JPS58167949A - Diaphragm type oxygen concentration measuring method and diaphragm type oxygen electrode - Google Patents

Abstract

PURPOSE:To make the replacement of an oxygen electrode easier by such an arrangement wherein a part of a tank to be inspected is composed of a sheet of oxygen transmission membrane and a diaphragm type oxygen electrode is installed in close proximity to the oxygen transission membrane. CONSTITUTION:An oxygen transmission membrane 3 is fixed to an electrode holder 10 by means of an O-ring 2, and the holder is slid and fixed to the nozzle 1 of a flask, which is a tank to be inspected, by means of another O-ring 4. If a flange 6 is screwed into outer threads 5 provided on the holder, it can be used as a guide for inserting the electrode holder. Under this condition, the flask is steam sterilized, and after that, a diaphragm type oxygen electrode is inserted into the holder, and the diaphragm of the electrode is caused to closely contact with the oxygen transmission membrane by screwing a cap nut 8 into the outer thread 5. By such a composition as stated above, there will be less possibility that the electrode may be damaged and it becomes possible to replace the electrode easily.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides ili! This article relates to the structure of an IlI type oxygen electrode and a method for measuring oxygen concentration using the same, and the oxygen electrode can be easily converted by using an oxygen permeable membrane between the test object and the oxygen electrode9. In addition, the vapor film 1 of the test tank can be formed safely and easily.

Conventionally, when measuring the degree of oxygen in the gas or liquid phase in a tank, a diaphragm-type oxygen electrode was often attached directly to the test tank. However, when the electrode breaks down, it is difficult to replace the electrode because the liquid or gas in the test tank leaks from the electrode attachment part. When fixed, once the electrode is removed,
Therefore, it is desirable to be able to replace the electrodes without affecting the subject.

When using an oxygen electrode for fermentation, it is necessary to steam sterilize the electrode while it is attached to the II# tank.
Harsh conditions such as pressure resistance and humidity resistance were required. Furthermore, in measuring dissolved oxygen in a shaking flask, the electrode is also shaken at the same time, so in addition to the above conditions, the electrode is also required to be small and light. In addition, such electrodes are said to have a cylindrical structure that does not protrude inward from the flask wall to avoid the negative effects of stirring.

Neither an oxygen electrode nor a measurement method that satisfies these conditions has yet been obtained.

In order to solve these problems, the present invention provides a new diaphragm type oxygen electrode and a method for measuring 11111A performance using the same.

That is, in the present invention, a part of the tank wall of the test tank that comes into contact with the test liquid or gas is constituted by an oxygen permeable membrane, a diaphragm type oxygen electrode is attached to the outside of the tank 1111, and the diaphragm is connected to the oxygen permeable membrane. This method measures the oxygen concentration in the test tank by bringing it into close contact with the test tank.

Furthermore, in the method of the present invention described above, an oxygen permeable membrane and 14
By interposing a liquid between the membrane and the membrane, measurement accuracy can be increased. Silicone oil as a liquid
Other oils, ethylene glycol, petrolatum, etc. may be used. When 1I4JIK comes into close contact with the oxygen permeable membrane, this liquid is present around the contact point between the two, forming an oxygen barrier that prevents oxygen from entering toward the outer circumference of the electrode, and at the same time fills the gap between the two. Fill.

In practice, the purpose is achieved by applying a small amount of liquid to the tip of the electrode and attaching it tightly to the oxygen permeable JilK.

As stated above, the test tank equipped with an oxygen permeable membrane and the diaphragm type oxygen electrode are structurally separated.

Utilizing this, it is possible to attach a diaphragm-type oxygen electrode to the test tank after steaming [11]. Also, when performing this steam membrane 1, an oxygen permeable membrane can be attached to the outside to withstand the internal pressure. It is also possible to support and fix it with a presser. If the electrodes are attached after the vapor film is finished, they do not have to be exposed to harsh sterilization conditions.

The present invention also includes a diaphragm-type oxygen electrode characterized in that the diaphragm is attached in close contact with the oxygen permeable membrane fixed to the test tank used in the method described above. Embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

Fig. 1 shows the electrodes attached when measuring dissolved oxygen in a shake flask, Fig. 2 shows the factors involved in attaching the oxygen permeable membrane to the electrode holder, and Fig. 5 shows the electrodes. The procedure for assembling this is as follows: electrode $le/-C1o>ni, 0-ring (2
) IIc yacht, fix the oxygen permeable membrane (3) and attach it to the flask nozzle (1) with another O-ring (4).
) is fixed to nitm. 2ko00-ring (2),
(4) Using K, fix the nozzle and holder tightly together.

At this time, the external screw (5) and K7 lunge (6) attached to the holder
If you screw them together, you can use it as a guide for the position to insert the electrode holder, and it is convenient to attach and remove it from the sushi @ no 1 handle.

In this state, after steam sterilizing, insert the diaphragm type oxygen electrode (7) into the holder and tighten one bag nut (8) to the external screw of the holder to make the electrode's diaphragm (9) and oxygen permeable membrane come into close contact. In this way, there is no need to heat sterilize the electrodes, so there is less risk of damaging the electrodes, and even if the electrodes fail, there is an advantage that the electrode tube can be replaced without affecting the measurement system. When using a steam membrane in a fermentation tank of 501 or more, if one oxygen permeable membrane is installed as described above, the oxygen permeation will be 11.
There is a risk of damage due to pressure being applied to 4.

Therefore, when steam @ germs, instead of the electrode, insert a presser rod whose tip contacts the oxygen permeable membrane, kill the bacteria, pull out the presser rod, and insert a diaphragm type oxygen electric lit. This eliminates the need to apply pressure to the membrane K. This presser rod preferably has the same diameter and shape as the oxygen electrode to be inserted.

[Brief explanation of the drawing]

The drawings provide a detailed explanation of the invention. FIG.
Due to leakage factors, Fig. 2 shows a diagram of the oxygen permeability mVt attached to the electrode holder, and Fig. 5 shows a diagram of the electrode. In the figure, 5 indicates an oxygen permeable membrane, and 7 indicates a diaphragm type oxygen electrode. Patent Applicant: Ishikawa Seisakusho Co., Ltd. Procedural Amendment 11 (Voluntary) June 13, 1980 1, Incident Indication % Application No. 57-51617 2 Title of Invention Diaphragm-type Oxygen Concentration Measuring Method and Diaphragm-Type Oxygen Electrode 4 Amendment Relationship with the case of the applicant Patent applicant 4: Application subject to amendment and Mei + 11B document i Contents of amendment 1: I will print out the application as attached. ) 4 (Sleeve king 1 (Oiri) murmur 11047) 127I8 Calendar + f8f Hair 2° ☆ 5 I many I 0 Su spoon? west l
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Claims (1)

[Claims] 1. A part of the wall of the test tank that comes into contact with the test liquid or gas is constituted by an oxygen-permeable membrane, and a diaphragm-type oxygen electrode is attached to the outside of the tank,
Because the diaphragm is in close contact with the oxygen permeable membrane,
Method for measuring oxygen 11K in IL paddles. 2- The method as set forth in claim 1, characterized in that a mounting member is interposed between the oxygen permeable membrane and the I'A membrane. 812. The method according to claim 811, wherein the tank to be tested is a fermentation tank, and a V4 lug-type oxygen electrode is attached after the steam membrane is heated. 3. The method according to claim 3, characterized in that the oxygen permeable membrane is supported and fixed with a presser from the outside so as to withstand internal pressure when performing steam and ddlli. A diaphragm-type oxygen electrode characterized by a diaphragm attached in close contact with an oxygen permeable membrane fixed to a test tank.