JPS57100340A - Measuring apparatus of electrophoresis of cell - Google Patents

Measuring apparatus of electrophoresis of cell

Info

Publication number
JPS57100340A
JPS57100340A JP55177641A JP17764180A JPS57100340A JP S57100340 A JPS57100340 A JP S57100340A JP 55177641 A JP55177641 A JP 55177641A JP 17764180 A JP17764180 A JP 17764180A JP S57100340 A JPS57100340 A JP S57100340A
Authority
JP
Japan
Prior art keywords
cell
electrophoresis
slit
electric field
measured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55177641A
Other languages
Japanese (ja)
Other versions
JPS6225989B2 (en
Inventor
Hidehiko Fujii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Shimazu Seisakusho KK
Original Assignee
Shimadzu Corp
Shimazu Seisakusho KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp, Shimazu Seisakusho KK filed Critical Shimadzu Corp
Priority to JP55177641A priority Critical patent/JPS57100340A/en
Publication of JPS57100340A publication Critical patent/JPS57100340A/en
Publication of JPS6225989B2 publication Critical patent/JPS6225989B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To achieve long-time pursuit, by applying an alternating current electric field to a floating fluid of cell and executing reciprocating motion of the cell by electrophoresis. CONSTITUTION:Parallel plate electrodes 2, 3 are arranged in opposition to each other in a square bath 1 having a larger dimension in a depth direction than the width in the left and right direction. A buffer solution 4 containing the same volume as blood is filled in the bath 1 and the floating liquid of a living body cell is injected from a nozzle 5. When the square wave voltage inverting the polarity at intervals of a constant time is applied between the electrodes 2, 3, the cell injected from the nozzle 5 is settled down while executing reciprocating motion in left and right by receiving the action of an electric field. The motion of this cell is observed photoelectrically through a slit 6. The velocity of electrophoresis is measured by a time T receiving by a photodetector P provided on the back of the slit 6 and a slit width L and the mean velocity is measured by the height of the slit. Also, the degree of electrophoresis is measured by the distance between the electrodes and the intensity of the electric field.
JP55177641A 1980-12-15 1980-12-15 Measuring apparatus of electrophoresis of cell Granted JPS57100340A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55177641A JPS57100340A (en) 1980-12-15 1980-12-15 Measuring apparatus of electrophoresis of cell

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55177641A JPS57100340A (en) 1980-12-15 1980-12-15 Measuring apparatus of electrophoresis of cell

Publications (2)

Publication Number Publication Date
JPS57100340A true JPS57100340A (en) 1982-06-22
JPS6225989B2 JPS6225989B2 (en) 1987-06-05

Family

ID=16034536

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55177641A Granted JPS57100340A (en) 1980-12-15 1980-12-15 Measuring apparatus of electrophoresis of cell

Country Status (1)

Country Link
JP (1) JPS57100340A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62285062A (en) * 1986-06-04 1987-12-10 Agency Of Ind Science & Technol Immuno assay apparatus
FR2752751A1 (en) * 1996-09-02 1998-03-06 Home Alexandre Purification of organic material by electrophoresis
JP2007232400A (en) * 2006-02-27 2007-09-13 National Institute Of Advanced Industrial & Technology Affinity analyzer for biomolecule, and method for analyzing affinity between biomolecules using analyzer
JP2017526939A (en) * 2014-07-07 2017-09-14 ロゴス バイオシステムズ， インコーポレイテッド Tissue clearing device using electrophoresis

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100344767C (en) * 2005-12-01 2007-10-24 中国科学院山西煤炭化学研究所 Rich alpha-linolenic acid contained polyene phosphatidylcholine and its production method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62285062A (en) * 1986-06-04 1987-12-10 Agency Of Ind Science & Technol Immuno assay apparatus
JPH0529065B2 (en) * 1986-06-04 1993-04-28 Kogyo Gijutsuin
FR2752751A1 (en) * 1996-09-02 1998-03-06 Home Alexandre Purification of organic material by electrophoresis
JP2007232400A (en) * 2006-02-27 2007-09-13 National Institute Of Advanced Industrial & Technology Affinity analyzer for biomolecule, and method for analyzing affinity between biomolecules using analyzer
JP4684915B2 (en) * 2006-02-27 2011-05-18 独立行政法人産業技術総合研究所 Biomolecule affinity analyzer and method for analyzing affinity between biomolecules using the device
JP2017526939A (en) * 2014-07-07 2017-09-14 ロゴス バイオシステムズ， インコーポレイテッド Tissue clearing device using electrophoresis

Also Published As

Publication number Publication date
JPS6225989B2 (en) 1987-06-05

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