JPH037064B2 - - Google Patents
Info
- Publication number
- JPH037064B2 JPH037064B2 JP57140528A JP14052882A JPH037064B2 JP H037064 B2 JPH037064 B2 JP H037064B2 JP 57140528 A JP57140528 A JP 57140528A JP 14052882 A JP14052882 A JP 14052882A JP H037064 B2 JPH037064 B2 JP H037064B2
- Authority
- JP
- Japan
- Prior art keywords
- gas
- diaphragm
- electrode
- permeable membrane
- gas permeable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012528 membrane Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 5
- 239000003779 heat-resistant material Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 238000013459 approach Methods 0.000 claims 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 14
- 229910002092 carbon dioxide Inorganic materials 0.000 description 7
- 239000001569 carbon dioxide Substances 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/404—Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid, e.g. Clark-type oxygen sensors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【発明の詳細な説明】
本発明は、発酵工業等において、発酵槽内およ
びガス排気管路内等のガス濃度の側定に使用され
る隔膜式ガス電極の支持装置に関するものであ
る。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a support device for a diaphragm-type gas electrode used in the fermentation industry to determine the gas concentration inside a fermenter, a gas exhaust pipe, and the like.
一般に発酵工業等において、培養中の発酵槽内
の培養液中に溶在する炭酸ガスおよび排気ガス中
の炭酸ガスの濃度の測定することは、培養時にお
ける状態の観察上、重要である。 Generally, in the fermentation industry, it is important to measure the concentration of carbon dioxide gas dissolved in the culture solution in a fermenter during cultivation and the concentration of carbon dioxide gas in exhaust gas in order to observe the state during cultivation.
従来、培養中の発酵槽、排気ガス中の炭酸ガス
濃度の測定には、非分散赤外線式炭酸ガス濃度計
が多く使用されてきたが、装置が高価であるこ
と、排気ガス中に含まれる水蒸気が赤外線を吸収
するため、その影響を補正するなどの対策が必要
であること、ならびに測定セルが汚れ易く保守に
手数を要するほか、この方式では原理的に発酵槽
内の培養液中の溶存炭酸ガスの濃度を、直接測定
することができない等の問題点がある。 Conventionally, non-dispersive infrared carbon dioxide concentration meters have often been used to measure the carbon dioxide concentration in fermenters and exhaust gas during culture, but the equipment is expensive and the water vapor contained in the exhaust gas is often used. absorbs infrared rays, so it is necessary to take measures to compensate for the effects of this, and the measurement cell is easily contaminated and maintenance is troublesome. There are problems such as the inability to directly measure the concentration of gas.
隔膜式電極法による発酵工程中における排気ガ
ス中の炭酸ガス濃度、あるいは培養液中の溶存炭
酸ガス濃度の測定は従来からも種々試みられてい
たが、工程中の蒸煮滅菌操作で、電極が高温に曝
される結果、検出部のガラス電極のガラス膜から
溶出するアルカリ分によつて電極内部にあるガス
吸収液がアルカリ性に変質するばかりでなく、検
出用ガラス電極や比較電極の劣化が促進されるた
め、測定は容易でなく、精度も良くないなど十分
な成果をあげることができなかつた。 Various attempts have been made to measure the carbon dioxide concentration in the exhaust gas or the dissolved carbon dioxide concentration in the culture solution during the fermentation process using the diaphragm electrode method, but the electrodes are exposed to high temperatures during the steaming sterilization process during the process. As a result of exposure, the gas absorption liquid inside the electrode becomes alkaline due to the alkali content eluted from the glass film of the glass electrode of the detection part, and the deterioration of the detection glass electrode and reference electrode is accelerated. Therefore, measurement was not easy and the accuracy was not good, so it was not possible to achieve sufficient results.
本発明は上記問題を解決し、発酵槽が高温、高
圧で蒸煮滅菌操作がされる場合にも発酵槽やその
排気管の通路に、隔膜式ガス電極を取付けて使用
しうるようにした電極支持装置を供することを目
的とする。 The present invention solves the above problems and provides an electrode support that allows a diaphragm type gas electrode to be attached and used in the passage of the fermenter and its exhaust pipe even when the fermenter is sterilized by steaming at high temperature and high pressure. The purpose is to provide equipment.
本発明はこの目的を達するために、被検ガスを
透過し耐熱性を有するガス透過膜と、ガス透過膜
と間隔を有しガス透過膜より外側に配置された被
検液被検ガスに侵されない材料からなる多孔性シ
ートとで一端側の開口が閉じられ、他端側の開口
から隔膜式ガス電極を挿入し隔膜式ガス電極の一
端面に形成された隔膜が前記ガス透過膜に接近し
た状態に隔膜式ガス電極を保持しうるように形成
された耐熱耐圧性の材料からなる筒と、前記隔膜
式ガス電極の外形が同一の模型とから隔膜式ガス
電極の支持装置を構成したものである。 In order to achieve this object, the present invention includes a gas-permeable membrane that permeates a test gas and has heat resistance, and a test liquid that penetrates the test gas and is disposed outside the gas-permeable film with a space between the gas-permeable film and the gas-permeable film. The opening at one end was closed with a porous sheet made of a non-porous material, and a diaphragm-type gas electrode was inserted through the opening at the other end, so that the diaphragm formed on one end surface of the diaphragm-type gas electrode approached the gas permeable membrane. A support device for a diaphragm gas electrode is constructed from a cylinder made of a heat-resistant and pressure-resistant material that is formed to hold the diaphragm gas electrode in a stable state, and a model having the same external shape as the diaphragm gas electrode. be.
本発明装置を一実施例について説明すると、１
はステンレス鋼管などの耐圧耐熱性の材料からな
る筒で、その一端側の開口は、被検ガス、例えば
炭酸ガスを選択的に透過する例えばシリコーンゴ
ム膜からなるガス透過膜２と、ガス透過膜２と僅
かな間隔を置いてガス透過膜２より外側に配置さ
れたステンレス網のような被検液、被検ガスに侵
されない十分な通気性と強度を有する多孔性シー
ト３によつて閉じられている。４はガス透過膜２
と多孔性シート３とを筒１の一端に取付けた袋ナ
ツトである。 To explain one embodiment of the device of the present invention, 1
is a cylinder made of a pressure- and heat-resistant material such as a stainless steel pipe, and the opening at one end is connected to a gas-permeable membrane 2 made of, for example, a silicone rubber membrane that selectively permeates the gas to be detected, such as carbon dioxide, and a gas-permeable membrane The sample liquid is placed outside the gas-permeable membrane 2 at a slight distance from the gas permeable membrane 2, and the sample liquid is closed by a porous sheet 3 that has sufficient air permeability and strength to be uncorrupted by the sample gas. ing. 4 is gas permeable membrane 2
This is a cap nut with a porous sheet 3 attached to one end of a tube 1.
筒１は内部に第２図に示すように隔膜式ガス電
極１０を他端から挿入し、その一端面に形成され
た隔膜１１がガス透過膜２に近接した状態で袋ナ
ツト５等により保持できるように形成されてい
る。 The diaphragm-type gas electrode 10 is inserted into the cylinder 1 from the other end as shown in FIG. 2, and the diaphragm 11 formed on one end surface can be held by a cap nut 5 or the like in a state close to the gas permeable membrane 2. It is formed like this.
第１図に示す６は第２図に示した隔膜式ガス電
極１０と外形を同一に形成した模型であつて、筒
１内に隔膜式ガス電極１０と同様に袋ナツト５あ
るいは盲蓋１２によつて保持できるようになつて
いる。 Reference numeral 6 shown in FIG. 1 is a model having the same external shape as the diaphragm gas electrode 10 shown in FIG. It can be twisted and held.
筒１は例えば外周ほぼ中央に設けたフランジ８
によつて発酵槽の壁７等に設けた孔から内部に一
端側を挿入し袋ナツト９によつて壁７に固定して
用いられる。 For example, the tube 1 has a flange 8 provided approximately at the center of the outer circumference.
It is used by inserting one end side into the inside of the fermenter through a hole provided in the wall 7 etc. of the fermentation tank and fixing it to the wall 7 with a cap nut 9.
発酵槽等を高温高圧で殺菌を行なうときには、
第１図に示すように模型６を筒１内に挿入して、
安全のために盲蓋１２で模型６を筒１内に固定し
て滅菌操作を行なう。滅菌操作後は模型６を第２
図のように隔膜式ガス電極１０に取換え発酵操作
時における測定を行なうものである。 When sterilizing fermenters etc. at high temperature and pressure,
As shown in FIG. 1, insert the model 6 into the cylinder 1,
For safety, the model 6 is fixed in the cylinder 1 with a blind lid 12 and sterilization is performed. After the sterilization operation, move model 6 to the second
As shown in the figure, a diaphragm type gas electrode 10 is used to perform measurements during fermentation operations.
このようにして滅菌操作を行なうと、壁７内の
圧力は多孔性シート３を通してガス透過膜２に加
わるが、内側に模型６があつて、ガス透過膜２を
支えるので、内圧に対してガス透過膜２が破損す
ることを防止できる。 When the sterilization operation is performed in this way, the pressure inside the wall 7 is applied to the gas permeable membrane 2 through the porous sheet 3, but since the model 6 is placed inside and supports the gas permeable membrane 2, the pressure inside the wall 7 is applied to the gas permeable membrane 2. It is possible to prevent the permeable membrane 2 from being damaged.
加熱加圧後、壁７内から、加圧気体を排出する
と、壁７内の温度低下に伴つて、筒１内より壁７
内の方の内圧が低くなることがある。このときは
ガス透過膜２が壁７内に吸引されるようになる
が、外側に多孔性シート３が配置されているの
で、これによつてガス透過膜２が支持されること
により、吸引によるガス透過膜２が保護される。 After heating and pressurizing, when the pressurized gas is discharged from inside the wall 7, as the temperature inside the wall 7 decreases, the wall 7 is released from inside the cylinder 1.
The internal pressure inside may become low. At this time, the gas permeable membrane 2 will be sucked into the wall 7, but since the porous sheet 3 is placed on the outside, the gas permeable membrane 2 will be supported by this, and the suction will Gas permeable membrane 2 is protected.
多孔性シート３としてはステンレス網の外、多
孔性プラスチツクやプラスチツク製の網等を用い
ることができる。網の場合、20メツシユより細か
い網目のものがよく、ステンレス鋼線網では200
〜300メツシユ程度のものがよい。 As the porous sheet 3, other than stainless steel mesh, porous plastic, plastic mesh, etc. can be used. In the case of mesh, it is better to have a mesh finer than 20 mesh, and for stainless steel wire mesh it is better to have a mesh of 200 mesh or finer.
~300 meters is best.
ガス透過膜２と多孔性シート３との間に間隙を
設けたのは、密着していると圧力が急に掛つた場
合、多孔性シート３の孔または網目にガス透過膜
２が押し込まれ、接触部分が切れることがあるた
めであるが、間隙が大き過ぎると隔膜式ガス電極
１０を第２図のように保持せしめて発酵状況等の
測定を行なう際、指示応答が遅くなるほか、圧力
に対する保護効果も減少するので、間隙の最大限
度は１mm位である。 The reason for providing a gap between the gas permeable membrane 2 and the porous sheet 3 is that if pressure is suddenly applied when the gas permeable membrane 2 and the porous sheet 3 are in close contact, the gas permeable membrane 2 will be pushed into the holes or meshes of the porous sheet 3. This is because the contact part may break, but if the gap is too large, the indication response will be slow when holding the diaphragm gas electrode 10 as shown in Figure 2 to measure the fermentation status, etc. Since the protective effect is also reduced, the maximum gap is around 1 mm.
本発明装置を用いることにより、隔膜式ガス電
極は、発酵槽等の加熱滅菌操作時に加熱加圧を受
けることなく使用できるので、加熱滅菌操作によ
り電極が劣化をうけることなく精度を維持でき、
ガス透過膜２によつて内外を遮断して発酵槽等の
内部に雑菌や異物の侵入を防止できる。 By using the device of the present invention, the diaphragm gas electrode can be used without being heated and pressurized during heat sterilization of fermenters, etc., so the accuracy can be maintained without deterioration of the electrode due to heat sterilization.
The gas permeable membrane 2 isolates the inside and outside and prevents bacteria and foreign matter from entering the inside of the fermenter.
また模型６と多孔性シート３とにより、ガス透
過膜２を圧力に対し保護できるようにしたので、
ガス透過膜２として応答が早く精度の良いものを
使用でき、感度精度のよい測定ができる。 In addition, the model 6 and the porous sheet 3 protect the gas permeable membrane 2 from pressure.
As the gas permeable membrane 2, a membrane with quick response and high precision can be used, and measurement with high sensitivity and precision can be performed.
第１図、第２図は共に本発明による電極支持装
置を発酵槽等の壁に取付けた状態の縦断面図で、
第１図は模型を挿入し滅菌操作を行なう状態の、
第２図は隔膜式ガス電極を挿入した測定状態の図
である。
１……筒、２……ガス透過膜、３……多孔性シ
ート、４，５……袋ナツト、６……模型、７……
壁、８……フランジ、９……袋ナツト、１０……
隔膜式ガス電極、１１……隔膜、１２……盲蓋。
FIG. 1 and FIG. 2 are both longitudinal cross-sectional views of the electrode support device according to the present invention attached to the wall of a fermenter, etc.
Figure 1 shows the model being inserted and sterilized.
FIG. 2 is a diagram of a measurement state in which a diaphragm type gas electrode is inserted. 1... cylinder, 2... gas permeable membrane, 3... porous sheet, 4, 5... bag nut, 6... model, 7...
Wall, 8...flange, 9...bag nut, 10...
Diaphragm type gas electrode, 11...diaphragm, 12...blind lid.
Claims (1)
と、ガス透過膜と間隔を有しガス透過膜より外側
に配置された被検液被検ガスに侵されない材料か
らなる多孔性シートとで、一端側の開口が閉じら
れ、他端側の開口から隔膜式ガス電極を挿入し隔
膜式ガス電極の一端面に形成された隔膜が前記ガ
ス透過膜に接近した状態に隔膜式ガス電極を保持
しうるように形成した耐圧耐熱性の材料からなる
筒と、前記隔膜式ガス電極と外形が同一の模型と
からなる隔膜式ガス電極支持装置。1. A gas permeable membrane that allows the test gas to pass through and has heat resistance, and a porous sheet made of a material that is not affected by the test liquid and test gas and is placed outside the gas permeable film with a space between the test gas and the gas permeable film. , the opening on one end side is closed, and the diaphragm gas electrode is inserted through the opening on the other end side, and the diaphragm gas electrode is held in a state where the diaphragm formed on one end surface of the diaphragm gas electrode approaches the gas permeable membrane. A diaphragm-type gas electrode support device comprising a cylinder made of a pressure- and heat-resistant material and a model having the same external shape as the diaphragm-type gas electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57140528A JPS5930057A (en) | 1982-08-13 | 1982-08-13 | Diaphragm type gas electrode support apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57140528A JPS5930057A (en) | 1982-08-13 | 1982-08-13 | Diaphragm type gas electrode support apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5930057A JPS5930057A (en) | 1984-02-17 |
| JPH037064B2 true JPH037064B2 (en) | 1991-01-31 |
Family
ID=15270759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57140528A Granted JPS5930057A (en) | 1982-08-13 | 1982-08-13 | Diaphragm type gas electrode support apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5930057A (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4601203A (en) * | 1985-07-01 | 1986-07-22 | Atlantic Richfield Company | Oxygen content sampling system |
| EP0952448B1 (en) * | 1998-04-23 | 2004-03-24 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co.KG. | Sensor holder arrangement for measuring fluids in processing |
| US20090324780A1 (en) | 2008-06-27 | 2009-12-31 | Mars, Incorporated | Dimpled Food Product |
| CA2910546C (en) | 2013-05-14 | 2023-03-28 | Mars, Incorporated | Joint care composition |
-
1982
- 1982-08-13 JP JP57140528A patent/JPS5930057A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5930057A (en) | 1984-02-17 |
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