JPS5742854A - Latex sensitized with complement 1q - Google Patents

Latex sensitized with complement 1q

Info

Publication number
JPS5742854A
JPS5742854A JP55117034A JP11703480A JPS5742854A JP S5742854 A JPS5742854 A JP S5742854A JP 55117034 A JP55117034 A JP 55117034A JP 11703480 A JP11703480 A JP 11703480A JP S5742854 A JPS5742854 A JP S5742854A
Authority
JP
Japan
Prior art keywords
complement
latex
sensitized
solution
serum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55117034A
Other languages
Japanese (ja)
Inventor
Tetsuo Tomiyama
Taku Ogura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Priority to JP55117034A priority Critical patent/JPS5742854A/en
Publication of JPS5742854A publication Critical patent/JPS5742854A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • Rehabilitation Therapy (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PURPOSE:To obtain a latex sensitized with complement 1q, which is enabled to determined easily an immune complex (1C) by a microtitration process, by allowing to carry a complement 1q on the surface of a particle. CONSTITUTION:A serum such as a human serum is dialyzed against ethyleneglycol-bis(aminoethyl)-tetra acetic acid to obtain a purified complement 1q. Next, a synthetic high polymer latex (particle size of 0.01-10mu polystyrene latex is preferable) is diluted with a physicological salt solution and a buffer solution to 0.05-3% concentration of latex particles and the complement 1q is added to this solution, then allowed to contact at 0-4 deg.C for 3-24hrs to obtain the said sensitized latex.
JP55117034A 1980-08-27 1980-08-27 Latex sensitized with complement 1q Pending JPS5742854A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55117034A JPS5742854A (en) 1980-08-27 1980-08-27 Latex sensitized with complement 1q

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55117034A JPS5742854A (en) 1980-08-27 1980-08-27 Latex sensitized with complement 1q

Publications (1)

Publication Number Publication Date
JPS5742854A true JPS5742854A (en) 1982-03-10

Family

ID=14701798

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55117034A Pending JPS5742854A (en) 1980-08-27 1980-08-27 Latex sensitized with complement 1q

Country Status (1)

Country Link
JP (1) JPS5742854A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5126220A (en) * 1974-05-20 1976-03-04 Technicon Instr
JPS5530692A (en) * 1978-08-17 1980-03-04 Behringwerke Ag Method of immunological measuring

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5126220A (en) * 1974-05-20 1976-03-04 Technicon Instr
JPS5126221A (en) * 1974-05-20 1976-03-04 Technicon Instr
JPS5530692A (en) * 1978-08-17 1980-03-04 Behringwerke Ag Method of immunological measuring

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