JPS57182145A - Aminoacid fluorometry - Google Patents
Aminoacid fluorometryInfo
- Publication number
- JPS57182145A JPS57182145A JP6620681A JP6620681A JPS57182145A JP S57182145 A JPS57182145 A JP S57182145A JP 6620681 A JP6620681 A JP 6620681A JP 6620681 A JP6620681 A JP 6620681A JP S57182145 A JPS57182145 A JP S57182145A
- Authority
- JP
- Japan
- Prior art keywords
- specimen
- react
- buffer solution
- existence
- making
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
PURPOSE:To suppress obstruction by a moving phase and ammonia and at the same time raise sensitivity to cyclic aminoacids by making a specimen react with an oxidizing agent under the existence of the buffer solution of a metallic salt and then making the specimen react with a fluorescent reagent. CONSTITUTION:After cleaning liquid 26 and a first moving phase 23 are made to flow from a liquid conveying section 12, specimen is poured from a specimen supply section 13, and further, a first, second and third moving phases 23-25 are mae to flow through a play column 20. A specimen is separated as needed from a separation column 14, and next it is made to react for more than 5sec in a first reaction coil 16 with an oxidizing reagent 21 that contains chlorous acid sodium solution under the existence of a buffer solution that consists of sodium bicarbonate of pH 9.5-9.9 and sodium carbonate. Next, the specimen is made to react in a second reaction coil 18 for over 30sec in a buffer solution of pH 9.5-9.9 with a fluorescent reagent 22 that has a non-ion surface active agent such as Brij 35 in high concentration.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6620681A JPS57182145A (en) | 1981-05-02 | 1981-05-02 | Aminoacid fluorometry |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6620681A JPS57182145A (en) | 1981-05-02 | 1981-05-02 | Aminoacid fluorometry |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57182145A true JPS57182145A (en) | 1982-11-09 |
Family
ID=13309124
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6620681A Pending JPS57182145A (en) | 1981-05-02 | 1981-05-02 | Aminoacid fluorometry |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57182145A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60253973A (en) * | 1984-05-31 | 1985-12-14 | Japan Spectroscopic Co | Method and instrument for analyzing amine |
US4865994A (en) * | 1985-05-17 | 1989-09-12 | Seiko Instruments & Electronics Ltd. | Detection method for amino acid derivatives |
JP2013180289A (en) * | 2012-03-05 | 2013-09-12 | Sumitomo Metal Mining Co Ltd | Flotation machine |
-
1981
- 1981-05-02 JP JP6620681A patent/JPS57182145A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60253973A (en) * | 1984-05-31 | 1985-12-14 | Japan Spectroscopic Co | Method and instrument for analyzing amine |
US4865994A (en) * | 1985-05-17 | 1989-09-12 | Seiko Instruments & Electronics Ltd. | Detection method for amino acid derivatives |
JP2013180289A (en) * | 2012-03-05 | 2013-09-12 | Sumitomo Metal Mining Co Ltd | Flotation machine |
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