JPS5633551A - Sensitized latex reagent for diagnosing staphylococcosis and method of detecting staphylococcal toxin alpha antibody using the same - Google Patents
Sensitized latex reagent for diagnosing staphylococcosis and method of detecting staphylococcal toxin alpha antibody using the sameInfo
- Publication number
- JPS5633551A JPS5633551A JP10921279A JP10921279A JPS5633551A JP S5633551 A JPS5633551 A JP S5633551A JP 10921279 A JP10921279 A JP 10921279A JP 10921279 A JP10921279 A JP 10921279A JP S5633551 A JPS5633551 A JP S5633551A
- Authority
- JP
- Japan
- Prior art keywords
- latex
- toxin
- particles
- specific gravity
- staphylococcosis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To detect the subject antibody easily and with high sensitivity, by bringing a latex reagent prepared by sensitizing high specific gravity latex particles with a staphylococcal α toxin into contact with a body fluid or its diluted solution and to observe a sedimentation pattern by a microtiter method.
CONSTITUTION: A staphylococcal α toxin is purified, and particles of high specific gravity latex having a specific gravity of 1.1 or more, or polymer latex such as chlorostyrene, vinyl chloride or the like are sensitized with this purified toxin. After the sensitization of latex particles, the latex particles are washed with an aqueous medium to eliminate surplus antigen. Then, the unsensitized surfaces of the latex particles are saturated with bovine serum albumin or the like, and subjected to a treatment for preventing nonspecific agglutination. The thus prepared latex reagent is brought into contact with a body fluid such as human or animal serum, or its diluted solution on a plate for microtiter, and a sedimentation pattern of latex is observed whereby the existence of the α toxin antibody is discriminated.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10921279A JPS5633551A (en) | 1979-08-29 | 1979-08-29 | Sensitized latex reagent for diagnosing staphylococcosis and method of detecting staphylococcal toxin alpha antibody using the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10921279A JPS5633551A (en) | 1979-08-29 | 1979-08-29 | Sensitized latex reagent for diagnosing staphylococcosis and method of detecting staphylococcal toxin alpha antibody using the same |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5633551A true JPS5633551A (en) | 1981-04-04 |
Family
ID=14504431
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10921279A Pending JPS5633551A (en) | 1979-08-29 | 1979-08-29 | Sensitized latex reagent for diagnosing staphylococcosis and method of detecting staphylococcal toxin alpha antibody using the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5633551A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0688824A (en) * | 1992-09-07 | 1994-03-29 | Toray Ind Inc | Examining method for infection with yellow staphylococus |
-
1979
- 1979-08-29 JP JP10921279A patent/JPS5633551A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0688824A (en) * | 1992-09-07 | 1994-03-29 | Toray Ind Inc | Examining method for infection with yellow staphylococus |
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