JPS5696694A - Separation of myeloperoxidase from human nyelogenic leukocite - Google Patents

Separation of myeloperoxidase from human nyelogenic leukocite

Info

Publication number
JPS5696694A
JPS5696694A JP17210979A JP17210979A JPS5696694A JP S5696694 A JPS5696694 A JP S5696694A JP 17210979 A JP17210979 A JP 17210979A JP 17210979 A JP17210979 A JP 17210979A JP S5696694 A JPS5696694 A JP S5696694A
Authority
JP
Japan
Prior art keywords
myeloperoxidase
supernatant
human
suspension
leukocites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17210979A
Other languages
Japanese (ja)
Inventor
Takashi Kobayashi
Eiichi Hasegawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Priority to JP17210979A priority Critical patent/JPS5696694A/en
Priority to SE8005679A priority patent/SE448882B/en
Priority to US06/179,783 priority patent/US4306025A/en
Priority to DE3031990A priority patent/DE3031990C2/en
Priority to FR8018581A priority patent/FR2472609A1/en
Priority to AT0436780A priority patent/AT370997B/en
Priority to IN758/CAL/81A priority patent/IN153488B/en
Publication of JPS5696694A publication Critical patent/JPS5696694A/en
Priority to US06/297,418 priority patent/US4379141A/en
Priority to GB8233109A priority patent/GB2108387B/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: At least one selected from manganese, manganese salts, and protamine sulfate is added to and mixed with an aqueous suspension of crushed human myelogenic leukocites and the supernatant is treated to separate and recover human- originating myeloperoxidase of high quality in high yield.
CONSTITUTION: An aqueous suspension of human myelogenic leukocites is treated with a homogenizer at 0°C for 5min to prepare an aqueous suspension of crushed leukocites, which is subjected to following treatments. The suspension is centrifuged into the precipitate and the supernatant and the yellowish green supernatant is recovered as a fraction containing myeloperoxidase. The fraction is suspended in a buffer solution of pH6W8.0 containing potassium phosphate and homogenized. Then, at least one selected from Mn, Mn salts and protamine sulfate is added to the homogenized suspension. The resultant suspension is centrifuged again to separate the myeloperoxidase fraction, i.e., the supernatant, which is subjected to cation exchange column chromatography to recover nyeloperoxidase.
COPYRIGHT: (C)1981,JPO&Japio
JP17210979A 1979-12-28 1979-12-28 Separation of myeloperoxidase from human nyelogenic leukocite Pending JPS5696694A (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP17210979A JPS5696694A (en) 1979-12-28 1979-12-28 Separation of myeloperoxidase from human nyelogenic leukocite
SE8005679A SE448882B (en) 1979-12-28 1980-08-12 PROCEDURE FOR THE EXTRACTION OF MYELOPEROXIDAS AND PHARMACEUTICAL COMPOSITION CONTAINING THE ENZYME
US06/179,783 US4306025A (en) 1979-12-28 1980-08-20 Method for recovering myeloperoxidase and pharmaceutical composition containing myeloperoxidase as major constituent
DE3031990A DE3031990C2 (en) 1979-12-28 1980-08-25 Obtaining myeloperoxidase and medicinal products containing this enzyme as the main component
FR8018581A FR2472609A1 (en) 1979-12-28 1980-08-27 PROCESS FOR SEPARATING MYELOPEROXIDASE AND PHARMACEUTICAL COMPOSITION CONTAINING MYELOPEROXIDASE
AT0436780A AT370997B (en) 1979-12-28 1980-08-28 METHOD FOR OBTAINING MYELOPEROXIDASE
IN758/CAL/81A IN153488B (en) 1979-12-28 1981-07-08
US06/297,418 US4379141A (en) 1979-12-28 1981-08-28 Method for recovering myeloperoxidase and pharmaceutical composition containing myeloperoxidase as major constituent
GB8233109A GB2108387B (en) 1979-12-28 1982-11-19 A pharmaceutical composition containing myeloperoxidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17210979A JPS5696694A (en) 1979-12-28 1979-12-28 Separation of myeloperoxidase from human nyelogenic leukocite

Publications (1)

Publication Number Publication Date
JPS5696694A true JPS5696694A (en) 1981-08-04

Family

ID=15935712

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17210979A Pending JPS5696694A (en) 1979-12-28 1979-12-28 Separation of myeloperoxidase from human nyelogenic leukocite

Country Status (1)

Country Link
JP (1) JPS5696694A (en)

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