JPS5655395A - Preparation of physiologically active substance - Google Patents

Preparation of physiologically active substance

Info

Publication number
JPS5655395A
JPS5655395A JP13154379A JP13154379A JPS5655395A JP S5655395 A JPS5655395 A JP S5655395A JP 13154379 A JP13154379 A JP 13154379A JP 13154379 A JP13154379 A JP 13154379A JP S5655395 A JPS5655395 A JP S5655395A
Authority
JP
Japan
Prior art keywords
factor
plasminogen
preparation
activating factor
molecular weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP13154379A
Other languages
Japanese (ja)
Other versions
JPS6213928B2 (en
Inventor
Akio Hasegawa
Sadayoshi Horiguchi
Mitsuru Shibukawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Chemical Industry Co Ltd
Original Assignee
Asahi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Industry Co Ltd filed Critical Asahi Chemical Industry Co Ltd
Priority to JP13154379A priority Critical patent/JPS5655395A/en
Priority to DE3015699A priority patent/DE3015699C2/en
Priority to GB8013755A priority patent/GB2051075B/en
Priority to FR8009443A priority patent/FR2454809A1/en
Priority to US06/143,680 priority patent/US4317882A/en
Publication of JPS5655395A publication Critical patent/JPS5655395A/en
Publication of JPS6213928B2 publication Critical patent/JPS6213928B2/ja
Granted legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Abstract

PURPOSE: To prepare the titled substance having high thrombolytic activity, in high concentration and yield, by separating from a solution obtained by culturing cells capable of producing plasminogen-activating factor, in the presence of a specific compound.
CONSTITUTION: Plasminogen-activating factor having a molecular weight of 45,000W68,000 is separated from a solution containing the factor and obtained by contacting (A) cells capable of producing plasminogen-activating factor and originated from human kidney or lung, with (B) a solution containing a nitrogen source, a carbon source, inorganic salts, and if necessary, organic nutrients. The separation is carried out in the presence of at least one metal-chelating agent (e.g. ethylenediaminetetraacetic acid). The chelating agent suppresses the decomposition and denaturation of the factor during the separation process, and hence ensures the preparation of the factor having uniform molecular weight, in high purity and concentration.
COPYRIGHT: (C)1981,JPO&Japio
JP13154379A 1979-04-26 1979-10-12 Preparation of physiologically active substance Granted JPS5655395A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP13154379A JPS5655395A (en) 1979-10-12 1979-10-12 Preparation of physiologically active substance
DE3015699A DE3015699C2 (en) 1979-04-26 1980-04-24 Manufacture of a plasminogen activator
GB8013755A GB2051075B (en) 1979-04-26 1980-04-25 Plasminogen activator
FR8009443A FR2454809A1 (en) 1979-04-26 1980-04-25 PROCESS FOR PRODUCING A PLASMINOGEN ACTIVATOR
US06/143,680 US4317882A (en) 1979-04-26 1980-04-25 Production of plasminogen activator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13154379A JPS5655395A (en) 1979-10-12 1979-10-12 Preparation of physiologically active substance

Publications (2)

Publication Number Publication Date
JPS5655395A true JPS5655395A (en) 1981-05-15
JPS6213928B2 JPS6213928B2 (en) 1987-03-30

Family

ID=15060527

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13154379A Granted JPS5655395A (en) 1979-04-26 1979-10-12 Preparation of physiologically active substance

Country Status (1)

Country Link
JP (1) JPS5655395A (en)

Also Published As

Publication number Publication date
JPS6213928B2 (en) 1987-03-30

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