JPS5786299A - Plasmid and its preparation - Google Patents
Plasmid and its preparationInfo
- Publication number
- JPS5786299A JPS5786299A JP55163054A JP16305480A JPS5786299A JP S5786299 A JPS5786299 A JP S5786299A JP 55163054 A JP55163054 A JP 55163054A JP 16305480 A JP16305480 A JP 16305480A JP S5786299 A JPS5786299 A JP S5786299A
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- bacillus subtilis
- streptococcus faecalis
- ptp62
- cultivated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013612 plasmid Substances 0.000 title abstract 7
- 241000194032 Enterococcus faecalis Species 0.000 abstract 3
- 244000063299 Bacillus subtilis Species 0.000 abstract 2
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 2
- 241000276408 Bacillus subtilis subsp. subtilis str. 168 Species 0.000 abstract 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 abstract 1
- 229930024421 Adenine Natural products 0.000 abstract 1
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 102000016943 Muramidase Human genes 0.000 abstract 1
- 108010014251 Muramidase Proteins 0.000 abstract 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 abstract 1
- 229960000643 adenine Drugs 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 238000000432 density-gradient centrifugation Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 229960000274 lysozyme Drugs 0.000 abstract 1
- 235000010335 lysozyme Nutrition 0.000 abstract 1
- 239000004325 lysozyme Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 230000002463 transducing effect Effects 0.000 abstract 1
- 230000026683 transduction Effects 0.000 abstract 1
- 238000010361 transduction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55163054A JPS5786299A (en) | 1980-11-19 | 1980-11-19 | Plasmid and its preparation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55163054A JPS5786299A (en) | 1980-11-19 | 1980-11-19 | Plasmid and its preparation |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5786299A true JPS5786299A (en) | 1982-05-29 |
JPH0139751B2 JPH0139751B2 (enrdf_load_stackoverflow) | 1989-08-23 |
Family
ID=15766292
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55163054A Granted JPS5786299A (en) | 1980-11-19 | 1980-11-19 | Plasmid and its preparation |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5786299A (enrdf_load_stackoverflow) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60217898A (ja) * | 1984-03-28 | 1985-10-31 | シタス コーポレイシヨン | 異種タンパス質の製造方法 |
JPH06319534A (ja) * | 1983-06-24 | 1994-11-22 | Genentech Inc | サブチリシン突然変異体の製造に有用な桿菌 |
-
1980
- 1980-11-19 JP JP55163054A patent/JPS5786299A/ja active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06319534A (ja) * | 1983-06-24 | 1994-11-22 | Genentech Inc | サブチリシン突然変異体の製造に有用な桿菌 |
JPS60217898A (ja) * | 1984-03-28 | 1985-10-31 | シタス コーポレイシヨン | 異種タンパス質の製造方法 |
Also Published As
Publication number | Publication date |
---|---|
JPH0139751B2 (enrdf_load_stackoverflow) | 1989-08-23 |
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