ATE76082T1 - PURIFICATION METHOD FOR ANTIBODIES. - Google Patents

PURIFICATION METHOD FOR ANTIBODIES.

Info

Publication number
ATE76082T1
ATE76082T1 AT87308900T AT87308900T ATE76082T1 AT E76082 T1 ATE76082 T1 AT E76082T1 AT 87308900 T AT87308900 T AT 87308900T AT 87308900 T AT87308900 T AT 87308900T AT E76082 T1 ATE76082 T1 AT E76082T1
Authority
AT
Austria
Prior art keywords
antibodies
purification method
antibody
matrix
column
Prior art date
Application number
AT87308900T
Other languages
German (de)
Inventor
Gene R Nathans
Robert W Rosenstein
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Application granted granted Critical
Publication of ATE76082T1 publication Critical patent/ATE76082T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Control Of El Displays (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

I(g)G3 antibody is purified by affinity chromatography and collection of released antibody at a pH of 9.0 to 9.6. The purification is effected in a column containing both an affinity matrix and a desalting matrix, with the column being equilibrated to a pH of from 9.0 to 9.6. I(g)G3 antibody may be stored at pH 9.0 to 9.6.
AT87308900T 1987-10-05 1987-10-07 PURIFICATION METHOD FOR ANTIBODIES. ATE76082T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA000548559A CA1323567C (en) 1987-10-05 1987-10-05 Method for purification of antibodies
EP87308900A EP0310719B1 (en) 1987-10-05 1987-10-07 Method for purification of antibodies

Publications (1)

Publication Number Publication Date
ATE76082T1 true ATE76082T1 (en) 1992-05-15

Family

ID=25671537

Family Applications (1)

Application Number Title Priority Date Filing Date
AT87308900T ATE76082T1 (en) 1987-10-05 1987-10-07 PURIFICATION METHOD FOR ANTIBODIES.

Country Status (9)

Country Link
EP (1) EP0310719B1 (en)
JP (1) JPH0696598B2 (en)
AT (1) ATE76082T1 (en)
AU (1) AU600311B2 (en)
CA (1) CA1323567C (en)
DE (1) DE3779125D1 (en)
ES (1) ES2041690T3 (en)
FI (1) FI90082C (en)
GR (1) GR3004622T3 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5151504A (en) * 1989-11-17 1992-09-29 E. R. Squibb & Sons, Inc. Method for purification of monoclonal antibodies
DE4118912C1 (en) * 1991-06-08 1992-07-02 Biotest Pharma Gmbh, 6072 Dreieich, De
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
WO2016031932A1 (en) * 2014-08-27 2016-03-03 田辺三菱製薬株式会社 METHOD FOR PRODUCING PROTEIN HAVING Fc REGION BY ALKALI WASHING
MX2017011121A (en) * 2015-03-13 2017-11-28 Bristol Myers Squibb Co Use of alkaline washes during chromatography to remove impurities.

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES521371A0 (en) * 1982-04-12 1984-05-16 Hybritech Inc A PROCEDURE FOR THE PURIFICATION OF AN ANTIBODY.
JPS60228421A (en) * 1984-04-27 1985-11-13 Shionogi & Co Ltd Monoclonal anti-human-igg antibody and its preparation

Also Published As

Publication number Publication date
FI874431A (en) 1989-04-09
ES2041690T3 (en) 1993-12-01
DE3779125D1 (en) 1992-06-17
AU7955687A (en) 1989-04-13
FI90082B (en) 1993-09-15
GR3004622T3 (en) 1993-04-28
CA1323567C (en) 1993-10-26
EP0310719A1 (en) 1989-04-12
EP0310719B1 (en) 1992-05-13
FI874431A0 (en) 1987-10-08
AU600311B2 (en) 1990-08-09
JPH0696598B2 (en) 1994-11-30
FI90082C (en) 1993-12-27
JPH01135798A (en) 1989-05-29

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee