JPS57130920A - Antitumor agent and its preparation - Google Patents

Abstract

PURPOSE: An antitumor agent having extremely inhibiting action on tumor propagation, comprising an extract as a main component obtained from Marsdenia tenacissima (Rox b) Wight et Arm., soluble in a 1ower alcohol and a chlorinated hydrocarbon (other than CCl₄), insoluble in an aliphatic hydrocarbon.

CONSTITUTION: Marsdenia tenacissima (Rox b) Wight et Arm, a plant belonging to the family Asclepiadaceae, is cut into pices to give a raw material crude drug, to which a lower alcohol (prefefably methanol) on an amount of 2W3 times (V/W) as much as that of the crude drug, is added it is allowed to stand at room temperature for several hoursWtens of hours, extracted, and separated to give an extracted solution. The residue is subjected to the similar extraction and filtration operations repeatedly, and all of the ixtracted solutions are gathered and concentrated to dryness under reduced pressure. Tothe prepared extract a chlorinated hydrocarbon (preferably chloroform) except carbon tetrachloride in an amount of 2W6 times (V/W) that of the extract is added, the extract is shaden vigorusly and extracted, to give an extracted solution, which is concentrated under reduced prssure and evaporated to dryness. The extract is dissolved in the minimum amount of the abive-mentioned chlorinated hydrocarbon, an aliphatic hydrocarbon in an amount of 2W4 times (V/W) that of the resulted solution is added to the solution, and an insolulbe part is removed to give the desired extract.

COPYRIGHT: (C)1982,JPO&Japio