JPS5633549A - Blood cells sensitized with nicotinamide adenine dinucleotidase (nadase) for measuring hemolytic streptococcal nadase antibody and method of producing the same - Google Patents
Blood cells sensitized with nicotinamide adenine dinucleotidase (nadase) for measuring hemolytic streptococcal nadase antibody and method of producing the sameInfo
- Publication number
- JPS5633549A JPS5633549A JP10910579A JP10910579A JPS5633549A JP S5633549 A JPS5633549 A JP S5633549A JP 10910579 A JP10910579 A JP 10910579A JP 10910579 A JP10910579 A JP 10910579A JP S5633549 A JPS5633549 A JP S5633549A
- Authority
- JP
- Japan
- Prior art keywords
- blood cells
- nadase
- cells
- sensitized
- processed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To produce sensitized blood cells capable of performing detection of the subject antibody with high sensitivity and easily, by coupling hemolytic streptococcal nicotinamide adenine dinucleotidase (NADase) to red cells processed by a coupling agent.
CONSTITUTION: Live red cells or fixed red cells ar processed using a coupling agent such as tannic acid to obtain blood cells processed by the coupling agent. A solution containing NADase, which is separated and purified by a hemolytic streptococal cultivation filtrate, is brought into contact with the above-described processed blood cells to produce NADase-sensitized blood cells. In order to preserve these sensitized blood cells for a long period of time, fixed red cells are used as red cells, and the thus obtained sensitized blood cells are suspended in a preserving solution to be preserved, or preserved by freeze-drying the blood cells together with a medium containing a protecting agent. In the case of using the freeze-dried article, a diluting solution is added to the sensitized blood cells to prepare a diagnosis solution.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10910579A JPS5633549A (en) | 1979-08-29 | 1979-08-29 | Blood cells sensitized with nicotinamide adenine dinucleotidase (nadase) for measuring hemolytic streptococcal nadase antibody and method of producing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10910579A JPS5633549A (en) | 1979-08-29 | 1979-08-29 | Blood cells sensitized with nicotinamide adenine dinucleotidase (nadase) for measuring hemolytic streptococcal nadase antibody and method of producing the same |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5633549A true JPS5633549A (en) | 1981-04-04 |
Family
ID=14501697
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10910579A Pending JPS5633549A (en) | 1979-08-29 | 1979-08-29 | Blood cells sensitized with nicotinamide adenine dinucleotidase (nadase) for measuring hemolytic streptococcal nadase antibody and method of producing the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5633549A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2464301A1 (en) * | 1980-08-28 | 1981-03-06 | Seikagaku Kogyo Co Ltd | Streptococcal NADase antibody determn. - by latex agglutination using synthetic polymer latex particles coated with streptococcal Nadase |
JPS63148752U (en) * | 1987-03-20 | 1988-09-30 |
-
1979
- 1979-08-29 JP JP10910579A patent/JPS5633549A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2464301A1 (en) * | 1980-08-28 | 1981-03-06 | Seikagaku Kogyo Co Ltd | Streptococcal NADase antibody determn. - by latex agglutination using synthetic polymer latex particles coated with streptococcal Nadase |
JPS63148752U (en) * | 1987-03-20 | 1988-09-30 |
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