JPS58108462A - Flow injection analysis system by pulse flow - Google Patents

Abstract

PURPOSE:To prevent the lowering of sensitivity and waste of carriers with limited diffusion of a sample zone due to unnecessary transfer thereof by halting or retaining the sample zone in a reaction pipe during the reaction with a structure of feeding carriers intermittently into a conduit. CONSTITUTION:An intermittent pump 8 sends a sufficient amount of carriers into a conduit 3 to allow feeding of all of a sample in a sample measuring tube 5 into a reaction pipe 6 and then, stops in preparation of the subsequent operation. Consequently, the sample is halted and retained in the reaction pipe 6, a condition under which a specified time elapses. Then, the intermittent pump 8 starts to work to discharge a reactant produced in the reaction pipe 6 passing through a detector 7 and stops. This permits the proceeding of the reaction halting the sample, eliminating lowered concentration due to diffusion occuring during the movement thereof in a long passage as seen in the past. A highly sensitive detection of the reactant can be done.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a flow injection analysis system using a pulsed flow, and in particular, in a flow type automatic analyzer, a sample zone undergoing reaction is retained and held in a reaction pipe, and the sample is prevented from being transferred unnecessarily. The present invention relates to a flow injection analysis system using pulsed flow, which is suitable for reducing zone diffusion to prevent a decrease in sensitivity and for preventing wasteful consumption of carriers.

First, the prior art will be explained using FIG. 1 and FIG. 2. FIG. 1 is an explanatory diagram of the configuration of a basic flow injection analysis system using continuous flow. In FIG. 1, 1 is a carrier vessel whose contents are conveyed in a continuous steady stream by a pump 2 through a conduit 3, a sampling pulp 4 and a reaction vibro to a detector 7. Reference numeral 5 denotes a sample measuring tube, in which the sample held in the sample measuring tube 5 is introduced into the carrier by operation of the sampling tube 4 (if the carrier is not the reaction liquid, the reaction liquid is also introduced at the same time). The anti-vibro reacts while passing through the vibro 5 and is transferred to the detector 7. By the way, since chemical reactions generally require a certain amount of time, anti-vibro
(react sample zone that continues to move during that time) (Eve 6
The length must be sufficient to hold it in the container (e.g. 2-10 m).

FIG. 2 is a diagram showing changes in the concentration distribution of the sample during that period. The crow in FIG. 2 is the concentration distribution immediately after introduction, b is the concentration distribution near the center of the reaction vibro, and the concentration distribution in the tube bundle of the C#'i reaction vibro. in this way,
Since the sample is subjected to diffusion during movement, the maximum concentration in the tube bundle is significantly reduced, which inevitably results in a decrease in detection sensitivity. Additionally, since Φyaya is flowing continuously during that time, carriers are wasted.

The present invention has been made in view of the above, and an object of the present invention is to provide a flow injection analysis system using a pulsed flow that can prevent a decrease in sensitivity and wasteful consumption of carriers.

A key feature of the present invention is that the feeding means for feeding carrier fluid or reaction fluid as a carrier into the conduit is configured to intermittently feed the carrier into the conduit.

The present invention will be described below with reference to FIG. 3, nine embodiments, and FIGS. 4 and 5.
This will be explained in detail using figures.

FIG. 3 is a configuration explanatory diagram showing an embodiment of the flow injection analysis system using pulsed flow of the present invention.
The same parts as in FIG. 1 are indicated by the same reference numerals, and the explanation will be omitted here. In FIG. 3, the pump 2 of FIG. 1 is replaced by an intermittent pump 8 that intermittently pumps the carrier (carrier liquid or reaction liquid) in the carrier container 1 into the conduit 3. Moreover, the intermittent pump a moves the sample in the sample measuring tube 5 to the reaction 7.
Once a sufficient amount of carrier is fed into the conduit 3, the carrier is stopped and ready for the next step. As a result, the sample is stopped and held in the reaction tube 6, and after a predetermined period of time in this state, the intermittent pump 8 starts operating again, and the reaction products generated in the reaction tube 6 are transferred to the detector 7. After passing through, it is discharged and then stopped.

The operation of these intermittent bobbins 8 is controlled by a control device (not shown).

According to the embodiment of the present invention described above, reaction no.
It only needs to be long enough to stop and hold the sample zone during the reaction, but since the reaction progresses while the sample is stopped, it will not occur while moving through a long flow path like in the past. There is no decrease in concentration due to diffusion, making it possible to detect reaction products with high sensitivity.

FIG. 4 is a diagram showing changes in the concentration distribution of the sample in the case of FIG. 3, where d is the concentration distribution immediately after the sample is introduced and the concentration distribution when it passes through the ELJ reaction ratio detector 7. Comparing this with C in FIG. 2, it can be seen that the decrease in density is extremely small.

FIG. 5 is an explanatory view showing the state of liquid feeding of the carrier according to the present invention.

Note that if the carrier is a reaction liquid, only the sample is added using the sampling pulp 4, but if the carrier is a carrier liquid that does not contain the reaction liquid, the reaction liquid is also added at the same time as the sample. This is the same as before. Furthermore, it goes without saying that the carrier may be a gas.

As explained above, according to the present invention, the sample zone undergoing reaction can be retained and retained in the reaction pipe.
This has the effect of reducing the spread of the sample zone due to unnecessary transport, thereby preventing a decrease in sensitivity and unnecessary consumption of carriers.

[Brief explanation of the drawing]

Figure 1 is an explanatory diagram of the configuration of a conventional flow injection analysis system using continuous flow, Figure 2 is a diagram showing changes in the concentration distribution of the sample in the case of Figure 1, and Figure 3 is a diagram showing the pulse flow of the present invention. A configuration explanatory diagram showing an example of a flow injection analysis system by
FIG. 5 is a diagram showing the change in the concentration of the sample in the case shown in FIG. 5, and FIG. 1...Carrier container, 3...Conduit, 4...Sampling pulp, 5...Sample measuring tube, 6...Reaction)z
Eve, 7th... /Z Fig. 2 Fig. 3 Sakafu lma4a's facial expression direction 411 No. 5z Gin rt+ -

Claims (1)

[Claims] 1. A feeding means for feeding a carrier fluid or a reaction fluid into the conduit as a carrier, and a sampling valve for adding a predetermined amount of sample or a predetermined amount of sample and reaction fluid to the flow of fluid in the conduit. A flow injection analysis system comprising: a reaction pipe for mixing and reacting the sample and a reaction fluid; and a detection means for measuring the amount of chemical or physical change that occurs due to the reaction within the reaction pipe. . A flow injection analysis system using pulsed flow, characterized in that the feeding means is configured to continuously feed the carrier into the conduit.