JPS5719664A - Measuring method for human helper t cell function - Google Patents

Measuring method for human helper t cell function

Info

Publication number
JPS5719664A
JPS5719664A JP9437080A JP9437080A JPS5719664A JP S5719664 A JPS5719664 A JP S5719664A JP 9437080 A JP9437080 A JP 9437080A JP 9437080 A JP9437080 A JP 9437080A JP S5719664 A JPS5719664 A JP S5719664A
Authority
JP
Japan
Prior art keywords
cell
normal person
isolated
red blood
function
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP9437080A
Other languages
Japanese (ja)
Inventor
Tsuneo Kuramochi
Shoji Maruyama
Toshisada Ishido
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SOGO SEIBUTSU IGAKU KENKYUSHO
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Original Assignee
SOGO SEIBUTSU IGAKU KENKYUSHO
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SOGO SEIBUTSU IGAKU KENKYUSHO, SOGO SEIBUTSU IGAKU KENKYUSHO KK filed Critical SOGO SEIBUTSU IGAKU KENKYUSHO
Priority to JP9437080A priority Critical patent/JPS5719664A/en
Publication of JPS5719664A publication Critical patent/JPS5719664A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To measure easily a helper T cell function in vitro, by culturing a T cell isolated from bright red blood of a patient along with a B cell isolated from bright red blood of a normal person under a specific condition and by using <3>H-labelled thymidine. CONSTITUTION:A helper T cell (having a function of helping antibody production) is isolated from bright red blood of a patient and cultured in the presence of a Tm cell having a suppressor function (suppressing antibody production) controlled by mitomycin treatment, a B cell and B cell mitogen (PWM promoting mitosis) isolated from bright red blood of a normal person and <3>H-labelled thymidine. On the other hand, a Tm cell of a normal person is cultured in the same way by adding the same B cell and PWM of a normal person and the same <3>H-labelled thymidine. An amount of DNA synthesized in a process where the B cell differentiates and proliferates is measured by determining, with a toluene scintillator, the <3>H-labelled substance incorporated in the B cell collected after the proliferation. An abnormal part of a patient suffering from immune disease is diagnozed bosed on differences in a helper function between the patient and the normal person by comparing their radioactivities, to thereby facilitate medical treatment.
JP9437080A 1980-07-10 1980-07-10 Measuring method for human helper t cell function Pending JPS5719664A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9437080A JPS5719664A (en) 1980-07-10 1980-07-10 Measuring method for human helper t cell function

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9437080A JPS5719664A (en) 1980-07-10 1980-07-10 Measuring method for human helper t cell function

Publications (1)

Publication Number Publication Date
JPS5719664A true JPS5719664A (en) 1982-02-01

Family

ID=14108422

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9437080A Pending JPS5719664A (en) 1980-07-10 1980-07-10 Measuring method for human helper t cell function

Country Status (1)

Country Link
JP (1) JPS5719664A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59193360A (en) * 1983-02-10 1984-11-01 ベスター・インコーポレイテツド Method of marking phagocyte

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59193360A (en) * 1983-02-10 1984-11-01 ベスター・インコーポレイテツド Method of marking phagocyte
JPH0354307B2 (en) * 1983-02-10 1991-08-19

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