JPS57181694A - Cultivation of fibroblast - Google Patents
Cultivation of fibroblastInfo
- Publication number
- JPS57181694A JPS57181694A JP56066493A JP6649381A JPS57181694A JP S57181694 A JPS57181694 A JP S57181694A JP 56066493 A JP56066493 A JP 56066493A JP 6649381 A JP6649381 A JP 6649381A JP S57181694 A JPS57181694 A JP S57181694A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- cultivation
- cells
- serum
- gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: To culture the titled cells originated from mammalian animals, by keeping the oxygen concentration in the gas contacting with the medium at a definite level, adding the titled cells to the medium, and adding the serum albumin separated from the serum of mammalian animal to the medium.
CONSTITUTION: A conventional cell propiferation medium is used as the base medium, and all components except serum albumin are dissolved in water at definite concentrations followed by disinfection and filtration. Fibroblast cells of a mammalian animal, e.g. man, cattle, horse, mouse, pig, etc. are added to the above medium, and then serum aibumin originated from a mammalian animal is added thereto. The gas passing through the medium during cultivation has an oxygen content of 3W12vol%, and contains carbon dioxide gas, nitrogen gas, etc. as other components. The cultivation is carried out by conventional method, and the useful substance such as proliferated cells or interferon, etc. is separated from the cultivation liquid after the cultivation.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56066493A JPS57181694A (en) | 1981-05-01 | 1981-05-01 | Cultivation of fibroblast |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56066493A JPS57181694A (en) | 1981-05-01 | 1981-05-01 | Cultivation of fibroblast |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57181694A true JPS57181694A (en) | 1982-11-09 |
Family
ID=13317380
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56066493A Pending JPS57181694A (en) | 1981-05-01 | 1981-05-01 | Cultivation of fibroblast |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57181694A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105219699A (en) * | 2015-10-15 | 2016-01-06 | 南京三生生物技术有限公司 | A kind of low serum protein-free medium being suitable for BHK21 Growth of Cells and preparation method thereof |
-
1981
- 1981-05-01 JP JP56066493A patent/JPS57181694A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105219699A (en) * | 2015-10-15 | 2016-01-06 | 南京三生生物技术有限公司 | A kind of low serum protein-free medium being suitable for BHK21 Growth of Cells and preparation method thereof |
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