JPS5786299A - Plasmid and its preparation - Google Patents

Plasmid and its preparation

Info

Publication number
JPS5786299A
JPS5786299A JP55163054A JP16305480A JPS5786299A JP S5786299 A JPS5786299 A JP S5786299A JP 55163054 A JP55163054 A JP 55163054A JP 16305480 A JP16305480 A JP 16305480A JP S5786299 A JPS5786299 A JP S5786299A
Authority
JP
Japan
Prior art keywords
plasmid
bacillus subtilis
streptococcus faecalis
ptp62
cultivated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55163054A
Other languages
Japanese (ja)
Other versions
JPH0139751B2 (en
Inventor
Megumi Kono
Mitsunori Sasatsu
Hajime Hamashima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daiichi Pharmaceutical Co Ltd
Original Assignee
Daiichi Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Pharmaceutical Co Ltd filed Critical Daiichi Pharmaceutical Co Ltd
Priority to JP55163054A priority Critical patent/JPS5786299A/en
Publication of JPS5786299A publication Critical patent/JPS5786299A/en
Publication of JPH0139751B2 publication Critical patent/JPH0139751B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Abstract

PURPOSE:To obtain a novel plasmid usable as a vector, by separating a plasmid from Streptococcus faecalis, introducing (transducing) the separated plasmid into Bacillus substilis, and multiplying the Bacillus subtilis. CONSTITUTION:Streptococcus faecalis (FERM-P No.5772) is cultivated by the conventional method, and the collected microbial cells are subjected to the lysis with a lysozyme and then separated by the density gradient centrifugation method of CsCl to give the aimed plasmid (PTP62) having a molecular weight of 9.1X10<6> daltons and properties shown in the table. The resultant plasmid is then introduced (transduced) into a microorganism, e.g. a strain of Bacillus subtilis 168, requiring adenine, and deficient in a restriction enzyme and modifying enzyme, and the Bacillus subtilis is then cultivated to separate the plasmid PTP62 from the multiplied microorganisms in the same way as in the separation from the Streptococcus faecalis and introduction (transduction) into the Bacillus subtilis 168.
JP55163054A 1980-11-19 1980-11-19 Plasmid and its preparation Granted JPS5786299A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55163054A JPS5786299A (en) 1980-11-19 1980-11-19 Plasmid and its preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55163054A JPS5786299A (en) 1980-11-19 1980-11-19 Plasmid and its preparation

Publications (2)

Publication Number Publication Date
JPS5786299A true JPS5786299A (en) 1982-05-29
JPH0139751B2 JPH0139751B2 (en) 1989-08-23

Family

ID=15766292

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55163054A Granted JPS5786299A (en) 1980-11-19 1980-11-19 Plasmid and its preparation

Country Status (1)

Country Link
JP (1) JPS5786299A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60217898A (en) * 1984-03-28 1985-10-31 シタス　コーポレイシヨン Production of heterogenic protein
JPH06319534A (en) * 1983-06-24 1994-11-22 Genentech Inc Corynebacterium useful in production of subtilisin mutant

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06319534A (en) * 1983-06-24 1994-11-22 Genentech Inc Corynebacterium useful in production of subtilisin mutant
JPS60217898A (en) * 1984-03-28 1985-10-31 シタス　コーポレイシヨン Production of heterogenic protein

Also Published As

Publication number Publication date
JPH0139751B2 (en) 1989-08-23

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