JPS57181694A - Cultivation of fibroblast - Google Patents

Cultivation of fibroblast

Info

Publication number
JPS57181694A
JPS57181694A JP56066493A JP6649381A JPS57181694A JP S57181694 A JPS57181694 A JP S57181694A JP 56066493 A JP56066493 A JP 56066493A JP 6649381 A JP6649381 A JP 6649381A JP S57181694 A JPS57181694 A JP S57181694A
Authority
JP
Japan
Prior art keywords
medium
cultivation
cells
serum
gas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56066493A
Other languages
Japanese (ja)
Inventor
Isao Yamane
Mikio Suga
Hiroyoshi Hoshi
Yoshiki Minamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP56066493A priority Critical patent/JPS57181694A/en
Publication of JPS57181694A publication Critical patent/JPS57181694A/en
Pending legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: To culture the titled cells originated from mammalian animals, by keeping the oxygen concentration in the gas contacting with the medium at a definite level, adding the titled cells to the medium, and adding the serum albumin separated from the serum of mammalian animal to the medium.
CONSTITUTION: A conventional cell propiferation medium is used as the base medium, and all components except serum albumin are dissolved in water at definite concentrations followed by disinfection and filtration. Fibroblast cells of a mammalian animal, e.g. man, cattle, horse, mouse, pig, etc. are added to the above medium, and then serum aibumin originated from a mammalian animal is added thereto. The gas passing through the medium during cultivation has an oxygen content of 3W12vol%, and contains carbon dioxide gas, nitrogen gas, etc. as other components. The cultivation is carried out by conventional method, and the useful substance such as proliferated cells or interferon, etc. is separated from the cultivation liquid after the cultivation.
COPYRIGHT: (C)1982,JPO&Japio
JP56066493A 1981-05-01 1981-05-01 Cultivation of fibroblast Pending JPS57181694A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56066493A JPS57181694A (en) 1981-05-01 1981-05-01 Cultivation of fibroblast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56066493A JPS57181694A (en) 1981-05-01 1981-05-01 Cultivation of fibroblast

Publications (1)

Publication Number Publication Date
JPS57181694A true JPS57181694A (en) 1982-11-09

Family

ID=13317380

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56066493A Pending JPS57181694A (en) 1981-05-01 1981-05-01 Cultivation of fibroblast

Country Status (1)

Country Link
JP (1) JPS57181694A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105219699A (en) * 2015-10-15 2016-01-06 南京三生生物技术有限公司 A kind of low serum protein-free medium being suitable for BHK21 Growth of Cells and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105219699A (en) * 2015-10-15 2016-01-06 南京三生生物技术有限公司 A kind of low serum protein-free medium being suitable for BHK21 Growth of Cells and preparation method thereof

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