JPS58149676A - Culture medium - Google Patents

Abstract

PURPOSE:To utilize the burnt ash of animal excrement which is a waste available from stock farms, as an inorganic source of a medium for the cultivation of microorganisms. CONSTITUTION:Excrement of cattle or poultry, such as chicken, pig, cattle, horse, etc. is burnt at >=800 deg.C, and the ash is used as an inorganic source of a culture medium. The inorganic source is compound with proper carbon sources, nitrogen sources and other nutrient components. The amount of the burnt ash is usually 1-50g per 1l of the culture liquid.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a culture medium containing burned ash of livestock manure.

The livestock industry is showing growth over the years due to the improvement of modern eating habits. However, this expansion of the livestock industry, combined with the rapid development of urbanization and the emergence of mixed-living societies, has caused problems such as bad odors and water pollution in various places. This causes environmental pollution, and is causing major social problems as so-called livestock pollution. One of the major causes of environmental pollution related to livestock farming is that an appropriate method has not yet been established for handling the large amounts of manure produced by large-scale intensive livestock farming such as pig and poultry farming. Can be mentioned.

Under this current situation, recently there has been developed an apparatus for incinerating chicken manure at high temperatures11. The so-called **boiler has been developed and is gradually showing signs of becoming popular, especially in large-scale poultry farms. This is a chicken manure boiler.

(11) Chicken manure tubes can be incinerated and the generated heat can be used to heat the floor of the poultry house, replacing most of the conventional heavy oil combustion and greatly saving energy.

(2+ Chicken manure can be easily incinerated because it is dried using the heated floor of the Q house, which is preferable from the standpoint of environmental conservation.

(3) Since the incineration temperature reaches 11,800 to too-0'CK and the organic matter is completely decomposed and burned, no odor is generated compared to conventional processing methods such as drying and composting.

It is expected that it will become more and more popular in the future.

In addition, livestock manure incineration equipment such as Jin is currently limited to processing chicken manure, which has a relatively low moisture content, but if it is used in conjunction with preliminary drying in the sun, it can produce livestock manure, pig manure, cow manure, etc. It is also possible to incinerate horse manure and other livestock feces.
The scope of its use is expected to expand with improvements to the currently developed device.

The 1'IK Otaru Livestock Manure Burnt Ash and #'i Chicken Manure, Pig Manure, Cow Manure, Horse Manure, etc. are the fire ash produced by incinerating livestock and poultry tubes.

When livestock manure is processed using an incinerator like this.

Incinerated residue (hereinafter referred to as noble burned ash) equivalent to about 10 - (weight) of livestock manure is generated. This is a light, grayish-white powder, and because it is fired at high temperatures, it does not have the unpleasant odor characteristic of animal manure (although it is very alkaline, so if it is treated properly, it can be There is an inherent danger that the dumping could cause water pollution and become a new source of pollution.Therefore, the conventional method of using burned ash has not been implemented as a major problem.

As part of our research to find a way to effectively utilize such noble calcined ash, the inventors of the present invention have researched its rich and well-balanced inorganic composition for living organisms (as an example of noble calcined ash, poultry excrement calcined ash) (The inorganic component composition is shown in Table 1) We focused on K and conducted research to utilize it as an inorganic source for microbial culture media.

*-I IIm Example of inorganic component analysis of fecal calcined ash Cultivation of fungal organisms KFi, as a general rule, rounding that causes the growth of microorganisms, *
Takenori, nitrogen source, minerals, trace amounts of vitamins, amino acids,
Additionally, biotin-based ingredients are required. And as for the above-mentioned inorganic substances.

In general, the main components are phosphoric acid, potassium, calcium, and magnesium, and depending on the type of microorganism, iron.

Trace elements such as manganese, zinc, and copper may be required as essential components.

The noble calcined ash according to the present invention contains all of the above-mentioned inorganic components as shown in Table 1 above, and the content and ratio thereof are those used for the growth of normal microorganisms. The ash is used as the mineral source, and the longitudinal cable I1. A good culture medium for microorganisms can be prepared by adding a nitrogen source and a small amount of trace ingredients (tm).This WPFi was completed as a result of research based on the above knowledge, and is a major success. This will be explained in more detail below.

The noble calcined ash used in the present invention is as described above.

This is incineration residue obtained by burning livestock feces at a temperature of 800°C or higher using an incinerator, and does not contain any combustible organic matter. Chicken manure is used in the pre-allocated precious calcined ash.

This includes burned ash of pig manure, cow manure, horse manure, and other livestock manure, but especially in broiler chicken farms, where the floor surface is usually heated, the chicken manure discharged is easily susceptible to IIL and has little variation in composition. Therefore, ash is advantageous in that there is little variation in the content of inorganic components and a large amount of stable quality can be obtained.

Two examples of the culture medium according to the present invention are d: noble calcined ash is preliminarily mixed with the required amount (approximately neutralization equivalent) of sulfuric acid, hydrochloric acid, nitric acid, and phosphoric acid.

Acetic acid and other inorganic or organic acids (including mixed acids)
The inorganic components are solubilized by treatment, and after diluting this appropriately, carbon sources, nitrogen sources, and other O nutrient components are added. It can be prepared by a method such as 2, in which noble calcined ash is dissolved and suspended in water with other nutritional components and the above-mentioned acids are used to adjust the specified espnFc. Especially in the culture medium according to the latter method,
Calcium legate, a component of the burned ash, remains partially undissolved in the culture solution, which prevents the pH from decreasing due to organic acids produced by organisms when they retire from culture, and lowers the pH of the medium during culture.
Effect 4, which stabilizes , is also reduced. The amount of noble calcined ash to be used varies depending on the type of microorganism and cultivation method, but it is preferably used in the range of 1 to 50 tons per 11 of the normal culture solution.

The culture medium prepared as described above is sterilized by conventional methods, and then microorganisms are inoculated and cultured.
Any conventionally known method can be applied to this, but special care must be taken! ! There isn't.

Although the culture medium according to the present invention uses burned livestock dung ash as the only inorganic source as described above, it does not contain fungi or bacteria due to its excellent composition.

The cost of the mineral source of the culture medium according to the present invention is the same as that of the conventional mineral source calculated from the chemical product price of inorganic salts. That fin in culture medium. It is extremely economical.

As described above, by using calcined animal dung ash as an inorganic source for the culture medium, culture costs can be reduced, and moreover, it is possible to effectively utilize calcined animal dung ash, which is a product of livestock waste that has no conventional use. Because you can open up! It is extremely effective in business.

Examples of the present invention are shown below.

Example t The following medium 1oo+#l/in a 50Owt Erlenmeyer flask
The mixture was placed in bales and evaporated at 110°C for 15 minutes.

Medium composition, yo! □ (# lll[) glucose 40 lll 40 lll vep
Ton 55 Jll dung burning station 1
〇 -KH2PO45 Mg5O47H20-2 M*80a, 6H20α006 and Bacillus porinxa A271 (
IFO14146) and cultured at 28°C in a rotary shaker. The production status of the viscous polysaccharide as described above can be investigated from the change in the viscosity of the culture solution over time. The results are shown in Table-2.

As shown in Table 2, there were differences in polysaccharide production by Bacillus polykina A271, production rate, production amount, and Fi between the four media.

Example 2 The following culture medium 50- was poured into a 500 d shoulder shaker flask and sterilized by heating at 110"C for 10 minutes.

Glucose 101/l 10t/l Begton 44 1111! Is Weihai 2 -KH2PO4
-1 Mg804.7H20-a 5 F@8047H200,01 Mm8044H20 [101 CaCO & -2 Naia Ken- 200 st/l 200 at/
1 biotin 5 iaf/l 5 sf/IP
R” 20 7.0 Brevibacterium 2ct 7 hermene cultured in advance on this / A
(Connect ATCC15B691 1a L.

Shaking culture was performed at 315°C for 40 hours.

As a result of the culture, the growth of Soda (indicated by 0, D) and the production amount of L-glutanic acid (indicated by t/dl) were as follows in medium 3.
α46 and 4.83 respectively. In medium 4, α was 49 and 4.86, respectively.Medium 3#i, in which the AM calcined ash according to the present invention was used as the sole mineral source, had no negative effects compared to the control.

Example & Donate 100 wd of the following culture medium in a shoulder-mounted shaker flask.
Dispense and sterilize with soda for 120 minutes.

11H2804 and adjusted with t'i NaOH, and inoculated with Penicillium frisogera^ (IFO4626).
Incubate at 25°C for 48 hours. The penicillin produced in the culture solutions of both was tested using the nine-cup method according to the penicillin assay method, and no significant difference #i was observed between the two.

Example 4 100 d of the following culture medium was dispensed into a 500-capacity shoulder shaker flask and sterilized at 110° C. for 15 minutes.

Liquid paraffin 20 t/l 20 t
/1NH4NOs 4 4 Chicken manure burnt ash 1011-K)12PO45 MgSO47H20-α4 Mail [LICaC12,2
H20-α1 Zys804.7H20-α7 MmSO46H20-α7 F@8047H20-α15 Cu804.5H20-α04 Ding W@11 20 al
α1PH&5 5.5 Qin 40 wt of 2NHvBOa was added in advance and boiled for 5 minutes. Candida intermedia (IFO0761) was inoculated thereto and cultured with shaking at 30°C for 72 hours. This culture 1
[Take 10m in a graduated test tube.

Centrifugation was performed at 35 ooxf for 10 minutes, and the amount of phyllodes was measured from the sedimentation volume. There was no significant difference in the amount of M medium.

Example 5 The following culture medium 10011 was placed in a 500 d shoulder shaker flask.
/ Dispense and heat at 110°C for 10 minutes! 11 Add 1 NHCl 25- in advance and boil for 5 minutes.
02) was harvested and cultured with shaking at 25°C for 7 days. Cultured fireflies are collected by filtration and dried.

The dry bacterial weight of 100 kg of culture medium was determined. The dry bacterial weight (f/dl) in medium 9 and medium 10 is
It was α524 and (LSI2) and there was almost no difference between the two.

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Claims (1)

[Claims] Culture medium containing burned livestock dung ash