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TOHO YAKUHIN KOGYO KK
TOUHOU YAKUHIN KOGYO KK
Original Assignee
TOHO YAKUHIN KOGYO KK
TOUHOU YAKUHIN KOGYO KK
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Application filed by TOHO YAKUHIN KOGYO KK, TOUHOU YAKUHIN KOGYO KKfiledCriticalTOHO YAKUHIN KOGYO KK
Priority to JP15262580ApriorityCriticalpatent/JPS5774088A/en
Publication of JPS5774088ApublicationCriticalpatent/JPS5774088A/en
PURPOSE: Estrase obtained from internal organs of mammalian animals is simply and efficiently purified by utilizing its characteristic affinity to the residual group of glycine or alanine.
CONSTITUTION: Glycine or alanine is bonded through a spacer with a support such agarose or dextran and the resultant support is brought into contact with crude estrase obtained from internal organs of mammalian animals. Then, contaminating foreign proteins are eluted with a buffer solution free from sodium chloride, preferably a phosphate buffer solution of about 7.4pH, then, the estrase held on the support is eluted with a buffer solution containing less than 0.2M sodium chloride, preferably by the gradient method in which the concentration of sodium chloride gradually increases from 0 to 0.2M.
COPYRIGHT: (C)1982,JPO&Japio
JP15262580A1980-10-291980-10-29Purification of estrase
PendingJPS5774088A
(en)