JPS572697A - Simultaneous production of sterol with red dye chrysanthemin by tissual culture of euphorbia millii ch. des moulins - Google Patents

Simultaneous production of sterol with red dye chrysanthemin by tissual culture of euphorbia millii ch. des moulins

Info

Publication number
JPS572697A
JPS572697A JP7496880A JP7496880A JPS572697A JP S572697 A JPS572697 A JP S572697A JP 7496880 A JP7496880 A JP 7496880A JP 7496880 A JP7496880 A JP 7496880A JP S572697 A JPS572697 A JP S572697A
Authority
JP
Japan
Prior art keywords
substances
chrysanthemin
sterol
euphorbia
red dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP7496880A
Other languages
Japanese (ja)
Other versions
JPS6331200B2 (en
Inventor
Yoshikazu Yamamoto
Ryuzo Mizuguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Paint Co Ltd
Original Assignee
Nippon Paint Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Paint Co Ltd filed Critical Nippon Paint Co Ltd
Priority to JP7496880A priority Critical patent/JPS572697A/en
Publication of JPS572697A publication Critical patent/JPS572697A/en
Publication of JPS6331200B2 publication Critical patent/JPS6331200B2/ja
Granted legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: To collect a red dye chrysanthemin and sterol,by cultivating a callus derived from a plant tissue of Euphorbia Millii Ch. des Moulins in a culture medium containing an α-naphthaleneacetic acid, sucrose, etc. under irradiation with light at a specific lux value or more.
CONSTITUTION: Leaves of Euphorbia Millii Ch. des Moulins which is a plant of the genus Euphorbia are sterilized, cut to small pieces, and cultivated to form a callus. The resultant callus is then cultivated in a culture medium containing an α-naphthaleneacetic acid in a concentration of 10-4W10-7M, sucrose in an amount of 1W10wt%, organic substances in a very small amount, various natural extracted substances, etc. in the well-known inorganic synthetic agar culture medium. The cultivation is carried out under irradiation with light at 100 luxes or more. The aimed substances are obtained as follows: Sterol is collected by immersing the cultivated cells in an acid, water or alcohol, extracting the aimed substances, removing the solid substances, concentrating the residue, and extracting the residue with an ether. The remaining solution is then dried under reduced pressure to afford a red dye chrysanthemin of the formula.
COPYRIGHT: (C)1982,JPO&Japio
JP7496880A 1980-06-05 1980-06-05 Simultaneous production of sterol with red dye chrysanthemin by tissual culture of euphorbia millii ch. des moulins Granted JPS572697A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7496880A JPS572697A (en) 1980-06-05 1980-06-05 Simultaneous production of sterol with red dye chrysanthemin by tissual culture of euphorbia millii ch. des moulins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7496880A JPS572697A (en) 1980-06-05 1980-06-05 Simultaneous production of sterol with red dye chrysanthemin by tissual culture of euphorbia millii ch. des moulins

Publications (2)

Publication Number Publication Date
JPS572697A true JPS572697A (en) 1982-01-08
JPS6331200B2 JPS6331200B2 (en) 1988-06-22

Family

ID=13562595

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7496880A Granted JPS572697A (en) 1980-06-05 1980-06-05 Simultaneous production of sterol with red dye chrysanthemin by tissual culture of euphorbia millii ch. des moulins

Country Status (1)

Country Link
JP (1) JPS572697A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0479459A2 (en) * 1990-09-17 1992-04-08 Nippon Paint Co., Ltd. Production of quercetin glucuronide and cultured cells containing the same
CN103232513A (en) * 2013-05-09 2013-08-07 南京中医药大学 Method for preparing tirucallol

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02112796U (en) * 1989-02-21 1990-09-10

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0479459A2 (en) * 1990-09-17 1992-04-08 Nippon Paint Co., Ltd. Production of quercetin glucuronide and cultured cells containing the same
CN103232513A (en) * 2013-05-09 2013-08-07 南京中医药大学 Method for preparing tirucallol
CN103232513B (en) * 2013-05-09 2015-06-10 南京中医药大学 Method for preparing tirucallol

Also Published As

Publication number Publication date
JPS6331200B2 (en) 1988-06-22

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