WO1991002590A3 - Immunoassay to detect pseudocercosporella antigen in cereal plants - Google Patents

Immunoassay to detect pseudocercosporella antigen in cereal plants Download PDF

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Publication number
WO1991002590A3
WO1991002590A3 PCT/US1990/004506 US9004506W WO9102590A3 WO 1991002590 A3 WO1991002590 A3 WO 1991002590A3 US 9004506 W US9004506 W US 9004506W WO 9102590 A3 WO9102590 A3 WO 9102590A3
Authority
WO
WIPO (PCT)
Prior art keywords
immunoassay
pseudocercosporella
antigen
detect
cereal plants
Prior art date
Application number
PCT/US1990/004506
Other languages
French (fr)
Other versions
WO1991002590A2 (en
Inventor
George Robinson Long
Robert Paul Ii Luoma
David William Saunders
James William Stave
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of WO1991002590A2 publication Critical patent/WO1991002590A2/en
Publication of WO1991002590A3 publication Critical patent/WO1991002590A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00—Other disintegrating devices or methods
    • B02C19/08—Pestle and mortar
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/14—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from fungi, algea or lichens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A rapid, sensitive and specific immunometric assay system is provided for determining the presence and/or concentration of antigens associated with infection of cereal plants by the fungal pathogen Pseudocercosporella herpotrichoides including a grinding apparatus for extracting such antigens in suitable liquid media. A polyclonal, monospecific antibody reagent is employed in a 'forward-sandwich' immunoassay for detection of polyvalent fungal antigens associated with the development of cereal eyespot disease.
PCT/US1990/004506 1989-08-24 1990-08-16 Immunoassay to detect pseudocercosporella antigen in cereal plants WO1991002590A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39826589A 1989-08-24 1989-08-24
US398,265 1989-08-24

Publications (2)

Publication Number Publication Date
WO1991002590A2 WO1991002590A2 (en) 1991-03-07
WO1991002590A3 true WO1991002590A3 (en) 1992-04-02

Family

ID=23574702

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/004506 WO1991002590A2 (en) 1989-08-24 1990-08-16 Immunoassay to detect pseudocercosporella antigen in cereal plants

Country Status (3)

Country Link
EP (1) EP0534963A1 (en)
AU (1) AU6417990A (en)
WO (1) WO1991002590A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9018689D0 (en) * 1990-08-24 1990-10-10 Dow Chemical Co Antigenic material,antibodies raised thereto,hybridomas for producing antibodies,and immunoassays incorporating such antibodies
JPH07506921A (en) * 1992-03-06 1995-07-27 ランバス・インコーポレーテッド Cache prefetching to minimize main memory access time and cache memory size in computer systems
USRE39879E1 (en) * 1992-03-06 2007-10-09 Rambus, Inc. Method of transferring data by transmitting lower order and upper order memory address bits in separate words with respective op codes and start information
US5355391A (en) * 1992-03-06 1994-10-11 Rambus, Inc. High speed bus system
WO1993018463A1 (en) * 1992-03-06 1993-09-16 Rambus, Inc. Method and circuitry for minimizing clock-data skew in a bus system
US5715407A (en) * 1992-03-06 1998-02-03 Rambus, Inc. Process and apparatus for collision detection on a parallel bus by monitoring a first line of the bus during even bus cycles for indications of overlapping packets
US5485490A (en) * 1992-05-28 1996-01-16 Rambus, Inc. Method and circuitry for clock synchronization
US5268639A (en) * 1992-06-05 1993-12-07 Rambus, Inc. Testing timing parameters of high speed integrated circuit devices
JP3279681B2 (en) * 1992-09-03 2002-04-30 株式会社日立製作所 Semiconductor device
US5558996A (en) * 1994-07-01 1996-09-24 Strategic Diagnostics Inc. Fungus extraction method, kit, and extraction solution
US20040241662A1 (en) * 2003-05-30 2004-12-02 Robey W. Wade Detecting microbial contamination in grain and related products
IE20030856A1 (en) * 2003-11-14 2005-06-15 Enfer Technology Ltd Sample homogeniser
CN113777064B (en) * 2021-09-10 2022-04-01 合肥工业大学 Kit and method for detecting in-vitro antioxidant activity of xylaria intracellular polysaccharide

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366930A (en) * 1981-04-27 1983-01-04 Trombetti Jr Albert V Tablet pulverizer
WO1988002613A1 (en) * 1986-10-13 1988-04-21 Willaume Jantzen Erik Grater
US4765549A (en) * 1986-05-15 1988-08-23 Renee Sherman Tablet pulverizer

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366930A (en) * 1981-04-27 1983-01-04 Trombetti Jr Albert V Tablet pulverizer
US4765549A (en) * 1986-05-15 1988-08-23 Renee Sherman Tablet pulverizer
WO1988002613A1 (en) * 1986-10-13 1988-04-21 Willaume Jantzen Erik Grater

Also Published As

Publication number Publication date
WO1991002590A2 (en) 1991-03-07
AU6417990A (en) 1991-04-03
EP0534963A1 (en) 1993-04-07

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