrode diaphragm sinks due to gravity as the volume of the sample decreases.

In addition, as a continuous/separation method, the electrode diaphragm or the electrolyte chamber itself is placed between the belts of a belt conveyor, and the object is sandwiched between the belts of two belt conveyors. There are methods, such as installing a conveyor, to reduce the distance between them.

The water content, sulfonic group exchange capacity, and electrical resistance in dilute sulfuric acid used in the present invention are values measured by the following methods.

Moisture content (wt%) (Contains no water of crystallization or compound water that cannot be removed by drying.) (Place a well-washed weighing tube in an electric dryer adjusted to 1 + 60°C, and dry it day and night. After it was left to stand, it was transferred to a tea rack, and after 30 minutes, it was weighed and marked as Wo.

(2) Place 3 to 4 samples of approximately 1 cm square in a weighing tube, weigh them, and define them as Wl.

(3) After leaving it in the electric dryer for a day and night, it was transferred to a tear racker, and after 30 minutes, it was weighed and designated as w2.

(4) (1), +21. From +3'+, the moisture content can be determined by the following formula.

Exchange capacity (milliequivalent/g) A hydrophilized sulfonic acid (-8O8H) type membrane is placed in a certain amount of aqueous solution of chloride (IN) to achieve equilibrium, and the hydrogen chloride generated in the solution is exchanged with 01N. It was titrated with Caseinoder water liquor (potency -r) using phenolphthalein 7 as an indicator (·), and the value X (cc) was divided by the dry type lid W (9) in the potassium salt state. Measuring device (JISC2
3) Set the sample at 23°C between the electrodes (according to 3).
Measure the voltage drop across the sample when a constant DC current with a density of 25 mA/cm2 is applied to the sample, and use the value calculated from the formula 1 as the electrical resistance in dilute sulfuric acid.

R-Electrical resistance of sample in dilute sulfuric acid (Ω・cm”)vl-
Voltage drop (V) when a sample is not set v* = Voltage drop (V) when a sample is set The present invention will be explained in more detail with reference to Examples.

Experimental Example 1 -COOCH3 obtained by saponifying (degree of saponification 60 mol%) and neutralizing (degree of neutralization = 30 mol%) a copolymer of 942 mol% ethylene and 58 mol% methyl methacrylate. ,
-C00H and -COONa group-containing ethylene copolymer (M, I = 1.0) 75% by weight, 2
5% by weight high density polyethylene (density 0,9559/
cm”, Ml = 7) in a kneader, 190
The mixture was kneaded at 190° C. for 30 minutes, and then 43 parts by weight of liquid paraffin (manufactured by Kokusan Kagaku Co., Ltd.) was added to 100 parts by weight of the resin composition, and the mixture was further kneaded at 190° C. for 30 minutes. Next, the above resin mixture was heated in an extruder at a temperature of +80"C, extruded through a Durcular die, and rapidly cooled with water at a temperature of 20°C to form a film with a thickness of 30 μm. Obtained.

Then, the film marked F- was heated to 1.1 at room temperature. ]-)] It was immersed in dichloroethane for about 10 minutes to extract liquid paraffin.

() in fuming sulfuric acid containing 12% free sulfur trioxide, treated at 35"C for 5 minutes, washed in the order of sulfuric acid, diluted sulfuric acid, and water, neutralized with an aqueous potassium hydroxide solution, and then washed with water. It was dried to obtain a cation exchange membrane.

The exchange capacity of this membrane was 215 milliJ equivalent/g, and the membrane had a low electrical resistance of 0.15 Ω·0 m 2 in dilute sulfuric acid.

Experimental Example 2 High-density polyethylene (density -0,9559jcm3゜Ml-
7) 1% of 60.60% by weight was melt-kneaded in a kneader in the same manner as in Experimental Example I, and then 67 parts by weight of liquid paraffin was added to 100 parts by weight of the resin composition and further melt-kneaded.

Next, the resin mixture was extruded using an extruder equipped with a circular die (dice temperature = 150°C) to obtain a film having an original thickness of 25 μm.

Below, extraction of liquid paraffin using a method similar to Experimental Example 1,
A sulfonation treatment was performed to obtain a hydrophilic membrane of 235 meq/g.

The electrical resistance of this hydrophilic membrane in dilute sulfuric acid is 03Ω・0m2
It was extremely low.

Experimental Example 3 Using the apparatus shown in Fig. 1, the S
The same amount of pure water was placed in the wt% sodium sulfate aqueous solution and the liquid chamber 10, and the mixture was allowed to stand at room temperature (22°C). Insert a glass electrode and a Na electrode into the liquid chamber 10 to close the liquid chamber 10) IJ
Changes in ion concentration over time were measured.

The membrane area was 5 cm x 5 cm. the result,
The diffusion coefficient of the Kabuon exchange membrane of Experimental Example 1 for Na in terms of sulfuric acid (IJ) is 5
, Cm''/SeCo In the cation exchange membrane of Experimental Example 2, I x I 0-8 cm2/ULjC, and in the cellophane membrane measured for comparison, 2-5 > I 0-7c
m2///3e (It is known from this that the cation exchange membrane of Experimental Example 1 or 2 is excellent in preventing teta staining of the target object from natural diffusion of electrode solution ions and is suitable as an electrode diaphragm.

Example 1 The apparatus shown in FIG. 2 was used, and the Kabuon exchange membrane of Experimental Example 1 was used as an electrode diaphragm. Effective area is 5 cm x 5 cm
It is. Carbon plates were used for both electrodes (and a 1 wt % sulfuric acid aqueous solution was used for both electrode chamber solutions. Between the membranes was a Japanese radish with a thickness of I cm <259
．． Moisture content: 93% by weight) with scissors and 20 V/c+n ("
Apply a constant voltage of F average current 20 mA 7 cm2),
Electricity was applied for 30 minutes. After energization, the weight of the radish was 49%, the water-soluble solid content in the separated liquid was 5°Bzx (Br+x refractometer), and the radish was in the form of a sheet with a thickness of about 1 dragon and was easy to handle. .

Efficiency: 4 kg of separated liquid can be obtained with 1 kW of electricity,
It was extremely energy efficient.

Comparative Example 1 After pulverizing radish, suction filtration was performed to obtain p liquid. The water-soluble solid content in the U liquid was 6°Br+x, which was the same as that of the separated liquid obtained in Example 1.

Example 2 The radish was changed to 269 banana (moisture content 74% by weight),
A separation experiment was conducted in the same manner as in Example I, except that the cation exchange membrane of Experimental Example 2 was used as an electrode diaphragm. After energization, the banana weighed 9 g (moisture content: 28% by weight), and 17 g of the separated liquid was transparent, slightly colored, and emitted a strong aroma. The water-soluble solid content was 18°Br+x. According to the gas chromatograph, the separated liquid had the same peak as the p liquid of Comparative Example 2, and the energy efficiency was good at 3 kg/kwh.

Comparative Example 2 Bananas were subjected to the same treatment as in Comparative Example 1. The water-soluble solid content was 20°Br1x, which was the same value as in Example 2.

However, filtration was extremely difficult, and only a small amount of liquid F was obtained, and the taihei remained in a pasty state.

Comparative Example 3 A banana with a thickness of 50IX cm (thickness cut into pieces) was directly sandwiched between electrode plates and energized. Bubbles were generated violently at the contact surface between the banana and the electrode plate, so when we separated them, a part of the banana turned brown and adhered to the surface of the electrode plate [7. The surface of the banana also changed in quality and turned brown. .

Example 3 A separation experiment was conducted in the same manner as in Example 2, except that the applied voltage was changed to 10 V, /c, n. Banana after electricity is 1
49, and the energy efficiency was 7 kg/i<wb, which was a high efficiency [tJ]. Water-soluble solid content is 19゜Br
There is sufficient separation even when the voltage is reduced.

Example 4 A separation experiment was conducted in the same manner as in Example 1, except that the radish was replaced with 229 carrots (moisture content: 93% by weight) and the current application time was changed to 15 minutes. After electricity is applied, the carrot becomes 139, the separated liquid is clear and slightly reddish, and the water-soluble solid content is 6°B.
r]X, the energy efficiency was 2 kg/kwb.

Comparative Example 4 Carrots were treated in the same manner as in Comparative Example 1. The water-soluble solid content in the P solution was 9°Br1x, and a large amount of orange precipitate was suspended, making it opaque and unable to be filtered with ordinary paper.

Example 5 A separation experiment was conducted in the same manner as in Example 1, except that radish was replaced with 219 potato (moisture content: 73% by weight). After energization, the potato weighed 9 g.

Only non-starchy organic components are present in the separated liquid,
Starch was separated from other components in solid form.

Example 6 The same process as in Example 4 was carried out except that 26 g (water content 74% by weight) of carrots was used (water content 5%).
2% by weight banana was obtained. Furthermore, a separation experiment similar to that in Example 4 was conducted using the banana. 59 separation liquid was obtained, and the energy efficiency was 15X? Separation was possible even from the Awh and low water content. The moisture content of the banana finally obtained was 25% by weight, and 90% of the liquid content in the banana was separated.

Example 7 An experiment was conducted in the same manner as in Example 2, except that 259 beef thighs (raw) were used instead of bananas. After energization, the weight was 8 g. Water-soluble organic components were separated in the separated liquid. Furthermore, when the separated meat was vacuum dried, delicious dried meat was obtained. Energy efficiency was 3 kli'/kwb.

Example 8 An experiment was conducted in the same manner as in Example 2, except that banana was replaced with 219 medicinal carrot. Carrot weighs 9g after energizing
It became. Most of the liquid content of medicinal ginseng was separated. Energy efficiency was 2 kvkwh.

[Brief explanation of drawings]

Figure 1 is an explanatory diagram showing an apparatus used for measuring the diffusion coefficient of a membrane (Experimental Example 3), and Figure 2 is an explanatory diagram showing an apparatus used for measuring the diffusion coefficient of a membrane (Experimental Example 3).
FIG. 1. Anode, 2. Cathode, 3. Diaphragm, 3a. Anode diaphragm, 3b. Cathode diaphragm, 5. Biological tissue, 6 Positive applicant Asahi Dow Co., Ltd.

Claims (1)

[Scope of Claims] m. A solid-liquid separation method characterized by sandwiching biological tissue between membranes and separating an aqueous solution containing organic components from the biological tissue by electroosmosis. (2) The separation method according to claim 1, wherein the anode side diaphragm is at least a cation exchange membrane. (3) Said II (On-exchange membrane is 97 to 82 mol%
H8, R2 = 0COR8, -cooR4 (However, R8
201-C6 hydrocarbon group, R4=H, C, -C6 hydrocarbon group, alkali metals, ions that can form salts with other carboxylic acid groups)] Ethylene with a monomer having the structure Patent for an ion exchange membrane having a sulfonic group with an exchange capacity of 0.2 to 4 milliequivalents/gram obtained from a copolymer or a saponified product thereof, and an electrical resistance of 0.01 to 20 Ωcm2 in dilute sulfuric acid. A separation method according to claim 2. (4) Electrical resistance in dilute sulfuric acid is 0.05-5Ω・cm
2. The separation method according to claim 3, which is f5+ ethylene copolymer 100-15 wt%,
According to claim 3 or 4, which is an ion exchange membrane obtained from a resin composition containing 0 to 85 wt% of a thermoplastic resin that is inactive to the sulfonating agent □ compared to the ethylene copolymer. Separation method as described. (6) The separation method according to claim 5, wherein the thermoplastic resin is selected from the group consisting of polyethylene, polypropylene, and polybutene-1. (7) The separation method according to claim 1, wherein the biological tissue is a plant tissue. (8) The biological tissue is a plant root, branch, flower, leaf, fruit,
The method for separating seeds, buds, pericarp, and bark according to claim 7. (9) The separation method according to any one of claims 1 to 6, wherein the biological tissue is an animal tissue. 0) The separation method according to claim 9, wherein the animal body tissue is seafood, animal or poultry meat, or animal or poultry organs.